Investigation of serum beta-defensin-1 levels in bovine trichophytosis cases

Research (Published online: 24-09-2021)

26. Investigation of serum beta-defensin-1 levels in bovine trichophytosis cases

Aynur Simsek

Veterinary World, 14(9): 2508-2511

ABSTRACT

Background and Aim: Antimicrobial peptides are polypeptides that are a component of innate immunity and exhibit antifungal activity. This study aimed to investigate serum beta-defensin-1 levels in cattle diagnosed with trichophytosis, which is a zoonotic skin disease that affects several animal species.

Materials and Methods: A total of 23 young cattle, aged 2-4 months, of different breeds and sexes were selected. Of these, 16 cattle were clinically diagnosed with trichophytosis and seven were healthy.

Results: The mean serum beta-defensin-1 levels of the infected animals were lower than those of control animals, yet the difference between the two groups was not significant (p>0.05).

Conclusion: No significant alterations occurred in serum beta-defensin-1 levels of cattle with trichophytosis.

Keywords: beta-defensin-1, bovine, serum, trichophytosis.

Measuring the sustainability of beef supply chain with rapid appraisal for beef supply chain

Research (Published online: 23-09-2021)

25. Measuring the sustainability of beef supply chain with rapid appraisal for beef supply chain

Aries Susanty, Ratna Purwaningsih, Haryo Santoso, Anggun Novi Arista and Benny Tjahjono

Veterinary World, 14(9): 2488-2507

ABSTRACT

Background and Aim: Nationally, there has always been a gap between the demand for beef and its supply, although supply growth is proportional with demand growth and even exceeds it in some regions in Indonesia. This research study aims to measure the sustainability status of the beef supply chain and applies the developed measurement system to a specific beef supply chain by identifying suitable indicators and their scale. Moreover, this research study provides some recommendations for the improvement of the sustainability status of the beef supply chain.

Materials and Methods: In this research study, 11 and nine indicators were analyzed to assess the sustainability status of the beef supply chain at the farm and slaughterhouse chain levels. A rapid appraisal for beef supply chain was applied to rapidly assess the sustainability status of beef supply chains using Multidimensional Scaling (MDS). The Delphi method was utilized as an iterative process to collect data and obtain consensus of experts' judgments regarding the policies that should be implemented to improve the most sensitive indicator affecting the economic, social, and environmental dimensions.

Results: Analysis of ordination with MDS shows the regional sustainability index value for multidimensional approaches of beef cattle farms and beef slaughterhouses. The sustainability index value for beef cattle farms was 56.14 (moderately sustainable), 48.02 (fairly unsustainable), and 48.77 (fairly unsustainable) in Semarang, Sragen, and Boyolali, respectively. Moreover, the sustainability index value for beef slaughterhouses was 47.05 (fairly unsustainable), 54.83 (moderately sustainable), and 54.19 (moderately sustainable) in Semarang, Sragen, and Boyolali, respectively. Policy recommendation was focused on the basis of the results of leverage analysis, which highlighted the most indicative factor affecting sustainability for each dimension.

Conclusion: Measurement results revealed that the achievement of beef supply chain sustainability requires targeted efforts through the deployment of several policies as the current status of sustainability in beef farms and beef slaughterhouses was only inclined toward moderately sustainable and fairly unsustainable. Although all the surveyed regions in this study can meet the regional needs of beef meat on their own and even distribute the excess to other regions, none of the beef supply chains of the surveyed region indicated good sustainability.

Keywords: beef supply chain, indicators, rapid appraisal for beef supply chain, sustainability.

Sparganosis (Spirometra spp.) in Asian Water Monitor (Varanus salvator): A medical implications for veterinarians, breeders, and consumers

Research (Published online: 23-09-2021)

24. Sparganosis (Spirometra spp.) in Asian Water Monitor (Varanus salvator): A medical implications for veterinarians, breeders, and consumers

Aditya Yudhana, Ratih Novita Praja and Anjani Marisa Kartikasari

Veterinary World, 14(9): 2482-2487

ABSTRACT

Background and Aim: The high prevalence of sparganosis has been reported globally, especially in Asian countries where the majority of individuals consume raw meat from wild-caught reptiles. In Indonesia, similar cases regarding the high prevalence of sparganosis were recorded from wild reptiles such as snakes that utilized for culinary purposes, whereas, there are no data regarding other species such as water monitor lizard (Varanus salvator), which also provided as daily culinary with a high number of trades. Therefore, this study aims to investigate the prevalence of sparganosis in water monitor lizard (V. salvator), which is frequently utilized as culinary products in East Java Province, Indonesia.

Materials and Methods: A total of 313 living wild-caught and captive-bred Asian water monitor lizards were collected from the reptile markets and breeders. All samples were euthanized and observed for the presence of plerocercoid. Identification of the plerocercoid as larval infective stage was made using carmine staining method.

Results: The total prevalence of sparganosis was recorded at 69.64%. A total of 393 plerocercoids were collected in which divided 280 (71.24%) infecting muscles and 113 (28.75%) located in subcutaneous tissues.

Conclusion: To the best of our knowledge, this study not only recorded as the first evidence but also confirms the role of monitor lizards as sparganosis transmitters in Asia and reveals additional routes of sparganosis transmission in Indonesian reptiles. Local conservation laws should be strengthened to effectively control or ban wildlife trade in traditional markets. Moreover, public awareness regarding sparganosis as a neglected zoonotic disease should be applied to prevent disease transmission in Indonesia.

Keywords: infectious disease, sparganosis, Spirometra, Varanus salvator.

The potential of sperm bovine protamine as a protein marker of semen production and quality at the National Artificial Insemination Center of Indonesia

Research (Published online: 23-09-2021)

23. The potential of sperm bovine protamine as a protein marker of semen production and quality at the National Artificial Insemination Center of Indonesia

Berlin Pandapotan Pardede, Tulus Maulana, Ekayanti Mulyawati Kaiin, Muhammad Agil, Ni Wayan Kurniani Karja, Cece Sumantri and Iman Supriatna

Veterinary World, 14(9): 2473-2481

ABSTRACT

Background and Aim: Protamine (PRM) is the major protein in the sperm nucleus and plays an essential role in its normal function. Moreover, PRM has great potential as a protein marker of semen production and quality. This study aimed to assess the potential of sperm bovine PRM as a protein marker of semen production and quality in bulls at the National Artificial Insemination (AI) Center of Indonesia.

Materials and Methods: The semen production capacity of each bull was collected from frozen semen production data at the Singosari AI Center for 6 months, and was then divided into two groups (high and low). A total of 440 frozen semen straws from six Limousin (LIM), six Friesian Holstein (FH), six Peranakan Ongole (PO), and four Aceh bulls aged 4-5 years were used in the study. The frozen semen was used to measure the concentration of PRM1, PRM2, and PRM3 using the enzyme immunoassay method. The frozen semen was also used to assess the quality of the semen, including progressive motility (PM) through computer-assisted semen analysis, sperm viability through eosin–nigrosin analysis, and the DNA fragmentation index through Acridine Orange staining.

Results: PRM1 was significantly higher in all bull breeds included in the study (p<0.00), followed by PRM2 (p<0.00) and PRM3 (p<0.00). PRM1 significantly affected semen production in LIM, FH, PO, and Aceh bulls (p<0.05). Moreover, PRM2 significantly affected semen production only in FH and Aceh bulls (p<0.05), whereas PRM3 affected this parameter in PO and Aceh bulls exclusively (p<0.05). Consistently and significantly, PRM1 was positively correlated with the PM and viability of sperm and negatively associated with its DNA fragmentation in LIM, FH, PO, and Aceh bulls (p<0.05; p<0.01). The correlation analysis between PRM2 and PRM3 and semen quality parameters varied across all bull breeds; some were positively and negatively correlated (p<0.05; p<0.01), and some were not correlated at all.

Conclusion: PRM1 has excellent potential as a protein marker of semen production and quality in bulls at the National AI Center of Indonesia.

Keywords: bull, protamine, protein marker, semen production, semen quality.

Zoonotic diseases appeared to be a major hurdle to successful deer farming in Bangladesh

Research (Published online: 22-09-2021)

22. Zoonotic diseases appeared to be a major hurdle to successful deer farming in Bangladesh

Sajeda Sultana, Nazneen Sultana, Mahmuda Islam, Munmun Pervin, Md. Ariful Islam Khan and Mohammad Abu Hadi Noor Ali Khan

Veterinary World, 14(9): 2462-2472

ABSTRACT

Background and Aim: Due to the diversified lifestyle and fancy ecology associated with Chitra deer (Axis axis), deer farming has become popular in Bangladesh. Diseases may be the common constrain of successful deer farming. This study aims to investigate the pathological, bacteriological, and nucleic acid based technologies to identify specific causes of morbidity and mortality of captive deer.

Materials and Methods: Two deer farms and a park deer (designated as farm A, B, and C) entailing 87, 54, and 20 deer, respectively, showed illness and death constitute the study materials. A total of 42 deer died during this investigation. Following death, routine post-mortem examination, histopathology, impression smear staining, isolation, and identification of bacteria were carried out. Polymerase chain reaction (PCR) and reverse transcription PCR were carried out to safeguard the etiology.

Results: Clinically, farm A and B showed the acute phase of illness and park deer showed chronic illness. Case fatality rates were 90%, 92%, and 100% in farms A, B, and C deer, respectively. Pasteurella multocida and Streptococcus pneumoniae were identified from the visceral organs of farm A deer. Farm B deer was infected with Clostridium perfringens type A. Park deer was infected with Mycobacterium tuberculosis and hydatid cyst.

Conclusion: The infectivity in farm A deer was due to stress as induced by punishing weather. The infectivity in farm B deer was due to feeding a higher volume of protein in the diet. The park C deer may optate infection from companion man and animals living around. The diseases of captive deer identified mainly were zoonotic. It needs extensive veterinary services and specialized technologies to identify these diseases, monitor the infectivity and eliminate the public health important diseases at early onset.

Keywords: deer, enterotoxaemia, mycobacterium, pasteurella, zoonosis.

Effects of dietary supplementation of Vitamins E and C on oxidative stress induced by a Nigerian velogenic strain of the Newcastle disease virus (KUDU 113) in the brain and bursa of Fabricius of broiler chickens

Research (Published online: 21-09-2021)

21. Effects of dietary supplementation of Vitamins E and C on oxidative stress induced by a Nigerian velogenic strain of the Newcastle disease virus (KUDU 113) in the brain and bursa of Fabricius of broiler chickens

Obianuju Nkiruka Okoroafor, Temitope Mofoluso Ogunniran, Nkechi Harriet Ikenna-Ezeh, Ikechukwu John Udeani, Jacinta Ngozi Omeke, Wilfred Sunday Ezema and Boniface Anene

Veterinary World, 14(9): 2452-2461

ABSTRACT

Background and Aim: Newcastle disease (ND) is widely recognized as an extremely harmful and contagious disease of birds. Therefore, the present study aims to evaluate the effect of oxidative stress induced by the virulent ND virus (NDV) (KUDU 113) on the plasma, brain, bursa of Fabricius, NDV antibody response, and hematology as well as the ameliorative effect of the individual or combined use of Vitamins E and C on the clinical signs of NDV-infected chickens.

Materials and Methods: In this study, a total of 150 broiler chickens were included and divided into five groups: Group 1, nonsupplemented and unchallenged chickens (UCC); Group 2, nonsupplemented and challenged chickens (ICC); Group 3, Vitamin C-supplemented + challenged chickens; Group 4, Vitamin E-supplemented + challenged chickens; and Group 5, Vitamins E and C-supplemented + challenged chickens. Groups 3, 4, and 5 were supplemented with Vitamins E and C (33 and 400 mg/kg/day, respectively). Virus challenge was done with 0.1 ml of KUDU 113 7 days after the start of vitamin inclusion in their diet. Concentrations of glutathione (GSH), malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), and catalase (CAT) were analyzed in the plasma, brain, and bursa on days 0, 3, and 7 post-infection (pi) using the biochemical method. The blood samples were randomly collected from five chickens in each group for antibody response and hematological analyses on day 0 previtamin treatment and at 0, 3, 7, 10, 14, and 21 days pi (dpi).

Results: A significant increase in the concentrations of MDA and NO in the NDV-challenged chickens was observed when compared with the UCCs. Moreover, a significant decrease in GSH concentration was observed in the NDV-challenged chickens when compared with the UCCs. The activities of CAT and SOD were reduced markedly in the NDV-challenged chickens. Increases in the mean antibody titers were observed in the NDV-challenged group when compared with the UCCs from days 3 to 21 pi. The mortality rates of groups 1, 2, 3, 4, and 5 were 0%, 30%, 3.3%, 3.3%, and 26.6%, respectively.

Conclusion: The findings of this study suggest that KUDU 113 causes oxidative stress in the brain and bursa of Fabricius of chickens. Individual supplementation with Vitamin E or C was found to be more effective in ameliorating oxidative stress, improving the immune response, and reducing mortality in KUDU 113 infections than the combined supplementation of Vitamins C and E.

Keywords: hematologic parameters, Newcastle disease, oxidative stress, Vitamin C, Vitamin E antibody titer.

Comparison among TonoVet, TonoVet Plus, Tono-Pen Avia Vet, and Kowa HA-2 portable tonometers for measuring intraocular pressure in dogs

Research (Published online: 21-09-2021)

20. Comparison among TonoVet, TonoVet Plus, Tono-Pen Avia Vet, and Kowa HA-2 portable tonometers for measuring intraocular pressure in dogs

João Victor Goulart Consoni Passareli, Felipe Franco Nascimento, Giovana José Garcia Estanho, Claudia Lizandra Ricci, Glaucia Prada Kanashiro, Rogério Giuffrida and Silvia Franco Andrade

Veterinary World, 14(9): 2444-2451

ABSTRACT

Background and Aim: Tonometers are an important instrument for measuring intraocular pressure (IOP) in the diagnosis of glaucoma or uveitis. This study aimed to compare the accuracy of the main types of tonometers with different IOP measurement methodologies in dogs: TonoVet and TonoVet Plus (rebound), Tono-Pen Avia Vet (applanation), and Kowa HA-2 (Goldmann applanation).

Materials and Methods: IOP was measured in 152 eyes of 76 dogs. A postmortem study was performed by comparing manometry and tonometry values and calculating the correlation coefficient (r2), in vivo real IOP (manometry) among the tonometers was compared, and an outpatient study was conducted with healthy eyes and eyes with signs of glaucoma and uveitis.

Results: In the postmortem study, the values of r2 in descending order were Kowa (0.989), TonoVet Plus (0.984), TonoVet (0.981), and Tono-Pen Avia Vet (0.847). The IOP values in mmHg in the in vivo study were as follows: Aneroid manometer (16.8±2.5.7), TonoVet (18.1±2.9), TonoVet Plus (20.6±2.3), Tono-Pen Avia Vet (17.1±2.5), and Kowa (16.1±1.7); in outpatient clinics: TonoVet (16.8±3.8), TonoVet Plus (19.2±2.9), Tono-Pen Avia Vet (16.2±2.4), and Kowa (15.0±1.3); glaucoma: TonoVet (30.2±3.5), TonoVet Plus (35.0±6.1), Tono-Pen Avia Vet (29.5±4.2), and Kowa (23.9±5.0); and uveitis: TonoVet (14.2±1.4), TonoVet Plus (17.6±1.9), Tono-Pen Avia Vet (13.7±2.1), and Kowa (12.6±1.7).

Conclusion: There was a strong correlation between IOP values and manometry in all the tonometers. The highest values were obtained with TonoVet Plus and the lowest with Kowa HA-2. All tonometers accurately measured IOP in dogs, including the latest TonoVet Plus, which showed an excellent correlation coefficient.

Keywords: applanation tonometry, direct manometry, dogs, Goldmann tonometry, rebound tonometry.