Optimal therapeutic adropin dose intervention in mice and rat animal models: A systematic review

Research (Published online: 04-06-2021)

4. Optimal therapeutic adropin dose intervention in mice and rat animal models: A systematic review

Foad Alzoughool and Mohammad Borhan Al-Zghoul

Veterinary World, 14(6): 1426-1429

ABSTRACT

Background and Aim: Adropin is a hormone encoded by the Enho gene, which is associated with energy homeostasis. Preclinical studies using animal models have shown that adropin plays a role in enhancing glucose homeostasis and dyslipidemia. Lately, several studies on animal models have been performed to examine the therapeutic and pathophysiological effects of adropin in many disorders. The aim of this systematic review was to identify the ideal adropin dose in mice and rat animal models.

Materials and Methods: We systematically searched PubMed, Science Direct, and Scopus databases from 2008 to 2020. The terms used in the search were "adropin," "adropin doses in animal models," "glucose homeostasis related to adropin," and "adropin therapeutic effects on rats and mice." Articles that included non-adropin doses, in vitro studies, and factors affecting adropin levels were excluded from the study.

Results: Of the total 179 qualified studies, six studies were included. We found that a daily injection of 450 nmol/kg of adropin for 3 days might be considered the optimum dose of effect in mice, whereas injection of 2.1 μg/kg once a day for 10 successive days might be the optimal effective dose in rats.

Conclusion: Additional investigations are needed to determine the optimum dose of adropin to be used as a therapeutic intervention depending on the animal model.

Keywords: adropin, animal model, dyslipidemia, glucose homeostasis, systematic review, therapeutic dose.

First molecular confirmation of Coenurus cerebralis in sheep and goats with neurological behaviors in Iraq

Research (Published online: 03-06-2021)

3. First molecular confirmation of Coenurus cerebralis in sheep and goats with neurological behaviors in Iraq

Eva Aisser Ajaj, Hadeel Asim Mohammad, and Hasanain A. J. Gharban

Veterinary World, 14(6): 1420-1425

ABSTRACT

Background and Aim: Coenurus cerebralis is the intermediate stage of the canine cestode, which infect sheep and goats, resulting mainly in neurological signs and causing direct and indirect economic losses. This study aimed to demonstrate the existence of C. cerebralis and to elucidate the role of this parasite in inducing neurological behaviors in sheep and goats.

Materials and Methods: On the basis of historical data on neurological signs, we subjected 76 animals (49 sheep and 27 goats) of different ages, sexes, and geographical areas for molecular examination of their blood samples using the polymerase chain reaction assay.

Results: Of the 76 animals, 23.68% tested positive for C. cerebralis infection. We found significant increases in infection (p<0.05) in sheep (26.53%) more than in goats (18.52%). Circling movement was prevalent significantly among both C. cerebralis-positive sheep and goats. The Nineveh region had a significant (p<0.05) increase in positive sheep and goats, and the sheep of all study regions were infected significantly (p<0.05) more than were the goats. We found no significant (p>0.05) variation between sheep ages ≥1-3 years and ≥3 years; however, both groups had a significantly (p≤0.043) higher positivity rate than did sheep ages <1 year. The findings of sheep ages <1 year and ≥1-3 years were significantly higher than those of the goats, but not for goats ages ≥3 years. Female sheep and goats showed a significant increase in positivity versus that for the males.

Conclusion: To the best of our knowledge, this study is the first report in Iraq targeting detection of C. cerebralis in sheep and goats with neurological behaviors; therefore, additional studies involving different animals in other regions using molecular techniques are needed.

Keywords: definitive, intermediate host, Iraq, polymerase chain reaction, Taenia multiceps.

Effects of Gossypium spp., Balanites aegyptiaca, and Sesamum indicum seeds oils on quality of chilled and frozen-thawed ram semen

Research (Published online: 03-06-2021)

2. Effects of Gossypium spp., Balanites aegyptiaca, and Sesamum indicum seeds oils on quality of chilled and frozen-thawed ram semen

Adamou Akourki, Arantxa Echegaray, Orlando Perdomo, Nicolas Angel Escartin and Marta Guillén

Veterinary World, 14(6): 1412-1419

ABSTRACT

Background and Aim: Essential oils found frequently in plants are well known for their activities against bacteria, viruses, and fungi, and antioxidant properties. This study aimed to analyze egg yolk replacement by seed oils of Gossypium spp. (cotton), Balanites aegyptiaca (desert date), and Sesamum indicum (sesame) in semen extender, on ram sperm quality chilled at 4°C and frozen-thawed.

Materials and Methods: Ejaculates were collected from adult rams and refrigerated at 4°C in a Tris-based extender containing 1.25%, 2.5%, 5%, and 10% of Gossypium spp., B. aegyptiaca, and S. indicum seed oils, to evaluate which were the two best extenders for comparison with BIOXcell, a commercial extender for deep freezing ram semen.

Results: The data showed that sperm movements analyzed by the CASA system were faster in extenders supplemented with 2.5-5% of cottonseed oil and 1.25-10% of sesame oil, whereas in the extender containing B. aegyptiaca oil, all seminal parameters studied had the worst values. During the sperm-freezing process, 5% of cottonseed oil and 5% sesame seed oil were selected from the first study, with sesame oil reaching the best sperm quality. Thus, sperm motility and velocity were 44.14±13.99%, 24.44±12.6%, and 25.92±11.50%; and 20.26±9.56%, 8.76±6.38%, and 9.42±5.40%, respectively, for sesame oil, cottonseed oil, and BIOXcell.

Conclusion: In summary, 2.5-10% of cottonseed oil and 1.25-10% of sesame seed oil can replace egg yolk in a Tris–egg yolk–based extender. Moreover, a Tris-based extender supplemented with 5% sesame seed oil could be an alternative for deep freezing ram semen, even though these results need to be confirmed with semen collected from rams with appropriate sexual rest.

Keywords: Balanites aegyptiaca, Gossypium spp., ram, semen freezing, Sesamum indicum.

Effect of dietary tannins on the performance, lymphoid organ weight, and amino acid ileal digestibility of broiler chickens: A meta-analysis

Research (Published online: 01-06-2021)

1. Effect of dietary tannins on the performance, lymphoid organ weight, and amino acid ileal digestibility of broiler chickens: A meta-analysis

Cecep Hidayat, Agung Irawan, Anuraga Jayanegara, Muhammad Miftakhus Sholikin, Tri Rachmanto Prihambodo, Yulianri Rizki Yanza, Elizabeth Wina, Sadarman Sadarman, Rantan Krisnan and Isbandi Isbandi

Veterinary World, 14(6): 1405-1411

ABSTRACT

Background and Aim: Tannins are functional secondary metabolites that may provide benefits to ruminants. However, to date, their effects on broiler chickens remain inconclusive. This study aimed to evaluate the effectiveness of dietary tannin levels on the performance, body organs, and amino acid (AA) digestibility of broiler chickens using a meta-analysis.

Materials and Methods: After verification and evaluation, a total of 22 articles were included in the present study. All data regarding dietary tannin dosages, performance, digestibility, and gastrointestinal physiology of broiler chickens were tabulated into a database. The database data were then statistically analyzed using mixed models, with tannin dose as a fixed effect and study as a random effect.

Results: High levels of dietary tannins negatively affected the average daily gain and average daily feed intake of broiler chickens according to linear patterns (p<0.001). In addition, dietary tannins decreased drumstick and liver weights, as well as bursa of Fabricius and spleen weight (p<0.05). Meanwhile, other carcass traits (i.e., thigh, wings, and body fat) were not influenced by dietary tannins. Regarding AA digestibility, high dietary tannin concentrations induced negative responses on isoleucine, leucine, and methionine digestibility (p<0.05).

Conclusion: Dietary tannins appear to have a negative effect on broiler performance, lymphoid organ weight, and AA ileal digestibility. Hence, the addition of tannins to broiler diets is not recommended.

Keywords: amino acid ileal digestibility, broiler chicken, meta-analysis, tannin.

The combination of Elephantopus scaber and Sauropus androgynus promotes erythroid lineages and modulates follicle-stimulating hormone and luteinizing hormone levels in pregnant mice infected with Escherichia coli

Research (Published online: 31-05-2021)

43. The combination of Elephantopus scaber and Sauropus androgynus promotes erythroid lineages and modulates follicle-stimulating hormone and luteinizing hormone levels in pregnant mice infected with Escherichia coli

Muhammad Sasmito Djati, Yuyun Ika Christina and Muhaimin Rifa'i

Veterinary World, 14(5): 1398-1404

ABSTRACT

Background and Aim: Escherichia coli infection produces an adverse effect on the erythrocyte lineage and hormone levels during pregnancy. This study aimed to evaluate the effects of Elephantopus scaber (ES) and Sauropus androgynus (SA) in combination on circulating follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels and erythropoiesis changes in E. coli-infected pregnant mice.

Materials and Methods: Female Balb/c mice were mated with normal male mice and pregnancies were identified by the formation of vaginal plugs. Twenty-eight pregnant mice were divided randomly into seven groups: A control group (N), E. coli-infected pregnant mice (K+), and infected pregnant mice received the following five treatments: (1) Only ES; (2) ESSA1 (75:25); (3) ESSA2 (50:50); (4) ESSA3 (25:75); and (5) only SA, beginning from the 1st to the 16th day of pregnancy. Pregnant mice were infected with 107 CFU/mL of E. coli on day 4. Blood serum was collected on days 8, 12, and 16 of pregnancy and LH and FSH levels were measured by enzyme-linked immunosorbent assay. Bone marrow was isolated to determine the relative number of TER-119+VLA4+ and TER-119+CD34+ using flow cytometry.

Results: The ESSA1 and SA groups exhibited a marked increase in LH levels. The combination of ES and SA administered at a 25:75 ratio (ESSA3) altered FSH levels and the relative number of TER-119+VLA4+ in infected pregnant mice. Combined with SA at an equal ratio (50:50), ESSA2 group exhibited a significant increase in the expression of TER119+CD34+ compared with the other treatment groups.

Conclusion: ES and SA combined at a ratio of 25:75 exhibited optimal results in altering hormonal and erythropoiesis in infected pregnant mice.

Keywords: Elephantopus scaber, erythropoiesis, follicle-stimulating hormone, luteinizing hormone, Sauropus androgynus.

Genetic diversity among two native Indian chicken populations using cytochrome c oxidase subunit I and cytochrome b DNA barcodes

Research (Published online: 30-05-2021)

42. Genetic diversity among two native Indian chicken populations using cytochrome c oxidase subunit I and cytochrome b DNA barcodes

Ankit R. Dave, Dhaval F. Chaudhary, Pooja M. Mankad, Prakash G. Koringa and D. N. Rank

Veterinary World, 14(5): 1389-1397

ABSTRACT

Background and Aim: India has large varieties (recognized, unrecognized) of native chickens (Desi) scattered throughout the country, managed under scavenging system different from commercial chicken breeds. However, they are less investigated for genetic diversity they harbor. The present study was planned to evaluate genetic diversity among two native chicken populations of North Gujarat (proposed Aravali breed) and South Gujarat (Ankleshwar breed). Aravali chicken, a distinct population with unique characters different from the registered chicken breeds of India is under process to be registered as a new chicken breed of Gujarat, India.

Materials and Methods: Two mitochondrial markers, namely, cytochrome c oxidase subunit I (COX I) and cytochrome b (Cyt b) genes were studied across 10 birds from each population. Methodology included sample collection (blood), DNA isolation (manual), polymerase chain reaction amplification of mitochondrial genes, Sanger sequencing, and purification followed by data analysis using various softwares.

Results: Haplotype analysis of the COX I gene unveiled a total eight and three haplotypes from the Aravali and Ankleshwar populations, respectively, with haplotype diversity (Hd) of 92.70 % for the Aravali and 34.50% for the Ankleshwar breed. Haplotype analysis of the Cyt b gene revealed a total of four haplotypes from the Aravali population with 60% Hd and no polymorphism in Ankleshwar breed. The phylogenetic analysis uncovered Red Jungle Fowl and Gray Jungle Fowl as prime roots for both populations and all domestic chicken breeds.

Conclusion: Study findings indicated high genetic variability in Aravali chicken populations with COX I mitochondrial marker being more informative for evaluating genetic diversity in chickens.

Keywords: Cytochrome b, Cytochrome oxidase, genetic diversity, mitochondrial DNA, phylogenetics, poultry.

The epizootic situation of cattle moraxellosis in several economic entities of the Republic of Kazakhstan

Research (Published online: 30-05-2021)

41. The epizootic situation of cattle moraxellosis in several economic entities of the Republic of Kazakhstan

N. P. Ivanov, F. A. Bakiyeva, A. M. Namet, R. S. Sattarova, B. Zh. Issakulova and N. Zh. Akmyrzayev

Veterinary World, 14(5): 1380-1388

ABSTRACT

Background and Aim: Infectious bovine keratoconjunctivitis (IBK; conjunctivitis) is a widespread eye disease in cattle. In 1960, the Gram-negative bacillus Moraxella bovis was discovered as one of IBK's etiological causal agents. This study aimed to clarify the epidemiological (epizootic) situation of cattle moraxellosis in the Republic of Kazakhstan. The study also maps the quantitative and qualitative indicators of the epidemiological (epizootic) process to develop more effective antiepizootic measures.

Materials and Methods: We clinically examined both imported and local cattle species based on existing epidemiological (epizootological) units of economic entities in Kazakhstan. Then, we selected biomaterials for laboratory tests to screen for moraxellosis.

Results: We clarified the epizootic situation for moraxellosis of cattle in Kazakhstan using data from the Meat Union of Kazakhstan, veterinary reports from the Veterinary Control and Surveillance Committee of the Ministry of Agriculture, Kazakhstan, and our results, obtained during visits to farms in the various regions of the republic.

Conclusion: Based on the data of the conducted studies, we developed epizootic visualization maps with quantitative indicators of the cattle moraxellosis epizootic-transfer processes within various regions of Kazakhstan for 2019. The data obtained from studies of the properties of the isolated cultures compared with the characteristics of reference strains gave grounds to classify them as belonging to the Moraxella genus.

Keywords: distribution, epizootology, import of livestock, moraxellosis, pathogen.