Effects of oregano (Lippia origanoides) essential oil supplementation on the performance, egg quality, and intestinal morphometry of Isa Brown laying hens

Research (Published online: 09-03-2021)

7. Effects of oregano (Lippia origanoides) essential oil supplementation on the performance, egg quality, and intestinal morphometry of Isa Brown laying hens

Swanny Y. Ramirez, Lina M. Peñuela-Sierra and Maria A. Ospina

Veterinary World, 14(3): 595-602

ABSTRACT

Background and Aim: The use of antibiotics as growth promoters in the feed of poultry, has contributed to an increase in the antimicrobial resistance of foodborne pathogens worldwide. Hence, the development of new effective alternatives to antibiotics that do not hinder productivity is imperative. For this, the aim of the present study was to determine whether oregano essential oil (OEO) extracted from Lippia origanoides is a suitable alternative to growth-promoting antibiotics (GPAs) for improving the performance, egg quality, and intestinal morphometry of ISA Brown laying hens.

Materials and Methods: A total of ninety-six 70-week-old ISA Brown laying hens were randomly assigned to four treatment groups with four replicates per treatment and six hens per replicate. The treatments consisted of four different diets that were formulated according to the nutritional requirements of the genetic line and the production phase with and without the addition of GPA and OEO: NC, which did not contain OEO or GPA; GPA, which included 50 ppm zinc bacitracin as a GPA; 80OEO, which included 80 ppm OEO and no GPA; and 150OEO, which included 150 ppm OEO and no GPA.

Results: All of the OEO and GPA treatment groups had a better feed conversion ratio than the NC group. However, the addition of 150 ppm OEO to the diet improved the percentage egg production and egg mass, as well as the external and internal quality of the egg compared with the other treatments. In addition, both the 80OEO and 150OEO treatments improved the yolk color, shell thickness, and shell color, as well as parameters related to the intestinal morphometry compared with the NC group.

Conclusion: The findings of this study indicate that 150 ppm OEO can be used as a substitute for GPA to improve the performance, egg quality, and parameters related to the intestinal morphometry of ISA Brown laying hens.

Keywords: egg quality, growth promoter antibiotic, intestinal morphometry, oregano essential oil, performance.

Pretreatment with Salvadora persica L. (Miswak) aqueous extract alleviates paracetamol-induced hepatotoxicity, nephrotoxicity, and hematological toxicity in male mice

Research (Published online: 08-03-2021)

6. Pretreatment with Salvadora persica L. (Miswak) aqueous extract alleviates paracetamol-induced hepatotoxicity, nephrotoxicity, and hematological toxicity in male mice

Mohd Alaraj, Tolgahan Acar, Irena Kosinska, Bahaa Al-Trad, Ammar M. Almaaytah, Mohamed J. Saadh, Mohammed A. Qumani, Shahid M. Syed, Khalil I. Altaif and Hossain Ashfaque

Veterinary World, 14(3): 589-594

ABSTRACT

Background and Aim: Paracetamol (PCM) ingestion is one of the most frequent global causes of toxicity. Salvadora persica L. is a plant that among many other effects exhibits potent antioxidant, anti-inflammatory, antimicrobial, and anticancer effects. In this study, we investigated the possible protective effect of S. persica aqueous extract in the PCM overdose-induced liver and kidney injury and hematological changes in a mice model.

Materials and Methods: Mice were given PCM with and without S. persica pretreatment. Blood cell counts and liver and kidney function biomarkers were measured. Liver and kidney samples were histologically examined.

Results: A single overdose of PCM caused significant elevations of alanine and aspartate transaminases, alkaline phosphate, bilirubin, urea, uric acid, and creatinine compared with the control group. In addition, PCM toxicity significantly lowered red blood cell count but insignificantly increased both white blood cell and platelet counts in comparison to the control mice. Pretreatment with S. persica significantly prevented PCM-induced changes in hepatic and renal biomarkers. S. persica also caused marked reversal of hematological changes. Histologically, the liver and kidney showed inflammation and necrosis after PCM treatment, which were significantly reduced in mice pretreated with S. persica.

Conclusion: Taken together, S. persica significantly inhibited PCM-induced renal, hepatic, and hematological toxicity, pointing to its possible use in the treatment of liver and renal disorders.

Keywords: hematology, kidney, liver, mice, paracetamol, Salvadora persica L.

Accuracy and precision guidelines for optimal breeding time in bitches using in-house progesterone measurement compared with chemiluminescent microparticle immunoassay

Research (Published online: 08-03-2021)

5. Accuracy and precision guidelines for optimal breeding time in bitches using in-house progesterone measurement compared with chemiluminescent microparticle immunoassay

Nutnaree Kunanusont, Darsaniya Punyadarsaniya and Sakchai Ruenphet

Veterinary World, 14(3): 585-588

ABSTRACT

Background and Aim: The concentration of serum progesterone is commonly used to determine the optimal mating time in bitches, and to diagnose reproductive-related abnormalities. This study aims to compare the serum progesterone results obtained by rapid fluorescence immunochromatography assay (RFICA) with those obtained by chemiluminescent microparticle immunoassay (CMIA) from the same serum samples to develop a standard guideline for optimal breeding time.

Materials and Methods: Serum progesterone levels were measured in 124 bitches using RFICA and CMIA. Simple linear regression and correlation analyses were performed to analyze the data. The percentage difference between the maximum and minimum progesterone values in the same serum sample in the same assay was compared using Wilcoxon's rank-sum test.

Results: The present study showed a strong linear dependence of the results obtained by RFICA on those obtained by CMIA as R2=0.8976, with regression coefficient of 0.9474 and p<0.05, including the regression model was CMIA = (0.9483 × RFICA) - 0.761. Moreover, five critical measurement times during estrous in bitches showed statistically significant differences (p<0.05), except at the fertilizable period, which showed a non-significant difference (p>0.05).

Conclusion: This study demonstrated that it is presumably acceptable to use the RFICA and CMIA methods interchangeably for quality progesterone measurements in serum samples from bitches. However, when considering the use of the RFICA method, it is advisable to carefully interpret the results and follow the interpretation guidelines. Finally, RFICA in the present study provides a reliable and convenient option for veterinarian practitioners to measure canine progesterone levels in-house.

Keywords: bitches, chemiluminescent microparticle immunoassay, optimal breed timing, progesterone, rapid fluorescence immunochromatography assay.

Diversity and prevalence of gastrointestinal parasites with zoonotic potential of Green Monkeys in Bandia Reserve in Senegal

Research (Published online: 06-03-2021)

8. Diversity and prevalence of gastrointestinal parasites with zoonotic potential of Green Monkeys in Bandia Reserve in Senegal

Kacou Martial N'da, Laibané Dieudonné Dahourou, Oubri Bassa Gbati and Rianatou Bada Alambedji

International Journal of One Health, 7(1): 65-69

ABSTRACT

Background and Aim: Parasitic and infectious diseases are ubiquitous threats to primate and human populations. This study was carried out to study the diversity and frequency of gastrointestinal parasites with zoonotic potential in green monkeys in the Bandia Reserve located in Senegal.

Materials and Methods: For this study, 164 stool samples of Chlorocebus aethiops sabaeus (green monkey) were collected from October to November 2018 from Bandia Reserve. Stool samples were processed using the merthiolate-iodine-formalin staining technique and the modified Ritchie method. The slides were examined under a microscope and the identification of parasites was based on the morphology of protozoan cysts and helminth eggs. The analysis of data was conducted using R version 3.4.3 with p=0.05.

Results: A total of six species of parasites were found, including five protozoa (Entamoeba coli, Entamoeba histolytica/ dispar, Entamoeba hartmanni, Endolimax nana, and Iodamoeba butschlii) and one nematode (Strongyloides spp.). The overall prevalence of gastrointestinal parasite was 76.2% (95% CI: 69.7%-82.7%). Two parasites with zoonotic potential, mainly E. histolytica/dispar with a prevalence of 13.4% (95% CI: 8.2%-18.6%) and Strongyloides spp. with a prevalence of 6.7% (95% CI: 2.9%-10.5%), were found.

Conclusion: This study indicated that the monkeys of the Bandia Reserve are infested by zoonotic parasites and can, therefore, ensure transmission to visitors to the Reserve. The parasitological data that we report are the first available for these species of monkeys in the Bandia Reserve.

Keywords: Bandia, Chlorocebus aethiops sabaeus, gastrointestinal parasites, Senegal, zoonotic parasites.

Wildlife-pet markets in a one-health context

Research (Published online: 06-03-2021)

7. Wildlife-pet markets in a one-health context

Clifford Warwick and Catrina Steedman

International Journal of One Health, 7(1): 42-64

ABSTRACT

Background and Aim: Wildlife markets are centers of trade involving live animals and their derivatives from wild-caught and captive-bred non-domesticated animals, including for the culinary, fashion, traditional medicine, curio, and pet sectors. These markets occur in Southeast Asia, India, North America, Latin America, Europe, Africa, and elsewhere. This study aims to address a diversity of related issues that have a one-health bearing while focusing on wildlife markets in relation to the pet trade. Across relevant regions and countries, all major animal classes are traded at wildlife-pet markets. Wildlife markets, in general, are considered distinct from so-called "wet markets" at which domesticated animals, fish, and other "seafood" are offered only for consumption. Several aspects of wildlife markets have attracted scientific and popular scrutiny, including animal welfare concerns, species conservation threats, legality, ecological alteration, introduction of invasive alien species, presence of undescribed species, and public and agricultural animal health issues.

Materials and Methods: Onsite inspections were conducted for markets in the United States, Spain, Germany, The Netherlands, and the UK, as well as observational research of visual imagery of market conditions, and we compared these conditions with evidence-based standards for animal welfare and public health management.

Results: Wildlife markets globally shared common similar structures and practices including the presence of sick, injured, or stressed animals; mixing of animals of uncertain origin and health state; and no specific or any hygiene protocols, with issues of animal welfare, public health and safety, agricultural animal health, and other one-health concerns being inherently involved.

Conclusion: We conclude that wildlife markets are incompatible with responsible standards and practices, and we recommend that such events are banned globally to ameliorate inherent major problems.

Keywords: animal welfare, disease, exotic pet, one-health, pathogen, public health, wildlife market, zoonoses.

Phenotypic detection and genotyping of Clostridium perfringens associated with enterotoxemia in sheep in the Qassim Region of Saudi Arabia

Research (Published online: 06-03-2021)

4. Phenotypic detection and genotyping of Clostridium perfringens associated with enterotoxemia in sheep in the Qassim Region of Saudi Arabia

Fehaid Alsaab, Ali Wahdan and Elhassan M. A. Saeed

Veterinary World, 14(3): 578-584

ABSTRACT

Background and Aim: Enterotoxemia caused by Clostridium perfringens toxinotypes is an often fatal disease of sheep of all ages, with a substantial economic loss to the sheep industry. This study was conducted to isolate C. perfringens from suspected cases of enterotoxemia in sheep in the central part of the Qassim Region, Saudi Arabia, and to determine the prevalent toxinotype by detecting alpha (cpA), beta (cpB), and epsilon (etX) toxin genes, which might help control this disease locally.

Materials and Methods: A total of 93 rectal swabs and intestinal content samples were collected from diseased and animals suspected of having died of enterotoxemia in early 2020. Samples were subjected to bacteriological examination, biochemical analysis of isolates by VITEK 2, and molecular toxinotyping of isolates by LightCycler® real-time polymerase chain reaction (RT-PCR).

Results: Our results revealed that only 14 isolates were confirmed by VITEK 2 as being C. perfringens, with excellent identification (probability of 95% and 97%). According to the toxinotyping of isolates by RT-PCR, all 14 isolates possessed both the cpA and etX toxin genes, while the cpB toxin gene was not detected in any of the isolates.

Conclusion: Our findings demonstrated that C. perfringens type D was the only toxinotype found in the central part of the Qassim Region in 2020; moreover, according to the culture method, only 15% (14/93) of the suspected cases of enterotoxemia were confirmed to be caused by C. perfringens infection, which highlighted the importance of clinical and laboratory differential diagnosis of enterotoxemia in sheep.

Keywords: Clostridium perfringens toxinotypes, enterotoxaemia, Qassim Region, real-time polymerase chain reaction, sheep, VITEK 2.

Histopathological evaluation of Senecio rhizomatus Rusby in 7,12-dimethylbenz(α) anthracene-induced breast cancer in female rats

Research (Published online: 06-03-2021)

3. Histopathological evaluation of Senecio rhizomatus Rusby in 7,12-dimethylbenz(α) anthracene-induced breast cancer in female rats

Jorge Luis Arroyo-Acevedo, Oscar Herrera-Calderon, Juan Pedro Rojas-Armas, Roberto Chávez-Asmat, James Calva and Tapan Behl

Veterinary World, 14(3): 569-577

ABSTRACT

Background and Aim: Senecio rhizomatus Rusby (SrR) is a medicinal plant of the Asteraceae family and traditionally consumed as infusion in the Andean region from Peru for inflammatory disorders. This study aimed to determine the histopathological changes afforded by SrR in 7, 12-dimethylbenz[α]anthracene (DMBA)-induced breast cancer (BC) in rats.

Materials and Methods: An ethanolic extract of SrR aerial parts was prepared by maceration with 96% ethanol, and the chemical components were identified by gas chromatography coupled to mass spectrometry; the antioxidant activity was determined by 1,1-diphenyl-2-picril-hidrazil (DPPH) assay; and the acute toxicity was assessed according to the OCED 423 guidelines. In a pharmacological study, 30 female Holztman rats were distributed randomly into five groups, as follows. Group I: Negative control (physiological serum, 2 mL/kg); Group II. DMBA (80 mg/Kg body weight); and Groups III, IV, and V: DMBA + ethanol extract of SrR at doses of 10, 100, and 200 mg/kg, respectively.

Results: The antioxidant activity of the SrR extract against DPPH was 92.50% at 200 μg/mL. The oral administration of SrR at doses of 50, 300, 2000, and 5000 mg/kg did not show any clinical evidence of toxicity or occurrence of death. The groups that received SrR presented a lower frequency of tumors and a cumulative tumor volume compared with the DMBA group (p<0.05); the DMBA group exhibited a higher incidence of necrosis and moderate mitosis, up to 66.67% and 100.00%, respectively. Finally, infiltrating carcinoma with extensive tumor necrosis was evidenced.

Conclusion: In experimental conditions, the ethanolic extract of SrR had a protective effect in DMBA-induced BC in female rats. Furthermore, the antioxidant activity of its main phytochemicals could be responsible for the effect observed, and SrR seems to be a safe extract in the preclinical phase.

Keywords: breast cancer, carcinogenic, experimental pharmacology, phytotherapy, Senecio rhizomatus.