Wednesday, 13 January 2021

G1 point mutation in growth differentiation factor 9 gene affects litter size in Sudanese desert sheep

Research (Published online: 13-01-2021)
14. G1 point mutation in growth differentiation factor 9 gene affects litter size in Sudanese desert sheep
Amani Z. Abdelgadir, Lutfi M. A. Musa, Khaleel I. Jawasreh, Aubai. O. Saleem, Faisal El-Hag and Mohamed-Khair A. Ahmed
Veterinary World, 14(1): 104-112

ABSTRACT

Background and Aim: Sudanese desert sheep encompass different sheep breeds named according to the different Sudanese tribes that rear them such as the Dubasi, Shugor, and Watish sheep. The objectives of this study were to screen for G1 point mutation in the polymorphic growth differentiation factor 9 (GDF9) gene, investigate its association with litter size, and construct the phylogeny of the different tribal breeds that belong to the Sudanese Desert sheep tribal types.

Materials and Methods: Genomic DNA was extracted from whole blood of three tribal Desert sheep breeds (Dubasi, Watish, and Shugor) using the guanidine chloride method. Polymerase chain reaction-restriction fragment length polymorphism with HhaI restriction enzyme and sequencing techniques was used for genotyping the GDF9 locus for possible mutations associated with litter size in the three desert sheep tribal types.

Results: G1 mutation in GDF9 caused the replacement of Arginine by Histidine at residue 87. The wild type allele (A) had the highest frequency, whereas the mutant type allele (a) had the lowest in all the sequenced subtypes. The genotype frequencies of the wild type ewes (AA) were higher than the heterozygous (Aa) and the mutant type (aa) frequencies in the three studied desert sheep types. No significant differences were found in the allele frequency between the three tribal types. Litter size was significantly influenced by the genotypes of GDF9 gene, parities, and subtypes (p≤0.01, 0.01, and 0.05, respectively). In the Watish sheep type, heterozygous sheep in their second parity recorded the highest litter size. Sequence alignment of GDF9 gene samples with the database entry indicated that all three tribal types were similar and identical to the reference sequence. The phylogenetic tree revealed that Shugor is the common ancestor of the studied types and Watish is more closely related to Shugor than Dubasi. This result mi ght partly explain the lower reproductive performance of Dubasi compared to Watish and Shugor.

Conclusion: The presence of one copy of GDF9 gene increased litter size in the studied Sudanese Desert sheep. This locus may be used as a biomarker for litter size improvement through genotypic selection and allele or gene introgression.

Keywords: common ancestor, growth differentiation factor 9 gene, similarity and identity, Sudanese sheep.



Assessment of novel probiotic strains on growth, hematobiochemical parameters, and production costs of commercial broilers in Bangladesh

Research (Published online: 13-01-2021)
13. Assessment of novel probiotic strains on growth, hematobiochemical parameters, and production costs of commercial broilers in Bangladesh
S. M. Tanjil Shah, Md. Tanvir Islam, Rakiba Zabin, Pravas Chandra Roy, Nigar Sultana Meghla and Iqbal Kabir Jahid
Veterinary World, 14(1): 97-103

ABSTRACT

Background and Aim: The use of antibiotic growth promoters (AGPs) in the poultry industry has raised concern because of their potential harm to human health. Emerging evidence suggests that probiotics are a safer substitute, although little research has explored this in Bangladesh. We recently isolated local bacterial strains with probiotic properties. We aimed to determine their impact on the growth, hematobiochemical parameters, and production costs of broiler chicks relative to that of a commercial probiotic (CP) and AGP.

Materials and Methods: Day-old male broiler chicks (Cobb 500, n=63) were divided equally into three experimental groups (three replicates per group and seven chicks per replicate). First group was fed a basal diet supplemented with the AGP, ciprofloxacin (CTL group), second group was fed a basal diet supplemented with the CP, Protexin® (CP group), and the third group was fed a basal diet supplemented with our isolated bacterial strains (study probiotic [SP] group) for 36 days. Body weight was recorded daily, and relative growth rate (RGR), feed conversion ratio (FCR), and organ weights and carcass yields were calculated at the study's end. Blood obtained on day 36 was used to determine the number of red blood cell (RBC) and white blood cells (WBCs), hemoglobin (Hb) concentration, packed cell volume, erythrocyte sedimentation rate, as well as levels of serum glucose, total and high-density lipoprotein (HDL) cholesterol, and triglycerides (TGs). Total production costs were estimated by summing the variable and fixed costs.

Results: Chicks in both the CP and SP groups experienced significant decreases in blood glucose levels and significant increases in BW, RGR, FCR, levels of RBC and WBC, Hb concentration, and packed cell volume compared with those in the CTL group (p<0.05 for all). Our data suggested a numerical reduction (p>0.05) in levels of total cholesterol, TGs, and HDL in the SP and CP groups when compared to the CTL group. In addition, both CP and SP treatments resulted in significant (p<0.05) gains in net profit compared with the treatment given to the CTL group.

Conclusion: Administration of probiotics, either from a commercialized or local source, led to greater improvements in growth, hematological parameters, and net profits of broiler chicks when compared with that of an AGP. This suggests that they are suitable alternatives to the AGPs used in poultry feed and that our isolated strains, in particular, are an ideal option for farmers in Bangladesh.

Keywords: Cobb 500 broiler, Enterococcus faeciumPediococcus acidilactici, probiotics, serum biochemistry.



Tuesday, 12 January 2021

Drinking water supplemented with wood vinegar on growth performance, intestinal morphology, and gut microbial of broiler chickens

Research (Published online: 12-01-2021)
12. Drinking water supplemented with wood vinegar on growth performance, intestinal morphology, and gut microbial of broiler chickens
Kornkamon Hanchai, Tassanee Trairatapiwan and Rachakris Lertpatarakomol
Veterinary World, 14(1): 92-96

ABSTRACT

Background and Aim: Wood vinegar is a product generated from the combustion and distillation of wood and other plant materials. It has been shown to suppress bacteria, resulting in healthier livestock and increased yields. This study aimed to determine the efficacy of drinking water supplemented with wood vinegar on growth performance, intestinal health, and gut microbial of broilers.

Materials and Methods: A total of 120 Ross 308 1-day-old male broiler chicks were randomly distributed in a completely randomized experimental design. The study consisted of three treatments containing four replicates, with 10 birds in each. Treatments were given 0.5% and 1.0% (V/V) wood vinegar supplemented in drinking water, while no supplementation was given to the control group. The animals were raised in an open-house system. All groups were provided with a commercial diet and drinking water ad libitum. Analysis of variance was conducted using the general linear model procedure to compare the levels of wood vinegar supplementation in drinking water on growth performance, intestinal morphology, and gut microbial.

Results: No significant differences (p>0.05) were found for body weight gain, feed intake, feed conversion ratio, and water consumption between groups during the starter (1-21 days old), grower (22-35 days old), and whole (1-35 days old) growth periods. Moreover, no significant differences in villi height and crypt depth (p>0.05) at 21 and 35 days of age were found. In addition, no significant difference in terms of lactic acid bacteria and Escherichia coli was found between the different treatments.

Conclusion: Drinking water supplemented with wood vinegar was not found to have an effect on the growth performance and gut microbial of broiler chickens in the present study. However, the supplementation of wood vinegar in drinking water could improve intestinal morphology.

Keywords: broiler, growth performance, gut microbial, intestinal morphology, wood vinegar.



Advanced molecular characterization of enteropathogenic Escherichia coli isolated from diarrheic camel neonates in Egypt

Research (Published online: 12-01-2021)
11. Advanced molecular characterization of enteropathogenic Escherichia coli isolated from diarrheic camel neonates in Egypt
Momtaz A. Shahein, Amany N. Dapgh, Essam Kamel, Samah F. Ali, Eman A. Khairy, Hussein A. Abuelhag and Ashraf S. Hakim
Veterinary World, 14(1): 85-91

ABSTRACT

Background and Aim: Camels are important livestock in Egypt on cultural and economic bases, but studies of etiological agents of camelid diseases are limited. The enteropathogen Escherichia coli is a cause of broad spectrum gastrointestinal infections among humans and animals, especially in developing countries. Severe infections can lead to death. The current study aimed to identify pathogenic E. coli strains that cause diarrhea in camel calves and characterize their virulence and drug resistance at a molecular level.

Materials and Methods: Seventy fecal samples were collected from diarrheic neonatal camel calves in Giza Governorate during 2018-2019. Samples were cultured on a selective medium for E. coli, and positive colonies were confirmed biochemically, serotyped, and tested for antibiotic susceptibility. E. coli isolates were further confirmed through detection of the housekeeping gene, yaiO, and examined for the presence of virulence genes; traT and fimH and for genes responsible for antibiotic resistance, ampC, aadB, and mphA. The isolates in the important isolated serotype, E. coli O26, were examined for toxigenic genes and sequenced.

Results: The bacteriological and biochemical examination identified 12 E. coli isolates from 70 fecal samples (17.1%). Serotyping of these isolates showed four types: O26, four isolates, 33.3%; O103, O111, three isolates each, 25%; and O45, two isolates, 16.7%. The isolates showed resistance to vancomycin (75%) and ampicillin (66.6%), but were highly susceptible to ciprofloxacin, norfloxacin, and tetracycline (100%). The structural gene, yaiO (115 bp), was amplified from all 12 E. coli isolates and traT and fimH genes were amplified from 10 and 8 isolates, respectively. Antibiotic resistance genes, ampC, mphA, and aadB, were harbored in 9 (75%), 8 (66.6%), and 5 (41.7%), respectively. Seven isolates (58.3%) were MDR. Real-time-polymerase chain reaction of the O26 isolates identified one isolate harboring vt1, two with vt2, and one isolate with neither gene. Sequencing of the isolates revealed similarities to E. coli O157 strains.

Conclusion: Camels and other livestock suffer various diseases, including diarrhea often caused by microbial pathogens. Enteropathogenic E. coli serotypes were isolated from diarrheic neonatal camel calves. These isolates exhibited virulence and multiple drug resistance genes.

Keywords: camel, multidrug-resistant Escherichia coli, real-time polymerase chain reaction, sequencing, virulence.



Monday, 11 January 2021

Effect of oxygen tension and antioxidants on the developmental competence of buffalo oocytes cultured in vitro

Research (Published online: 11-01-2021)
10. Effect of oxygen tension and antioxidants on the developmental competence of buffalo oocytes cultured in vitro
Amro M. El-Sanea, Ahmed Sabry S. Abdoon, Omaima M. Kandil, Nahed E. El-Toukhy, Amal M. Abo El-maaty and Hodallah H. Ahmed
Veterinary World, 14(1): 78-84

ABSTRACT

Aim: Oxidative stress (OS) is one of the major disruptors of oocyte developmental competence, which appears due to the imbalance between the production and neutralization of reactive oxygen species (ROS).

Materials and Methods: In Experiment 1, buffalo oocytes were in vitro matured, fertilized, and cultured at 38.5°C under 5% CO2 + 20% O2 in standard CO2 incubator (OS) or under 5% O2 + 5% CO2 + 90% N2 (Multi-gas incubator, low O2). In Experiment 2, buffalo cumulus oocytes complexes (COCs) were matured in Basic maturation medium (BMM) composed of TCM199+ 10% FCS+ 10 μg/ml FSH+ 50 μg/ml gentamicin (control group) or in BMM supplemented with 50 μM ascorbic acid (ascorbic acid group) or 3.0 mM glutathione (glutathione group) or 10-5 M melatonin (melatonin group) and cultured at 38.5°C under 20% O2 for 24 h. Matured buffalo oocytes in control, ascorbic acid, or melatonin groups were fertilized and zygotes were cultured for 8 days under the same conditions.

Results: In both experiments, maturation, cleavage, and blastocyst rates were recorded. Results showed that culture of buffalo oocytes under low O2 (5% O2) significantly increased maturation, cleavage, and blastocyst rates (p<0.05). Meanwhile, under 20% O2, addition of 10-5 M melatonin or 50 μM ascorbic acid to in vitro maturation (IVM) medium significantly improved cumulus cell expansion, nuclear maturation rates of buffalo oocytes (p<0.05), and increased cleavage and blastocyst rates (p<0.05).

Conclusion: About 5% O2 is the optimum condition for in vitro production of buffalo embryos, and addition of 10-5 M melatonin to IVM medium for oocytes cultured under 20% O2 could alleviate the adverse effect of high oxygen tension and increased embryo yield.

Keywords: antioxidants, buffalo oocytes, developmental competence, in vitro embryo production, O2 tension.



Comparison of two attenuated infectious bursal disease vaccine strains focused on safety and antibody response in commercial broilers

Research (Published online: 11-01-2021)
9. Comparison of two attenuated infectious bursal disease vaccine strains focused on safety and antibody response in commercial broilers
Thotsapol Thomrongsuwannakij, Nataya Charoenvisal and Niwat Chansiripornchai
Veterinary World, 14(1): 70-77

ABSTRACT

Background and Aim: Infectious bursal disease (IBD) or Gumboro disease is one of the most detrimental diseases in the poultry industry worldwide. Previous scientific studies have shown that live IBD vaccination might induce transient immunosuppression, leading to suboptimal vaccine responses, and therefore lack of protection against other infectious diseases; therefore, selecting an IBD vaccine in commercial farms is a concern. This study aims to compare two commercially attenuated IBD vaccines (intermediate and intermediate-plus strains) in terms of safety and antibody response to IBD and Newcastle disease viruses (NDV) in commercial broilers.

Materials and Methods: Overall, 216 Cobb broiler chickens were divided into three groups based on the IBD vaccine strain administered: V217 strain (Group 1), M.B. strain (Group 2), and an unvaccinated group (Group 3). Groups 1 and 2 were orally vaccinated with Hitchner B1 NDV vaccine strain 7 days after IBD vaccination. Blood samples were collected at IBD vaccination day (15 days of age) and at 7, 14, 21, and 28 days post-IBD vaccination. The immunosuppressive effects of the IBD vaccination were determined by NDV antibody response, the bursa:body weight (B:BW) ratio, and the histopathological lesion scores of the bursa of Fabricius. Phylogenetic analysis was also performed.

Results: Phylogenetic analysis revealed that the M.B. strain belonged to a very virulent IBD strain, whereas the V217 strain belonged to a classical IBD virus strain. NDV antibody titers of the two vaccinated groups increased after ND vaccination, reaching their maximum at 14 days post-ND vaccination and decreasing thereafter. The V217 group presented the highest NDV humoral response from 7 days post-vaccination (dpv) to the end of the study. The mean NDV antibody titer of the V217 group was significantly (p<0.05) higher than that of the M.B. group at 14 dpv. In addition, the V217 strain-induced lower bursal lesions post-IBD vaccination and a higher B:BW ratio at 7 and 21 dpv compared to the M.B. group. The higher B:BW ratio, lower bursal lesions, and higher ND antibody response present in the V217 group indicate that the V217 strain induces lower immunosuppressive effects compared to the M.B. strain.

Conclusion: The results of this study indicate that IBD vaccine selection merits consideration, as avoiding the immunosuppressive effects induced by live IBD vaccination and the consequent impact on response to other vaccines is important.

Keywords: broilers, immunosuppressive effects, infectious bursal disease, vaccination.



Saturday, 9 January 2021

Changes in hematological, biochemical, and blood gases parameters in response to progressive inclusion of nitrate in the diet of Holstein calves

Research (Published online: 09-01-2021)
8. Changes in hematological, biochemical, and blood gases parameters in response to progressive inclusion of nitrate in the diet of Holstein calves
Abimael Ortiz-Chura, Gisela Marcoppido, José Gere, Gustavo Depetris, Francisco Stefañuk, Marcos D. Trangoni, Silvio L. Cravero, Claudia Faverín, Angel Cataldi and María E. Cerón-Cucchi
Veterinary World, 14(1): 61-69

ABSTRACT

Background and Aim: Nitrate (NO3) reduces enteric methane emissions and could be a source of non-protein nitrogen in ruminant feeds. Nonetheless, it has a potential toxic effect that could compromise animal health and production. The purpose of this study was to determine the effects of progressive inclusion of NO3 in the diet on the hematological, biochemical, and blood gases parameters, in turn, the effects on feed intake and live weight gain (LWG) in Holstein calves.

Materials and Methods: Eighteen Holstein heifers and steers (nine animals/treatment) were maintained in individual pens for 45 days. Animals were randomly allocated to either a control or nitrate diet (ND) (containing 15 g of NO3/kg of dry matter [DM]). The biochemical parameters and blood gases were analyzed only in the NO3 group on days: -1, 1, 7, 13, 19, and 25 corresponding to 0, 20, 40, 60, 80, and 100% of the total inclusion of NO3 in the diet, respectively. In addition, DM intake (DMI) and LWG were evaluated among dietary treatments.

Results: Feeding the ND did not influence DMI or LWG (p>0.05). Methemoglobin (MetHb) and deoxyhemoglobin increased according to the NO3 concentrations in the diet (p<0.05), while an opposite effect was observed for oxyhemoglobin and carboxyhemoglobin (p<0.05). Hematocrit levels decreased (p<0.05), while albumin, alanine aminotransferase, and gamma-glutamyl transpeptidase concentrations were not modified (p>0.05). However, glucose, urea, aspartate aminotransferase (AST), and retinol concentrations increased (p<0.05) according to the NO3 concentrations in the diet.

Conclusion: This study confirmed that the progressive inclusion of 123 g of NO3/animal/day in the diet could be safe without affecting DMI and LWG of Holstein calves. In turn, a dose-response effect of the MetHb, glucose, urea, AST, and retinol was observed, but these values did not exceed reference values. These results highlighted the importance of using a scheme of progressive inclusion of NO3 in the diet of calves to reduce the risks of NO3 toxicity.

Keywords: dry matter intake, liver function, methemoglobin, nitrate toxicity.