Evaluation of the bacteriocin produced by strain 9 lactic acid bacteria isolate for biopreservation

Research (Published online: 28-09-2020)

37. Evaluation of the bacteriocin produced by strain 9 lactic acid bacteria isolate for biopreservation

I Dewa Made Sukrama, Juliana Franciska and I Wayan Suardana

Veterinary World, 13(9): 2012-2019

ABSTRACT

Aim: This study aimed to determine the effect of the bacteriocin produced by strain 9 lactic acid bacteria (LAB) isolate on the biopreservation of beef.

Materials and Methods: The strain 9 LAB isolate was identified conventionally by culturing with de Man, Rogosa, and Sharpe broth medium followed by Gram staining and catalase testing. The molecular confirmation of the isolate involved analyzing the 16S rRNA gene with specific primers, that is, B27F (5-AGAGTTTGATCCTGGCTCAG-3) and U1492R (5-GGTTACCTTGTTACGACTT-3). Then, the isolate was centrifuged to evaluate the bacteriocin production, and the effect of the biopreservative activity in beef was evaluated by measuring the NH3 produced with the Eber test and the organoleptic acceptance from expert panels.

Results: This study confirmed that the strain 9 LAB isolate was a strain of Pediococcus pentosaceus, and the bacteriocin product showed biopreservative potential. The biopreservative potential was characterized by a significant decline in the production of NH3 and the panel's acceptance of the texture and tenderness of the beef, compared with the control, after 10 days of constant treatment.

Conclusion: This study highlighted the high biopreservative potency of pediocin produced by P. pentosaceus strain 9. This was noted by the production of NH3 and the modifications in texture and tenderness.

Keywords: bacteriocin, beef, biopreservative, lactic acid bacteria.

Influence of pre and postpartum alpha-tocopherol supplementation on milk yield, milk quality, and udder health of Jersey crossbred cows at tropical lower Gangetic region

Research (Published online: 26-09-2020)

36. Influence of pre and postpartum alpha-tocopherol supplementation on milk yield, milk quality, and udder health of Jersey crossbred cows at tropical lower Gangetic region

Amit Kumar Singh, Champak Bhakat, Tripti Kumari, Dilip Kumar Mandal, Anupam Chatterjee, Muthupalani Karunakaran and Tapas Kumar Dutta

Veterinary World, 13(9): 2006-2011

ABSTRACT

Background and Aim: Alpha-tocopherol supplementation influences milk yield, milk quality, and udder health of dairy cows, which needs to be investigated for Jersey crossbred cows at hot-humid climate. Therefore, the present study was framed with an objective to study the effect of pre and postpartum Alpha-tocopherol supplementation on milk yield, milk quality, and udder health status of Jersey crossbred cows at tropical climate.

Materials and Methods: For this study, 19 similar parity, body condition score, and production level dairy animals were separated randomly into three groups, namely, Control, T1 and T2. Control group (no supplementation) was compared with two treatment groups, namely, T1 and T2. Supplementation of Alpha-tocopherol was done in concentrate fed to the animals (at 1 g/cow/day) 30 days prepartum to 30 days postpartum in T1 and 30 days prepartum to 60 days postpartum in T2 groups. Observations were taken for different parameters up to 5 months of lactation.

Results: Statistically analyzed data revealed that overall significantly (p<0.01) more milk production was found in T2, followed by T1 than the control group. Overall significantly (p<0.01) lower somatic cell counts and modified California mastitis tests were recorded in T2, followed by T1 than the control group. Overall significantly (p<0.01), better milk quality in terms of methylene blue reduction test was found in T2, followed by T1 than control groups. Differences in the milk composition of all three groups were non-significant (p>0.05).

Conclusion: Supplementation of Alpha-tocopherol during prepartum to initial lactation period may enhance milk yield, milk quality, and udder health status of Jersey crossbred cows at the tropical lower Gangetic region.

Keywords: alpha-tocopherol, hot-humid region, jersey crossbred, milk production, udder health.

The COVID-19: Current understanding

Review (Published online: 26-09-2020)

35. The COVID-19: Current understanding

Shweta Tripathi and Mayukh Mani Tripathi

Veterinary World, 13(9): 1998-2005

ABSTRACT

In December 2019, China reported several cases of a new coronavirus disease (COVID-19). The COVID-19 outbreak, which was initially limited to Wuhan, China, has rapidly spread worldwide. Infection of the disease occurs through exposure to the virus through inhalation of respiratory droplets or if a person touches a mucosal surface after touching an object with the virus on it. The common symptoms of COVID-19 are fever, dry cough, dyspnea (difficult or labored breathing), fatigue, chest pain, and myalgia (muscle pain), etc. Real-time polymerase chain reaction is used to detect the virus in sputum, throat, nasal swabs, and secretion of lower respiratory samples. Early diagnosis, isolation, and supportive care are necessary for the treatment of the patients. The present review aims to provide recent information on COVID-19 related to its epidemiology, clinical symptoms, and management. This article also summarizes the current understanding of severe acute respiratory syndrome coronavirus-2 and its history of origin.

Keywords: coronavirus disease 2019, diagnosis, severe acute respiratory syndrome coronavirus-2, treatments.

Serological study on brucellosis in captive elephants (Elephas maximus) and stray dogs in North Thailand

Research (Published online: 26-09-2020)

34. Serological study on brucellosis in captive elephants (Elephas maximus) and stray dogs in North Thailand

Suchanit Ngamkala, Taweepoke Angkawanish, Weerapun Nokkaew and Nikorn Thongtip

Veterinary World, 13(9): 1992-1997

ABSTRACT

Background and Aim: Brucellosis is considered as an important zoonotic disease caused by various strains of Brucella in numerous host species. Although brucellosis has been reported in almost animal species, the relevance of brucellosis infection and diagnostic technique in Asian elephant (Elephas maximus) has been limited. The present serological investigation aimed to investigate the antibody response to Brucella abortus in captive Asian elephants in North Thailand. Moreover, further serological survey was also conducted to detect the antibody response to Brucella canis in stray dogs cohabiting the same area as the elephant herd.

Materials and Methods: Serum samples were collected from 40 captive Asian elephants and submitted for serological analysis based on B. abortus antigen using Rose Bengal plate test (RBPT) in combination with ethylenediaminetetraacetic acid-tube agglutination test (EDTA-TAT) as a supplementary test and by commercial indirect enzyme-linked immunosorbent assay (iELISA). In addition, serum samples were also obtained from 16 stray dogs that live nearby the elephant-raising area and were tested using commercial Dot-ELISA based on B. canis antigen.

Results: Serological analysis in captive Asian elephants showed 100% seronegative (40/40) from all serological tests response to B. abortus. For stray dogs, 12.5% (2/16) had a low positive reaction response to B. canis.

Conclusion: The serological survey for brucellosis in Asian elephant was adapted and applied using RBPT, EDTA-TAT, and iELISA in the present study. For future evaluation, we recommended the use of a combination of serological tests with validation together with comparing by direct detection such as bacterial isolation to provide an appropriate brucellosis surveillance program in Asian elephants. In addition, the surveillance of stray dogs or multispecies habitation should be kept into considerations.

Keywords: Asian elephants, brucellosis, serology, stray dogs.

Contamination factors associated with surviving bacteria in Thai commercial raw pet foods

Research (Published online: 25-09-2020)

33. Contamination factors associated with surviving bacteria in Thai commercial raw pet foods

Suppada Kananub, Nayika Pinniam, Sitthiporn Phothitheerabut and Praphaphan Krajanglikit

Veterinary World, 13(9): 1988-1991

ABSTRACT

Aim: This study aimed to identify the surviving bacteria in commercial raw pet food and to analyze the factors associated with their contamination.

Materials and Methods: A total of 17 samples from 12 brands available in Thailand were randomly selected for analysis. Fifteen samples were frozen products and two were freeze-dried. The total bacterial counts (TBCs) of Clostridium perfringens, Campylobacter spp., Staphylococcus aureus, Escherichia coli, Salmonella spp., Listeria spp., and Listeria monocytogenes were measured. Association between the bacterial profile and feed ingredients, as well as with product types, was analyzed by Chi-squared and Fisher's exact tests.

Results: Campylobacter was not found in any product, whereas Salmonella spp. and Listeria spp. showed the highest prevalence with respect to the standard's limits. The TBC was significantly related to the type of the products (frozen or freeze-dried), and S. aureus and L. monocytogenes were significantly related to a chicken-based diet.

Conclusion: Pet food contamination can occur during the manufacturing process, storage, or even preparation. The freezing and drying processes may reduce, but not eradicate, the bacterial contamination in raw pet food. These results emphasize the need for quality control in the manufacturing process and show the importance of personal hygiene for the pet owner to reduce health risks.

Keywords: foodborne pathogen, freeze-dried product frozen product, meat types, raw pet food.

Productivity, absence of a bull and endoparasitic nematodiosis in beef cattle farms in an upland area of East Java, Indonesia

Research (Published online: 25-09-2020)

32. Productivity, absence of a bull and endoparasitic nematodiosis in beef cattle farms in an upland area of East Java, Indonesia

Widi Nugroho, Siska Aditya, Rahadi Swastomo and Aulanni'am Aulanni'am

Veterinary World, 13(9): 1982-1987

ABSTRACT

Background and Aim: Cattle are an important economic asset for the rural community in East Java Province, Indonesia. The study aimed to provide updated data of cattle farm demography, productivity, as well as the role of the absence of a bull and nematodiosis in reduced productivity of beef cattle in an upland rural area of the province.

Materials and Methods: The study was conducted in Sukowono village, Bondowoso region. A Census survey was conducted to collect data through interviews with farmers. Further, 102 fecal samples were taken systematically and processed using a double centrifugation method to investigate the endoparasitic nematodiosis in the cattle population. The demographic data, productivity, and nematodiosis were analyzed descriptively. The difference between proportions was analyzed using Chi-square with 95% confidence limit. The associations were described in risk ratio with 95% confidence interval (CI).

Results: The total cattle population was 814 heads; the range of farm size was 1-7 (median: 2) cattle. Female cattle comprised 81.8% (666/814) of the cattle population but, only 5.5% (23/422) farmers kept both bull and mature female cattle. Pregnancy rate was 26.8% (145/542) of mature female cattle. The delayed first calving time appeared in 24.8% (62/250) of heifers and calving interval of >14 months occurred in 83.2% (149/179) of multiparous cows. The prevalence of endoparasitic nematodiosis was 43.1% (44/102, 95%, CI: 38.1-52.1%). Either the absence of the bull or the nematodiosis did not associate with pregnancy rate or calving interval of cows.

Conclusion: This study indicates that the productivity of the cattle in the study area was low but may not associate with the absence of a bull or nematodiosis.

Keywords: beef cattle farm, bull, nematodiosis, productivity.

Effects of respiratory disease on Kele piglets lung microbiome, assessed through 16S rRNA sequencing

Research (Published online: 25-09-2020)

31. Effects of respiratory disease on Kele piglets lung microbiome, assessed through 16S rRNA sequencing

Jing Zhang, Kaizhi Shi, Jing Wang, Xiong Zhang, Chunping Zhao, Chunlin Du and Linxin Zhang

Veterinary World, 13(9): 1970-1981

ABSTRACT

Background and Aim: Due to the incomplete development of the immune system in immature piglets, the respiratory tract is susceptible to invasion by numerous pathogens that cause a range of potential respiratory diseases. However, few studies have reported the changes in pig lung microorganisms during respiratory infection. Therefore, we aimed to explore the differences in lung environmental microorganisms between healthy piglets and piglets with respiratory diseases.

Materials and Methods: Histopathological changes in lung sections were observed in both diseased and healthy pigs. Changes in the composition and abundance of microbiomes in alveolar lavage fluid from eleven 4-week-old Chinese Kele piglets (three clinically healthy and eight diseased) were studied by IonS5TM XL sequencing of the bacterial 16S rRNA genes.

Results: Histopathological sections showed that diseased pigs displayed more lung lesions than healthy pigs. Diseased piglets harbored lower bacterial operational taxonomic units, α-diversity, and bacterial community complexity in comparison to healthy piglets. Taxonomic composition analysis showed that in the diseased piglets, the majority of flora was composed of Ureaplasma, Mycoplasma, and Actinobacillus; while Actinobacillus, Sphingomonas, and Stenotrophomonas were dominant in the control group. The abundance of Ureaplasma was significantly higher in ill piglets (p<0.05), and the phylogenetic tree indicated that Ureaplasma was clustered in Ureaplasma diversum, a conditional pathogen that has the potential to affect the swine respiratory system.

Conclusion: The results of this study show that the microbial species and structure of piglets' lungs were changed during respiratory tract infection. The finding of Ureaplasma suggested that besides known pathogens such as Mycoplasma and Actinobacillus, unknown pathogens can exist in the respiratory system of diseased pigs and provide a potential basis for clinical treatment.

Keywords: 16S rRNA sequencing, Kele piglets, microbial diversity, respiratory diseases, Ureaplasma, Ureaplasma diversum.