Identification and differentiation of Campylobacter isolated from chicken meat using real-time polymerase chain reaction and high resolution melting analysis of hipO and glyA genes

Research (Published online: 15-09-2020)

18. Identification and differentiation of Campylobacter isolated from chicken meat using real-time polymerase chain reaction and high resolution melting analysis of hipO and glyA genes

Ika Kartika Syarifah, Hadri Latif, Chaerul Basri and Puji Rahayu

Veterinary World, 13(9): 1875-1883

ABSTRACT

Background and Aim: Campylobacter species have been recognized as the most frequently identified bacterial cause of human gastroenteritis. The aims of this study were to identify Campylobacter jejuni and Campylobacter coli species isolated from chicken meat and to analyze the differences in the melting curve patterns of both species.

Materials and Methods: A total of 105 chicken meat samples collected from slaughterhouses and retailers in six provinces in Indonesia were examined for the isolation and identification of Campylobacter spp. A total of 56 positive isolates of Campylobacter spp. were analyzed using the quantitative real-time polymerase chain reaction and high resolution melting method.

Results: The prevalence of Campylobacter spp. in chicken meat was found to be 61.9%. Regarding the identification, 23 isolates (41.07%) were C. jejuni, 22 (39.29%) were C. coli, six (10.71%) were a mix between C. jejuni and C. coli, and five isolates (8.93%) were Campylobacter spp. All the C. jejuni and C. coli isolates produced varied melting curve patterns.

Conclusion: The high prevalence of C. jejuni and C. coli in chicken meat in Indonesia indicates a high risk of the incidence of campylobacteriosis in humans.

Keywords: Campylobacter coli, Campylobacter jejuni, chicken meat, glyA, hipO, melting curves.

Chronic unpredictable stress-induced inflammation and quantitative analysis of neurons of distinct brain regions in Wistar rat model of comorbid depression

Research (Published online: 15-09-2020)

17. Chronic unpredictable stress-induced inflammation and quantitative analysis of neurons of distinct brain regions in Wistar rat model of comorbid depression

Vandana Blossom, Megha Gokul, Nayanatara Arun Kumar, Rekha D. Kini, Shyamala Nayak and K. Bhagyalakshmi

Veterinary World, 13(9): 1870-1874

ABSTRACT

Background and Aim: Depression and anxiety are the most prominent neuropsychiatric disease and have been considered as the most burdensome diseases of society. The hippocampus and prefrontal cortex have a prominent role in stress-induced neurological disorders. Chronic unpredictable stress exposed rats are a perfect model in understanding comorbid depression and anxiety disorders. The inflammatory response occurring in the body has been linked to C-reactive protein (CRP) in many diseased conditions. The present research primarily focus on the possible correlation of Cortisol, CRP level and neuronal assay in different regions of hippocampus, dentate gyrus (DG), and prefrontal cortex.

Materials and Methods: The control group of rats (n=6) was not exposed to any stress. Whereas, the experimental stress group (n=6) of rats was exposed to various stressors for 15 days. After the experimentation procedures, the blood samples were collected and brain dissection was done. The neurons in the prefrontal cortex, the DG along with various hippocampal regions was counted. Statistical analysis was performed using student's t-test and p<0.05 was expressed as statistically significant.

Results: Animals exposed to chronic unpredictable stressors showed a significant (p<0.0001) decrease in the neuronal count in prefrontal cortex and hippocampus. A significant rise in the serum cortisol (p<0.0001) and CRP (p<0.001) was witnessed in the stressed group.

Conclusion: Our results demonstrate that chronic unpredictable stress exposure has affected neurogenesis in prefrontal cortex and hippocampal regions. Decreased neurogenesis was well in coordinance with the increase in cortisol and CRP. The chronic unpredictable stress-induced inflammatory response correlated to various brain regions might provoke insights into a variety of new drugs targeting neurogenesis.

Keywords: anxiety, C reactive protein, cortisol, depression, neuronal count, rat model, stress.

Effects of Sauropus androgynus extract and its combination with ampicillin against Methicillin-resistant Staphylococcus aureus: An in vitro study

Research (Published online: 14-09-2020)

6. Effects of Sauropus androgynus extract and its combination with ampicillin against Methicillin-resistant Staphylococcus aureus: An in vitro study

Asih Rahayu, Chylen Setiyo Rini, Yos Adi Prakoso, Bagus Uda Palgunadi and Muhammad Aris Munandar

International Journal of One Health, 6(2): 128-133

ABSTRACT

Background and Aim: The massive utilization of antibiotics has increased resistant genes produced by bacteria. Many bacteria, including Methicillin-resistant Staphylococcus aureus (MRSA), have become resistant against ampicillin (AMP). The combination of an herbal extract with AMP is expected to generate synergistic effects and may restore the susceptibility of MRSA against AMP. This study aimed to analyze the potency of Sauropus androgynous extract (SAE) as a single extract and combination with AMP against MRSA.

Materials and Methods: Sauropus androgynous was extracted using 60% ethanol. SAE biochemical compounds were analyzed qualitatively and quantitatively. SAE, AMP, and SAE+AMP were tested against MRSA isolates to determine the minimum inhibitory concentration and fractional inhibitory concentration. The inhibition of penicillin-binding proteins 2a (PBP2a) was analyzed using a latex agglutination test. Further, the disruptive membrane effects of SAE, AMP, and SAE+AMP were analyzed using a scanning electron microscope. The analysis of data was conducted using SPSS version 16 with p=0.01.

Results: SAE contained bioactive compounds such as phenolics and flavonoids. Further, 2 mg/mL of SAE could be used as the potential concentration against MRSA isolates in vitro. In addition, the utilization of SAE+AMP generated synergistic effects, restored the susceptibility of isolates against AMP, decreased the synthesis of PBP2a by the MRSA, and induced ultrastructural changes in the bacterial membrane.

Conclusion: This study indicated that the utilization of SAE potentially inhibits the growth of MRSA through decreasing of PBP2a expression, disruption of the MRSA membrane, while the combination of SAE+AMP showed synergistic effects against MRSA.

Keywords: ampicillin, herbal extract, Methicillin-resistant Staphylococcus aureus, Sauropus androgynus, ultrastructure.

Leukocyte populations and their cell adhesion molecules expression in newborn dromedary camel calves

Research (Published online: 12-09-2020)

16. Leukocyte populations and their cell adhesion molecules expression in newborn dromedary camel calves

Muaadh M. Gaashan, Abdullah I. A. Al-Mubarak and Jamal Hussen

Veterinary World, 13(9): 1863-1869

ABSTRACT

Background and Aim: Different properties of the newborn immune system have been characterized in many species. For the newborn camel calf, however, the phenotype and composition of blood leukocytes have so far not been evaluated. The current study aimed to analyze the distribution of leukocyte subpopulations and their expression pattern of cell adhesion molecules in newborn and adult dromedary camels.

Materials and Methods: Blood samples were collected from 17 newborn camel calves and 32 adult camels. For each sample, total leukocytes were separated and analyzed for their composition and cell adhesion molecules expression by flow cytometry.

Results: In comparison to adult camels, newborn camel calves had higher leukocyte numbers and higher numbers of neutrophils, monocytes, and lymphocytes but lower numbers of eosinophils in their blood. Among the lymphocyte populations in calves, the fractions of B cells and γδ T cells were elevated when compared to adults, whereas CD4-positive T cells were reduced. The comparison between camel calves and adult camels revealed significantly lower expression of the cell adhesion molecules CD11a, CD11b, and CD18 on granulocytes, monocytes, and lymphocytes in calves.

Conclusion: Newborn camel calves show a distinct composition and phenotype pattern of blood leukocytes when compared to adult camels. The observed rise in many leukocyte populations in calf blood may be due to reduced migratory activity in calf leukocyte populations.

Keywords: adhesion molecules, flow cytometry, immunophenotype, leukocytes, newborn camel calf.

Individual and combined anti-trypanosomal effects of arteether and diminazene aceturate in the treatment of experimental Trypanosoma brucei brucei infection in rats

Research (Published online: 11-09-2020)

15. Individual and combined anti-trypanosomal effects of arteether and diminazene aceturate in the treatment of experimental Trypanosoma brucei brucei infection in rats

Tobias Nnia Egbe-Nwiyi, Samson Eneojo Abalaka, Nuhu Abdulazeez Sani, Oremeyi Zainab Tenuche and Idoko Sunday Idoko

Veterinary World, 13(9): 1858-1862

ABSTRACT

Aim: Trypanosomosis is a vital protozoan disease of man and animals with devastating consequences in the tropical parts of the world, necessitating the investigation of the effects of diminazene aceturate (DA) and arteether (AR) on Trypanosoma brucei brucei experimental infection in rats.

Materials and Methods: We used a total of 98 rats, which were divided into 14 groups (A-N) of seven rats each over 36 days after acclimatizing them. We administered 1×106 trypanosomes to the infected groups (B-N) with Group A as the unexposed control rats. Groups C-F became the infected and treated rats with 3.5 mg/kg, 7.0 mg/kg, 10.5 mg/kg, and 14.0 mg/kg of DA while Groups G-J became the infected and treated rats with 0.01 ml/kg, 0.02 ml/kg, 0.03 ml/kg, and 0.04 ml/kg of AR. Groups K-N became infected and treated rats with DA and AR combinations at similar doses.

Results: Parasitemia suppression occurred in Groups G-J only but became cleared in Groups C-F and K-N. Survival time varied significantly (p<0.05) between Group B and the other infected groups. We recorded anemia in all the infected rats while significant (p<0.05) splenomegaly and hepatomegaly occurred in Groups G-J only compared to the other groups.

Conclusion: AR did not inhibit or potentiate the anti-trypanosomal efficacy of DA, and therefore, it is comparatively less effective in combating T. brucei infection at the present doses and treatment regimen.

Keywords: albino rats, arteether, clinicopathology, diminazene aceturate, Trypanosoma brucei brucei.

Association of bovine uterine involution disturbances with serum neuropeptide concentrations

Research (Published online: 11-09-2020)

14. Association of bovine uterine involution disturbances with serum neuropeptide concentrations

Aida Abultdinova, Isatay Jakupov, Joachim Roth, Klaus Failing, Axel Wehrend and Marlene Sickinger

Veterinary World, 13(9): 1854-1857

ABSTRACT

Background and Aim: Puerperal diseases influence fertility and should be diagnosed as soon as possible. This study aimed to evaluate the applicability of serum concentrations of substance P (SP), vasoactive intestinal polypeptide (VIP), and interleukin (IL)1β in the early diagnosis of uterine involution disturbances.

Materials and Methods: Blood serum samples of 86 dairy cows from six different farms were harvested within the first 20 days after calving from cows with uterine involution disturbances and healthy controls, respectively. Serum concentrations for SP, VIP, and IL-1β were determined using commercially available ELISA test kits. Statistical analyses included timely changes in blood serum levels and group comparisons of healthy cows and cows with uterine disease.

Results: SP concentrations increased significantly within 20 days after calving (p<0.04) with no significant difference observed between the groups. Moreover, no significant differences were found between VIP and log IL-1β.

Conclusion: Results showed that none of the examined serum parameters seems suitable as indicator of uterine involution disorders. Due to the timely changes in serum concentrations of SP after calving, a correlation to diseases might not be precluded. Further research is needed as regards the establishment of normative values concerning this parameter.

Keywords: dairy cow, diagnostics, inflammation, neuropeptides, uterus involution.

Relative white blood cell counts, heterophil-to-lymphocyte ratio, and discovery of blood parasites in wild dugong (Dugong dugon) at Lingayan Island, Toli-toli, Indonesia

Research (Published online: 10-09-2020)

13. Relative white blood cell counts, heterophil-to-lymphocyte ratio, and discovery of blood parasites in wild dugong (Dugong dugon) at Lingayan Island, Toli-toli, Indonesia

Aryani Sismin Satyaningtijas, Agik Suprayogi, Huda Shalahudin Darusman, Ardiansyah Nurdin and Desrayni Hanadhita

Veterinary World, 13(9): 1849-1853

ABSTRACT

Aim: This study was conducted to investigate the relative white blood cell (WBC) counts and the heterophil-to-lymphocyte (H/L) ratio and to analyze the presence of blood parasites in wild dugongs at Lingayan Island. It is expected that the results of this study could provide additional knowledge about the physiological conditions of wild dugongs in their natural habitats, which can serve as basic data in dugong conservation efforts, especially in Indonesia.

Materials and Methods: A wild dugong was captured around Lingayan Island. Blood samples were collected from the saphenous vein, and blood smears were prepared immediately. The blood smears were examined for leukocyte identification, calculation of relative WBC counts, and presence of blood parasites. The H/L ratio was calculated based on the obtained relative WBC counts.

Results: The relative WBC counts included heterophils 19.4%, lymphocytes 76.4%, and monocytes 3.6%, and the H/L ratio was 0.25. Intraerythrocytic parasites were identified and suspected to be Anaplasma and Babesia.

Conclusion: This study reports leukocyte values from free-ranging dugongs captured in Lingayan Island, Indonesia. Based on the H/L ratio, the dugong examined, in this study, did not experience chronic stress. However, the discovery of blood parasites could be one of the threatening factors for the dugong population.

Keywords: Anaplasma, Babesia, blood smear, leukocytes, sirenian.