Leukocyte populations and their cell adhesion molecules expression in newborn dromedary camel calves

Research (Published online: 12-09-2020)

16. Leukocyte populations and their cell adhesion molecules expression in newborn dromedary camel calves

Muaadh M. Gaashan, Abdullah I. A. Al-Mubarak and Jamal Hussen

Veterinary World, 13(9): 1863-1869

ABSTRACT

Background and Aim: Different properties of the newborn immune system have been characterized in many species. For the newborn camel calf, however, the phenotype and composition of blood leukocytes have so far not been evaluated. The current study aimed to analyze the distribution of leukocyte subpopulations and their expression pattern of cell adhesion molecules in newborn and adult dromedary camels.

Materials and Methods: Blood samples were collected from 17 newborn camel calves and 32 adult camels. For each sample, total leukocytes were separated and analyzed for their composition and cell adhesion molecules expression by flow cytometry.

Results: In comparison to adult camels, newborn camel calves had higher leukocyte numbers and higher numbers of neutrophils, monocytes, and lymphocytes but lower numbers of eosinophils in their blood. Among the lymphocyte populations in calves, the fractions of B cells and γδ T cells were elevated when compared to adults, whereas CD4-positive T cells were reduced. The comparison between camel calves and adult camels revealed significantly lower expression of the cell adhesion molecules CD11a, CD11b, and CD18 on granulocytes, monocytes, and lymphocytes in calves.

Conclusion: Newborn camel calves show a distinct composition and phenotype pattern of blood leukocytes when compared to adult camels. The observed rise in many leukocyte populations in calf blood may be due to reduced migratory activity in calf leukocyte populations.

Keywords: adhesion molecules, flow cytometry, immunophenotype, leukocytes, newborn camel calf.

Individual and combined anti-trypanosomal effects of arteether and diminazene aceturate in the treatment of experimental Trypanosoma brucei brucei infection in rats

Research (Published online: 11-09-2020)

15. Individual and combined anti-trypanosomal effects of arteether and diminazene aceturate in the treatment of experimental Trypanosoma brucei brucei infection in rats

Tobias Nnia Egbe-Nwiyi, Samson Eneojo Abalaka, Nuhu Abdulazeez Sani, Oremeyi Zainab Tenuche and Idoko Sunday Idoko

Veterinary World, 13(9): 1858-1862

ABSTRACT

Aim: Trypanosomosis is a vital protozoan disease of man and animals with devastating consequences in the tropical parts of the world, necessitating the investigation of the effects of diminazene aceturate (DA) and arteether (AR) on Trypanosoma brucei brucei experimental infection in rats.

Materials and Methods: We used a total of 98 rats, which were divided into 14 groups (A-N) of seven rats each over 36 days after acclimatizing them. We administered 1×106 trypanosomes to the infected groups (B-N) with Group A as the unexposed control rats. Groups C-F became the infected and treated rats with 3.5 mg/kg, 7.0 mg/kg, 10.5 mg/kg, and 14.0 mg/kg of DA while Groups G-J became the infected and treated rats with 0.01 ml/kg, 0.02 ml/kg, 0.03 ml/kg, and 0.04 ml/kg of AR. Groups K-N became infected and treated rats with DA and AR combinations at similar doses.

Results: Parasitemia suppression occurred in Groups G-J only but became cleared in Groups C-F and K-N. Survival time varied significantly (p<0.05) between Group B and the other infected groups. We recorded anemia in all the infected rats while significant (p<0.05) splenomegaly and hepatomegaly occurred in Groups G-J only compared to the other groups.

Conclusion: AR did not inhibit or potentiate the anti-trypanosomal efficacy of DA, and therefore, it is comparatively less effective in combating T. brucei infection at the present doses and treatment regimen.

Keywords: albino rats, arteether, clinicopathology, diminazene aceturate, Trypanosoma brucei brucei.

Association of bovine uterine involution disturbances with serum neuropeptide concentrations

Research (Published online: 11-09-2020)

14. Association of bovine uterine involution disturbances with serum neuropeptide concentrations

Aida Abultdinova, Isatay Jakupov, Joachim Roth, Klaus Failing, Axel Wehrend and Marlene Sickinger

Veterinary World, 13(9): 1854-1857

ABSTRACT

Background and Aim: Puerperal diseases influence fertility and should be diagnosed as soon as possible. This study aimed to evaluate the applicability of serum concentrations of substance P (SP), vasoactive intestinal polypeptide (VIP), and interleukin (IL)1β in the early diagnosis of uterine involution disturbances.

Materials and Methods: Blood serum samples of 86 dairy cows from six different farms were harvested within the first 20 days after calving from cows with uterine involution disturbances and healthy controls, respectively. Serum concentrations for SP, VIP, and IL-1β were determined using commercially available ELISA test kits. Statistical analyses included timely changes in blood serum levels and group comparisons of healthy cows and cows with uterine disease.

Results: SP concentrations increased significantly within 20 days after calving (p<0.04) with no significant difference observed between the groups. Moreover, no significant differences were found between VIP and log IL-1β.

Conclusion: Results showed that none of the examined serum parameters seems suitable as indicator of uterine involution disorders. Due to the timely changes in serum concentrations of SP after calving, a correlation to diseases might not be precluded. Further research is needed as regards the establishment of normative values concerning this parameter.

Keywords: dairy cow, diagnostics, inflammation, neuropeptides, uterus involution.

Relative white blood cell counts, heterophil-to-lymphocyte ratio, and discovery of blood parasites in wild dugong (Dugong dugon) at Lingayan Island, Toli-toli, Indonesia

Research (Published online: 10-09-2020)

13. Relative white blood cell counts, heterophil-to-lymphocyte ratio, and discovery of blood parasites in wild dugong (Dugong dugon) at Lingayan Island, Toli-toli, Indonesia

Aryani Sismin Satyaningtijas, Agik Suprayogi, Huda Shalahudin Darusman, Ardiansyah Nurdin and Desrayni Hanadhita

Veterinary World, 13(9): 1849-1853

ABSTRACT

Aim: This study was conducted to investigate the relative white blood cell (WBC) counts and the heterophil-to-lymphocyte (H/L) ratio and to analyze the presence of blood parasites in wild dugongs at Lingayan Island. It is expected that the results of this study could provide additional knowledge about the physiological conditions of wild dugongs in their natural habitats, which can serve as basic data in dugong conservation efforts, especially in Indonesia.

Materials and Methods: A wild dugong was captured around Lingayan Island. Blood samples were collected from the saphenous vein, and blood smears were prepared immediately. The blood smears were examined for leukocyte identification, calculation of relative WBC counts, and presence of blood parasites. The H/L ratio was calculated based on the obtained relative WBC counts.

Results: The relative WBC counts included heterophils 19.4%, lymphocytes 76.4%, and monocytes 3.6%, and the H/L ratio was 0.25. Intraerythrocytic parasites were identified and suspected to be Anaplasma and Babesia.

Conclusion: This study reports leukocyte values from free-ranging dugongs captured in Lingayan Island, Indonesia. Based on the H/L ratio, the dugong examined, in this study, did not experience chronic stress. However, the discovery of blood parasites could be one of the threatening factors for the dugong population.

Keywords: Anaplasma, Babesia, blood smear, leukocytes, sirenian.

Interaction between stress hormones and phagocytic cells and its effect on the health status of dairy cows: A review

Review (Published online: 10-09-2020)

12. Interaction between stress hormones and phagocytic cells and its effect on the health status of dairy cows: A review

Mohanned Naif Alhussien and Ajay Kumar Dang

Veterinary World, 13(9): 1837-1848

ABSTRACT

Dairy cows are exposed to various stressors during their production cycle that makes them more susceptible to various diseases. Phagocytes (neutrophils and macrophages) are important soldiers of the innate immune system. Neutrophils are the first responders to an inflammatory response and stress and kill pathogens by generating reactive oxygen species and by the release of various antimicrobial peptides, enzymes, neutrophil extracellular trap formation, etc. Macrophages, the other phagocytes, are also the cleanup crew for the innate immune system that removes debris, pathogens, and dead neutrophils later on after an inflammatory response. The neuroendocrine system along with phagocytes exhibits an immunomodulatory potential during stressful conditions. Neuroendocrine system directly affects the activity of phagocytes by communicating bidirectionally through shared receptors and messenger molecules such as hormones, neurotransmitters, or cytokines. Different immune cells may show variable responses to each hormone. Short time exposure to stress can be beneficial, but repeated or extended exposure to stress may be detrimental to the overall health and well-being of an animal. Although some stresses associated with farming practices in dairy cows are unavoidable, better understanding of the interactions occurring between various stress hormones and phagocytic cells can help to reduce stress, improve productivity and animal welfare. This review highlights the role played by various stress hormones in modulating phagocytic cell performance of dairy cattle under inflammatory conditions.

Keywords: dairy cattle, inflammatory diseases, leukocyte trafficking, macrophages, neutrophils.

Mycobacterium tuberculosis complex in wildlife: Review of current applications of antemortem and postmortem diagnosis

Review (Published online: 09-09-2020)

11. Mycobacterium tuberculosis complex in wildlife: Review of current applications of antemortem and postmortem diagnosis

Yusuf Madaki Lekko, Peck Toung Ooi, Sharina Omar, Mazlina Mazlan, Siti Zubaidah Ramanoon, Sabri Jasni, Faez Firdaus Abdullah Jesse and Azlan Che-Amat

Veterinary World, 13(9): 1822-1836

ABSTRACT

Tuberculosis (TB) is a chronic inflammatory and zoonotic disease caused by Mycobacterium tuberculosis complex (MTBC) members, which affects various domestic animals, wildlife, and humans. Some wild animals serve as reservoir hosts in the transmission and epidemiology of the disease. Therefore, the monitoring and surveillance of both wild and domestic hosts are critical for prevention and control strategies. For TB diagnosis, the single intradermal tuberculin test or the single comparative intradermal tuberculin test, and the gamma-interferon test, which is regarded as an ancillary test, are used. Postmortem examination can identify granulomatous lesions compatible with a diagnosis of TB . In contrast, smears of the lesions can be stained for acid-fast bacilli, and samples of the affected organs can be subjected to histopathological analyses. Culture is the gold standard test for isolating mycobacterial bacilli because it has high sensitivity and specificity compared with other methods. Serology for antibody detection allows the testing of many samples simply, rapidly, and inexpensively, and the protocol can be standardized in different laboratories. Molecular biological analyses are also applicable to trace the epidemiology of the disease. In conclusion, reviewing the various techniques used in MTBC diagnosis can help establish guidelines for researchers when choosing a particular diagnostic method depending on the situation at hand, be it disease outbreaks in wildlife or for epidemiological studies. This is because a good understanding of various diagnostic techniques will aid in monitoring and managing emerging pandemic threats of infectious diseases from wildlife and also preventing the potential spread of zoonotic TB to livestock and humans. This review aimed to provide up-to-date information on different techniques used for diagnosing TB at the interfaces between wildlife, livestock, and humans.

Keywords: culture, ELISA, gamma interferon test, genotyping, histopathology, Mycobacterium tuberculosis complex, polymerase chain reaction, wildlife.

Molecular genotypes analysis of Cryptosporidium and Hymenolepis in rats on Lombok Island, Indonesia

Research (Published online: 08-09-2020)

5. Molecular genotypes analysis of Cryptosporidium and Hymenolepis in rats on Lombok Island, Indonesia

Ersandhi Resnhaleksmana, Wayan Tunas Artama, Mahardika Agus Wijayanti and Fihiruddin Fihiruddin

International Journal of One Health, 6(2): 123-127

ABSTRACT

Background and Aim: Cryptosporidium parvum and Hymenolepis nana are intestinal parasites that are commonly found in the unclean environment. Their presence in rats promotes the transmission of the cryptosporidiosis and hymenolepiasis to humans or animals nearby. This study aimed to determine the molecular characteristics of C. parvum and H. nana and their distribution in rats on Lombok Island.

Materials and Methods: C. parvum and H. nana were investigated in 50 rats from Lombok Island. The molecular-parasitological technique used was polymerase chain reaction and sequencing method.

Results: From 50 samples of rats' stool from 10 locations on Lombok Island, 8% (4/50) of C. parvum was detected molecularly with an 18S rRNA gene and 2% (1/50) of H. nana with COX 1 gene. Phylogenetic analysis indicated that C. parvum carrying rats on Lombok Island have a genetic relationship with C. parvum with Obi7 isolates, Japan and H. nana has a genetic relationship with Rodentolepis nana identified with Hn-VT isolates, India.

Conclusion: The highest incidence of parasites was found in rats that were caught in the urban areas of Lombok Island, West Nusa Tenggara, Indonesia. Immediately, public health programs in these types of contaminated areas should receive priority attention to prevent further transmission of the parasites from animals to human beings.

Keywords: Cryptosporidium, Hymenolepis, intestinal parasite, Rattus.