Mycobacterium tuberculosis complex in wildlife: Review of current applications of antemortem and postmortem diagnosis

Review (Published online: 09-09-2020)

11. Mycobacterium tuberculosis complex in wildlife: Review of current applications of antemortem and postmortem diagnosis

Yusuf Madaki Lekko, Peck Toung Ooi, Sharina Omar, Mazlina Mazlan, Siti Zubaidah Ramanoon, Sabri Jasni, Faez Firdaus Abdullah Jesse and Azlan Che-Amat

Veterinary World, 13(9): 1822-1836

ABSTRACT

Tuberculosis (TB) is a chronic inflammatory and zoonotic disease caused by Mycobacterium tuberculosis complex (MTBC) members, which affects various domestic animals, wildlife, and humans. Some wild animals serve as reservoir hosts in the transmission and epidemiology of the disease. Therefore, the monitoring and surveillance of both wild and domestic hosts are critical for prevention and control strategies. For TB diagnosis, the single intradermal tuberculin test or the single comparative intradermal tuberculin test, and the gamma-interferon test, which is regarded as an ancillary test, are used. Postmortem examination can identify granulomatous lesions compatible with a diagnosis of TB . In contrast, smears of the lesions can be stained for acid-fast bacilli, and samples of the affected organs can be subjected to histopathological analyses. Culture is the gold standard test for isolating mycobacterial bacilli because it has high sensitivity and specificity compared with other methods. Serology for antibody detection allows the testing of many samples simply, rapidly, and inexpensively, and the protocol can be standardized in different laboratories. Molecular biological analyses are also applicable to trace the epidemiology of the disease. In conclusion, reviewing the various techniques used in MTBC diagnosis can help establish guidelines for researchers when choosing a particular diagnostic method depending on the situation at hand, be it disease outbreaks in wildlife or for epidemiological studies. This is because a good understanding of various diagnostic techniques will aid in monitoring and managing emerging pandemic threats of infectious diseases from wildlife and also preventing the potential spread of zoonotic TB to livestock and humans. This review aimed to provide up-to-date information on different techniques used for diagnosing TB at the interfaces between wildlife, livestock, and humans.

Keywords: culture, ELISA, gamma interferon test, genotyping, histopathology, Mycobacterium tuberculosis complex, polymerase chain reaction, wildlife.

Molecular genotypes analysis of Cryptosporidium and Hymenolepis in rats on Lombok Island, Indonesia

Research (Published online: 08-09-2020)

5. Molecular genotypes analysis of Cryptosporidium and Hymenolepis in rats on Lombok Island, Indonesia

Ersandhi Resnhaleksmana, Wayan Tunas Artama, Mahardika Agus Wijayanti and Fihiruddin Fihiruddin

International Journal of One Health, 6(2): 123-127

ABSTRACT

Background and Aim: Cryptosporidium parvum and Hymenolepis nana are intestinal parasites that are commonly found in the unclean environment. Their presence in rats promotes the transmission of the cryptosporidiosis and hymenolepiasis to humans or animals nearby. This study aimed to determine the molecular characteristics of C. parvum and H. nana and their distribution in rats on Lombok Island.

Materials and Methods: C. parvum and H. nana were investigated in 50 rats from Lombok Island. The molecular-parasitological technique used was polymerase chain reaction and sequencing method.

Results: From 50 samples of rats' stool from 10 locations on Lombok Island, 8% (4/50) of C. parvum was detected molecularly with an 18S rRNA gene and 2% (1/50) of H. nana with COX 1 gene. Phylogenetic analysis indicated that C. parvum carrying rats on Lombok Island have a genetic relationship with C. parvum with Obi7 isolates, Japan and H. nana has a genetic relationship with Rodentolepis nana identified with Hn-VT isolates, India.

Conclusion: The highest incidence of parasites was found in rats that were caught in the urban areas of Lombok Island, West Nusa Tenggara, Indonesia. Immediately, public health programs in these types of contaminated areas should receive priority attention to prevent further transmission of the parasites from animals to human beings.

Keywords: Cryptosporidium, Hymenolepis, intestinal parasite, Rattus.

Improving endangered antelope habitat through water quality assessment and collaboration with Kenya Wildlife Service

Research (Published online: 08-09-2020)

4. Improving endangered antelope habitat through water quality assessment and collaboration with Kenya Wildlife Service

Tamie Joy Jovanelly

International Journal of One Health, 6(2): 118-122

ABSTRACT

Background and Aim: A population of endangered sitatunga antelope (Tragelaphus spekii) lives in a free-range environment at Impala Sanctuary in Kisumu, Kenya. Kenya Wildlife Service park officials suspected that increased demands on outdated sewage infrastructure caused animal drinking water sources to become contaminated which resulted in animal sickness and death. In this study, we complete a water quality assessment on open water sources within the park boundaries to determine if water was suitable for animal consumption.

Materials and Methods: For the assessment of water, we measure eight physical and chemical parameters (pH, temperature, fecal coliform, dissolved oxygen, biochemical oxygen demand, nitrates, total phosphates, and turbidity). These eight parameters were chosen because they are used to establish a water quality index (WQI) percentage which proved to be useful to communicate conditions to park rangers, stakeholders, and adjacent landowners.

Results: Through 6 months of assessments, data collection, and analysis, we determined that most open water sources are severely contaminated, ranking on the WQI from 46% to 58% (bad to medium). In addition, we compared our data to drinking water standards set by the U.S. Department of Agriculture for livestock to find that only two sites met the minimum criteria. The remaining four sites were exponentially contaminated with levels reaching 10× recommended values for animal health.

Conclusion: Following these findings, the park was able to attract money for sewage infrastructure rebuilds that resolved the contamination problems. Sickness and death of free-roaming animals, including the antelope, were reduced.

Keywords: animals and contaminated water, Kenya, water quality.

Hepatoprotective activity of okra (Abelmoschus esculentus L.) in sodium nitrite-induced hepatotoxicity

Research (Published online: 07-09-2020)

10. Hepatoprotective activity of okra (Abelmoschus esculentus L.) in sodium nitrite-induced hepatotoxicity

Sri Puji Astuti Wahyuningsih, Elma Sakinatus Sajidah, Baiq Naili Dewi Atika, Dwi Winarni and Manikya Pramudya

Veterinary World, 13(9): 1815-1821

ABSTRACT

Background and Aim: For years, people have used sodium nitrite as a food preservative. This study determined the effect of okra (Abelmoschus esculentus L.) pod methanol extract (OPME) on mice with hepatotoxicity induced by sodium nitrite. The flavonoid and total phenolic levels, serum biochemistry, and liver histology were examined.

Materials and Methods: Green okra pod extraction was performed using ethanol methanol solvent. Thirty adult male BALB/c mice (8-10 weeks, ∼30 g) were divided into six groups: Normal control, negative control (sodium nitrite 50 mg/kg BW exposure), and treatment groups (sodium nitrite exposure and OPME at doses of 50, 100, 200, and 400 mg/kg BW). Subsequently, they were exposed to sodium nitrite and administered multiple doses of OPME for 19 days by gavage. After that, serum was used for biochemical evaluation, and liver histological analysis was performed. All data were statistically analyzed (α=0.05).

Results: All doses of OPME reduced the levels of nitric oxide (NO), malondialdehyde (MDA), alanine aminotransferase (ALT), and aspartate aminotransferase (AST). In this research, both superoxide dismutase (SOD) and catalase (CAT) levels increased in all OPME-administered treatments . All doses also reduced necrotic cells, proportion of swollen cells, and inflammation in liver histological analysis. The results of this study showed that OPME exerted hepatoprotective effects by lowering MDA, NO, ALT, and AST levels. It also improved SOD and CAT levels and recovered damaged liver tissue to its normal state. The optimal dose of OPME was 50-100 mg/kg BW.

Conclusion: OPME has potential as a natural hepatoprotective agent against sodium nitrite exposure.

Keywords: hepatoprotective, liver histology, okra pods methanol extract, sodium nitrite.

Phylogenetic group determination and plasmid virulence gene profiles of colistin-resistant Escherichia coli originated from the broiler meat supply chain in Bogor, Indonesia

Research (Published online: 05-09-2020)

9. Phylogenetic group determination and plasmid virulence gene profiles of colistin-resistant Escherichia coli originated from the broiler meat supply chain in Bogor, Indonesia

Irma Rahayuningtyas, Agustin Indrawati, I Wayan Teguh Wibawan, Maria Fatima Palupi and Istiyaningsih Istiyaningsih

Veterinary World, 13(9): 1807-1814

ABSTRACT

Background and Aim: Pathogenic Escherichia coli contamination along the broiler meat supply chain is a serious public health concern. This bacterial infection with multidrug-resistant can lead to treatment failure. Several studies have revealed that avian pathogenic E. coli (APEC) and human extraintestinal pathogenic E. coli (ExPEC) showed a close genetic relationship and may share virulence genes. This study aimed to determine the phylogenetic group and virulence gene profiles in colistin-resistant E. coli obtained from the broiler meat supply chain in Bogor, West Java, Indonesia.

Materials and Methods: Fifty-eight archive isolates originated from the cloacal swab, litter, drinking water, inside plucker swab, fresh meat at small scale poultry slaughterhouses, and traditional markets were used in this study. All the isolates were characterized by a polymerase chain reaction to determine the phylogenetic group (A, B1, B2, or D) and virulence gene profiles with APEC marker genes (iutA, hlyF, iss, iroN, and ompT).

Results: Phylogenetic grouping revealed that the isolates belong to A group (34.48%), D group (34.48%), B1 group (17.24%), and B2 group (13.79%). The virulence gene prevalence was as follows: iutA (36%), hlyF (21%), ompT (21%), iroN (10%), and iss (9%). The B2 group presented with more virulence genes combinations. iroN, hlyF, and ompT genes were positively associated with the B2 group (p≤0.05).

Conclusion: Our results highlight the role of colistin-resistant E. coli originated from the broiler meat supply chain as a potential reservoir for human ExPEC virulence genes.

Keywords: broiler supply chain, Escherichia coli, phylogenetic group, virulence gene.

Anti-feline immunodeficiency virus reverse transcriptase properties of some medicinal and edible mushrooms

Research (Published online: 05-09-2020)

8. Anti-feline immunodeficiency virus reverse transcriptase properties of some medicinal and edible mushrooms

Supaphorn Seetaha, Siriluk Ratanabunyong, Lueacha Tabtimmai, Kiattawee Choowongkomon, Jatuporn Rattanasrisomporn and Khuanjarat Choengpanya

Veterinary World, 13(9): 1798-1806

ABSTRACT

Background and Aim: Feline immunodeficiency virus (FIV) causes AIDS-like symptoms in domestic and wild cats. Treatment of infected cats has been performed using human anti-HIV drugs, which showed some limitations. This study aimed to determine the anti-FIV potential of some mushrooms.

Materials and Methods: A total of 17 medicinal and edible mushrooms were screened to find their inhibitory effect against FIV reverse transcriptase (FIV-RT). Three solvents, water, ethanol, and hexane, were used to prepare crude mushroom extracts. Fluorescence spectroscopy was used to perform relative inhibition and 50% inhibitory concentrations (IC50) studies.

Results: The ethanol extract from dried fruiting bodies of Inonotus obliquus showed the strongest inhibition with an IC50 value of 0.80±0.16 μg/mL. The hexane extract from dried mycelium of I. obliquus and ethanol and water extracts from fresh fruit bodies of Phellinus igniarius also exhibited strong activities with the IC50 values of 1.22±0.20, 4.33±0.39, and 6.24±1.42 μg/mL, respectively. The ethanol extract from fresh fruiting bodies of Cordyceps sinensis, hexane extracts from dried mycelium of I. obliquus, ethanol extracts of Ganoderma lucidum, hexane extracts of fresh fruiting bodies of Morchella esculenta, and fresh fruiting bodies of C. sinensis showed moderate anti-FIV-RT activities with IC50 values of 29.73±12.39, 49.97±11.86, 65.37±14.14, 77.59±8.31, and 81.41±17.10 μg/mL, respectively. These mushroom extracts show anti-FIV potential.

Conclusion: The extracts from I. obliquus, P. igniarius, C. sinensis, and M. esculenta showed potential anti-FIV activity.

Keywords: feline immunodeficiency virus, fluorescence spectroscopy, mushrooms, crude extracts, reverse transcriptase.

Prophylactic impact of nano-selenium on performance, carcasses quality, and tissues' selenium concentration using reversed-phase high-performance liquid chromatography during microbial challenge in broiler chickens

Research (Published online: 04-09-2020)

7. Prophylactic impact of nano-selenium on performance, carcasses quality, and tissues' selenium concentration using reversed-phase high-performance liquid chromatography during microbial challenge in broiler chickens

Essam S. Soliman, Fadwa F. Mahmoud, Mai A. Fadel and Rania T. Hamad

Veterinary World, 13(9): 1780-1797

ABSTRACT

Background and Aim: Nano-selenium (NS) supplementation contributes in improving productivity, performance, and meat quality while reducing public health concern. Influence of NS and inorganic selenium (Se) water additive on performance, carcass quality, immunoglobulin concentration, intestinal microbiota, Se tissue concentrations, and tissue architecture was studied.

Materials and Methods: Two-hundred and sixty 1-day-old Hubbard chicks were randomly grouped into five groups (5×52) and supplemented with 0.5 and 1.0 mL of NS and inorganic Se (100 mg.L-1). G1, G2, G3, and G4 were challenged with Escherichia coli O157: H7 2.6×108 on the 14th day. A total of 2250 samples, including 250 sera, 250 intestinal swabs, and 1500 organ and tissue samples as liver, spleen, heart, bursa, intestine, and breast muscles, and 250 eviscerated carcasses were collected.

Results: The results revealed a highly significant increase (p<0.01) in live body weights, weight gains, performance indices, carcasses, and organs weights, whereas immunoglobulin G and M concentrations in broilers treated with 0.5 and 1.0 mL NS, respectively, synchronized reveal a highly significant decline (p<0.01) in total bacterial and Enterobacteriaceae counts of intestinal swabs and breast muscles, final pH24, and drip loss in broilers treated with 0.5 and 1.0 mL NS, respectively. Meanwhile, water holding capacity revealed no significant differences between all groups. Reversed-phase high-performance liquid chromatography examination revealed the earlier disappearance of NS residues than inorganic Se from the broiler's liver and muscles. Histopathological photomicrographs of the liver, spleen, bursa of Fabricius, and intestine, as well as, the immunohistochemistry of intestinal sections revealed superior tissue architecture in broilers treated with NS contrary to inorganic Se.

Conclusion: The study showed significant stimulation actions of NS on performance, immunity, carcass and meat quality, intestinal and muscles' bacterial load as well as short withdrawal period and nearly normal cellular architecture compared to inorganic Se.

Keywords: broilers, carcass, immunity, nano-selenium, performance, reversed-phase high-performance liquid chromatography.