Protamine and other proteins in sperm and seminal plasma as molecular markers of bull fertility

Review (Published online: 25-03-2020)

24. Protamine and other proteins in sperm and seminal plasma as molecular markers of bull fertility

Berlin Pandapotan Pardede, Muhammad Agil and Iman Supriatna

Veterinary World, 13(3): 556-562

ABSTRACT

Fertility is the most important aspect in the efforts to increase livestock populations. Protamine and various proteins in sperm and seminal plasma are the results of the molecular analysis which can be used as a marker of fertility. Each of the proteins plays an important role in the normal function of sperm, starting from the formation of sperm structure, motility, capacitation, cell protection, acrosome reactions, successful fertilization, egg activation, and embryonic development. Finally, these molecular components can be a marker of fertility and can help to diagnose the cases of infertility/subfertility in livestock in the field.

Keywords: fertility, molecular marker, protamine, seminal plasma, sperm proteins.

The influence of electromagnetic radiation of cell phones on the behavior of animals

Research (Published online: 24-03-2020)

23. The influence of electromagnetic radiation of cell phones on the behavior of animals

Innar Sultangaliyeva, Raikhan Beisenova, Rumiya Tazitdinova, Akhan Abzhalelov and Marat Khanturin

Veterinary World, 13(3): 549-555

ABSTRACT

Background and Aim: The radiation emitted from cell phones has various deleterious effects on human health. The article considers the problem of the effects of electromagnetic radiation (EMR) of cell phones on the behavior of animals. The use of mobile phones is continually increasing throughout the world. All of the world population uses mobile phones widely; thus, having strong and daily effect on the brain. There is no restriction on the use of mobile phones. Getting more advantages and convenience of mobile communication, the population is trying not to hear the information about possible risks to their health, there is no element of self-restraint. The aim of this work was to study behavioral patterns in response to the influence of cell phones.

Materials and Methods: The experiments were carried out on 90 white outbred rats weighing 250-300 g. Experiments were carried out and the effect of EMR from Samsung Galaxy J1 mini and Xiaomi Redmi S2 phones on animal behavior was studied. Behavioral reactions were studied using the open field method.

Results: By the quality of the act of locomotion in the second experimental group is reduced by 30% and also in this group by the time of the act is 23% lower than in the control data. Vertical motor activity, a support stand, is also suppressed in the second experimental group: 61% lower in number and 47.2% lower in act time compared to the control group. Based on the data, we can conclude that the EMR of the phone Samsung Galaxy J1 Mini affects the behavior of animals to a greater extent than Xiaomi Redmi S2. By the frequency of the act, sniffing is 26% lower; by the time of the act, it remained at the level of control data in the second group. In the third group of the animals, the number and time of the act are 15% lower than the control data. The support stands as an indicator of research activity. In the second group the act of stand with support – by number was lower by 57% than the control data, this indicates that research activity is being suppressed.

Conclusion: According to the above changes under the action of EMR from the Samsung Galaxy J1 Mini and Xiaomi Redmi S2 phones, the motor component of behavior is suppressed. The research component of behavior in all groups of animals is enhanced due to high rates of sniffing. It is possible that in animals under stress, the motivation to interact with the environment decreases, leading to an excessive self-accentuation of the animal.

Keywords: cell phone, central nervous system, electromagnetic pollution, electromagnetic radiation, locomotion.

Molecular characterization of foot-and-mouth disease viruses collected from Northern and Central Ethiopia during the 2018 outbreak

Research (Published online: 24-03-2020)

22. Molecular characterization of foot-and-mouth disease viruses collected from Northern and Central Ethiopia during the 2018 outbreak

Yeneneh Tesfaye, Fazlurrahman Khan and Esayas Gelaye

Veterinary World, 13(3): 542-548

ABSTRACT

Background and Aim: Foot-and-mouth disease (FMD) is endemic in several developing countries and affects poor farmers through loss of production, death of diseased animals, and loss of animal byproducts. Forty-three samples were collected from 12 sites of five geographical located areas from suspected FMD virus (FMDV)-infected cattle during 2018. This study aimed to isolate and characterize the FMDVs using reverse transcription-polymerase chain reaction (RT-PCR) and gene sequencing.

Materials and Methods: Forty-three FMDV-suspected clinical samples cultured on BHK-21 cell were examined, followed by virus serotype identification using RT-PCR and gene sequencing.

Results: Twenty-nine (67.44%) samples were cultured on BHK-21 cell, of which 14 (32.56%) were not isolated; the 43 samples were analyzed using FMDV screening primers and serotype-specific primers. The contribution of the disease-causing serotype was serotype O of 8 (18.60%) samples, serotype A of 20 (46.51%) samples, and mixed infection (O and A) of 1 (2.33%) sample. Serotypes O and A were further characterized by phylogenetic analysis, which grouped them under East Africa 3 and Africa topotypes of genotype IV, respectively. Interestingly, serotype A was isolated for the 1st time from Keyet sub-woreda and Mulo woreda of Ethiopia, and mixed serotypes (O and A) were identified from the purchased animal.

Conclusion: Molecular test result, sequencing, and phylogenetic tree reconstruction analysis revealed that the 2018 FMD outbreak in Ethiopia was caused by FMDV serotypes O and A. FMDV serotype A was the predominant strain circulating in most study areas of the country. Infections in one sample with mixed serotypes of O and A were also reported. The authors recommend a vaccine matching study of those field isolated viruses with the vaccine strain.

Keywords: Ethiopia, foot-and-mouth disease virus serotypes, phylogenetic analysis, reverse transcription-polymerase chain reaction.

Veterinary World reviewer acknowledgment 2019

Reviewer Acknowledgment (Published online: 23-03-2020)

21. Veterinary World reviewer acknowledgment 2019

A. V. Sherasiya and Nazir

Veterinary World, 13(3): 538-541

The effect of crude guava leaf tannins on motility, viability, and intact plasma membrane of stored spermatozoa of Etawa crossbred goats

Research (Published online: 21-03-2020)

20. The effect of crude guava leaf tannins on motility, viability, and intact plasma membrane of stored spermatozoa of Etawa crossbred goats

Wurlina Wurlina, Mas'ud Hariadi, Erma Safitri, Suherni Susilowati and Dewa Ketut Meles

Veterinary World, 13(3): 530-537

ABSTRACT

Aim: The aim of this study was to know crude guava leaf tannins effect on motility, viability, and intact plasma membrane of stored spermatozoa of Etawa crossbred goats.

Materials and Methods: Macroscopic assessment of normal Etawa crossbred semen was followed by dilution with a glucose solution at a 1:10 ratio to increase volume. The diluted semen was treated by adding crude guava leaf tannins into 1 ml of the semen glucose diluent, and five treatments were obtained, namely, control group (C), with no added tannins; treatment Group 1 (T1), with 3%; treatment Group 2 (T2), with 6% tannins; treatment Group 3 (T3), with 12% tannins; and treatment Group 4 (T4), with 24% tannins. Each treatment used five replications. Then, microscopic analysis of the treated and control semen was carried out after 15 days of storage at 4-5°C temperature. The parameters observed were motility, pH, viability, abnormality, and intact spermatozoa plasma membrane.

Results: The spermatozoa motility in Group C was the highest (76.60±1.47). The motility in Group T1 did not differ from that in Group C, but was different and higher than that in Groups T2, T3, and T4. The pH of Group C tended to be acidic after 15 days of storage (4.78±0.01) as compared to the initial pH of fresh semen (6.76±0.12). The pH in Group C did not differ from that in the Groups T1 and T2, but differed from that in the T3 and T4 groups; the pH in the T3 and T4 groups was similar. The viability of spermatozoa in the T1 group was higher than that in all treatments (64.60±2.76); the lowest values were found in Group C (28.94±1.02). Group C had the lowest number of normal spermatozoa, with a mean of 72.58±3.48. The total number of abnormalities in the T2 group did not differ from those in the T3 group, and abnormalities in the T4 group did not differ from those in Group C, which exhibited the highest abnormalities in the head, neck, and tail. The most significant decrease was observed in the intact plasma membrane of spermatozoa on addition of 12% and 24% crude guava leaf tannin in glucose diluents.

Conclusion: The addition of 3% crude guava leaf tannin to crossbred Etawa goat semen diluted with glucose diluent and stored for 15 days at 4-5°C resulted in a significant effect on spermatozoa motility, viability, and intact plasma membrane, whereas the administration of 24% crude guava leaf tannin resulted in low live percentage of spermatozoa.

Keywords: crude tannins, intact plasma membrane, motility, spermatozoa, viability.

Diagnostic evaluation of a point-of-care test for culture and microbial susceptibility testing in canine dermatological infections in clinical practice

Research (Published online: 20-03-2020)

19. Diagnostic evaluation of a point-of-care test for culture and microbial susceptibility testing in canine dermatological infections in clinical practice

Roberta Perego, Eva Spada, Piera Anna Martino and Daniela Proverbio

Veterinary World, 13(3): 521-529

ABSTRACT

Background and Aim: Empirical antimicrobial therapy is frequently given in superficial bacterial folliculitis (SBF) and otitis externa (OE) in dogs, especially for the initial clinical presentation. Culture and subsequent antimicrobial susceptibility testing (AST) are generally limited to chronic cases with poor response to initial therapy. Several factors contribute to the failure to implement the use of AST in veterinary practice, i.e., long laboratory turnaround time or special requirements for sample shipping. Point-of-care (PoC) testing might reduce laboratory turnaround time and costs and the risk of emergence of multidrug-resistant pathogens. This study evaluated the Speed Biogram™ PoC test in canine SBF and OE compared with conventional methods for culture and AST.

Materials and Methods: Thirty-four canine samples were analyzed: eleven from SBF, seven from bacterial OE, four from mixed OE, six from Malassezia spp. OE, and six negative controls. Sensitivity (Se) and specificity (Sp) of the PoC test and the agreement between the PoC test and conventional methods were evaluated.

Results: Se and Sp of PoC test in discriminating between healthy and unhealthy subjects were 100% (95% confidence interval [CI] 87.66-100.00) and 100% (95% CI 54.1-100.0), respectively. For bacterial identification, the k value was 0.532. Se and Sp of PoC tests for AST were 81.73% (95% CI 72.95-88.63) and 93.10% (95% CI 88.86-96.98), respectively with a total good agreement between tests (mean k=0.714), but major (8/27) and very major (19/27) errors were observed in 55% of bacterial conventional culture-positive samples.

Conclusion: PoC test can identify dogs with SBF and OE, but AST is not sufficiently accurate. The lack of susceptibility testing for methicillin makes this test inappropriate for use in small animal practice.

Keywords: antimicrobial susceptibility test, dog, otitis externa, point-of-care test, superficial bacterial folliculitis.

Acute and subacute toxicity tests of goat bile in BALB/c mice

Research (Published online: 20-03-2020)

18. Acute and subacute toxicity tests of goat bile in BALB/c mice

Heny Arwati, Windya T. Hapsari, Kartika A. Wardhani, Kholida N. Aini, Ramadhani R. Bahalwan, Puspa Wardhani and Willy Sandhika

Veterinary World, 13(3): 515-520

ABSTRACT

Aim: The aim of this study was to investigate the toxicity of goat bile in BALB/c mice since some Indonesian people consume raw goat gallbladder to treat malaria and increase stamina.

Materials and Methods: Acute toxicity test was done in six groups of BALB/c mice using 100%, 50%, 25%, 12.5%, and 6.75% of goat bile and negative control. The death of mice was observed within 14 days. In the subacute toxicity test, the body weight and hematology parameters on day 0 and day 4 post-treatment were evaluated. The mice were closely observed for 28 days before plasma collection for the blood biochemistry evaluation.

Results: Mild diarrhea was observed in acute and subacute toxicity tests. No death of mice was observed in acute test. Goat bile did not inhibit the increase of the body weight of mice. A slight reduction in hemoglobin and hematocrit levels in mice treated with 25% and 50% goat bile, however, remained normal in mice treated with 100% goat bile. The red and white blood cell count were not affected. Liver and kidney functions were not affected by goat bile treatment as revealed by the plasma level of aspartate aminotransferase and alanine aminotransferase, blood urea nitrogen, and creatinine, which remained in the normal range.

Conclusion: Goat bile treatment in BALB/c mice caused mild toxicity in mice. Hydrophobic bile acids may cause the toxicity of goat bile in mice; therefore, it is recommended that goat bile consumption not to be taken oftenly to avoid its harmful effect.

Keywords: BALB/c mice, goat bile, Indonesia, toxicity.