Meta-analysis of the prevalence of livestock diseases in North Eastern Region of India

Research (Published online: 11-01-2020)

12. Meta-analysis of the prevalence of livestock diseases in North Eastern Region of India

Nagendra Nath Barman, Sharanagouda S. Patil, Rashmi Kurli, Pankaj Deka, Durlav Prasad Bora, Giti Deka, Kempanahalli M. Ranjitha, Channappagowda Shivaranjini, Parimal Roy and Kuralayanapalya P. Suresh

Veterinary World, 13(1): 80-91

ABSTRACT

Aim: The study aimed to determine the overall prevalence of livestock diseases in North Eastern Region (NER) of India, through a systematic review and meta-analysis of published data.

Materials and Methods: The articles used for the study were retrieved from PubMed, J-Gate Plus, Indian Journals, and Google scholar, R open-source scripting software 3.4.3. Metafor, Meta. The Chi-square test was conducted to assess for the heterogeneity, forest plot (confidence interval [CI] plot) is a method utilized to present the results of meta-analysis, displaying effect estimate and their CIs for each study were used for searching and retrieval of livestock diseases prevalence data in India using a search strategy combining keywords and related database-specific subject terms from 2008 to 2017 in English only.

Results: The prevalence of various livestock diseases are foot-and-mouth disease (21%), bluetongue (28%), brucellosis in bovine (17%), brucellosis in caprine (2%), brucellosis in porcine (18%), brucellosis in sheep and goat (3%), babesiosis (6%), theileriosis (26%), porcine reproductive and respiratory syndrome (1%), porcine cysticercosis (6%), classical swine fever (31%), Porcine circovirus (43%), and Peste des petits ruminants (15%). This information helps policymakers to take appropriate measures to reduce the disease burden.

Conclusion: This study indicates that the overall prevalence of various livestock diseases in NER of India.

Keywords: babesiosis, brucellosis, classical swine fever, foot-and-mouth disease, forest plot, livestock, meta-analysis, North Eastern regions, Peste des petits ruminants, Porcine circovirus, porcine cysticercosis, porcine reproductive and respiratory syndrome, prevalence, seroprevalence, theileriosis.

Hypoglycemic efficacy of Rosmarinus officinalis and/or Ocimum basilicum leaves powder as a promising clinico-nutritional management tool for diabetes mellitus in Rottweiler dogs

Research (Published online: 10-01-2020)

11. Hypoglycemic efficacy of Rosmarinus officinalis and/or Ocimum basilicum leaves powder as a promising clinico-nutritional management tool for diabetes mellitus in Rottweiler dogs

Noha Abdelrahman, Ramadan El-Banna, Mahmoud M. Arafa and Maha M. Hady

Veterinary World, 13(1): 73-79

ABSTRACT

Background and Aim: This study examined the impact of dietary fortification with rosemary (Rosmarinus officinalis) and/or basil (Ocimum basilicum) leaves powder on glycemic status of dogs.

Materials and Methods: Forty-five Rottweiler dogs were assigned to five experimental groups and fed an experimentally processed extruded basal diet that was either fortified or not fortified. G1 was fed the basal diet without any fortification (negative control); G2 was consumed the basal diet supplemented with a commercially available synthetic palatant (positive control); G3 was provided with rosemary fortified (at 0.05%) basal diet; G4 was provided with a basil fortified (at 0.05%) basal diet; and G5 was offered a rosemary and basil fortified (each at 0.025%) basal diet.

Results: G4 and G5 exhibited a positive impact on growth performance traits. Dogs in G3, G4, and G5 showed significant decreases in serum glucose levels in comparison to dogs of the control groups (G1 and G2). It was clear that the inclusion level of 0.05% of basil leaves powder showed the greatest hypoglycemic action. Indeed, G4 dogs showed a reduction in blood glucose at a percentage of approximately 31% followed by G5 and G3 groups (16.25% and 14%, respectively). Furthermore, basil leaves inhibited the amylase enzyme activity. Both insulin and cortisol levels in G4 dogs were increased and reduced compared to controls, respectively. In addition, dietary fortification with rosemary and/or basil significantly increased glutathione, superoxide dismutase, and catalase levels, while values for malondialdehyde and lactate dehydrogenase were decreased.

Conclusion: It could be concluded that dietary fortification of dog diet with rosemary and/or basil leaves powder at 0.05% separately or 0.025% in combination might be used as promising modulators of blood glucose levels as well as clinico-nutritional management tools for the prevention and control of diabetes mellitus in dogs.

Keywords: amylase, basil, cortisol, hypoglycemia, insulin, rosemary, Rottweiler.

Canine demodicosis: Hematological and biochemical alterations

Research (Published online: 10-01-2020)

10. Canine demodicosis: Hematological and biochemical alterations

N. Y. Salem, H. Abdel-Saeed, H. S. Farag and R. A. Ghandour

Veterinary World, 13(1): 68-72

ABSTRACT

Background and Aim: One of the most common cutaneous infections seen in veterinary canine practice is canine demodicosis. Demodicosis is a parasitic skin infection with a possible impact on acute-phase proteins (APPs) and oxidant-antioxidant balance. This study aimed to estimate the possible alterations in hematological, biochemical, oxidant-antioxidant, and APP (C-reactive protein [CRP] and albumin) profiles in naturally infected dogs with demodicosis.

Materials and Methods: This study enrolled 21 dogs that were divided into two groups: The control group including 7 apparently healthy dogs and the diseased group including 14 dogs with generalized demodicosis. Demodicosis was confirmed through microscopic detection. Blood samples were collected for the estimation of CBC, total protein, albumin, alanine transaminase, aspartate aminotransferase, blood urea nitrogen, creatinine, superoxide dismutase (SOD), glutathione peroxidase (GPx), total antioxidant capacity (TAC), catalase (CAT), malondialdehyde (MDA), and CRP levels.

Results: Significant reduction in red blood cells along with significant elevation in white blood cells was recorded in the diseased group compared with the control group. There was also significant elevation in MDA, TAC, SOD, and CRP levels along with significant reduction in GSH-Px and CAT levels in the diseased group.

Conclusion: Based on these findings, a relationship between canine generalized demodicosis and oxidant-antioxidant disequilibrium could be suggested. Evidence of this relation manifested in the elevation in MDA and SOD levels and reduction in GPx and CAT levels as a consequence to the release of ROS resulting from Demodex infection. CRP elevation is expected in canine demodicosis.

Keywords: antioxidant enzymes, canine generalized demodicosis, C-reactive protein, malondialdehyde, total antioxidant capacity.

Effect of discriminate and indiscriminate use of oxytetracycline on residual status in broiler soft tissues

Research (Published online: 10-01-2020)

9. Effect of discriminate and indiscriminate use of oxytetracycline on residual status in broiler soft tissues

Most. Rifat Ara Ferdous, Md. Raju Ahmed, Sayekul Hasan Khan, Mufsana Akter Mukta, Tasnia Tabassum Anika, Md. Tarek Hossain, Md. Zahorul Islam and Kazi Rafiq

Veterinary World, 13(1): 61-67

ABSTRACT

Aim: This study aimed to evaluate the effects of discriminate and indiscriminate use of oxytetracycline on hematological parameters, residual status in soft tissue of broiler and of thermal effect on oxytetracycline residual status.

Materials and Methods: Eighteen, day-old male broiler chickens were purchased and were divided into three different groups (control group, discriminate group, and indiscriminate group). The control group received no antibiotics. The discriminate group received oxytetracycline 1 g/L drinking water for 5 consecutive days, and 10 days' withdrawal period was maintained before sacrifice. The indiscriminate group received oxytetracycline 1 g/L drinking water till the sacrificed day. Blood samples were collected before sacrificing for hematological analysis. After sacrificing liver, kidney, spleen, and muscle samples were collected for analysis of oxytetracycline residues in raw soft tissues. Since meat is used to cook by traditional method in Bangladesh before consumption that is why positive meat samples were cooked by traditional cooking method to evaluate the thermal effect on oxytetracycline residual status as well. Thin-layer chromatography (TLC) was done for screening of oxytetracycline residues in soft tissues.

Results: Mean differences of total erythrocyte count (million/mm3), hemoglobin estimation (gm%), and packed cell volume (%) estimation were not statistically significant among the groups. TLC analysis of raw samples showed 100% positive results of all samples collected from the indiscriminate group. In contrast, samples collected from the discriminate group were negative for oxytetracycline residues. In the control group, all samples were negative for oxytetracycline residue. There was a significant (p<0.05) relationship of oxytetracycline residues among three different groups for liver, kidney, spleen, and muscle samples. Positive liver and muscle samples from the indiscriminate group were subjected to thermal treatment by traditional cooking method of Bangladesh. Oxytetracycline residues had found in cooked meat, liver, and juice part, suggesting that antibiotic residues disseminated to juice part from flesh part after cooking.

Conclusion: Evidence suggests that proper maintenance of withdrawal period would minimize oxytetracycline residues in broiler soft tissues, whereas antibiotics retained in soft tissues of broiler in case of indiscriminate use. Traditional cooking does not change oxytetracycline residual status in edible tissues. Therefore, awareness regarding the proper maintenance of withdrawal period after antibiotic treatment of broiler is one of the best strategies which may positively reduce the risk of antimicrobial drugs residue in meat.

Keywords: antibiotic residue, broiler edible tissues, oxytetracycline, thermal effect, thin-layer chromatography.

Toxoplasma gondii seropositivity and the associated risk factors in sheep and pregnant women in El-Minya Governorate, Egypt

Research (Published online: 09-01-2020)

8. Toxoplasma gondii seropositivity and the associated risk factors in sheep and pregnant women in El-Minya Governorate, Egypt

Abdelbaset E. Abdelbaset, Maha I. Hamed, Mostafa F. N. Abushahba, Mohamed S. Rawy, Amal S. M. Sayed and Jeffrey J. Adamovicz

Veterinary World, 13(1): 54-60

ABSTRACT

Background and Aim: The cosmopolite protozoan, Toxoplasma gondii, has a significant economic and medical impact. Cats traditionally play a predominant role in the disease maintenance cycle; however, humans can be infected as a result of milk and meat consumption of Toxoplasma-infected livestock. In addition, infected pregnant women, even symptomless, can pass the disease to their unborn fetus. The limited clinical records and absence of specific national educational programs in countries like Egypt underscore the need for periodic toxoplasmosis disease evaluation. Here, we identified T. gondii seroprevalence among sheep and pregnant women and the associated risk factors in El-Minya Governorate, Egypt.

Materials and Methods: Using peripheral blood, we detected T. gondii-specific antibodies in 151 sheep and 96 pregnant women sera from El-Minya Governorate using latex agglutination and indirect enzyme-linked immunosorbent assay. The impact of different environmental and behavioral risk factors identified with in-person interviews and serology results on acquiring toxoplasmosis was statistically analyzed.

Results: The overall toxoplasmosis seroprevalence was 39.1% and 22.9% in sheep and pregnant women, respectively. Significantly higher seroprevalence was correlated with increasing sheep age and geographical location. Nonetheless, no statistical significance was found based on abortion history and pregnancy status of the examined sheep. Exposure factors important for pregnant women included pregnancy trimester, contact with cats, and the habit of eating undercooked sheep meat, which all had a statistically significant association with Toxoplasma seropositivity.

Conclusion: The current study confirms increased antibodies against toxoplasmosis in both sheep and pregnant women in El-Minya Governorate and a clear association between women's age, contact with cats, and the habit of eating undercooked sheep meat and seroreactivity to T. gondii. These results strongly suggest the need for a more comprehensive epidemiological study and public health awareness education for toxoplasmosis.

Keywords: abortion, Egypt, risk factors, sheep, toxoplasmosis, zoonoses.

Effect of Nauclea subdita (Korth.) Steud. leaf extract on hematological and histopathological changes in liver and kidney of striped catfish infected by Aeromonas hydrophila

Research (Published online: 09-01-2020)

7. Effect of Nauclea subdita (Korth.) Steud. leaf extract on hematological and histopathological changes in liver and kidney of striped catfish infected by Aeromonas hydrophila

Siti Aisiah, Arief Prajitno, Maftuch Maftuch and Ating Yuniarti

Veterinary World, 13(1): 47-53

ABSTRACT

Aim: The present study was conducted to investigate the efficacy of different doses of Bangkal (Nauclea subdita) leaf extract on hematological and histological changes in kidney and liver of catfish (Pangasius hypophthalmus) infected by Aeromonas hydrophila.

Materials and Methods: Catfish were experimentally infected with A. hydrophila at a dose of 108 cells/mL through intraperitoneal injection, and the hematological and histological changes in the kidney and liver of catfish against the pathogen were observed.

Results: Not all concentrations of N. subdita caused a toxic effect in striped catfish. The clinical symptoms of catfish after infection with A. hydrophila and treatment with N. subdita leaf extract included morphological and behavioral changes. N. subdita leaf extract reduced mortality caused by A. hydrophila. Treatment with N. subdita leaf extract was effective in reducing the inflammation by decreasing the activity of neutrophils, monocytes, and lymphocytes. The Hb and Ht levels of catfish significantly decreased after exposure to 108 cells/mL of A. hydrophila and increased significantly after N. subdita treatment. Necrosis percentages in the kidney and liver also decrease after N. subdita treatment.

Conclusion: The results indicate that N. subdita leaf extract stimulates the immunity and increases the resistance of catfish to A. hydrophila. N. subdita leaf extract may be used as a potential source for future drug development and food applications.

Keywords: Aeromonas hydrophila, hematology, histopathology, Nauclea subdita.

Forecasting head lice (Pediculidae: Pediculus humanus capitis) infestation incidence hotspots based on spatial correlation analysis in Northwest Iran

Research (Published online: 06-01-2020)

6. Forecasting head lice (Pediculidae: Pediculus humanus capitis) infestation incidence hotspots based on spatial correlation analysis in Northwest Iran

Davoud Adham, Eslam Moradi-Asl, Malek Abazari, Abedin Saghafipour and Parisa Alizadeh

Veterinary World, 13(1): 40-46

ABSTRACT

Background and Aim: Pediculus humanus capitis has been prevalent throughout the world, especially in developing countries among elementary students and societies with a weak socio-economic status. This study aimed to forecast head lice (Pediculidae: P. capitis) infestation incidence hotspots based on spatial correlation analysis in Ardabil Province, Northwest Iran.

Materials and Methods: In this retrospective analytical study, all cases of head lice infestations who were confirmed by Centers for Disease Control office have been studied from 2016 to 2018. Head lice infestation incidence hotspots in the province should be detected based on general G statistics in ArcMap GIS10.4.1. Furthermore, MaxEnt.3.3.3 model was used for modeling the high-risk areas.

Results: The prevalence rate of pediculosis was 14.90/100,000 populations. The general G statistics revealed that the head lice infestation in this study area has a high cluster pattern. The analysis showed that the Parsabad and Germi counties were identified as a head lice infestation incidence hotspots. Statistical and spatial analyses of head lice infestation incidence showed a significant positive correlation with head lice infestation incidence hotspots and the altitudes (15-500 m), annual temperature range (14-16.5°C), and slope and average diurnal temperature (12-18°C).

Conclusion: The results of this study showed that the most ecologically suitable areas of head lice occurrence were identified in two hotspots (Parsabad and Germi) in the Northern areas of Ardabil Province (Parsabad and Germi counties); in the borderline of Iran and the Republic of Azerbaijan.

Keywords: head lice infestation, Iran, Pediculus humanus capitis, spatial analysis.