Pasteurellaceae members with similar morphological patterns associated with respiratory manifestations in ducks

Research (Published online: 26-12-2019)

24. Pasteurellaceae members with similar morphological patterns associated with respiratory manifestations in ducks

Samah Eid, Sherif Marouf, Hefny Y. Hefny and Nayera M. Al-Atfeehy

Veterinary World, 12(12): 2061-2069

ABSTRACT

Aim: A total of 112 freshly dead ducks aged from 2 to 20 weeks old with a history of respiratory manifestations were investigated for the implication of Pasteurellaceae family members..

Materials and Methods: Isolation and identification to the family level were conducted by conventional bacteriological methods, including microscopic examination and biochemical characterization. Identification to the species level was conducted by polymerase chain reaction (PCR) and analytical profile index (API) 20E kits.

Results: Conventional bacteriological isolation and biochemical characterization revealed the infection of 16/112 examined birds with a prevalence rate of 14.3%. PCR confirmed the detection of Pasteurellaceae family conserved genes RpoB and Bootz in 16/16 (100%) isolates. PCR was also used for genus and species identification of the isolated Pasteurellaceae members; the results revealed that 5/16 (31.3%) of isolates were Gallibacterium anatis and 2/16 of isolates (12.5%) were Pasteurella multocida. Riemerella anatipestifer, Mannheimia haemolytica, and Avibacterium paragallinarum were not detected by PCR. Biotyping by API 20E successfully identified 5/16 (31.3%) isolates that could not be typed by PCR and confirmed their belonging to Pasteurella pneumotropica. Neither the available PCR primer sets nor API 20E succeeded for species identification of 4/16 (25%) isolates. Antibiotic susceptibility profiling of isolates revealed that 16/16 (100%) of isolates demonstrated multidrug resistance (MDR) phenotypes. Moreover, 16/16 (100%) of isolates demonstrated a phenotypic resistance pattern to neomycin.

Conclusion: Combined genotypic, phenotypic, biotyping, and virulence characterizations are required for laboratory identification of pathogenic Pasteurellaceae. Moreover, P. multocida was not the prevailed member implicated in respiratory problems in ducks as P. pneumotropica, G. anatis, and unidentified strains were involved with higher prevalence. Chloramphenicol and ampicillin demonstrated the highest in vivo effects on the studied Pasteurellaceae. Furthermore, the prevalence of multidrug-resistant isolates signified the demand to implement targeted surveillance in the ducks' production sector, and MDR survey in poultry sectors in Egypt to apply effective control measures.

Keywords: ducks, Gallibacterium anatis, Mannheimia haemolytica, Pasteurella multocida, Riemerella anatipestifer.

Combined virgin coconut oil and tocotrienol-rich fraction protects against bone loss in osteoporotic rat model

Research (Published online: 25-12-2019)

23. Combined virgin coconut oil and tocotrienol-rich fraction protects against bone loss in osteoporotic rat model

Mohd Maaruf Abdul Malik, Faizah Othman, Farida Hussan, Ahmad Nazrun Shuid and Qodriyah Mohd Saad

Veterinary World, 12(12): 2052-2060

ABSTRACT

Background and Aim: Both virgin coconut oil (VCO) and tocotrienol-rich fraction (TRF) are rich in antioxidants and may protect the bone against bone loss induced by ovariectomy and high-fat diet. The study aimed to determine the protective effects of combined therapy of VCO and TRF on osteoporosis in ovariectomized (OVX) rat fed with high-fat diet.

Materials and Methods: Thirty-six female Sprague-Dawley rats were divided into six groups: Sham-operated (SHAM), OVX control, OVX and given Premarin at 64.5 μg/kg (OVX+E2), OVX and given VCO at 4.29 ml/kg (OVX+V), OVX and given TRF at 30 mg/kg (OVX+T), and OVX and given a combination of VCO at 4.29 ml/kg and TRF at 30 mg/kg (OVX+VT). Following 24 weeks of treatments, blood and femora samples were taken for analyses.

Results: There were no significant differences in serum osteocalcin levels between the groups (p>0.05), while serum C-terminal telopeptide of Type I collagen levels of the OVX+VT group were significantly lower than the other groups (p<0.05). The dynamic bone histomorphometry analysis of the femur showed that the double-labeled surface/bone surface (dLS/BS), mineral apposition rate, and bone formation rate/BS of the OVX+E2, OVX+T, and OVX+VT groups were significantly higher than the rest of the groups (p<0.05).

Conclusion: A combination of VCO and TRF has the potential as a therapeutic agent to restore bone loss induced by ovariectomy and high-fat diet.

Keywords: bone loss, osteoporosis, ovariectomized rat, ovariectomy, tocotrienol-rich fraction, virgin coconut oil.

Prebiotic activity of garlic (Allium sativum) extract on Lactobacillus acidophilus

Research (Published online: 24-12-2019)

22. Prebiotic activity of garlic (Allium sativum) extract on Lactobacillus acidophilus

Prayogi Sunu, Dwi Sunarti, Luthfi Djauhari Mahfudz and Vitus Dwi Yunianto

Veterinary World, 12(12): 2046-2051

ABSTRACT

Aim: The study aimed to examine the ability of prebiotic concentrations to increase the growth of probiotic bacteria in vitro.

Materials and Methods: The probiotics used were Lactobacillus acidophilus and garlic (Allium sativum) extract.

Results: The results showed that garlic can increase the growth of L. acidophilus bacteria with the lowest concentration of 4% being the most effective (p<0.05). Increased fructooligosaccharide (FOS) content in garlic can increase the significant growth of L. acidophilus as a probiotic bacterium.

Conclusion: The results showed that garlic can increase the growth of L. acidophilus bacteria by a minimum of 4% (p<0.05). Adding FOS to garlic can increase the significant growth of L. acidophilus as a probiotic bacterium.

Keywords: Allium sativum, prebiotics, probiotics, synbiotics.

Comparative modeling and mutual docking of structurally uncharacterized heat shock protein 70 and heat shock factor-1 proteins in water buffalo

Research (Published online: 23-12-2019)

21. Comparative modeling and mutual docking of structurally uncharacterized heat shock protein 70 and heat shock factor-1 proteins in water buffalo

Ravinder Singh, Ankita Gurao, C. Rajesh, S. K. Mishra, Saroj Rani, Ankita Behl, Vikash Kumar and R. S. Kataria

Veterinary World, 12(12): 20236-2045

ABSTRACT

Aim: In this study, a wide range of in silico investigation of Bubalus bubalis (BB) heat shock protein 70 (HSP70) and heat shock factor-1 (HSF1) has been performed, ranging from sequence evaluation among species to homology modeling along with their docking studies to decipher the interacting residues of both molecules.

Materials and Methods: Protein sequences of BB HSP70 and HSF1 were retrieved from NCBI database in FASTA format. Primary and secondary structure prediction were computed using Expasy ProtParam server and Phyre2 server, respectively. TMHMM server was used to identify the transmembrane regions in HSP70. Multiple sequence alignment and comparative analysis of the protein was carried out using MAFFT and visualization was created using ESPript 3.0. Phylogenetic analysis was accomplished by COBALT. Interactions of HSP70 with other proteins were studied using STRING database. Modeller 9.18, RaptorX, Swiss-Modeller, Phyre2, and I-TASSER were utilized to design the three-dimensional structure of these proteins followed by refinement; energy minimization was accomplished using ModRefiner and SPDBV program. Stereochemical quality along with the accuracy of the predicted models and their visualization was observed by PROCHECK program of PDBsum and UCSF Chimera, respectively. ClusPro 2.0 server was accessed for the docking of the receptor protein with the ligand.

Results: The lower value of Grand Average of Hydropathy indicates the more hydrophilic nature of HSP70 protein. Value of the instability index (II) classified the protein as stable. No transmembrane region was reported for HSP70 by TMHMM server. Phylogenetic analysis based on multiple sequence alignments (MSAs) by COBALT indicated more evolutionarily closeness of Bos indicus (BI) with Bos taurus as compared to BI and BB. STRING database clearly indicates the HSF1 as one of the interacting molecules among 10 interacting partners with HSP 70. The best hit of 3D model of HSP70 protein and HSF1 was retrieved from I-TASSER and Phyre2, respectively. Interacting residues and type of bonding between both the molecules which were docked by ClusPro 2.0 were decoded by PIC server. Hydrophobic interactions, protein-protein main-chain-side-chain hydrogen bonds, and protein-protein side-chain-side-chain hydrogen bonds were delineated in this study.

Conclusion: This is the first-ever study on in silico interaction of HSP70 and HSF1 proteins in BB. Several bioinformatics web tools were utilized to study secondary structure along with comparative modeling, physicochemical properties, and protein-protein interaction. The various interacting amino acid residues of both proteins have been indicated in this study.

Keywords: Bubalus bubalis, docking, heat shock proteins, heat shock factor-1, heat shock protein 70, homology modeling.

---

Treatment of experimentally induced diabetic wound infected with methicillin-resistant Staphylococcus aureus using Aloe vera, Apium graveolens, and Sauropus androgynus extracts in rats

Research (Published online: 23-12-2019)

15. Treatment of experimentally induced diabetic wound infected with methicillin-resistant Staphylococcus aureus using Aloe vera, Apium graveolens, and Sauropus androgynus extracts in rats

Yos Adi Prakoso, Kurniasih Kurniasih, Agustina Dwi Wijayanti and Yuli Purwandari Kristianingrum

International Journal of One Health, 5: 99-106

ABSTRACT

Background and Aim: One of the complications of diabetes mellitus is diabetic ulcer. Diabetic ulcer is commonly infected by infectious agents, especially methicillin-resistant Staphylococcus aureus (MRSA). This study aimed to evaluate the potential effects of alcoholic extracts of Aloe vera, Apium graveolens, and Sauropus androgynus on promoting wound healing in a diabetic wound infected with MRSA.

Materials and Methods: A total of 60 male Sprague-Dawley rats (6 months old, weighing 250-300 g) were injected with 65 mg/kg body weight of streptozotocin to induce diabetes. On day 7, the backs of the rats were shaved, and two circular wounds (4 mm in diameter) were created on their back, which were infected with MRSA. The rats were divided into six groups: Group I = control, Group II = treated with cream base without extract, Group III = treated with 2% A. vera cream, Group IV = treated with 2% A. graveolens cream, Group V = treated with 2% S. androgynus cream, and Group VI = treated with 2% A. vera + 2% A. graveolens + 2% S. androgynus cream. The wounds were treated twice a day for 14 days. The data were collected on days 7 and 14.

Results: The results showed that all three herbal extracts and their combination decreased wound area and percentage of the wound, increased tensile strength of skin, collagen deposition, vascular endothelial growth factor expression, and skin thickness, and depressed the C-reactive protein profile and cyclooxygenase-2 expression.

Conclusion: A. vera, A. graveolens, and S. androgynus creams can be used as herbal therapies against diabetic wounds infected with MRSA, both as a single and combination treatment.

Keywords: Aloe vera, Apium graveolens, diabetes ulcer, methicillin-resistant Staphylococcus aureus, Sauropus androgynus.

Evaluation of the efficacy of commonly used disinfectants against isolated chlorine-resistant strains from drinking water used in Egyptian cattle farms

Research (Published online: 21-12-2019)

20. Evaluation of the efficacy of commonly used disinfectants against isolated chlorine-resistant strains from drinking water used in Egyptian cattle farms

Mohamed Abdelhameed Kamal, Mahmoud Abdelaty Khalaf, Zakia Attia Mohamed Ahmed and Jakeen El Jakee

Veterinary World, 12(12): 2025-2035

ABSTRACT

Background and Aim: Drinking water of poor microbiological quality contains high percentages of microbes causing outbreaks of mainly coliform-related diseases. These microbes could be controlled by many hygienic standards including disinfection, but disinfectants misuse causes the developing of disinfectant-resistant strains. The present study aimed to investigate drinking water bacterial profile, determine chlorine-resistant strains, and statistically correlate that with the used disinfectant and disinfection process variables. In vitro evaluation of the bactericidal effect of the most commonly used disinfectants in cattle operations against the isolated chlorine-resistant strains and detection of qacE resistance gene in the isolated chlorine-resistant Escherichia coli strains in some cattle farms suffering coliform and non-coliform related disease around Egypt.

Materials and Methods: A structured questionnaire is used to survey a convenience sample of 132 Egyptian cattle beef and dairy farms suffering emerged epidemics to identify commonly used disinfection process, disinfectant types, disinfectants frequency, and rate of use. One hundred and thirty-two water samples were collected for microbiological analysis to obtain water bacterial profile and testing resistance to chlorine. Statistical analysis was performed to identify the level of association between microbial profile and presence of chlorine-resistant strains in each farm with used disinfection, disinfectant types, and rate of use in these farms.

Results: A wide range of disinfectant types used for variable purposes inside cattle farms with a different frequency of use and the highest percent of farms 25.8% use 4-5 types of disinfectants, followed by 25% of farms use two types, then 18.9% use three types. Microbial profile of water samples revealed isolation of E. coli, Streptococcus faecalis, Pseudomonas aeruginosa, Klebsiella spp., Proteus spp., Salmonella spp., Enterobacter spp., Citrobacter spp., Shigella flexneri, Serratia marcescens, and Yersinia enterocolitica in percent (98.5, 97.7, 97.7, 76.5, 66.7, 36.4, 78.8, 74.2, 30.3, 29.5, and 14.4% of cattle farms, respectively), from which five E. coli, four Salmonella, four Pseudomonas, two Klebsiella, and four Streptococcus strains expressed chlorine resistance. Statistical analysis showed weak to moderate correlation (rho 0.15-0.46) between bacterial profile strains count and presence of resistant strains with different farm disinfection, disinfectant types, and rate of use. Experimental evaluation of the bactericidal effect of the eight selected disinfectants on the chlorine-resistant isolated strains revealed that peroxymonosulfate killed 19/19 isolated strains/15 min contact time, and quaternary ammonium compounds killed only 3/19 strains/15 min contact time. The qacE resistance gene was detected in 3/4 isolated chlorine-resistant E. coli strains.

Conclusion: Drinking water microbial profile strains and resistance to disinfectants are widely varied in cattle farms, and this variance depends on critical factors among which the disinfection process types used disinfectant types and frequency of disinfectants use or change.

Keywords: cattle farms, disinfectant resistance, drinking water, microbial profile, resistance genes.

Small ruminants as a pathway to reduce urban poverty: An empirical analysis of Sudan

Research (Published online: 20-12-2019)

19. Small ruminants as a pathway to reduce urban poverty: An empirical analysis of Sudan

Raga Elzaki, Samar Abdalla and Mohammed Al-Mahish

Veterinary World, 12(12): 2017-2024

ABSTRACT

Aim: This study aimed to measure the energetic incidence of poverty and determines the main factors that cause urban poverty. Moreover, the study examines the key role of the livestock sector in poverty reduction in urban regions and develops an analytical tool to aid in urban area poverty mitigation through goats and sheep ownership.

Materials and Methods: The study mainly depends on primary data assembled through structured and unstructured questionnaires, which were distributed among the targeted groups in the urban area in Sudan. Poverty line and poverty indices were calculated and measured using various well-known methods. The causes of poverty were estimated using logistic regression, and the effect of small ruminants in poverty alleviation was estimated using multivariate regression analysis.

Results: The study findings indicate that both food and income poverty lines are less than the standard poverty line. In addition, the results imply that rural migration and crime predictors are among the most important factors in increasing urban poverty in the study area. Furthermore, livestock ownership has a significant impact on poverty reduction.

Conclusion: The study concludes that small ruminants are playing a key role in reducing urban poverty. Thus, the study urges planners and policy-makers to support policies that promote livestock sector development as a strategy to alleviate poverty in Sudan.

Keywords: goats, sheep, urban poverty causes, urban poverty line, urban poverty reduction.