Wednesday, 11 December 2019

Characterization and molecular epidemiology of Staphylococcus aureus strains resistant to beta-lactams isolated from the milk of cows diagnosed with subclinical mastitis

Research (Published online: 11-12-2019)
7. Characterization and molecular epidemiology of Staphylococcus aureus strains resistant to beta-lactams isolated from the milk of cows diagnosed with subclinical mastitis
Geziella Áurea Aparecida Damasceno Souza, Anna Christina de Almeida, Mauro Aparecido de Sousa Xavier, Lívia Mara Vitorino da Silva, Cintya Neves Sousa, Demerson Arruda Sanglard and Alessandra Rejane Ericsson de Oliveira Xavier
Veterinary World, 12(12): 1931-1939
ABSTRACT
Background and Aim: The term ESKAPE, recognized by the WHO, is an acronym, which refers to the pathogens Enterococcus faeciumStaphylococcus aureusKlebsiella pneumoniaeAcinetobacter baumanniiPseudomonas aeruginosa, and Enterobacter spp., which is extremely virulent and multidrug-resistant. Although the term is used to designate nosocomial pathogens, in a milking environment, strains of Methicillin-resistant S. aureus have been isolated from cattle diagnosed with clinical and subclinical mastitis. Resistant strains may be involved in the transfer of genes conferring resistance to beta-lactam antimicrobials among the species of microorganisms related to mastitis etiology. This study aimed to trace the phenotypic and genotypic profiles of susceptibility to beta-lactams in S. aureus isolated from milk of cattle diagnosed with subclinical mastitis obtained from different rural properties located in the North of Minas Gerais State, Brazil.
Materials and Methods: Sixteen microorganisms previously identified as S. aureus isolated from milk of cattle diagnosed with subclinical mastitis were submitted to matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF), mass spectrometry, and polymerase chain reaction (PCR) analysis for microbial species confirmation. The S. aureus beta-lactams antimicrobial phenotypic resistance profile was investigated by disk diffusion method. PCR methods were also performed to investigate the S. aureus genotypic beta-lactams resistance profile. For this purpose, blaZmecA, mecALGA251blaOxa23, and blaKPC genes were screened among S. aureus isolates. The genetic diversity of S. aureus by fingerprint random amplified polymorphic DNA (RAPD)-PCR was also performed in this study.
Results: All isolates showed phenotypic resistance to at least three beta-lactams, among which was meropenem. None of the isolates tested positive for the genes mecALGA251blaOxa23, and blaKPC; however, the presence of the genes blaZ and mecA was detected among the isolates. The fingerprint analysis divided isolates into two distinct groups and 15 different subgroups. Despite the presence of clonality among the isolates, the PCR-RAPD analysis unveiled a heterogeneous profile with genetic diversity among the S. aureus isolates.
Conclusion: In this study, we identified beta-lactams resistant S. aureus strains isolated from the milk of cows diagnosed with subclinical mastitis. The S. aureus beta-lactams resistance was investigated using a phenotypic and genotypic approach. We believe that molecular epidemiology, improved knowledge, and genetic basis of resistance to beta-lactams might assist in asserting guidelines for better management practices of dealing with subclinical mastitis and mapping of origin of resistant pathogens in the studied Brazilian area.
Keywords: beta-lactams, genetic diversity, infection, resistance genes, Staphylococcus aureus.

Capripoxviruses: Exploring the genetic relatedness between field and vaccine strains from Egypt

Research (Published online: 11-12-2019)
6. Capripoxviruses: Exploring the genetic relatedness between field and vaccine strains from Egypt
Sherin Reda Rouby, Abdel-Hamid Bazid, Momtaz Wasfy and Magdy El-Sayed
Veterinary World, 12(12): 1924-1930
ABSTRACT
Background and Aim: Lumpy skin disease (LSD) and sheep pox are economically important Capripoxvirus-induced diseases of cattle and sheep, respectively. Despite the extensive vaccination program adopted by Egyptian veterinary authorities, LSD and sheep pox are still prevalent and spread throughout the whole country. The current study was designed for molecular characterization and phylogenetic analysis of LSD virus (LSDV) and Sheep pox virus (SPPV) recovered from field cases in Egypt along with vaccinal strains to assess their genetic relatedness.
Materials and Methods: Skin biopsies were collected from naturally infected cases of LSD in Ismailia (n=3 farms) and Beni-Suef (n=2 farms) Governorates and sheep pox in Beni-Suef (n=1 flock). Virus isolation was carried out on primary ovine fetal kidney and heart cell cultures. DNA was extracted from infected materials (skin lesions, infected cell cultures) as well as LSDV Neethling vaccine strain and Romanian SPPV vaccine strain. Polymerase chain reaction was performed using oligonucleotide primers targeting the entire open reading frame of G protein-coupled receptors (GPCR) gene and gene sequences were analyzed.
Results: Virus isolation on primary ovine fetal kidney and heart cell culture revealed a cytopathic effect at the third passage characterized by rounding of infected cells and margination of nuclear chromatin. Comparative sequence analysis of GPCR gene revealed that Egyptian LSDV isolated from Ismailia and Beni-Suef shared 99:100% nucleotide and amino acid (AA) identities with each other. In comparison to the vaccinal strains, Egyptian LSDV isolates shared 98:99 nucleotide and AA identities with LSDV Neethling vaccine strain and 93:94% with SPPV Romanian vaccine strain. No differences at the nucleotide or AAs were observed between the SPPV vaccine and virulent strains (100% identity). Phylogenetic analyses revealed that LSDV Neethling vaccine strain is more related to field Egyptian LSDV and clustered within the LSDV group while Romanian SPPV vaccine strain clustered in a separate clade with SPPV field isolates.
Conclusion: Comparative sequencing and phylogenetic analyses of the GPCR gene reveal a minimal genetic variation between LSDV field isolates from different locations and a close relationship between virulent field strains and homologous vaccines.
Keywords: Capripoxvirus, G protein-coupled receptors gene, phylogenetic, Romanian.

Tuesday, 10 December 2019

Antioxidant, hypoglycemic, and hepatoprotective effect of aqueous and ethyl acetate extract of carob honey in streptozotocin -induced diabetic rats

Research (Published online: 10-12-2019)
5. Antioxidant, hypoglycemic, and hepatoprotective effect of aqueous and ethyl acetate extract of carob honey in streptozotocin -induced diabetic rats
Redouan El-Haskoury, Noori Al-Waili, Jaouad El-Hilaly, Waili Al-Waili and Badiaa Lyoussi
Veterinary World, 12(12): 1916-1923
ABSTRACT
Aim: The aim of the study included the effect of aqueous extract (AE) and ethyl acetate extract (EAE) on blood sugar in diabetic rats and their effects on liver enzymes and lipid panel in control and diabetic rats. Furthermore, the antioxidant activity of the EAE was studied in vitro and compared with AE.
Materials and Methods: Sugar and antioxidant content of AE and EAE were determined. In vitro antioxidant activity of AE and EAE was estimated by 2, 2-diphenyl-1-picrylhydrazyl and ABTS*+ radical scavenging assay, ferric-reducing antioxidant power assay, and total antioxidant assay. To study the effect of the extracts on blood glucose level (BGL), lipid profile, and liver function in non-diabetic and diabetic rats, five groups of six rats each were treated with distilled water, AE, EAE, glibenclamide (GLB), and sucrose for 8 days. Plasma glucose level (PGL), total cholesterol (TC), triglycerides (TG), transaminases (alanine transaminase [ALT] and aspartate transaminase [AST]), and alkaline phosphatase (ALP) were determined. The effect of the interventions on BGL after acute administration also was investigated. Diabetes was induced by streptozotocin injection.
Results: EAE contains significantly lower content of fructose and glucose than AE (p<0.05), and it has no sucrose. AE and EAE exhibited a significant antioxidant activity and high antioxidant content; the antioxidant content was higher in AE than EAE (p<0.05). In diabetic rats, acute treatment by AE increased PGL, while EAE significantly lowered BGL as compared to the untreated diabetic rats. Both interventions significantly decreased BGL as compared to the sucrose treated group in diabetic rats (p<0.05). EAE was more potent than GLB. Sucrose caused 13% increment in BGL after 8 days of induction of diabetes, while AE caused only 1.3% increment. Daily treatment by EAE decreased significantly AST, ALT, ALP, and TC. EAE decreased significantly TC and TG level in diabetic rats in comparison to the untreated diabetic group.
Conclusion: The study showed for the 1st time that EAE has more hypoglycemic effect than AE, and both extracts prevent the increment in BGL on day 8 after induction of diabetes observed in the control and sucrose treated group. EAE significantly ameliorated the lipid and liver function disorders induced by diabetes.
Keywords: carob honey, diabetes, ethyl acetate extract, glucose, lipid, transaminases.

Saturday, 7 December 2019

Coprological detection of equine nematodes among slaughtered donkeys (Equus asinus) in Kaltungo, Nigeria

Research (Published online: 07-12-2019)
4. Coprological detection of equine nematodes among slaughtered donkeys (Equus asinus) in Kaltungo, Nigeria
Tobias Nnia Egbe-Nwiyi, Bura Thlama Paul and Ajuji Chungsyn Cornelius
Veterinary World, 12(12): 1911-1915
ABSTRACT
Aim: This study aimed to investigate the prevalence and intensity of nematode infection among slaughtered donkeys in Kaltungo, Nigeria.
Materials and Methods: A total of 72 fecal samples were examined by salt flotation and the modified McMaster fecal egg count technique to morphologically identify nematodes eggs and determine their egg per gram (EPG) outputs.
Results: Out of a total of 72 (100%) donkeys sampled, 36 (50%) tested positive, but the prevalence of nematodes was independent of the age, sex, and breed of donkeys (p>0.05). Among the four species of nematodes identified in single and mixed infections, Strongylus spp. (27.8%) and Dictyocaulus arnfieldi (13.9%) were the most prevalent followed by Strongyloides westeri (5.6%) and Trichonema spp. (5.6%). Infected donkeys had moderate overall mean EPG (801.39±611.3) with no statistical differences between age groups and sexes (p>0.05), but means of EPG were significantly higher (p<0.05) in Duni (1026.92±719.55) than Idabari (673.91±514.75). Light EPG count was recorded among 63.9% of infected donkeys, while 16.7% and 19.4% had moderate and severe infections, respectively.
Conclusion: The prevalence and importance of equine nematodes were discussed in connection to their epidemiology and control. Furthermore, the preponderance of light infection may suggest that donkeys in this environment developed resistance to nematode infection and are potential reservoirs for other equines.
Keywords: detection, donkey, egg per gram, Gombe, nematodes, Nigeria.

Wednesday, 4 December 2019

Hepatoprotective and immunomodulatory effects of copper-nicotinate complex against fatty liver in rat model

Research (Published online: 04-12-2019)
3. Hepatoprotective and immunomodulatory effects of copper-nicotinate complex against fatty liver in rat model
Ahmed Medhat Hegazy, Ayman Samir Farid, Ahmed S. Hafez, Rania M. Eid and Soad M. Nasr
Veterinary World, 12(12): 1903-1910
ABSTRACT
Aim: The current study was designed to evaluate the potential hepatoprotective and immunomodulatory effects of copper-nicotinate complex (CNC) against methionine- and choline-deficient diet (MCDD)-induced fatty liver in rats.
Materials and Methods: Forty male Wistar rats were randomly allocated into one of four equal-sized groups (G1-G4). The G1 group was fed a balanced diet and kept under normal conditions; the G2 group received CNC orally at a dose of 0.043 mg/kg body weight, 3 times/week for 4 weeks, and a balanced diet; the G3 group was fed an MCDD for 4 weeks; and the G4 group was fed an MCDD and administered CNC at the same dose and route as G2. Blood samples were collected for the determination of serum enzyme activity. After 4 weeks of treatment, liver specimens were collected for the evaluation of the oxidative/antioxidative markers, cytokine gene expression, and histopathological examination.
Results: CNC improved MCDD-induced liver dysfunctions by recovering serum alanine aminotransferase, aspartate aminotransferase, and gamma-glutamyl transferase activities to their normal levels. The glutathione (GSH) level and superoxide dismutase (SOD) activity significantly decreased, while lipid peroxidation (as reflected by malondialdehyde [MDA]) markedly increased in the liver tissue of the MCDD group. After cotreatment with MCDD and CNC, the GSH level and SOD activity markedly increased and the MDA level significantly decreased to return to normal levels. After cotreatment with MCDD and CNC, significant downregulation of the mRNA expression of hepatic interleukin (IL)-1β, IL-4, macrophage inflammatory protein-1a, and monocyte chemoattractant protein-1 genes was found. Moreover, CNC reduced fatty liver complications by reducing the number of hepatic vacuolations, degenerative changes in the hepatocytes, and hemorrhage.
Conclusion: CNC has the potential to limit tissue injury and possibly prevent the progression to severe liver disease caused by an MCDD.
Keywords: copper-nicotinate complex, cytokines gene expression, fatty liver, hepatoprotective, oxidative stress markers.

Tuesday, 3 December 2019

Effects of dichloromethane Sarcophyton spp. extract on the lipopolysaccharide-induced expression of nuclear factor-kappa B and inducible nitric oxide synthase in mice

Research (Published online: 03-12-2019)
2. Effects of dichloromethane Sarcophyton spp. extract on the lipopolysaccharide-induced expression of nuclear factor-kappa B and inducible nitric oxide synthase in mice
Putut Har Riyadi, Didik Wahyudi and Wendy Alexander Tanod
Veterinary World, 12(12): 1897-1902
ABSTRACT
Background and Aim: The soft coral genus Sarcophyton is a source of cembraneterpen. Sarcophyton is reported to have anti-inflammatory properties, with the ability to reduce the expression of inducible nitric oxide synthase (iNOS) and inhibit nuclear factor-kappa B (NF-κB) activation. This study aimed to investigate the efficacy of dichloromethane (DCM) extracts of soft coral Sarcophyton spp. to inhibit the expression of NF-κB and iNOS induced by lipopolysaccharide (LPS).
Materials and Methods: Crude extracts of Sarcophyton spp. were macerated with DCM (1:3 v/v) for 24 h. Thirty-six Balb/c mice were divided into six treatment groups, namely, normal control (without LPS induction), negative control (LPS induction 4 mg/mL), comparative control (LPS+Dexamethasone 6 mg/kg), and 3 concentration groups extract (LPS+50, 125, and 250 mg/kg). The expression of NF-κB and iNOS was measured in each treatment group.
Results: Flow cytometry analysis showed that the relative number of NF-κB+ cells increased (18.38±1.24%) in LPS-induced mice compared with normal mice (13.24±1.15%). The Sarcophyton spp. DCM extracts decreased the relative number of NF-κB+ cells (125 mg/kg: 13.96±0.84%). Immunohistochemical analysis with ImmunoMembrane showed that LPS induction in mice increased iNOS expression when compared to normal mice. The Sarcophyton spp. DCM extracts reduced iNOS expression (especially at 125 mg/kg).
Conclusion: DCM extracts of Sarcophyton spp. inhibited the activation of NF-κB, resulting in suppressed iNOS expression, which directly inhibits NO production.
Keywords: Alcyoniidae, anti-inflammatory, lipopolysaccharide, nitric oxide, soft coral.

Monday, 2 December 2019

Diversity and prevalence of gastrointestinal parasites in farmed pigs in Southeast Gabon, Central Africa

Research (Published online: 02-12-2019)
1. Diversity and prevalence of gastrointestinal parasites in farmed pigs in Southeast Gabon, Central Africa
Gael Darren Maganga, Linda Bohou Kombila, Larson Boundenga, Ivan Cyr Moussadji Kinga, Judicael Obame-Nkoghe, Herve Tchoffo, Oubri Bassa Gbati and Julius Awah-Ndukum
Veterinary World, 12(12): 1888-1896
ABSTRACT
Background and Aim: Gastrointestinal infestations caused by intestinal parasites are the most important diseases and the most common in pigs in the tropics. These parasites are often associated with a huge economic loss. This study aimed to assess the diversity and prevalence of gastrointestinal parasites in farmed pigs from Haut-Ogooue Province, in South East Gabon.
Materials and Methods: From March 2018 to July 2018, 156 samples of pig feces collected from nine different farms were analyzed under light microscopy. The identification of eggs, cysts, and oocysts in fecal samples was done using two qualitative techniques: Flotation and sedimentation.
Results: After examination, the results obtained revealed an overall infestation level of 98.7% (154/156). We found ten parasite types with infestation levels that varied from species: Balantidium coli (120/156), Oesophagostomum spp. (100/156), Isospora suis (102/156), Ancylostoma spp. (17/156), Trichostrongylus spp. (28/156), Hyostrongylus spp. (13/156), Strongyloides spp. (7/156), Ascaris suum (8/156), Globocephalus spp. (1/156), and spirurida (1/156). The study of risk factors revealed that factors such as sex, age, and physiological condition may influence the diversity and level of infestation of animals by gastrointestinal parasites.
Conclusion: For better prevention of parasitism in these farms, it would be interesting to implement health monitoring and to ensure good hygiene. Finally, further studies would be needed to better evaluate the distribution of these parasites in Gabon and the involvement of these animals in the transmission cycle of parasitic zoonoses.
Keywords: diversity, Gabon, gastrointestinal parasites, pigs, prevalence.