Occurrence, characterization, and antibiogram of Staphylococcus aureus in meat, meat products, and some seafood from Libyan retail markets

Research (Published online: 29-06-2019)

30. Occurrence, characterization, and antibiogram of Staphylococcus aureus in meat, meat products, and some seafood from Libyan retail markets

Hesham T. Naas, Ramadan A. Edarhoby, Aboubaker M. Garbaj, Salah M. Azwai, Said K. Abolghait, Fatim T. Gammoudi, Ashraf A. Moawad, Ilaria Barbieri and Ibrahim M. Eldaghayes

Veterinary World, 12(6): 925-931

ABSTRACT

Aim: The aim of the current investigation was to screen the presence of Staphylococci spp., especially S. aureus in meat, meat products of different animal species, and some seafood sold in some retail markets in Libya using cultural and molecular techniques, and to study their antibiotics resistance profiles.

Materials and Methods: A total of 139 samples from red meat, meat products, and seafood were collected from many areas in Libya. Enumeration and isolation of Staphylococci spp. and S. aureus by normal cultural methods followed by molecular identification using molecular techniques by bacterial DNA extraction and partial sequencing of 16S rDNA.

Results: Out of 139 samples, 112 (80.6%) were contaminated with different species of Staphylococci based on cultural characteristics of Staphylococci on Baird-Parker medium, for which S. aureus was detected in only 32 samples (23%). However, only six out of 18 (33.3%) isolates sent for sequencing were confirmed to be S. aureus using the molecular technique. The six identified isolates of S. aureus were tested for antimicrobial resistance against 24 most commonly used antibiotics. All isolates were resistant to only two antibiotics (cefotaxime and clindamycin). Among these six isolates, only one confirmed to be Methicillin-resistant Staphylococcus aureus.

Conclusion: Results of this study suggest that food of animal origin could be a source of S. aureus with antimicrobial resistance characteristics that can be spread through the food chain, and raise the importance of these results for public health.

Keywords: 16S rDNA, antibiogram, meat products, meat, seafood, Staphylococcus aureus.

Comparison of biotechnological culture of hypoxia-conditioned rat mesenchymal stem cells with conventional in vitro culture of normoxia-conditioned rat mesenchymal stem cells for testicular failure therapy with low libido in rats

Research (Published online: 28-06-2019)

29. Comparison of biotechnological culture of hypoxia-conditioned rat mesenchymal stem cells with conventional in vitro culture of normoxia-conditioned rat mesenchymal stem cells for testicular failure therapy with low libido in rats

Erma Safitri and Mas'ud Hariadi

Veterinary World, 12(6): 916-924

ABSTRACT

Aim: Biotechnological culture of hypoxia-conditioned (CH) rat mesenchymal stem cells (rMSC-CH) for testicular failure therapy with low libido improves the functional outcome of the testicle for producing spermatogenic cells and repairs Leydig cells in rats (Rattus norvegicus).

Materials and Methods: In the first group (T1), rats with testicular failure and low libido were injected with normoxia-conditioned (CN) rMSCs (21% oxygen); in the second group (T2), rats with testicular failure and low libido were injected with rMSC-CH (1% oxygen); in the negative control group (T–), rats with normal testis were injected with 0.1 mL phosphate-buffered saline (PBS); and in the sham group (TS), rats with testicular failure and low libido were injected with 0.1 mL of PBS.

Results: Vascular endothelial growth factor expression, as the homing signal, in the groups T2, T–, T1, and TS was 2.00±0.5%, 2.95±0.4%, 0.33±0.48%, and 0±0%, respectively. The number of cluster of differentiation (CD)34+ and CD45+ cells in the groups T– and TS was <20%, whereas that in T1 and T2 groups was >30% and >80%, respectively, showing the mobilization of hematopoietic stem cells (HSCs). The number of spermatogenic cells (spermatogonia, primary spermatocytes, secondary spermatocytes, and spermatid) decreased significantly (p<0.05) in TS compared with that in T–, T1, and T2, whereas that in T2 did not show a significant (p>0.05) decrease compared to that in T–. The improvement in libido, based on the number of Leydig cells producing the hormone testosterone for libido expression, did not increase in T1, whereas T2 was able to maintain the number of Leydig cells significantly compared to that between TS and T1.

Conclusion: rMSC-CH culture for testicular failure with low libido showed improvement in the functional outcome of the testicle and in repairing Leydig cells.

Keywords: hypoxia-conditioned rat mesenchymal stem cells, low libido, normoxia-conditioned rat mesenchymal stem cells, rat, testicular failure.

Prevalence and molecular characterization of infectious bronchitis virus isolated from chicken in Bangladesh

Research (Published online: 28-06-2019)

28. Prevalence and molecular characterization of infectious bronchitis virus isolated from chicken in Bangladesh

Zafar Ahmed Bhuiyan, Md. Zulfekar Ali, Mohammad Moktader Moula, Md. Giasuddin and Zahed Uddin Mahmood Khan

Veterinary World, 12(6): 909-915

ABSTRACT

Aim: The present study was aimed to determine the prevalence of infectious bronchitis virus (IBV) as well as virus isolation, identification, and molecular characterization of various strains circulating in Bangladesh.

Materials and Methods: A total of 371 swabs and organ samples were collected from four types of chicken including layer, Sonali (local), broiler, and broiler breeder under eight districts (Rangpur, Bogura, Tangail, Dhaka, Gazipur, Mymensingh, Jamalpur, and Cumilla) during 2014-2016 in Bangladesh.

Results: Out of 371 samples, 65 samples were positive in reverse transcriptase polymerase chain reaction (RT-PCR) for molecular identification of IBV. The overall prevalence was 17.52% recorded and among the selected types of chicken, the highest prevalence of IBV was found in layer that was 42.22% followed by 17.24% in Sonali, 14.93% in broiler breeder, and lowest prevalence was 11.94% in broiler chicken, respectively. Moreover, the prevalence of IBV was recorded highest in aged chicken at 41-60 weeks, which was 54.55% in layer, 27.27% in Sonali, and, afterward, 14.68% was found in broiler breeder, respectively. Frequency of IBV more frequently in winter (22.67%) followed by rainy (15.87%) and summer season (11.58%). The highest prevalence of IBV was found Tangail district (41.67%) followed by Mymensingh (24.42%), Gazipur (19.32%), Dhaka (15.38%), Jamalpur (16.67%), Bogura (13.68%), Cumilla (5.88%), and Rangpur (9.26%), respectively. Samples that were found high positive in IBV RT-PCR (Ct value below 30) were subjected to inoculation into chicken egg embryo to observe characteristic changes in chicken embryo. Swabs and organ samples were processed and passaged in 9-day-old embryonated chicken eggs through allantoic cavity route. IBV virus suspected samples inoculated into chicken egg embryos after 3-5 passages showed dwarfing and curling of the embryos which are characteristic lesions of IBV. Allantoic fluid was collected from all inoculated eggs and performed partial sequencing of S1 gene for three isolates. After sequencing, the phylogenetic tree was constructed from the nucleotide sequences of IBV isolates. Two of the isolates are 4/91 IBV and another one matched with QX-like IBV.

Conclusion: The results revealed that the three isolates from different places in Bangladesh were identified for the 1st time as which will help for IBV control strategy.

Keywords: chicken, infectious bronchitis virus, isolation, molecular, prevalence.

Comparative study between the excision-ligation and autoligation of vas deferens technique for teaser rams preparation

Research (Published online: 27-06-2019)

27. Comparative study between the excision-ligation and autoligation of vas deferens technique for teaser rams preparation

Dhurgham Hameed Al Haideri, Hussein Kareem Ibraheim and Falah Baiee

Veterinary World, 12(6): 901-908

ABSTRACT

Aim: The present study was designed to demonstrate the autoligation (AL) of vas deferens and the excision-ligation (EL) technique to generate vasectomized rams to reduce the complications, operative time, and price of the vasectomy techniques.

Materials and Methods: A total of 12 healthy and mature Iraqi Awassi rams were used, which divided into two groups, six rams for each one. The former group was performed the EL technique while the latter group, the AL of vas deferens technique was used.

Results: The results of the present study found that both techniques were same with the reproductive efficient examinations that mean the two techniques had same ability to close the male genital passage for teaser rams preparation. However, the methods were different with the histopathological changes, operation time, prices, and complications, which were minor in the AL of vas deferens compared with the EL technique.

Conclusion: The AL technique of vas deferens to prepare teaser animal is recommended over the EL technique due to different aspects such as cost, fewer complications, and active teaser for a long period are the main aspects of AL technique.

Keywords: ram infertility, testes, vas occlusion, vasectomy techniques.

Genetic variation and phylogenetic analysis of Indonesian indigenous catfish based on mitochondrial cytochrome oxidase subunit III gene

Research (Published online: 27-06-2019)

26. Genetic variation and phylogenetic analysis of Indonesian indigenous catfish based on mitochondrial cytochrome oxidase subunit III gene

Rini Widayanti, Aris Haryanto, Wayan Tunas Artama and Suhendra Pakpahan

Veterinary World, 12(6): 896-900

ABSTRACT

Aim: This study aimed to analyze the genetic variation and phylogenetic reconstruction of Indonesian indigenous catfish using mitochondrial cytochrome oxidase subunit III sequences.

Materials and Methods: A total of 19 samples of catfish were collected from seven rivers (Elo [EM], Progo [PM], Kampar [KR], Musi [MP], Mahakam [MS], Kapuas [KS], and Bengawan Solo [BSBJ]) in five different geographical locations in Indonesia. The genome was isolated from the tissue. Mitochondrial DNA cytochrome oxidase subunit III was amplified using polymerase chain reaction (PCR) with CO3F and CO3R primers. The PCR products were sequenced and continued to analyze genetic variation and phylogenetic relationship using MEGA version 7.0 software.

Results: Cytochrome c oxidase (COX)-III gene sequencing obtained 784 nucleotides encoding 261 amino acids. Sequenced COX-III gene fragments were aligned along with other catfish from Genbank using ClustalW program and genetic diversity among species was analyzed using the MEGA Version 7.0 software. Among all samples, there were substitution mutations at 78 nucleotide sites, and there were 14 variations in amino acids. Catfish from PM, KR, MP, and KS had the same amino acids as Hemibagrus nemurus (KJ573466.1), while EM catfish had eight different amino acids and catfish BSBJhad 12 different amino acids.

Conclusion: Indonesian catfish divided into four clades. BBSJ Catfish were grouped with Pangasianodon gigas, EM catfish were grouped with Mystus rhegma, and KS catfish were grouped with Hemibagrus spilopterus, while catfish MS, KR, PM, and MP were grouped with H. nemurus.

Keywords: cytochrome c oxidase-III, Hemibagrus, Indonesian indigenous catfish, mitochondrial DNA, phylogenetic.

Effects of rations containing formaldehyde-protected soybean meal on meat production in Kacang goats

Research (Published online: 25-06-2019)

25. Effects of rations containing formaldehyde-protected soybean meal on meat production in Kacang goats

Retno Adiwinarti, I. Gede Suparta Budisatria, K. Kustantinah, R. Rusman and Edwin Indarto

Veterinary World, 12(6): 890-895

ABSTRACT

Aim: This study aimed to investigate effects of rations containing formaldehyde-protected soybean meal on meat production in Kacang goats.

Materials and Methods: Fourteen yearling Kacang bucks, weighing 15.8-19.8 kg, were arranged in a completely randomized design. The treatments included a control (PSBM0): 100% untreated SBM; PSBM50: 50% untreated SBM + 50% formaldehyde-protected SBM; and PSBM100: 100% formaldehyde-protected SBM.

Results: The goats disliked the protected SBM. Therefore, differences in their intakes were reflected in their average daily gain (ADG). The ADG and slaughtered weight of the control group were the highest, while those of the PSBM100 and PSBM50 groups were similar. The carcass weights and meat production of the control group were higher than those of the PSBM50 group, but the retained protein to the meat conversion ratio of the PSBM50 group was lower than that of the control. The carcass percentages were similar between the treatments.

Conclusion: The retained protein to meat conversion ratio of Kacang goats fed with 50% formaldehyde-protected SBM showed the lowest value, indicating that these rations efficiently produced meat in the carcass.

Keywords: carcass, chevon quality, daily gain, Kacang goat, soybean meal.

A sensitive serodiagnostic tool for the detection of active infection of zoonotic visceral and nasopharyngeal linguatulosis

Research (Published online: 24-06-2019)

24. A sensitive serodiagnostic tool for the detection of active infection of zoonotic visceral and nasopharyngeal linguatulosis

Marwa M. Attia, Elshaimaa Ismael and Nagla M. K. Saleh

Veterinary World, 12(6): 883-889

ABSTRACT

Aim: This study aimed to evaluate the different serological techniques for early diagnosis of acute concurrent infections with linguatulosis in the definitive host (dogs) and an intermediate host (goats). This evaluation compared between the gold standard (GS) test (GS; examination of nasal and fecal samples in dogs and examination of lymph nodes in goats), sandwich enzyme-linked immunosorbent assay (S-ELISA), and indirect ELISA.

Materials and Methods: Fifty goats and fifty dogs were examined for the presence of Linguatula serrata nymphs and adults, respectively, besides the collection of blood samples from the examined animals for serologic testing.

Results: In goats; GS, S-ELISA, and indirect ELISA showed positivity in 32 (64%), 28 (56%), and 39 (78%) samples, respectively. In dogs; GS, S-ELISA, and indirect ELISA showed positivity in 25 (50%), 25 (50%), and 30 (60%) samples, respectively. S-ELISA displayed significant higher agreement with the GS test (≥0.83) than indirect ELISA (≤0.67) in both hosts. Infection with linguatulosis showed significant relation with the age of goats and dogs and the sex of goats (p<0.05).

Conclusion: S-ELISA displayed more sensitivity and specificity for the detection of concurrent infections with linguatulosis in both dogs and goats than indirect ELISA, which could detect the prior infections. Similarly, these assays could be used for diagnosis of concurrent infections with linguatulosis in human, especially the chronic ones.

Keywords: halzoun, indirect enzyme-linked immunosorbent assay, Linguatula serrata, sandwich enzyme-linked immunosorbent assay, visceral linguatulosis.