Diversity of lactic acid bacteria in dadih produced by either back-slopping or spontaneous fermentation from two different regions of West Sumatra, Indonesia

Research (Published online: 15-06-2019)

15. Diversity of lactic acid bacteria in dadih produced by either back-slopping or spontaneous fermentation from two different regions of West Sumatra, Indonesia

Chandra Utami Wirawati, Mirnawati Bachrum Sudarwanto, Denny Widaya Lukman, Ietje Wientarsih and Eko Agus Srihanto

Veterinary World, 12(6): 823-829

ABSTRACT

Aim: Dadih samples from two different origins (Kamang and Gadut in West Sumatra) manufactured with different methods (back-slopping or spontaneous fermentation) were evaluated for the diversity of lactic acid bacteria (LAB).

Materials and Methods: Four dadih samples manufactured with two different fermentation methods were obtained from Kamang and Gadut regions. Both genotypic and phenotypic characteristic (16S rRNA partial gene sequence analysis and carbohydrate fermentation profile) were used to analyze the diversity of dadih LAB population.

Results: This study showed that LAB count in back-slopping fermented dadih was one log cycle higher than spontaneous fermented dadih. LAB isolates from the two regions were divided into three genera, namely Lactococcus, Lactobacillus, and Pediococcus. Sequencing results showed that 41.6% (five isolates) were identified as Lactococcus lactis ssp. lactis, 25% (three isolates) were identified as Lactobacillus plantarum ssp. plantarum, 16.6% (two isolates) were identified as L. lactis ssp. cremoris, and 8.3% (one isolate each) were identified as Pediococcus pentosaceus and Lactobacillus pentosus.

Conclusion: Five species were determined in back-slopping fermented dadih, i.e., L. lactis ssp. lactis, L. lactis ssp. cremoris, L. plantarum ssp. plantarum, L. pentosus, and P. pentosaceus. On the other hand, spontaneous fermented dadih only contained three different species, namely L. lactis ssp. lactis, L. lactis ssp. cremoris, and L. plantarum ssp. plantarum. This research showed that back-slopping fermentation offers greater abundance and diversity compared to spontaneous fermentation in dadih.

Keywords: back-slopping, dadih, lactic acid bacteria, spontaneous fermentation.

Phenotypic parameters affecting reproduction and production performances of dairy cattle in peri-urban of Bamako, Mali

Research (Published online: 14-06-2019)

14. Phenotypic parameters affecting reproduction and production performances of dairy cattle in peri-urban of Bamako, Mali

Abdoulaye Toure, Nicolas Antoine-Moussiaux, Fikremariam Geda, Ali Kouriba, Diakaridia Traore, Bakary Traore, Pascal Leroy and Nassim Moula

Veterinary World, 12(6): 817-822

ABSTRACT

Aim: The present study was conducted to evaluate the reproduction and production performances of crossbred and local dairy cattle in peri-urban of Bamako, the capital of Mali.

Materials and Methods: A total of 17 randomly selected households with 450 dairy cattle from four peri-urban of Bamako were individually interviewed, given register format and divided into four focus group discussions. The participants were dairy farmers and they were asked to know the phenotype that seems to them the more interesting for the reproduction and production performances of their dairy cattle.

Results: The calving interval (CI) obtained exceeded 433 days in all phenotypic types with average milk yields of 5.13±1.84 kg/day, 4.76±2.41, and 3.05±1.32 kg/day, respectively, for the Holstein crossbred, Montbeliard crossbred, and the local breeds (Zebu Peul and/or Zebu Maure). Crossbred was more productive than local breeds with significant (p<0.05) differences for lactation length, CI and total production and not significant on parity. The results also showed the advantages of the crossbred cows in terms of CI (460±80 days) compared to local zebus breeds (433±115 days) to keep the time of milking as long as possible. However, the Zebu Azawak breed whose cradle is located in Northern Mali, managed under extensive peri-urban rearing conditions, has less favorable production parameters than those of other local cattle breeds in milk production (636±43.3 kg vs. 681±41.1 kg).

Conclusion: The present study revealed that crossbred dairy cattle performed better in terms of CI, lactation length, and production compared to the local breeds. The study also showed that the local Azawak breed numerically performed less compared to the other local breeds evaluated in this study, namely, Peul and Maure.

Keywords: cattle, Mali, milk production, peri-urban, reproduction.

In vivo approach on femur bone regeneration of white rat (Rattus norvegicus) with the use of hydroxyapatite from cuttlefish bone (Sepia spp.) as bone filler

Research (Published online: 14-06-2019)

13. In vivo approach on femur bone regeneration of white rat (Rattus norvegicus) with the use of hydroxyapatite from cuttlefish bone (Sepia spp.) as bone filler

Aminatun Aminatun, Fadhilah D.E. Handayani, Prihartini Widiyanti, Dwi Winarni and Siswanto Siswanto

Veterinary World, 12(6): 809-816

ABSTRACT

Background: Hydroxyapatite (HA) from bovine bone has been widely used as bone filler in many fractures cases. HA can also be made from cuttlefish bone (Sepia spp.) that has abundant availability in Indonesia and contains 84% CaCO3, which is a basic ingredient of HA. However, research on the effects of HA from cuttlefish bone on bone regeneration parameters has not been done yet.

Aim: This study aimed to determine femur bone regeneration of white rats (Rattus norvegicus) through the use of HA from cuttlefish bone (Sepia spp.) as bone filler.

Materials and Methods: HA was made using the hydrothermal method by mixing 1M aragonite (CaCO3) from cuttlefish bone and 0.6 M NH4H2PO4 at 200°C for 12 h followed by sintering at 900°C for 1 h. In vivo test was carried out in three groups, including control group, bovine bone-derived HA group, and cuttlefish bone-derived HA group. The generation of femur bone was observed through the number of osteoblasts, osteoclasts, woven bone, lamellar bone, havers system, and repair bone through anatomical pathology test for 28 days and 56 days.

Results: Anatomical pathology test results are showed that administration of bovine bone-derived HA and cuttlefish bone-derived HA increased the number of osteoblasts, osteoclasts, woven bone, lamellar bone, havers system, and bone repair at recuperation of 56 days. Statistical test using Statistical Package for the Social Sciences with Kruskal–Wallis and Mann–Whitney U-test was resulted in significant differences between the bovine bone-derived HA control group and the cuttlefish-derived HA control group. There was no significant difference toward the indication of bone formation through the growth of osteoblasts, osteoclasts, woven bone, lamellar bone, havers system, and bone repair in the bovine bone-derived HA and cuttlefish bone-derived HA groups.

Conclusion: It can be concluded that cuttlefish bone-derived HA has the potential as bone filler based on the characteristics of bone regeneration through in vivo test.

Keywords: anatomical pathology, bone filler, cuttlefish bone (Sepia spp.), hydrothermal, hydroxyapatite.

Antibacterial efficacy of Moringa oleifera leaf extract against pyogenic bacteria isolated from a dromedary camel (Camelus dromedarius) abscess

Research (Published online: 14-06-2019)

12. Antibacterial efficacy of Moringa oleifera leaf extract against pyogenic bacteria isolated from a dromedary camel (Camelus dromedarius) abscess

Ehab Ali Fouad, Azza S. M. Abu Elnaga and Mai M. Kandil

Veterinary World, 12(6): 802-808

ABSTRACT

Background: Abscess in camel is one of the most important bacterial infections. It causes anemia and emaciation, resulting in an economic loss due to carcass condemnation and a decrease in reproductive and production efficiency.

Aim: This investigation aimed to isolate the bacteria from abscesses in camels and evaluate the antibacterial activity of Moringa oleifera extracts.

Materials and Methods: Disk diffusion method and minimum inhibitory concentration were used for the evaluation of the antibacterial activity of M. oleifera extracts against isolated bacteria from camel abscesses.

Results: The isolated bacteria were displayed relatively as follows: Corynebacterium pseudotuberculosis (30.4%), Staphylococcus aureus (25.8%), Escherichia coli (17.8%), Corynebacterium ulcerans (10.5%), Klebsiella pneumoniae (8.5%), Pseudomonas aeruginosa (8.5%), Micrococcus spp. (6.7%), Proteus vulgaris (5.2%), Citrobacter spp. (4.2%), and Staphylococcus epidermidis (1.7%). The drugs of choice for Corynebacterium isolates were ciprofloxacin and trimethoprim/ sulfamethoxazole, whereas amikacin, ciprofloxacin, gentamicin, neomycin, novobiocin, streptomycin, and vancomycin were for Staphylococcus isolates. Moreover, the ethanol extracts of M. oleifera showed higher antibacterial efficacy than the cold aqueous extracts.

Conclusion: M. oleifera is considered one of the new infection-fighting strategies in controlling pyogenic bacteria responsible for camel abscesses.

Keywords: antibacterial activity, camel abscess, Moringa oleifera, pyogenic bacteria.

Effect of yeast cell wall supplementation on production performances and blood biochemical indices of dairy cows in different lactation periods

Research (Published online: 13-06-2019)

11. Effect of yeast cell wall supplementation on production performances and blood biochemical indices of dairy cows in different lactation periods

Min Aung, Hiromichi Ohtsuka and Kenichi Izumi

Veterinary World, 12(6): 796-801

ABSTRACT

Aim: This experiment was conducted to determine the effect of yeast cell wall (YCW) supplementation on production performances and blood biochemical indices such as liver enzyme activities, energy metabolites, and electrolyte concentrations of dairy cows in different lactation periods (LP).

Materials and Methods: Thirty-two lactating Holstein cows were assigned into 2×2 factorial arrangement, in which the factors were the treatment (TM) (control [n=16] vs. YCW [n=16]) and the LP (early lactation [n=14] vs. mid-lactation [n=18]). The cows with day in milk (DIM) <120 (81±7 DIM) were defined as early lactating cows, whereas the cows with DIM >120 (179±5 DIM) were assumed as mid-lactating cows. The YCW (SafMannan; Phileo, Lesaffre Animal Care, France) was used as the dietary supplement (10 g/cow/day) in this experiment. The statistical analysis of the data was performed by the two-way analysis of variance using the general linear model procedure to determine the main effects (TM and LP) and their interaction (TM×LP) on production performances and blood biochemical parameters of experimental cows.

Results: No significant effects (p>0.05) of YCW supplementation on production performances and blood biochemical indices of cows in TM groups (control vs. YCW) were observed; however, some obvious effects were detected in LP (early- and mid-lactation). Milk and milk component yield of cows in early lactation were significantly higher (p<0.05) than in mid-lactation, whereas somatic cell count and milk urea nitrogen were not different (p>0.05) with the YCW supplementation. The higher level (p<0.05) of serum albumin was found in mid-lactating cows after YCW supplementation. Before the experiment, the higher (p<0.05) non-esterified fatty acid (NEFA) and NEFA/total cholesterol (T-Cho) ratio, and the lower (p<0.05) calcium (Ca) concentration were observed in early lactating cows comparison with mid-lactating cows; however, there were not different after YCW supplementation.

Conclusion: The positive effects of YCW supplementation on milk and milk component yields, energy metabolite, especially NEFA and NEFA/T-Cho ratio and Ca concentration were observed in early lactating cows rather than mid-lactating cows.

Keywords: dairy cows, electrolyte indices, energy metabolites, liver enzyme activity, milk yield, yeast cell wall.

Clinical and ultrasonographic investigations of 30 water buffaloes (Bubalus bubalis) with hepatomegaly

Research (Published online: 12-06-2019)

10. Clinical and ultrasonographic investigations of 30 water buffaloes (Bubalus bubalis) with hepatomegaly

A. M. Abdelaal, M. Abd El Raouf, M. A. Aref and A. A. Moselhy

Veterinary World, 12(6): 789-795

ABSTRACT

Background and Aim: Knowledge of normal ultrasonographic dimensions of the liver and associated vascular structures is an important indicator for the diagnosis of hepatic diseases. Enlargement of the liver beyond its normal dimensions is the term of hepatomegaly and ultrasonography is the primary and the suitable diagnostic technique for this condition. Therefore, this study aimed to describe the clinical and ultrasonographic findings of liver diseases causing hepatomegaly in 30 buffaloes as well as to provide a range of liver dimensions and its blood vessel measurements in normal and diseased buffaloes.

Materials and Methods: The study population included 30 buffaloes that were admitted to the clinic of the Faculty of Veterinary Medicine - Zagazig University for investigation of clinical signs associated with gastrointestinal diseases such as anorexia, chronic weight loss, and variable degrees of diarrhea or constipation. The animals were subjected to thorough clinical and ultrasonographic investigations. In addition, 10 healthy buffaloes were investigated ultrasonographically and post-slaughtering for comparison of liver dimensions and physical appearance.

Results: Three conditions causing hepatomegaly were identified in this study as multiple focal hepatic lesions, diffuse fatty liver, and hepatic congestion. Clinically, it was difficult to differentiate between each condition while ultrasonography was the ideal tool for diagnosis after comparing with necropsy as a gold standard tool. Hepatomegaly was recorded in all affected animals with a significant decrease in the size of the portal vein (PV) and caudal vena cava (CVC) in animals affected with multiple focal hepatic lesions and fatty liver disease while the size of the PV and CVC was significantly increased in buffaloes with hepatic congestion.

Conclusion: Ultrasonography can aid to accurately identify buffaloes with hepatomegaly and differentiate between different lesions involved.

Keywords: buffaloes, fatty liver, hepatic congestion, hepatomegaly, ultrasonography.

Growth performance and meat characteristics of the first filial Awassi Rambouillet callipyge ram lambs

Research (Published online: 11-06-2019)

9. Growth performance and meat characteristics of the first filial Awassi Rambouillet callipyge ram lambs

Khaleel I. Z. Jawasreh, A. H. Al-Amareen and P. Y. Aad

Veterinary World, 12(6): 783-788

ABSTRACT

Aim: This study was designed to introduce the callipyge (CLPG) and 50% of Rambouillet sheep genes to improve meat quality and quantity of Awassi (AW) sheep.

Materials and Methods: The CLPG mutation was introduced into the AW sheep through frozen semen of homozygous Rambouillet rams for the CLPG mutation. Four ram lambs from the first-generation Rambouillet callipyge Awassi (F1-RCA) and five from pure AW were recruited for a fattening trial conducted in individual pens using standard ration, following which ram lambs were slaughtered for carcass and meat evaluation.

Results: Final body weight, dry matter intake, average daily gain, and feed conversion ratio were significantly higher in F1-RCA than AW. Hot and cold carcass weights and the other carcass cuts' weights, except for the fat tail, were heavier in F1-RCA than AW. There was no difference in dressing percentage between the two genetic groups (p>0.05). All non-carcass components' weights, except spleen, kidney, and testis, were higher in F1-RCA. Total lean, total bone, and intermuscular fat weight were greater in F1-RCA, but bone-to-lean ratio was lower in F1-RCA when compared with AW (p<0.01). No differences (p>0.05) were observed in all meat quality parameters for muscle longissimus with the exception of pH, redness color, and tenderness that were lower (p<0.05) in F1-RCA than AW. F1-RCA lambs had larger longissimus muscle area (30.9 vs. 16.9 cm2) and less leg fat depth (11.1 vs. 17.4 mm).

Conclusion: The implications of this research show the potential of 50% of Rambouillet genes and the CLPG mutation to improve growth and meat characteristics in AW-Rambouillet crosses and can be used further to develop a meat-type AW with improved productivity and muscle mass.

Keywords: Awassi sheep, callipyge, carcass composition, gene introgression, growth performance.