Epidemiological aspects of cutaneous leishmaniasis in Southwest of Iran

Research (Published online: 03-06-2019)

7. Epidemiological aspects of cutaneous leishmaniasis in Southwest of Iran

Mohammad Darvishi, Abedin Saghafipour, Eslam Moradi-Asl, Reza Jafari, Seyed Jafar Adnani Sadati and Hossein Darabi

International Journal of One Health, 5: 49-53

ABSTRACT

Background and Aim: Zoonotic rodents (Rodentia: Muridae: Gerbillinae) and Phlebotominae sand flies are the major reservoir hosts and vectors of Leishmania major causing zoonotic cutaneous leishmaniasis, respectively. This study was carried out to evaluate the epidemiological aspects of cutaneous leishmaniasis (CL) in Tangestan County, Southwest Iran.

Materials and Methods: This descriptive cross-sectional study was carried out on human cases, probable reservoirs, and vectors of CL at Tangestan County in 2017. The data of patients were taken on health centers and analyzed. To determination faunistic of sand flies, they were collected using sticky paper traps and identified using identification keys. The rodent samples were captured using Sherman live traps and determined for amastigote infection.

Results: A total of 436 cases occurred during 2005-2017 and the incidence rate of disease was 0.2-12/100,000 people. Six species of sand flies including three species of Phlebotomus and three species of Sergentomyiaincluding Phlebotomus papatasi, Phlebotomus sergenti, Phlebotomus alexandri, Sergentomyia tiberiadis, Sergentomyia baghdadis, and Sergentomyia clydei were identified. From 185 trapped rodents, 9 species were identified. We founded, 2 out of 132 Tatera indica (1.53%) were positive with Leishmania spp. using the microscopic method.

Conclusion: According to the existence of human leishmaniasis cases as a host, T. indica with high abundance and infected to Leishmania as a reservoir, and existence of P. papatasi with high abundance as a suspected vector of disease; there is a potential for transmission of disease in the area. Design of exact control and prevention strategies is highly recommended.

Keywords: cutaneous leishmaniasis, Iran, rodents, sand flies.

Echocardiographic study of healthy Indian Spitz dogs with normal reference ranges for the breed

Research (Published online: 01-06-2019)

2. Echocardiographic study of healthy Indian Spitz dogs with normal reference ranges for the breed

Deepti Bodh, Mozammel Hoque and Abhishek Chandra Saxena

Veterinary World, 12(5): 740-747

ABSTRACT

Aim: The present study was aimed to determine the normal reference values of M-mode echocardiographic measurements in healthy Indian Spitz dogs and evaluate the influence of gender and body weight on these measurements.

Materials and Methods: M-mode echocardiography was performed in twenty-four clinically healthy conscious Indian Spitz dogs, aged 3-5 years and weighing 7-18 kg. Measurements were made from the right parasternal long axis left ventricular outflow tract view of the heart. The parameters recorded were: Left ventricular internal dimension, interventricular septal thickness and left ventricular posterior wall thickness during diastole and systole, left atrial diameter, aortic root diameter, left ventricular systolic functional parameters, and indices and mitral valve parameters.

Results: M-mode echocardiographic measurements in healthy Indian Spitz dogs were standardized. Gender had no influence on echocardiographic measurements except mitral valve excursion amplitude and time interval between onset and end of mitral valve closure, which were significantly (p<0.05) higher in females than males. Left ventricular internal dimension at end-diastole, left ventricular internal dimension at end-systole, left ventricular posterior wall dimension at end-systole, end-diastolic volume, end-systolic volume, stroke volume, cardiac output, left ventricular ejection time, and mitral valve excursion amplitude correlated significantly (p<0.05) with body weight in Indian Spitz dogs.

Conclusion: Data obtained in the present study can be used as breed-specific reference values for cardiac disease diagnosis as well as for future studies in Indian Spitz dogs.

Keywords: dogs, echocardiography, Indian Spitz, M-mode.

Immunomodulatory potential of polysaccharides from Coriolus versicolor against intracellular bacteria Neisseria gonorrhoeae

Research (Published online: 01-06-2019)

1. Immunomodulatory potential of polysaccharides from Coriolus versicolor against intracellular bacteria Neisseria gonorrhoeae

Manikya Pramudya and Sri Puji Astuti Wahyuningsih

Veterinary World, 12(5): 735-739

ABSTRACT

Background and Aim: For many years, people use natural products from the plant and fungal to improve immune response against microorganism. This study aimed to investigate the immunomodulatory properties of polysaccharides (PS) from Coriolus versicolor in mice infected by intracellular bacteria Neisseria gonorrhoeae.

Materials and Methods: Thirty-six female BALB/C mice were divided into six groups: Normal control, negative control, positive control, P1 (PS before infection), P2 (PS after infection), and P3 (PS before and after infection). PS were administrated for 10 days. N. gonorrhoeae was infected twice with 2 weeks gap from the first to second exposure with a dose of 106 cells. 1 week after the end of treatment, level of oxidants, innate immune responses, and adaptive immune responses were measured.

Results: This study showed that PS administration could restore the number of leukocytes as normal but could not enhance the number of phagocytes and its activity. PS administration also showed immunosuppression activity by lowering nitric oxide levels in P2 and P3 groups (p<0.05). This result showed that PS prevent over-expression of pro-inflammatory cytokines by decreasing phagocytic activity. Contrast with innate immune response result; PS administration could significantly increase interferon-gamma level in P1, P2, and P3 groups (p<0.05). Level of antibodies was significantly increased in the P3 group (p<0.05). PS administration also showed an increased level of tumor necrosis factor-a, but the difference was not significant (p>0.05).

Conclusion: PS enhance adaptive immunity due to the capability of N. gonorrhoeae that able to survive and replicate in phagocytes. Thus, PS from C. versicolor could be potentially be used as a natural immunomodulator against intracellular bacteria.

Keywords: immune response, immunomodulator, Neisseria gonorrhoeae, polysaccharide.

Prevalence and risk factors associated with Cryptosporidium spp. infection in local breed of dogs in Enugu State, Nigeria

Research (Published online: 31-05-2019)

17. Prevalence and risk factors associated with Cryptosporidium spp. infection in local breed of dogs in Enugu State, Nigeria

Ukamaka U. Eze, Ikenna O. Ezeh, Terry A. Nzeakor, Samuel C. Attama, Ekene V. Ezenduka and Denchris N. Onah

Veterinary World, 12(5): 729-734

ABSTRACT

Aims: Cryptosporidiosis is an important zoonotic disease of major public and veterinary concern. The disease affects humans and a variety of animal species including the domestic dog. This study aimed to determine the prevalence and risk factors associated with Cryptosporidium spp. infection in local breed of dogs from different homes and those presented at veterinary hospitals and clinics in Enugu State, Nigeria.

Materials and Methods: A total of 203 fresh fecal samples were collected from domestic dogs in six local government areas in Enugu State from February 2015 to August 2015. All the samples were examined using the formol-ether sedimentation method. Fecal smears were then stained by the modified Ziehl-Neelsen technique and examined under direct light microscopy.

Results: A total of 74 (36.5%) dogs were infected with Cryptosporidium spp. oocysts. There was a strong association (p<0.05) between the presence of Cryptosporidium spp. oocysts and management practices. However, there was no statistically significant association (p>0.05) between the presence of Cryptosporidium spp. oocysts and age, sex, and fecal consistency.

Conclusion: The findings of this work suggest that domestic dogs in Enugu State harbor and shed Cryptosporidium spp. oocysts in the environment, especially those managed semi-intensively. Such fecal shedding is particularly so and of greater zoonotic and epidemiological importance in animals that do not show clinical signs and therefore not treated. They, therefore, pose a greater public health risk, especially to immune-compromised humans and animals. Public education on the zoonotic implication of this protozoan infection is of paramount importance in Enugu State, in particular, and Nigeria, in general, considering the closeness of dogs and man.

Keywords: Cryptosporidium, dogs, modified Ziehl-Neelsen techniques, Nigeria, prevalence, risk factor.

Antigenic site of nucleoprotein gene from Indonesian rabies virus isolates

Research (Published online: 31-05-2019)

16. Antigenic site of nucleoprotein gene from Indonesian rabies virus isolates

Jola Rahmahani, Suwarno Suwarno, Wiwik Misaco Yuniarti and Fedik Abdul Rantam

Veterinary World, 12(5): 724-728

ABSTRACT

Background and Aim: Several molecular studies on rabies virus (RABV) have been conducted in Indonesia, but it does not give clear information about molecular characteristics of previous RABV isolate in Indonesia. This study was conducted to know the characteristic of circulating RABV to determine a suitable method to control the spreading of RABV in Indonesia.

Materials and Methods: Samples of infected RABV from dog brain were collected from Sumatera, Kalimantan, Sulawesi, and Bali Islands. All samples were examined based on nucleoprotein encoding gene to determine the molecular characteristics based on homology and phylogenetic tree compared to Pasteur Virus and RABV that came from another country within Asia (Indonesia, China, Thailand, India, and Korea). The collected samples were processed by one-step reverse transcriptase-polymerase chain reaction using nucleoprotein encoding gene followed by sequencing. The amino acid of its antigenic site of isolated RABV was also analyzed.

Results: The results showed that isolated RABV has 84-85% similarity compared to Pasteur. According to phylogenetic construction, isolated samples do not share the same lineage toward Pasteur. The homology scores of isolated samples compared to RABV within Asia such as Indonesia, China, Thailand, India, and Korea were 98-99%, 92-93%, 88-89%, 86-88%, and 85-88%, respectively. According to antigenic site analysis compared to Pasteur, it was found that there were amino acid mutations within antigenic Site IV of nucleoprotein. Amino acid mutation from isoleucine to valine occurred in amino acid number 240 of 6 Kalimantan, 7 Kalimantan, and 8 Kalimantan. Amino acid mutation from alanine to aspartate and asparagine to threonine occurred within the same antigenic site in amino acid number 246 and 273 of C4 isolate from Sulawesi.

Conclusion: According to homology and phylogenetic tree analyses, isolated RABV remained different compared to RABV within Asia and Pasteur. The amino acid mutation occurred in antigenic site of nucleoprotein encoding gene.

Keywords: amino acid, antigenic site, N gene, rabies virus.

Surveillance of the equine infectious anemia virus in Eastern and Central Saudi Arabia during 2014-2016

Research (Published online: 29-05-2019)

15. Surveillance of the equine infectious anemia virus in Eastern and Central Saudi Arabia during 2014-2016

Abdulmohsen Abdullah Alnaeem and Maged Gomaa Hemida

Veterinary World, 12(5): 719-723

ABSTRACT

Background: Equine infectious anemia virus (EIAV) is one of the most important threats to the equine industry globally. This is due to the poor performance of the affected horses, which requires euthanization of the infected animals upon the infection confirmation. Infected animals remain carriers throughout their life. EIAV infection has been reported in many parts of the world, including North America, Europe, Asia, and Africa. However, the EIAV status is never assessed in horses in the Gulf area, especially in the Kingdom of Saudi Arabia (KSA).

Aim: This study aimed to perform molecular and serological surveillance among some horse populations in Eastern and Central Saudi Arabia.

Materials and Methods: Sera and whole blood were collected from 361 horses and 19 donkeys from the eastern and central regions of Saudi Arabia during January 2014-December 2016. Sera were tested by the commercial EIAV enzyme-linked immunosorbent assay kits. Moreover, the collected blood samples were tested by the commercial real-time polymerase chain reaction kits.

Results: Our serological surveillance revealed the absence of any antibodies against EIAV in the tested animals. Similar results were reported for the tested horses' plasma. This study confirms the absence of EIAV in horses and donkeys from Eastern and Central Saudi Arabia during the tenure of the current study. However, careful monitoring of the EIAV is highly recommended to avoid the emergence of such a virus in the horse population in Saudi Arabia.

Conclusion: To the best of our knowledge, this is the first EIAV surveillance conducted not only in Saudi Arabia but also in the Gulf area. This study confirms the absence of EIAV in the tested equine population in the eastern and central regions of Saudi Arabia during 2014-2016.

Keywords: enzyme-linked immunosorbent assay, equine infectious anemia virus, real-time polymerase chain reaction, Saudi Arabia.

Serological and histopathological investigation of brucellosis in cattle in Medea region, Northern Algeria

Research (Published online: 28-05-2019)

14. Serological and histopathological investigation of brucellosis in cattle in Medea region, Northern Algeria

El Aid Kaaboub, Nassim Ouchene, Nadjet Amina Ouchene-Khelifi and Djamel Khelef

Veterinary World, 12(5): 713-718

ABSTRACT

Aim: This study was performed to determine the prevalence of bovine brucellosis in Medea region, Northern Algeria.

Materials and Methods: The study was carried out on 495 non-vaccinated cattle, of which 280 (30 males and 250 females) belonged to 57 cattle farms and 215 cows were sampled at abattoirs of Medea. Sera collected from the cattle were tested using the Rose Bengal test and confirmed by histopathological analysis.

Results: Serological examination revealed that 7/57 farms (12.28%) were infected, of which 7/280 (2.5%) cattle were seropositive. The prevalence in females and males was 2.4% (6/250) and 3.33% (1/30), respectively. No significant difference has been observed between females and males. Older animals (≥8 years) were infected more. The prevalence of infection was 9.1%. Seroprevalence of Brucella infection in cows that have already had abortion was higher compared with non-aborted cows (4.34% and 2.20%, respectively). In abattoirs, a total of 25 (11.62%) seropositive cows were detected, and the histopathological analysis was positive in all these cows.

Conclusion: The study indicates that brucellosis indeed exists in cattle in Medea and shows that the meat of slaughtered cattle tested positive for brucellosis may constitute a real risk of transmission to both butchery personnel and consumers, which requires that the meat of infected animals should be analyzed before being marketed.

Keywords: Algeria, Brucella, cattle, histopathological analysis, seroprevalence.