Comparative effects of amiodarone and dronedarone treatments on cardiac function in a rabbit model

Research (Published online: 28-02-2019)

24. Comparative effects of amiodarone and dronedarone treatments on cardiac function in a rabbit model

Worakan Boonhoh, Anusak Kijtawornrat and Suwanakiet Sawangkoon

Veterinary World, 12(2): 345-351

ABSTRACT

Aim: The objective of the study was to compare the effects of amiodarone (AM) and dronedarone (DR) on heart rate variability (HRV) and cardiac contractility in a rabbit model.

Materials and Methods: A total of 16 male New Zealand white rabbits were divided into two groups, treated either with AM or DR at incremental dosages of 50 mg/kg/day (AM50 and DR50) and 100 mg/kg/day (AM100 and DR100), orally administrated for 7 days. At the end of each period, electrocardiograms were recorded during consciousness and analyzed using the short-term time and frequency domains of HRV. Standard echocardiography and speckle-tracking echocardiography were studied during immobilization with xylazine and ketamine.

Results: The results showed that AM100 and DR100 significantly decreased heart rate, total power, low-frequency component, and low-to-high frequency ratio compared with baselines. Most echocardiogram parameters revealed no significant difference from baselines, except for the global circumferential plane strain rate and time to peak standard deviation of strain, which had statistical significances after treating with AM.

Conclusion: Both AM and DR possess negative chronotropy and reduce HRV, which may be explained by their sympathetic suppression and calcium channel blocking activities. Theoretically, both antiarrhythmic drugs may also possess negative inotropy, but only AM is shown to have a negative inotropic effect and reduces cardiac dyssynchrony in this model.

Keywords: Amiodarone, cardiac function, dronedarone, heart rate variability, rabbit.

Resveratrol mitigates hypercholesterolemia exacerbated hyperthermia in chronically heat-stressed rats

Research (Published online: 27-02-2019)

23. Resveratrol mitigates hypercholesterolemia exacerbated hyperthermia in chronically heat-stressed rats

Hosam Al-Tamimi, Amani Al-Dawood, Saddam Awaishesh and Tony Abdalla

Veterinary World, 12(2): 337-344

ABSTRACT

Background and Aim: Hypercholesterolemia (HC) is the major leading cause of cardiovascular disease worldwide. Such atherogenic aberration deeply impacts blood circulation. Resveratrol (R) is a polyphenol that has received attention as a hypolipidemic, antioxidant, and vascular agility advocate. Efficient blood redistribution is a key element in mammalian thermoregulation. We hypothesized that R treatment may aid in mitigating hyperthermic responses under both acute and chronic heat stress (HS) conditions in HC male rats.

Materials and Methods: All rats were initially fitted with miniaturized thermologgers to measure core body temperature (Tcore). With a 2 × 2 factorial arrangement, four groups were randomly allotted, in which half of the animals ingested an HC diet (C+), while the other half ingested a control (C-) diet, throughout the whole study duration of 35 days. Seven rats from each dietary treatment, however, received R (R+; 13 mg/kg BW/day), while the rest received normal saline (R-) for 5 continuous days. All animals were maintained at thermoneutrality (TN; ambient temperature; Ta=23.15±0.04°C) for a period of 30 continuous days (days 0-29). On day 29, an acute HS (HS; Ta=35.86±0.37°C; for 9 nocturnal h) was imposed. Then, from day 29, a chronic HS protocol (Ta=32.28±1.00°C) was maintained until the past day of the trial (day 34), after which blood samples were drawn for analyses of platelet (PL) count, total antioxidant activity (TAO), total cholesterol (TC), triglycerides (TGs), and lipid peroxidation (LP).

Results: Switching animals from TN to HS resulted in abrupt rises in Tcore. The HC diet induced a significant (p<0.01) hyperlipidemia over the control of diet-consuming rats. Interestingly, the hyperthermic response to acute HS was highly pronounced in the rats consuming the C- diet, while the C+ diet exacerbated the chronic HS-induced hyperthermia. Despite failure to improve TAO in the C+ diet, R+ treatment caused a marked (p<0.05) decline in nighttime - hyperthermia in C+ rats, likely by enhancing blood flow to extremities (for heat dissipation) as delineated by drastic downregulations of C+ related rises in PL, TC, TG, and LP (HC diet by R+ interaction; p<0.03).

Conclusion: The hyperthermic response in C- groups was attributed to higher amount of feed intake than those consuming the C+ diet. Yet, the R+ improvement of thermoregulation in the C+ group was likely related to enhancement of vascular hemodynamics. Resveratrol intake mitigated chronic HS-evoked hyperthermia in rats. Such an approach is worthy to follow-up in other mammals and humans.

Keywords: heat stress, hypercholesterolemia, rats, resveratrol, thermoregulation.

Isolation and molecular characterization of Mannheimia haemolytica and Pasteurella multocidaassociated with pneumonia of goats in Chhattisgarh

Research (Published online: 24-02-2019)

22. Isolation and molecular characterization of Mannheimia haemolytica and Pasteurella multocidaassociated with pneumonia of goats in Chhattisgarh

Nidhi Rawat, Varsha Rani Gilhare, Krishna Kumar Kushwaha, Deeksha Dipak Hattimare, Foziya Farzeen Khan, Rajesh Kumar Shende and Dhananjay Kumar Jolhe

Veterinary World, 12(2): 331-336

ABSTRACT

Aim: The purpose of this study was to isolate and characterize the Mannheimia haemolytica and Pasteurella multocida from blood, nasal discharge, and lung tissue of pneumonic goats.

Materials and Methods: A total of 14 goats were investigated for pneumonic pasteurellosis. Of 14 goats, nasal swabs and blood samples were collected from 10 clinically diseased animals. Moreover, lung tissue and heart blood samples were collected during necropsy of four goats died with pneumonia. All the samples were processed for the isolation of M. haemolytica and P. multocida in the laboratory. Bacterial isolates were identified by cultural and biochemical characters and 16S rRNA sequence analysis. All the isolates were subjected to susceptibility testing using commonly used antimicrobials. M. haemolytica isolates were characterized by PHSSA gene detection. P. multocida isolates were characterized by KMT1 gene detection and capsule typing.

Results: On necropsy of dead goats, the pneumonia was characterized as acute fibrinous bronchopneumonia. Bacterial culture revealed the isolation of M. haemolytica (7) and P. multocida (5) of 10 clinical cases. Moreover, M. haemolytica and P. multocida were coisolated from two of the lung tissues. Furthermore, one of the other two lung tissues showed the isolation of M. haemolytica while the other showed recovery of P. multocida. Bacterial isolates were specifically identified by the 16S rRNA sequence analysis. The isolates showed reduced susceptibility to β-lactams, aminoglycosides, and fluoroquinolones . Moreover, the PHSSA and KMT1 genes were specifically detected among M. haemolytica, and P. multocida isolates, respectively. All P. multocida isolates belonged to serogroup A.

Conclusion: The present study reported an occurrence of pneumonic pasteurellosis caused by M. haemolytica and P. multocida in a goat flock.

Keywords: bronchopneumonia, goat, Mannheimia haemolytica, Pasteurella multocida, pneumonic pasteurellosis.

Comparative efficacy of two-dimensional mode and color Doppler sonography in predicting gender of the equine fetus

Research (Published online: 23-02-2019)

21. Comparative efficacy of two-dimensional mode and color Doppler sonography in predicting gender of the equine fetus

M. Mebarki, R. Kaidi, A. Azizi and M. Basbaci

Veterinary World, 12(2): 325-330

ABSTRACT

Background and Aim: Ultrasonographic fetal sexing is of utmost economic importance for horse breeders. Relatively, a few studies have been conducted to determine fetal sex in mare using transrectal Doppler ultrasound. This study aimed to compare two sexing techniques, two-dimensional (2D) mode and color Doppler ultrasonography.

Materials and Methods: The study was conducted on 39 mares under field conditions. Examinations were performed using the ultrasonic model device (Medison SonoAce Pico, South Korea), equipped with real-time 3-7 MHz convex multifrequency transducer. Fetal sex diagnosis was carried out in two periods of pregnancy, early period (57-80 days of gestation) and late period (80-150 days of gestation).

Results: No difference (p=0.4) was observed between the efficiency of the 2D mode and Doppler ultrasound in sex determination with the respective frequency of 74% and 85%. The best time to sex the early fetus was between 57 and 70 days of gestation and between 90 and 120 days for the older fetus.

Conclusion: The accuracy of sex determination can be increased using Doppler mode compared to 2D mode, especially in the early period between 57 and 70 days, in male more than female sex.

Keywords: Doppler mode, fetal sex, mare, two-dimensional mode, ultrasound.

Chemotherapeutic control of Gram-positive infection in white sea bream (Diplodus sargus, Linnaeus 1758) broodstock

Research (Published online: 23-02-2019)

20. Chemotherapeutic control of Gram-positive infection in white sea bream (Diplodus sargus, Linnaeus 1758) broodstock

Nadia G. M. Ali, Ibrahim M. Aboyadak and Heba S. El-Sayed

Veterinary World, 12(2): 316-324

ABSTRACT

Aim: This study aimed to identify the pathogenic bacteria responsible for the septicemic disease affecting white sea bream brooders and determining the sensitivity of the recovered isolates to different antibiotics followed by estimation of long-acting oxytetracycline (OTC) efficacy in controlling this disease, and finally, determining the proper dose regimen.

Materials and Methods: Biolog microbial identification system was used for determination of the pathogens which are responsible for this disease. Agar disk diffusion test and minimum inhibitory concentration (MIC) were used to determine the antibiotic susceptibility of recovered isolates. Oxytetracycline (OTC) was used at a dose level of 100 mg/kg body weight for the treatment of diseased fish, and the OTC concentration in the serum samples was determined by high-performance liquid chromatography.

Results: Fifteen Staphylococcus epidermidis and 11 Bacillus cereus isolates were recovered from the lesion of muscle, tail, eye, and heart blood. S. epidermidis isolates were sensitive to OTC, ciprofloxacin, enrofloxacin, spiramycin, erythromycin (E), and florfenicol. B. cereus isolates were sensitive to all mentioned antibiotics except E. Based on the MIC test, all B. cereus isolates were sensitive to OTC with MIC ranging between <0.125 and 4 μg/ml and 11 S. epidermidis isolates were sensitive with MIC ranging between <0.125 and 8 μg/ml, while four isolates were resistant. Different degrees of degenerative changes were present in the hepatopancreas, posterior kidney, eye, and skin tissues of diseased fish.

Conclusion: Single intraperitoneal injection of long-acting OTC at a dose of 100 mg/kg body weight was effective in termination of S. epidermidis and B. cereus infection in white sea bream (D. sargus) broodstock.

Keywords: Bacillus cereus, histopathology, high-performance liquid chromatography, oxytetracycline, sensitivity, Staphylococcus epidermidis.

Antiretroviral activity of Pterois volitans (red lionfish) venom in the early development of human immunodeficiency virus/acquired immunodeficiency syndrome antiretroviral alternative source

Research (Published online: 23-02-2019)

19. Antiretroviral activity of Pterois volitans (red lionfish) venom in the early development of human immunodeficiency virus/acquired immunodeficiency syndrome antiretroviral alternative source

Andy Noorsaman Sommeng, R. Muhammad Yusuf Arya, Mikael Januardi Ginting, Diah Kartika Pratami, Heri Hermansyah, Muhamad Sahlan and Anondho Wijanarko

Veterinary World, 12(2): 309-315

ABSTRACT

Aim: This study aimed to investigate the antiviral activity of Pterois volitans phospholipase A2 (PV-PLA2) from Indonesia to human immunodeficiency virus (HIV).

Materials and Methods: Fresh venomous fin parts of wild PV specimens were collected from Java Sea waters. Then, it washed using phosphate buffer pH 7.0 and immersed in phosphate buffer pH 7.0 0.01 m containing CaCl2 0.001 m for 24 h. The immersed fin then allowed for extraction process by sonicating for 2×8 min with 80% pulse and 20 kHz output with temperature controlling to avoid denaturation. The crude venom (CV) extracted from the fin is allowed for purification by 80% ethanol (ET) precipitation and ammonium sulfate fractionation method. The purified PV-PLA2 then analyzed using Lowry's method, Marinette's method, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and 3-(4, 5-dimethyl thiazol-2yl)-2, 5-diphenyl tetrazolium bromide assay. After determining the purest and safest sample of six samples analyzed, the chosen sample then tested into simian retrovirus-2 (SRV2)-A549 culture (48×104 cells/mL at 1-4 ppm), and compared to the CV sample (1-4 ppm) and lamivudine (100 ppm). The culture then is analyzed using a quantitative real time-polymerase chain reaction to find out the copy number of SRV-2 virus in each culture.

Results: The protein's activity, concentration, and purity analysis revealed that the PV-PLA2 purified using ammonium sulfate fractionation has the highest activity (1.81 times higher than the CV at 80% fractionation) and has higher purity than the sample from ET fractionation. The testing of the sample purified using ammonium sulfate fractionation at 80% saturation level shown that it has a 97.78% inhibition level toward SRV2-A549 culture at 4 ppm. However, in comparison to lamivudine which has 99.55% inhibition level at 100 ppm, it needs much lower concentration to achieve the same result.

Conclusion: The significant inhibition of SRV2-A549 culture shown that the PV-PLA2 extracted from PV venom has the potential to become anti-HIV substances. It would be worthwhile to further evaluate the antiretroviral activity of PV-PLA2 in the in vivo studies.

Keywords: antiretrovirus, lamivudine, phospholipase A2, Pterois volitans, simian retrovirus serotype 2.

Reproductive performance of pigs raised by intensive management system in Abuja, Nigeria

Research (Published online: 22-02-2019)

18. Reproductive performance of pigs raised by intensive management system in Abuja, Nigeria

Kenneth Owoicho Abah, Joy Iyojo Itodo, Simon Azubuike Ubah and Ibrahim Shettima

Veterinary World, 12(2): 305-308

ABSTRACT

Background: Population growth led to an increase in the number of people raising pigs, resulting in increased demand for piglets/pigs for breeding and pork for consumption.

Aim: This study was carried out to determine the reproductive performance of pigs raised by the intensive management system in Abuja, Nigeria, with a view to assist farmers in ensuring improved productivity and profitability.

Materials and Methods: Using an interview-based questionnaire, data from 121 sows and 649 preweaning piglets were collected in 12 herds, from September 2017 to March 2018. Measures of reproductive and production performance assessed in this study were interfarrowing interval (IFI), number of liveborn piglets (NLB), preweaning piglet mortality (PPM), age at weaning (AAW), weaning to service interval (WSI), age at first farrowing (AFF), number of piglets weaned per litter (NPWL), and number of piglets weaned per sow per year (NPWPY).

Results: The results obtained in this study were IFI 6.2±0.84 months, NLB 7.2±1.11, PPM 31%, AAW 40.2±3.12 days, NPWL 5.3±0.73, WSI 39.4±4.59 days, AFF 9.1±0.60 months, and NPWPY 8.1±1.21. The identified causes of PPM were maternal overlay 31.34%, splay leg/hypoglycemia 22.39%, cannibalism 20.40%, starvation 14.93%, and unknown cause 10.94%.

Conclusion: The result showed that the reproductive performance of the sow (especially, NPWPY and PPM) needs to be improved on. There is a need to promote extension and herd health services by veterinarians and livestock personnel to potential and existing farmers in the area. This is more so because organized pig production in the studied area is relatively new and more people are establishing pig farms in the studied area.

Keywords: Abuja, intensive management system, reproductive performance, swine production.