Seroprevalence of peste des petits ruminants and bluetongue in goat population of Meghalaya, India

Research (Published online: 17-12-2018)

7. Seroprevalence of peste des petits ruminants and bluetongue in goat population of Meghalaya, India

A. Karam, K. Puro, S. Das, I. Shakuntala, R. Sanjukta, A. A. P. Milton, S. Ghatak and A. Sen

Veterinary World, 11(12): 1689-1691

ABSTRACT

Aim: This study aimed to determine the seroprevalence of peste des petits ruminants (PPR) and bluetongue (BT) in goats' population in the state of Meghalaya of Northeast India.

Materials and Methods: The serosurveillance study was done from the random sampling (n=598) of blood collected from five districts (Ri-Bhoi, East Khasi Hills, West Khasi Hills, Jaintia Hills and West Garo Hills) of Meghalaya. The presence of antibodies against PPR and BT in the samples was detected by indirect enzyme-linked immunosorbent assay (ELISA) method for PPR and competitive ELISA for BT.

Results: The results showed the overall seropositivity of PPR and BT at 7.19% and 60.20%, respectively. West Garo Hills recorded the highest seroprevalence of both PPR (9.81%) and BT (68%) and 3.6% of the samples tested positive for both PPR and BT.

Conclusion: The random survey results indicating the presence of PPR and BT have specific implication in epidemiological perspectives since it highlights the prevalence under natural situations, where the subclinical, inapparent, or non-lethal or recovery of infection was suspected in unvaccinated animals. It also warrants further studies to suggest appropriate control measures to prevent the spread of infection.

Keywords: bluetongue, goat, peste des petits ruminants, seroprevalence, virus.

Phenotypic and genotypic drug resistance profile of Salmonella serovars isolated from poultry farm and processing units located in and around Mumbai city, India

Research (Published online: 16-12-2018)

6. Phenotypic and genotypic drug resistance profile of Salmonella serovars isolated from poultry farm and processing units located in and around Mumbai city, India

R. N. Waghamare, A. M. Paturkar, V. M. Vaidya, R. J. Zende, Z. N. Dubal, A. Dwivedi and R. V. Gaikwad

Veterinary World, 11(12): 1682-1688

ABSTRACT

Background and Aim: The extensive use of antimicrobials in poultry has led to an increase in bacterial multidrug resistance, and the emergence of multidrug-resistant nontyphoidal Salmonella is a global problem. This study was performed to detect antibiotic-resistant Salmonella serovars in poultry farming and processing environment.

Materials and Methods: A total of 956 various samples, comprising 432 farm origin, 324 poultry processing stage wise and environmental, and 154 product processing stages and environmental samples, were collected from poultry farms and processing units located in and around Mumbai city. Of a total of 71 recovered isolates, 42 randomly selected Salmonella isolates were subjected for antibiotic susceptibility testing by disk diffusion method and serotyping. A total of 31 serotypically confirmed isolates were characterized for the presence of tetA, tetB, blaTEM, and CTX-M gene.

Results: Higher resistance was recorded against Doxycycline (100%), followed by Oxytetracycline (97.62%), Neomycin (88.10%), Erythromycin (83.33%), Tetracycline (78.57%), and Ceftizoxime (35.71%). Resistance from 0.00 to 26.19 percent was found to antimicrobials, namely Norfloxacin (26.19%), Ampicillin (21.43%), Azithromycin (21.43%), Ciprofloxacin (19.05%), Colistin (4.76%), Streptomycin (16.67%), Cefotaxime (14.19%), Enrofloxacin (14.29%), Amoxyclav (14.29%), Gentamicin (7.14%), Chloramphenicol (4.76%), Amikacin (4.76%), and Ceftazidime (0.0%). Results demonstrate that the Salmonella Virchow dominated and all serotypes were found to carry Tetracycline resistance gene tetA, 5 isolates were found to be positive for blaTEM, whereas none of the isolates were carrying tetB and CTX-M gene.

Conclusion: This study revealed that there is a significant rise of Tetracycline resistance with the presence of tetA gene in Salmonella spp. which indicates selective pressure for adopting resistance against tetracycline group of antibiotics.

Keywords: multidrug-resistant, poultry, Salmonella spp., tetracycline.

The epidemiology of tick in transmission of Enterobacteriaceae bacteria in buffaloes in Marshes of the south of Iraq

Research (Published online: 16-12-2018)

5. The epidemiology of tick in transmission of Enterobacteriaceae bacteria in buffaloes in Marshes of the south of Iraq

Jenan Mahmood Khalaf, Ibrahim Abbas Mohammed and Abdulkarim Jafar Karim

Veterinary World, 11(12): 1677-1681

ABSTRACT

Aim: This study aimed to investigate the role of ticks in transmission of Enterobacteriaceae bacteria in buffaloes in marshes of the south of Iraq.

Materials and Methods: This survey included 255 healthy and clinically ill buffaloes in marshes of the south of Iraq (Thi- Qar, Basra, and Misan provinces) between the periods from May 2017 to April 2018. Animals were clinically examined. Ticks, isolated from perineum and under tail, sent to the Department of Parasitology, College of Veterinary Medicine, University of Baghdad and University of Thi-Qar for taxonomy. Ticks were dissected, and all internal organs were removed aseptically by forceps to sterile tubes containing brain heart infusion broth and incubated at 37°C for 36 h and subcultured on blood and MacConkey agars at 37°C for 36 h. Biochemical tests including citrate, methyl red, indole, urease, triple sugar iron (H2S), motility tests, and Gram stain were performed.

Results: Two species of ticks were identified. Hyalomma spp. (175; 68.63%) were significantly higher than Rhipicephalus spp. (80; 31.37%). Conversely, pathogenic bacteria in Rhipicephalus spp. (55; 68.75%) was higher than detected from Hyalomma spp. (113; 64.57%), but non-significant. The prevalence of Enterobacteriaceae bacteria in ticks on diseased buffaloes (110; 88.00%) was significantly higher than non-diseased (58; 44.61%). Escherichia coli (123; 73.21%) showed a significantly higher prevalence than Salmonella spp. (25; 14.88%) and Klebsiella spp. (15; 8.92%). There was no significant variation between Salmonella spp. and Klebsiella spp. The latter was significantly higher than Enterobacter spp. (5; 2.97%). The isolation rate of infected tick collected from buffaloes inhabiting marshes was 65 (66.32%), 45 (69.23%), and 58 (63.40%) from Thi-Qar, Basra, and Misan provinces, respectively, with no significant variation. July and August (71.05% and 72.97%) reported the highest among months, while November, December, January, and February recorded nil (0.00%). The summer season was significantly higher (72.72%) followed by autumn (62.06%) and spring (59.77%), while winter reported no any bacterial isolation (0.00%).

Conclusion: The high prevalence of Enterobacteriaceae bacteria isolated from hard ticks supports the probability of transmitting these bacteria to buffaloes in marshes of the south of Iraq.

Keywords: epidemiology, ticks, Enterobacteriaceae, buffaloes, Iraq.

Effect of probiotic Pediococcus acidilactici FT28 on growth performance, nutrient digestibility, health status, meat quality, and intestinal morphology in growing pigs

Research (Published online: 14-12-2018)

4. Effect of probiotic Pediococcus acidilactici FT28 on growth performance, nutrient digestibility, health status, meat quality, and intestinal morphology in growing pigs

Mamata Joysowal, B. N. Saikia, Runjun Dowarah, S. Tamuly, D. Kalita and K. B. Dev Choudhury

Veterinary World, 11(12): 1669-1676

ABSTRACT

Aim: The experiment was conducted to evaluate the effect of swine-origin probiotic Pediococcus acidilactici FT28 on growth, nutrient utilization, health status, meat quality and intestinal morphology in growing female pigs.

Materials and Methods: Pigs (n=27) were distributed into three groups (3 replicates of 3 each) and supplemented with basal diet either without probiotics (C) or with a probiotic of dairy-based (Lactobacillus acidophilusNCDC-15; TLact) or swine based (P. acidilactici FT28; TPedic). The probiotics were fed as fermented feed at 200 g/pig/day. At the end of the trial, six pigs from each group were selected for metabolism trial and then sacrificed to determine meat quality and intestinal morphology.

Results: Supplementation of both probiotics improved growth performance, whereas feed intake, digestibility of CP and N retention were better (p<0.05) in P. acidilactici FT28-fed group. However , the digestibility of dry matter (DM), organic matter (OM), ether extracts (EE), crude fibre and nitrogen free extract did not show any significant effect on probiotic supplementation. The serum A: G ratio, triglyceride, and cholesterol level were also improved (p<0.05) in TPedic group compared to other treatment groups. Both probiotic supplementations showed lower (p<0.05) serum glucose level with similar protein and albumin value, which indicated good utilization of feed as well as health status of growing pigs. Dressing percentage, vital organ weight, and EE of loin meat were higher (p<0.05) in probiotic-supplemented groups compared to control. However, P. acidilactici FT28-fed animals showed higher (p<0.05) CP and total ash percentage of meat without affecting pH, water holding capacity, and extract release volume of loin muscle. The villi height and crypt depth were better in both supplemented groups compared to control.

Conclusion: Results of the present study revealed that P. acidilactici FT28 could serve as better probiotic source in swine production for the better utilization of CP and N-retention in meat with improved health status and intestinal morphology.

Keywords: growth performance, intestinal morphology, meat quality, Pediococcus acidilactici, pig.

Mycoplasma bovis arthritis and pneumonia in calves in Jordan: An emerging disease

Research (Published online: 12-12-2018)

3. Mycoplasma bovis arthritis and pneumonia in calves in Jordan: An emerging disease

Wael M. Hananeh, Waleed M. Al Momani, Mustafa M. Ababneh and Sameeh M. Abutarbush

Veterinary World, 11(12): 1663-1668

ABSTRACT

Aim: Clinical, microbiological, molecular, and pathological assays were undertaken to characterize an outbreak of increasingly reported signs of unresponsive arthritis and pneumonia of Mycoplasma bovis infection in young calves in Jordan.

Materials and Methods: Clinical history of the affected bovine herd was investigated for the presence of respiratory and/ or joint problems. Two calves with such history were clinically examined and necropsied. Representative tissues were sent for microbiological, molecular, and pathological examinations for M. bovis infection.

Results: The outbreak started in a herd of 220 nursing calves, 2 months before the receiving of two calves for postmortem examination. Clinically, respiratory signs and infection of one or more joints dominated in the affected calves. The morbidity and case fatality rates were 27.27% and 61.7%, respectively. The left carpal joint was markedly swollen in both calves and exhibited necrofibrinous to granulomatous arthritis in varying degrees of severity. The anteroventral lung lobes in both calves were consistently affected and revealed multifocal to coalescing severe necrogranulomatous and fibrinopurulent bronchopneumonia. Microbiological and molecular findings confirmed the pathological examination. Furthermore, bovine viral diarrhea (BVD) was diagnosed in one calf by histopathology and polymerase chain reaction.

Conclusion: This investigation reports the first outbreak of M. bovis infection in calves located in Jordan that could occur concurrently with BVD.

Keywords: arthritis, bovine, Mycoplasma bovis, pathology, pneumonia.

An investigation of the effect of Zataria multiflora Boiss and Mentha piperita essential oils to improve the chemical stability of minced meat

Research (Published online: 11-12-2018)

2. An investigation of the effect of Zataria multiflora Boiss and Mentha piperita essential oils to improve the chemical stability of minced meat

Mojtaba Raeisi, Mohammad Hashemi, Majid Aminzare, Asma Afshari, Tayebeh Zeinali and Behrooz Jannat

Veterinary World, 11(12): 1656-1662

ABSTRACT

Background and Aim: Extending the shelf life of foods is an essential concept in food safety. Most of the time, foods deteriorate through the growth of microorganisms or oxidation process. Essential oils (EOs) derived from plant material have well-documented antioxidant and antibacterial activity. This study aimed to evaluate the effect of Zataria multiflora Boiss EO (ZEO) and Mentha piperita EO (MEO) on the chemical stability of minced meat during storage at 7°C.

Materials and Methods: Total phenolic content, β-Carotene bleaching test, ferric reducing antioxidant potential assay, and 2,2-Diphenyl-1-picrylhydrazyl radical scavenging activity were used to determine the antioxidant potential of EOs. Five different groups including control, ZEO 0.3%, ZEO 0.5%, MEO 0.3%, and MEO 0.5% were designed to assess the chemical stability of minced meat by measuring pH, thiobarbituric acid (TBA), total volatile base nitrogen (TVBN), and peroxide value (PV).

Results: pH did not have any significant change during storage. TBA values in the control group were significantly higher than the treatment groups, especially from the 5th day of storage. TVBN in the treatment group was significantly lower than the control group during storage. PV values in the treatment group were significantly lower than the control group during storage.

Conclusion: Results indicate that ZEO and MEO had an excellent antioxidant activity and retarded the spoilage process in minced meat.

Keywords: antioxidant, Mentha piperita, minced meat, Zataria multiflora Boiss.

Epidemiology and economic loss of fasciolosis and dicrocoeliosis in Arak, Iran

Research (Published online: 10-12-2018)

1. Epidemiology and economic loss of fasciolosis and dicrocoeliosis in Arak, Iran

Mohsen Arbabi, Elnaz Nezami, Hossein Hooshyar and Mahdi Delavari

Veterinary World, 11(12): 1648-1655

ABSTRACT

Aim: Fasciolosis and dicrocoeliosis are important parasitic diseases worldwide, causing significant financial losses due to decrease in production and viscera condemnation in animals. We performed the current research to assess the epidemiology of these infections and determine their significance from an economic perspective in Arak, Iran.

Materials and Methods: In total, we evaluated 118,463 sheep, 207,652 goats, and 43,675 cattle through necropsic analysis at the slaughterhouses. The average weight of sheep, goat, and cattle liver was 1000, 900, and 5000 g, respectively. The average price of liver in the market was 8 USD/kg. Moreover, the elimination of fundamental nutrients and vitamins was evaluated in infected livers. The prevalence of fasciolosis and dicrocoeliosis was determined. Analysis of variance test was applied for the statistical analysis, and the significance level was <0.05.

Results: In total, Fasciola hepatica and Dicrocoelium dendriticum were detected in 0.56% (confidence interval CI, 0.54-0.59) and 0.77% (CI, 0.75-0.81) of the animals, respectively (p=0.1). The annual economic loss attributed to fasciolosis and dicrocoeliosis was 26698.4 and 30479.2 USD, respectively. The total economic loss was 10,880, 9079.2, and 10,520 dollars in sheep, cattle, and goats, respectively. On the other hand, financial loss resulting from fasciolosis was 7160, 6098.4, and 13,440 dollars in sheep, goats, and cattle, respectively. In addition, economic loss due to dicroceliasis was 10,880, 9079.2, and 10,520 dollars, respectively.

Conclusion: Overall, fasciolosis and dicrocoeliosis in Iran always remain common in sheep, goats, and cattle that afford major economic loss of all the country also exist in Arak province. The present study could provide basic information for further examination of liver fluke infections in Iran.

Keywords: dicrocoeliosis, economic loss, epidemiology, fasciolosis, Iran, slaughtered animal.