Molecular detection and immunopathological examination of Deltapapillomavirus 4 in skin and udder of Egyptian cattle

Research (Published online: 10-07-2018)

5. Molecular detection and immunopathological examination of Deltapapillomavirus 4 in skin and udder of Egyptian cattle

Emad Beshir Ata, Mohamed Abd El-Fatah Mahmoud and A. A. Madboli

Veterinary World, 11(7): 915-920

ABSTRACT

Aim: Bovine papillomaviruses (BPVs) are the main cause of bovine papillomatosis resulting in cutaneous and/or mucosal benign tumors that could be transformed to malignant ones with marked economic importance, especially in the dairy farms. Molecular, pathological, and immunohistochemical (IHC) diagnosis of bovine papillomatosis cases was conducted to identify and characterize the circulating BPV genotype in some Egyptian governorates.

Materials and Methods: Wart-like lesions in skin, udder, and teats were collected from 123 infected cases in Giza, Beni Suef, and El Menoufia Governorates, Egypt, during 2016-2017. Pathological and IHC characterization, molecular identification, genotyping, and phylogenetic analysis based on the conserved late (L1) gene of the all samples were carried out.

Results: 89 of the 123 collected samples (72.3%) were positively detected by polymerase chain reaction (PCR). The sequence analysis of the obtained PCR amplicons was identical revealing identification and genotyping of only one type (Deltapapillomavirus 4 isolate EGY 2017) with accession number (MG547343) which found to be closely related to the recently detected Deltapapillomavirus 4 isolate 04_asi_UK (accession no. MF384288.1) and isolate Deltapapillomavirus 4 isolate 25_equ_CH (accession no. MF384286.1) with 99% nucleotide sequence identity. Histopathological examination revealed severe hyperkeratosis in stratum corneum and acanthosis in most of the cases. These tissue changes were confirmed by the presence of golden brown stained proliferating cell nuclear antigen which was localized intranuclear and perinuclear in other cells using IHC Technique.

Conclusion: It is the first time to detect and genotype the BPVs in these areas with no record of previous genotyping in the whole country. The obtained results will highlight the importance of this disease.

Keywords: bovine papillomaviruses, characterization, Egypt, genotyping, immunohistochemical, pathological, phylogeny.

Effects of various surgical procedures on biochemical parameters of Nigerian dogs and their clinical implications

Research (Published online: 08-07-2018)

4. Effects of various surgical procedures on biochemical parameters of Nigerian dogs and their clinical implications

Aboh Iku Kisani, Terzungwe Tughgba and Abdullahi Teleh Elsa

Veterinary World, 11(7): 909-914

ABSTRACT

Aim: The importance of physical and psychological stress caused by pain during surgery cannot be overemphasized. The aim of this study was to assess the effects of ovariohysterectomy (OVH), gastrotomy (GST), and intestinal resection and anastomosis (IRA) on biochemical parameters of Nigerian dogs anesthetized with the xylazine-propofol combination.

Materials and Methods: A total of 12 dogs were randomly divided into three groups of four each. The animals were treated with xylazine and propofol anesthetics for OVH, GST, and IRA in Groups 1-3, respectively. Blood samples were collected at 0 h, 2, 24, 48, 72, 96, 120, and 144 h postsurgery for determination of cortisol (CORT), glucose (GLU), total protein (TP), albumin (ALB), globulin (GL), and ALB/GL ratio.

Results: There were significant increases (p<0.05) in mean CORT concentrations 2 h postsurgery in the three groups and at 24 h in Group 3 and 96 h in Groups 1 and 3, respectively. GLU concentrations increased significantly (p<0.05) in the three groups at 2 h postsurgery. The mean protein concentrations in Groups 2 and 3 decreased significantly (p<0.05) at 2h and 24 h and 72 h, 96 h, 120 h, and 144 h in Group 3, respectively. There was significant decrease (p<0.05) in the mean ALB concentrations at 2 h, 24 h, 72 h, 96 h, 120 h, and 144 h postsurgery. There was a significant difference (p<0.05) in the mean GL concentrations in Group 3 at 24 h, 72 h, 96 h, and 144 h. All other parameters were not significantly different (p>0.05) in comparison with the control.

Conclusion: Xylazine-propofol combination could decrease humoral immune status and increase serum GLU level invariably portending a high risk of diabetes in the vulnerable dogs.

Keywords: anesthesia, cortisol, stress response, surgery.

Antimicrobial residue occurrence and its public health risk of beef meat in Debre Tabor and Bahir Dar, Northwest Ethiopia

Research (Published online: 07-07-2018)

3. Antimicrobial residue occurrence and its public health risk of beef meat in Debre Tabor and Bahir Dar, Northwest Ethiopia

Birhan Agmas and Mulugojjam Adugna

Veterinary World, 11(7): 902-908

ABSTRACT

Background and Aim: Antimicrobial residues are the parent compounds, their metabolites, and associated impurities of veterinary drugs in any edible portion of an animal product. It can result in severe consequences in human if it is consumed concentration level higher than the standard residue limits. This study aimed to determine the prevalence and its public health risk of antimicrobial residue in fresh beef meat at Bahir Dar and Debre Tabor towns, Northwest Ethiopia.

Materials and Methods: A cross-sectional study was conducted from June to November 2017. The data were collected through interview questionnaire survey and laboratory experiment using Premi® (R-Biopharm, Germany) test Kit. Data were entered; analyzed using SPSS version 20.0.

Results: The result of this study showed that almost all beef farms 42 (97.67%) were using tetracycline (oxytetracycline). In addition to β-lactams, (pinstripe) 21 (48.84%), and sulfonamides drugs including sulfadimidine and diminazene aceturate each 4 (9.30%). No one beef farm has respected drug withdrawal period and lack of awareness about antimicrobial side effects in 37 (86.05%) farms. Of 250 beef cattle slaughtered, antimicrobial residue positivity were 191 (76.4%) giving a 95% confidence interval of 71.10-81.70%. Origin of beef farm system was not significantly associated (p>0.05) with antimicrobial residue positivity.

Conclusion: Prevalence of antimicrobial residue in beef meat in Bahir Dar and Debre Tabor towns were high and also the drug residue detected was higher than the standard level. It implies that; it has the public health hazard.

Keywords: antimicrobial residue, beef, Ethiopia, with drawl time.

Production and characterization of Newcastle disease antibody as a reagent to develop a rapid immunodiagnostic test tool

Research (Published online: 05-07-2018)

2. Production and characterization of Newcastle disease antibody as a reagent to develop a rapid immunodiagnostic test tool

Dwi Desmiyeni Putri, Ekowati Handharyani, Retno Damajanti Soejoedono, Agus Setiyono and Okti Nadia Poetri

Veterinary World, 11(7): 895-901

ABSTRACT

Aim: This research was conducted to produce and characterize ND antibody as reagent candidate to develop a rapid immunodiagnostic test tool.

Materials and Methods: Four New Zealand White rabbits were used in this study and divided into two groups. First group was injected by Sato ND antigen, and second group was injected by genotype VII ND antigen. This study is divided into three steps: (a) ND antibody production, (b) ND antibody purification, and (c) ND antibody characterization. First group was rabbit injected by Sato NDV (5x108.25 egg lethal doses (ELD)50/ml) and second group was injected by genotype VII NDV (5x106.5 ELD50/ml). Antigen induction was performed by subcutaneous administrated for first (day 1) and second (day 14) injection and intravenous administrated for third (day 30) injection. Blood was collected on day 8 after third injection.

Results: Antibody production increased on second antigen injection and reached a peak on day 9 after second antigen injection. Sato and genotype VII ND antibody can be produced without adjuvant within 38 days with the highest titer 210. Based on antibody titer data, both antigens induced antibody production in a similar trend. The characterization antibody by SDS-PAGE indicated that molecular weight of immunoglobulin G (IgG) is 154.93 kDa (whole IgG), heavy chain 54.39 kDa, and light chain 27.74 kDa. ND antibodies have specificity to homologous and heterologous NDVs in varying virulence.

Conclusion: Sato and genotype VII ND antibodies have been successfully produced within 38 days without adjuvant. Specificity of ND antibodies to NDVs in varying virulence and cross-reaction between Sato ND antibody and genotype VII ND antibody indicates that the characterized ND antibodies can be used as a reagent to develop rapid immunodiagnostic test tools.

Keywords: antibody, cross reaction, reagent, SDS-PAGE.

Characterization of the onset of puberty in Tazegzawt lambs, an endangered Algerian sheep: Body weight, thoracic perimeter, testicular growth, and seminal parameters

Research (Published online: 02-07-2018)

1. Characterization of the onset of puberty in Tazegzawt lambs, an endangered Algerian sheep: Body weight, thoracic perimeter, testicular growth, and seminal parameters

Farid Moulla, Rachid El-Bouyahiaoui, Rebouh Nazih, Norezzine Abdelaziz, Nacera Zerrouki and Mokrane Iguer-Ouada

Veterinary World, 11(7): 889-894

ABSTRACT

Aim: The aim of the present study was to define the onset of puberty in Tazegzawt ram lambs, an Algerian sheep breed in endangered status with a small population in its local area.

Materials and Methods: Body growth (body weight and thoracic perimeter), scrotal circumference (SC), penis development stages, and seminal parameters (volume, concentration, and motility) were measured. Data were recorded at fortnightly intervals in 10 animals from 9 to 49 weeks of age.

Results: On the basis of seminal analyses, puberty occurred between 29 and 45 weeks of age. At 29 weeks of age, 30% of lambs reached puberty, and at 45 weeks of age, puberty was observed in 100% of the analyzed animals. Body weight appeared as the most determinant factor, and the onset of puberty was observed when animals reached 43.2±6.4 kg body weight with 25.8±3.7 cm of SC. Seminal analyses revealed that all parameters increased regularly from puberty onset except for sperm concentration. The mean semen volume during the study period was 0.48±0.33 mL with 0.84±0.6 mL at 37 weeks of age. Sperm concentration evolved similarly as semen volume; at 29 and 43 weeks of age, the sperm concentration was 942x106 and 1904x106 spermatozoa/mL, respectively. Kinematic parameters including the percentage of motility, the percentage of progressive motility, and gametes velocities as determined by Computer-Aided Sperm Analyzer showed the highest values at 49 weeks of age.

Conclusion: The current results revealed that, in Tazegzawt ram lambs, puberty occurs between 29 and 45 weeks when animals reach 43.2±4.6 kg body weight.

Keywords: body weight, puberty onset, scrotal circumference, seminal parameters, Tazegzawt breed sheep.

Reproduction performance and blood biochemical parameters in dairy cows: Relationship with oxidative stress status

Research (Published online: 30-06-2018)

23. Reproduction performance and blood biochemical parameters in dairy cows: Relationship with oxidative stress status

Sofiane Boudjellaba, Lynda Ainouz, Safia Tennah, Soraya Temim and Mokrane Iguer-Ouada

Veterinary World, 11(6): 883-888

ABSTRACT

Background and Aim: During the last decades, reproduction performances declined dramatically worldwide, but little is known concerning the involvement of oxidative stress as a causative factor. Oxidative stress may act at different levels, with negative impacts on cell membrane integrity and other active molecules with potential subsequent effects on reproduction. The aim of the current study was to investigate the oxidative stress status in cows according to their reproductive performances.

Materials and Methods: Peripheral blood concentration of two oxidative stress biomarkers, glutathione S-transferase (GST) and malondialdehyde (MDA), and other biochemical parameters (glucose, total lipids, cholesterol, triglycerides, albumin, total proteins, calcium, urea, creatinine, direct bilirubin, alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase) were determined in 40 healthy cows. Body condition score (BCS), calving to first service interval (FSI), calving to conception interval (CCI), and the number of service per conception (SPC) were simultaneously recorded for each cow.

Results: Concerning FSI, three groups were established: Group 1 (from 44 to 60 days), Group 2 (from 60 to 70 days), and Group 3 (from 70 to 80 days). For CCI, two groups were considered: Group 1 (<110 days) and Group 2 (>110 days). MDA showed significant high values only in cows with the lowest BCS (1.5) compared to cows with BCS note of 2.5 and 3.5. No significant difference was observed in cows oxidative stress status (MDA and GST) according to reproductive performances (FSI, CCI, and SPC) in all studied groups.

Conclusion: The results revealed relatively altered oxidative stress status in cows with abnormal reproductive performances; however, no significant difference was recorded whatever the considered reproductive parameter.

Keywords: cow, glutathione S-transferase, malondialdehyde, reproduction performance.

Hematological parameters and selected intestinal microbiota populations in the Indonesian indigenous crossbred chickens fed basal diet supplemented with multi-strain probiotic preparation in combination with vitamins and minerals

Research (Published online: 29-06-2018)

22. Hematological parameters and selected intestinal microbiota populations in the Indonesian indigenous crossbred chickens fed basal diet supplemented with multi-strain probiotic preparation in combination with vitamins and minerals

Sugiharto Sugiharto, Turrini Yudiarti, Isroli Isroli, Endang Widiastuti and Hanny I. Wahyuni

Veterinary World, 11(6): 874-882

ABSTRACT

Aim: The aim of this study was to investigate the effect of dietary supplementation with multi-strain probiotic preparation in combination with vitamins and minerals on the hematological parameters and selected intestinal microbiota populations in the Indonesian indigenous crossbred chickens.

Materials and Methods: A total of 240 one-day-old Indonesian indigenous crossbred chicks were raised for 10 weeks. The chicks were distributed to one of four groups, i.e., chicks receiving basal diet without any additive (CONT), chicks receiving basal diet with 0.04% of zinc bacitracin (AGP), chicks receiving basal diet with 0.01% of commercial probiotic Bacillus subtilis preparation (PROB1), and chicks receiving basal diet with 0.5% of multi-strain probiotic preparation in combination with vitamins and minerals (PROB2). Blood was collected on the week 8, while the internal organs and eviscerated carcasses were collected on the week 10.

Results: PROB2 tended (p=0.09) to have a lower body weight (BW) compared to CONT chicks. Feed conversion ratio was higher (p<0.05) in PROB1 and PROB2 compared to CONT birds. The number of thrombocytes tended (p=0.09) to be higher in CONT than in other groups. Antibody titer against Newcastle disease virus vaccine was higher (p<0.05) in PROB1 and PROB2 than in CONT group. Serum triglyceride concentration was lower (p<0.05) in PROB2 than in other birds. AGP chicks had lower (p<0.05) serum total protein and globulin concentrations than CONT and PROB1 chicks. Serum albumin level was lower (p<0.05) in PROB2 than in CONT and PROB1 birds. Albumin to globulin ratio tended (p=0.06) to be higher in AGP than in other birds. Lactose-negative Enterobacteriaceae tended (p=0.07) to be lower in PROB1 and PROB2 than in CONT group. PROB1 and PROB2 tended (p=0.06) to have greater lactic acid bacteria (LAB) population than in CONT and AGP birds.

Conclusion: Multi-strain probiotic preparation in combination with vitamins and minerals was able to improve immune response and control the potentially pathogenic bacteria. However, the additive could not improve the growth performance of the Indonesian indigenous crossbred chickens.

Keywords: growth performance, Indonesian indigenous crossbred chickens, intestinal bacteria, minerals, multi-strain probiotic, vitamins.