Isolation and identification of Mannheimia haemolytica by culture and polymerase chain reaction from sheep's pulmonary samples in Shiraz, Iran

Research (Published online: 17-05-2018)

10. Isolation and identification of Mannheimia haemolytica by culture and polymerase chain reaction from sheep's pulmonary samples in Shiraz, Iran

Mohammad Tabatabaei and Fatemeh Abdollahi

Veterinary World, 11(5): 636-641

ABSTRACT

Background and Aim: Mannheimia haemolytica is a Gram-negative, non-motile, and non-spore-forming rod-shaped coccobacillus bacterium. On blood agar plate, it shows complete hemolysis. This bacterium constitutes a part of normal flora of the upper respiratory system of ruminants. It is considered as the opportunistic pathogen and the main factor of pneumonic pasteurellosis, which has caused a severe economic loss in sheep and cattle industries. Considering the prevalence of the disease in sheep and goat population in the dry and hot regions of the country in general and in Fars province in particular in the form of pneumonia, the purpose of this study was to isolate and identify the bacterium M. haemolytica from the lung tissues of sheep slaughtered in Shiraz abattoir through culturing and polymerase chain reaction (PCR) methods.

Materials and Methods: In this study, a total of 2500 sheep's lungs were evaluated for finding pneumonic effects. Then, 161 infected pneumonic samples of lung tissues were investigated by culture and PCR methods.

Results: After cultivation, purification, and DNA extraction, 38 samples were found positive for M. haemolytica by cultivation and then all the 38 isolates were confirmed by PCR and multiplex PCR (mPCR). Results of this study indicated that culture and PCR are both practical in identification and isolation of this bacterium though culture is more time-consuming. The utilized mPCR has been more successful in the identification of the bacteria since it requires less time and cost.

Conclusion: In this study, PCR as a superior method among other methods of bacteriology for fast examination of infectious diseases and mPCR, which is a valuable tool for identification of M. haemolytica in clinical samples of animals, was used.

Keywords: Mannheimia haemolytica, pneumonia, polymerase chain reaction, sheep, Shiraz.

The crucial roles of inflammatory mediators in inflammation: A review

Review (Published online: 15-05-2018)

9. The crucial roles of inflammatory mediators in inflammation: A review

L. A. Abdulkhaleq, M. A. Assi, Rasedee Abdullah, M. Zamri-Saad, Y. H. Taufiq-Yap, and M. N. M. Hezmee

Veterinary World, 11(5): 627-635

ABSTRACT

The inflammatory response is a crucial aspect of the tissues' responses to deleterious inflammogens. This complex response involves leukocytes cells such as macrophages, neutrophils, and lymphocytes, also known as inflammatory cells. In response to the inflammatory process, these cells release specialized substances which include vasoactive amines and peptides, eicosanoids, proinflammatory cytokines, and acute-phase proteins, which mediate the inflammatory process by preventing further tissue damage and ultimately resulting in healing and restoration of tissue function. This review discusses the role of the inflammatory cells as well as their by-products in the mediation of inflammatory process. A brief insight into the role of natural anti-inflammatory agents is also discussed. The significance of this study is to explore further and understand the potential mechanism of inflammatory processes to take full advantage of vast and advanced anti-inflammatory therapies. This review aimed to reemphasize the importance on the knowledge of inflammatory processes with the addition of newest and current issues pertaining to this phenomenon.

Keywords: chemokines, cytokines, inflammatory mediators, inflammatory response.

Evaluation of wet cupping therapy on the arterial and venous blood parameters in healthy Arabian horses

Research (Published online: 14-05-2018)

8. Evaluation of wet cupping therapy on the arterial and venous blood parameters in healthy Arabian horses

Turke Shawaf, Wael El-Deeb, Jamal Hussen, Mahmoud Hendi and Shahab Al-Bulushi

Veterinary World, 11(5): 620-626

ABSTRACT

Aim: Recently, the complementary therapies such as cupping and acupuncture are being used in veterinary medicine. This research was carried out to determine the effects of wet cupping therapy (Hijama) on the hematological and the biochemical parameters in the healthy Arabian horses for the first time.

Materials and Methods: In this study, seven clinically healthy Arabian horses were randomly selected. Four points on the animal body were selected to perform the cupping therapy. Two points were selected at the back just behind the scapula on the left and right sides; another two points were located in the rump. Cups with 4 oz (125 ml) size with narrow mouths were used. A manual pump (sucking cups) was used to create the negative pressure within the cups during cupping. Arterial and venous blood parameters and serum cortisol concentration were measured before cupping and 3 days and 2, 4, and 8 weeks after cupping.

Results: No significant differences were estimated in most hematological and biochemical parameters after cupping. A significant decrease in the concentration of serum cortisol was observed in 3 and 14 days after cupping.

Conclusion: Cupping induced minor changes on the hematological and biochemical parameters in Arabian horses. This is the first trial on the effects of wet cupping therapy on the different parameters in Arabian horses, which would be useful for further investigations on the role of complementary therapies in horses. Our further studies will include different disease models.

Keywords: biochemical, cortisol, cupping, hematological, horse.

Assessment of the peste des petits ruminants world epizootic situation and estimate its spreading to Russia

Research (Published online: 13-05-2018)

7. Assessment of the peste des petits ruminants world epizootic situation and estimate its spreading to Russia

Fayssal Bouchemla, Valerey Alexandrovich Agoltsov, Olga Mikhailovna Popova and Larisa Pavlovna Padilo

Veterinary World, 11(5): 612-619

ABSTRACT

Aim: This study focuses on the spatial dynamic associated with the spreading of the peste des petits ruminants (PPR) disease for the past decade (from the year 2007 to 2017), assesses the resulting situation in the world, and has an emphasis on Russian advantages been a PPR host.

Materials and Methods: Outbreaks were confirmed and reported officially by the World Organization for Animal Health (enzyme-linked immunosorbent assay and polymerase chain reaction were used). Data contain the account number of infected, dead, and all susceptible animals in focus of infection in the period of 2007-2017. Once conventional statistical population was defined, a model was installed. Geo-information system QuickMAP was used to clear up the map disease, and through the @Risk program, we got our forecasting value of future situations (by Monte Carlo method).

Results: The spatial study of PPR's occurrence and its spread was mapping according to the incidence of cases and outbreaks. Clusters demonstrated risk levels in the world in the period from 2007 to 2017 year. Based on the epizootological analysis, an assessment of PPR risk and the probability movement of infection in Russia from nearby disadvantaged countries had been carried out. A statistically significant impact of the socioeconomic system on the stationarity index was found equal to 0.63. The PPR risk of spreading could not be ignored. Nevertheless, conducting effective large-scale vaccine companies in a complex of antiepizootic activities against PPR could reduce the risk of spread of the disease up to 91.8%.

Conclusion: Despite all mentioned facts above, the PPR probability can only be reduced by coordinating work of border veterinary services, as in disadvantaged as in free from this disease country, that is, what makes an effective and complete eradication of the disease could be quite realistic.

Keywords: forecast, incidence, outbreaks, peste des petit ruminants, risk factors.

Estimation of deltamethrin residues in cow's and goat's environment and trials to reduce its level in milk

Research (Published online: 11-05-2018)

6. Estimation of deltamethrin residues in cow's and goat's environment and trials to reduce its level in milk

Halla E. K. El Bahgy, Hend A. Elbarbary and Samar S. Ibrahim

Veterinary World, 11(5): 606-611

ABSTRACT

Aim: The present study was aimed to estimate deltamethrin residues in cow's and goat's environment over a certain period of time post-application, to identify the role of both feed and water as a source of pesticides, and to conduct some trials to reduce their levels in milk.

Materials and Methods: A total of 80 water and feed samples (40 of each) and 120 milk samples (80 cow's milk and 40 goat's milk) were collected. Fresh milk samples were collected directly from the udder as well as from feed and water before application and 1st, 2nd, 3rd, 7th, 15th, 21st, and 35th days after insecticide application.

Results: Deltamethrin residues were detected after its application in both water and feed at different levels up to the first 3 days and in all cow's and goat's milk samples at 35th day. The highest levels were detected in milk samples at the 2nd day then at the 7th day followed at the 15th day after application as such levels were above the maximum residual limits. By microwaving the polluted cow's milk samples, deltamethrin residues were not detected without influencing the chemical composition of the milk. However, on freezing of milk, the deltamethrin residues reached 12.6±3.24 μg/L in association with a significant decline in the concentration of fat.

Conclusion: Microwaving of milk is an effective method to decline deltamethrin concentration in milk.

Keywords: deltamethrin, freezing, high-performance liquid chromatography, microwaving treatment, milk, pesticides residues.

A retrospective study of bovine tuberculosis at the municipal abattoir of Bauchi State, Northeastern Nigeria

Research (Published online: 10-05-2018)

5. A retrospective study of bovine tuberculosis at the municipal abattoir of Bauchi State, Northeastern Nigeria

Saleh Mohammed Jajere, Naphtali Nayamanda Atsanda, Asinamai Athliamai Bitrus, Tasiu Mallam Hamisu and Mohammed Dauda Goni

Veterinary World, 11(5): 598-605

Background and Aim: Bovine tuberculosis (bTB) still remains a major zoonotic bacterial disease affecting livestock and humans worldwide. The disease remains a poorly managed tropical disease in most developing countries of the world; where in addition to productivity losses and significance in international trade, it posed a major public health threat to both humans and animals. A retrospective study was designed to investigate the occurrence of bTB lesions at Bauchi municipal abattoir.

Materials and Methods: The study utilized abattoir records spanning a period of 10 years (2004-2013). The records indicated that a total of 1,08,638 heads of cattle comprising n = 56,070 males and n = 52,570 females were slaughtered at the municipal abattoir during the study period.

Results: Of these heads, n = 1230 (1.13%) (95% confidence interval [CI]: 1.07, 1.19) had tuberculous lesions. The annual occurrence during the study period varied significantly (p<0.001) from 0.53% (95% CI: 0.40, 0.67) to 1.87% (95% CI: 1.66, 2.10) in 2010 and 2012, respectively. Females had a significantly higher (p<0.001) prevalence of 2.10% (95% CI: 1.98, 2.23) compared with the males 0.23% (95% CI: 0.19, 0.27). The distribution of suspected gross bTB lesions in different organs showed 11.87% in the lungs, 5.93% in the liver, 1.14% in the heart, and 0.49% accounted for generalized bTB. However, none was observed on the lymph nodes and intestines.

Conclusion: It can be concluded that bTB persists in Bauchi State with annual variations during the study period. This study highlights the importance of meat inspection as an important tool for detecting the presence of bTB lesions.

Keywords: Bauchi, bovine tuberculosis, Mycobacterium bovis, prevalence, retrospective.

Effect of the essential oil of Rosmarinus officinalis (L.) on rooster sperm motility during 4°C short-term storage

Research (Published online: 09-05-2018)

4. Effect of the essential oil of Rosmarinus officinalis (L.) on rooster sperm motility during 4°C short-term storage

L. Touazi, B. Aberkane, Y. Bellik, N. Moula and M. Iguer-Ouada

Veterinary World, 11(5): 590-597

Aim: This study aimed to investigate the protective effect of Rosmarinus officinalis (L.) essential oil on rooster sperm motility during 4°C short-term storage.

Materials and Methods: R. officinalis essential oil was analyzed using gas chromatography coupled to mass spectrometry to identify the active components. 10 of 45-week-old Hubbard commercial broilers were subjected to biweekly semen collections during 3 weeks. At each collection, sperm was pooled and divided into four aliquots and then diluted with Tris extender supplemented with 870, 87, or 8.7 μg/ml of R. officinalisessential oil, identified as treatments R, R5, and R10, respectively. Tris-based extender without any supplementation was considered as a control group. Diluted sperm was then stored at 4°C in the refrigerator and analyzed at 0, 6, 24, and 48 h using a computer-assisted sperm analyzer. Different semen parameters were measured including total motility, progressive motility, gametes velocities (straight line velocity [VSL], curvilinear velocity [VCL], and average path velocity [VAP]), amplitude of the lateral head displacement [ALH], and beat-cross frequency [BCF].

Results: The phytochemical analysis of R. officinalis essential oil revealed the presence of 25 active components including seven major molecules: Camphor (18.88%), camphene (5.17%), 1,8-cineole (7.85%), β-thujene (13.66%), α-thujene (4.87%), chrysanthenone (12.05%), and β-cubenene (7.97%). The results showed a beneficial effect of R. officinalis essential oil on sperm cells motility, particularly when using the lowest concentrations, 8.7 and 87 μg/ml. Progressive motility and gametes velocities (VCL, VSL, and VAP), materializing the quality of gametes motility, showed highly statistically significant values (p<0.01) in 8.7 and 87 μg/ml treatments, especially from 6 h of storage at 4°C. Conversely, the highest concentration (870 μg/ml) showed harmful effects with a total spermicidal activity after 24 h of storage.

Conclusion: The current results revealed the positive impact of R. officinalis essential oil on rooster sperm at 4°C short-term storage probably through fighting against oxidative stress and cold shock damages.

Keywords: antioxidant, avian semen, liquid storage, rosemary essential oil.