Detection of Brucella spp. in milk from seronegative cows by real-time polymerase chain reaction in the region of Batna, Algeria

Research (Published online: 26-03-2018)

17. Detection of Brucella spp. in milk from seronegative cows by real-time polymerase chain reaction in the region of Batna, Algeria

Rabehi Sabrina, Hamdi Taha Mossadak, Mamache Bakir, Meghezzi Asma and Boushaba Khaoula

Veterinary World, 11(3): 363-367

ABSTRACT

Aim: The aim of this study was to detect Brucella spp. DNA in milk samples collected from seronegative cows using the real-time polymerase chain reaction (PCR) assay for diagnosis of brucellosis in seronegative dairy cows to prevent transmission of disease to humans and to reduce economic losses in animal production.

Materials and Methods: In this study, 65 milk samples were investigated for the detection of Brucella spp. The detection of the IS711 gene in all samples was done by real-time PCR assay by comparative cycle threshold method.

Results: The results show that of the 65 DNA samples tested, 2 (3.08%) were positive for Brucella infection. The mean cyclic threshold values of IS711 real-time PCR test were 37.97 and 40.48, indicating a positive reaction.

Conclusion: The results of the present study indicated that the real-time PCR appears to offer several advantages over serological tests. For this reason, the real-time PCR should be validated on representative numbers of Brucella-infected and free samples before being implemented in routine diagnosis in human and animal brucellosis for controlling this disease.

Keywords: Brucella spp., milk, real-time polymerase chain reaction, seronegative cows.

Isolation and identification of bacteria causing mastitis in small ruminants and their susceptibility to antibiotics, honey, essential oils, and plant extracts

Research (Published online: 26-03-2018)

16. Isolation and identification of bacteria causing mastitis in small ruminants and their susceptibility to antibiotics, honey, essential oils, and plant extracts

Abeer Mostafa Abdalhamed, Gamil Sayed Gamil Zeedan and Hala Abdoula Ahmed Abou Zeina

Veterinary World, 11(3): 355-362

ABSTRACT

Aim: The present work aims to isolate and identify bacteria that cause mastitis in small ruminants and evaluates the antibacterial activity of some antibiotics, honey, essential oils, and plant extracts.

Materials and Methods: A total of 289 milk samples were collected from udder secretions of sheep (n=189) and goat (n=100) from El-Fayoum, Beni-Suef, and Giza governorates. Screening subclinical mastitis (SCM) was done using California Mastitis Test (CMT); identification of the isolates was achieved using Gram's staining, hemolytic pattern, colony morphology, and biochemical tests using Analytical Profile Index.

Results: On clinical examination, the incidence of clinical mastitis (CM) was found to be 5.88% and 7% in sheep and goat, respectively. On CMT, SCM was found to be 25 (13.23%) and 11 (10%) in sheep and goat, respectively. Bacteriological examination of all milk samples found the presence of Staphylococcus aureus (SA) (31.1%), coagulase-negative staphylococci (CNS) (19.5%), Escherichia coli (EC) (8.3%), Streptococcus spp. (5.6%), Klebsiella spp. (3.77%), and Pseudomonas spp. (1.89%), while no bacteria were cultured from 81.66% of the samples. Identification of 9 isolates of CNS was achieved by using API staph test to Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus cohnii, and Staphylococcus saprophyticus. The highest bacterial resistance was found in EC (67.14%) followed by Kp (45.28%) and SA (26.57%).

Conclusion: Onion and black cumin essential oils followed by Egyptian honey showed strong antibacterial effects against multidrug-resistant bacteria. Finally, our study proved that Egyptian honey, onion, and black cumin essential oils have a marked strong antibacterial effect against bacteria isolated from small ruminant mastitis, but still further extensive studies are needed to discover the therapeutic properties of these plant extracts and honey.

Keywords: black cumin, essential oil, honey, mastitis, onion, small ruminants, Staphylococcus aureus.

Virulence gene profiling of porcine Pasteurella multocida isolates of Assam

Research (Published online: 21-03-2018)

15. Virulence gene profiling of porcine Pasteurella multocida isolates of Assam

L. Babita Devi, Durlav Prasad Bora, S. K. Das, R. K. Sharma, S. Mukherjee and R. A. Hazarika

Veterinary World, 11(3): 348-354

ABSTRACT

Aim: The present study was conducted to detect and identify the virulence genes in Pasteurella multocida isolates of porcine origin from Assam.

Materials and Methods: A total of 21 porcine P. multocida isolates were subjected to capsular typing and detection of virulence-associated genes (pfhA, tbpA, hgbB, toxA, oma87, ompH, and nanB) using various polymerase chain reaction (PCR) methods reported elsewhere. Further, pathogenicity of the porcine isolates of P. multocida was studied in mice. For each strain of P. multocida selected for pathogenicity trial, the group of mice was injected intraperitoneally (i/p) with 0.1 ml of the inoculum prepared from respective field isolates, containing 109 organisms per ml.

Results: Capsular typing of the isolates by multiplex PCR showed two capsular types, type A (66.66%) and type D (33.33%). All the isolates were positive for outer membrane protein genes, oma87 and ompH genes. Iron acquisition genes, tbpA and hgbB, were detected in 14.28% and 19.04% of the isolates. The dermonecrotoxin encoding gene, toxA, was present in 23.80% of the isolates. Filamentous hemagglutinin encoding gene, pfhA, was detected in 28.57%. The virulence gene distribution pattern of the isolates indicates the important role of the genes in disease pathogenesis.

Conclusion: From the present study, it can be concluded that toxA gene is an important marker gene for defining the pathogenic potential of P. multocida strains in swine.

Keywords: capsular type, Pasteurella multocida, porcine, virulence-associated genes.

Antibacterial activity of fig leaf (Ficus carica Linn.) extract against Enterococcus faecalis and its cytotoxicity effects on fibroblast cells

Research (Published online: 20-03-2018)

14. Antibacterial activity of fig leaf (Ficus carica Linn.) extract against Enterococcus faecalis and its cytotoxicity effects on fibroblast cells

Intan Nirwana, Devi Rianti, R. Helal Soekartono, Rr. Dwi Listyorini and Desi Putri Basuki

Veterinary World, 11(3): 342-347

ABSTRACT

Background: Enterococcus faecalis is one of the bacteria that commonly found in root canal and pulp infection after root canal treatment. Sodium hypochlorite is the most widely used root canal irrigation, but it has toxic properties if exposed to periradicular tissues. It is necessary to develop an alternative for root canal irrigation. Fig leaf (Ficus carica Linn.) extract contains active substances such as flavonoid, tannin, and terpenoid which have been known for their antibacterial potency.

Aim: This study aimed to determine the minimum bactericidal concentration (MBC) of fig leaf (F. carica Linn.) extract against E. faecalis and its cytotoxicity on fibroblast cells in vitro.

Materials and Methods: A serial dilution method was used to determine the MBC of fig leaf extract on E. faecalis which grown on nutrient agar media. Inoculation was carried out at concentrations that suspected minimum inhibitory concentration (MIC), MBC, concentration between MIC and MBC, and control groups on different nutrient agar. MIC and MBC of fig leaf extract against E. faecalis were known by counting the growth of bacteria colonies on nutrient agar media in CFU/ml. The cytotoxicity of MIC and MBC of the extract acquired were tested using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay, and the results were read using an ELISA reader. Data of E. faecalis colonies were analyzed using Kruskal-Wallis and Mann-Whitney test.

Results: The result showed a significant difference among the groups (p<0.05). Fig leaf extract at a concentration of 50% showed no bacterial growth, and cell viability at this concentration was 77.7%.

Conclusion: Fig leaf extract has antibacterial effect on E. faecalis with MBC at 50% and not cytotoxic to fibroblast cells.

Keywords: Enterococcus faecalis, fig leaf (Ficus carica Linn.) extract, minimum bactericidal concentration, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.

Status of theileriosis among herbivores in Iran: A systematic review and meta-analysis

Research (Published online: 19-03-2018)

13. Status of theileriosis among herbivores in Iran: A systematic review and meta-analysis

Masoud Soosaraei, Mousa Motavalli Haghi, Fariborz Etemadifar, Mahdi Fakhar, Saeed Hosseini Teshnizi, Hajar Ziaei Hezarjaribi and Shabnam Asfaram

Veterinary World, 11(3): 332-341

ABSTRACT

Aim: Theileriosis is a protozoal disease caused by Theileria spp. mostly in warm-blooded vertebrates worldwide. It is one of the common tick-borne diseases among domestic animals in tropical and sub-tropical regions, which have a variety of unlikable effects on health economy and animal welfare. In the present study, the prevalence of theileriosis among domestic farm animals in Iran was systematically evaluated.

Methods: To identify the related papers, 10 English and Persian databases, including PubMed, Science Direct, Scopus, Web of Science, Medical Subject Headings, Google Scholar, Magiran, Barakatns (formerly Iranmedex), Elm net, and Scientific Information Database, were appraised for articles published throughout 1999-2017.

Results: A total of 56 papers, providing the examination of 11,317 cattle, 9394 sheep, 2991 buffaloes, 1504 horses, 600 goats, and 212 donkeys were analyzed, matching for the prevalence of theileriosis from different parts of Iran were permitted for our allowing checklist. The overall prevalence of theileriosis among domestic herbivores was expected to be 19% (95% confidence interval: 15%, 22%). Our findings highlighted the average of the maximum prevalence in Razavi Khorasan (60.4%) and West Azerbaijan (49.1%) and the minimum in Mazandaran (1.1%) and East Azerbaijan provinces (2.2%), respectively. The high prevalence of Theileriainfection in the herbivores (mainly sheep) verifies the well-known enzootic episode of theileriosis in Iran, predominantly in northeastern and western parts of the country.

Conclusion: Our results suggested updated and imperative information on the true burden of theileriosis in Iran. Moreover, it could be supporting the gaps among monitoring, prevention, and control arrangements to improve the health economy, particularly among dairy farm animals.

Keywords: epidemiology, Iran, livestock, systematic review, Theileria spp.

Serum cortisol level and survival rate of juvenile Epinephelus fuscoguttatus following exposure to different salinities

Research (Published online: 17-03-2018)

12. Serum cortisol level and survival rate of juvenile Epinephelus fuscoguttatus following exposure to different salinities

Diyana Tahir, M. Shariff, Fadhil Syukri and F. M. Yusoff

Veterinary World, 11(3): 327-331

ABSTRACT

Background and Aim: Brown-marbled grouper Epinephelus fuscoguttatus is a premium marine food fish with high demand in Asia. In fish, stress due to environmental changes such as fluctuations in the salinity can result in increased cortisol level. Stress in fish increases susceptibility to diseases ultimately resulting in death. Therefore, the aim of this study was to investigate the salinity tolerance of E. fuscoguttatus and their survival in lower salinities.

Materials and Methods: In this study, grouper juveniles (92.43±standard error of the mean 0.51 mm) maintained in 31 ppt seawater were transferred into five tanks with seawater diluted to 25, 20, 15, 10, and 5 ppt. The salinity of the control group was not changed and was maintained at 31 ppt. Serum cortisol was measured using ELISA at 0, 30, 60, and 120 min after the fish were transferred to the different concentrations of salinity.

Results: The survival percentage was recorded for 14 days following the transfer and the results revealed that serum cortisol of fish in a high change in salinity (15, 10, and 5 ppt) was significantly higher than the control group immediately after exposure. At the high salinity change, the cortisol levels gradually decrease at 30 min and 60 min, until no difference in cortisol concentration was observed at 120 min. No mortality was observed in fish exposed to low salinity change (25 and 20 ppt) while in higher salinity change (5 ppt), the survival percentage was 50%.

Conclusion: The study revealed that the serum cortisol concentration was high initially and continues to decrease to resting cortisol level at 120 min indicating that cortisol hormone is released following acute stress as a primary response in grouper juveniles.

Keywords: cortisol, Epinephelus fuscoguttatus, salinity, serum, stress, survival.

The activity of pomegranate extract standardized 40% ellagic acid during the healing process of incision wounds in albino rats (Rattus norvegicus)

Research (Published online: 17-03-2018)

11. The activity of pomegranate extract standardized 40% ellagic acid during the healing process of incision wounds in albino rats (Rattus norvegicus)

Wiwik Misaco Yuniarti, Hardany Primarizky and Bambang Sektiari Lukiswanto

Veterinary World, 11(3): 321-326

Aim: This research aimed to evaluate the effects of pomegranate extract standardized to 40% ellagic acid on the incised wound in albino rats.

Materials and Methods: Fifty albino rats were divided into 10 treatment groups. The five groups were sacrificed on the 8th day, while the others were sacrificed on the 15th day. Two groups of albino rats with incised wound were not treated at all (P0), the other two groups of albino rats with incised wound were treated with Betadine® (P1) ointment, and the rest of the groups were treated with pomegranate extract standardized to 40% ellagic acid with a concentration of 2.5% (P2), 5% (P3), and 7.5% (P4). The treatments were carried out twice a day with an interval of 12 h for 7 and 14 days. At the end of the research, the skin tissue of those albino rats had been taken for histopathologic preparations before H and E staining was performed.

Results: Collagen deposition, polymorphonuclear neutrophils (PMN) infiltration, angiogenesis, and fibrosis degree in Group P4 treated with 7.5% pomegranate extract standardized to 40% ellagic acid for 14 days were significantly different from those in Groups P0, P1, P2, and P3, especially in the case of PMN inflammation (p<0.05).

Conclusion: The administration of 7.5% pomegranate extract standardized to 40% ellagic acid for 14 days on incised wounds of those albino rats can accelerate the wound healing process characterized by collagen deposition improvement, PMN infiltration in the wound area, angiogenesis, and fibrosis degree.

Keywords: albino rats, ellagic acid, incision wound, pomegranate, wound healing process.