A comprehensive study on seroprevalence of bluetongue virus in Haryana state of India

Research (Published online: 13-12-2017)

8. A comprehensive study on seroprevalence of bluetongue virus in Haryana state of India

Sushila Maan, Anuj Tiwari, Deepika Chaudhary, Anita Dalal, Nitish Bansal, Vinay Kumar, Kanisht Batra, Aman Kumar, Naresh Kumar Kakker and Narender Singh Maan

Veterinary World, 10(12): 1464-1470

ABSTRACT

Aim: The aim of present study was to determine seroprevalence of bluetongue virus (BTV) in Haryana state of India.

Materials and Methods: A total of 803 serum samples, 408 of cattle and 395 of buffalo origin, respectively, were collected from different villages of Haryana. Sampling was done randomly to obtain unbiased results. The samples were evaluated by a competitive enzyme-linked immunosorbent assay for the presence of BTV antibodies.

Results: Overall seroprevalence of BTV antibody in cattle and buffaloes for all 21 districts of Haryana state was found to be 75.49% and 92.91%, respectively. The prevalence of BTV in different agroclimatic zones ranged between 72-77% and 90-94% for cattle and buffalo, respectively. In buffaloes, the BTV seroprevalence was comparatively higher than in cattle.

Conclusion: The study showed that BTV is circulating in cattle and buffalo populations in the Northern part of India.

Keywords: bluetongue, bluetongue virus, buffalo, cattle, competitive enzyme-linked immunosorbent assay, Haryana, India, serology.

Effects of incorporating agro-industrial by-products into diet of New Zealand rabbits: Case of rebus of date and apricot kernel meal

Research (Published online: 12-12-2017)

7. Effects of incorporating agro-industrial by-products into diet of New Zealand rabbits: Case of rebus of date and apricot kernel meal

Achour Mennani, Rafik Arbouche, Yasmine Arbouche, Etienne Montaigne, Fodil Arbouche and Halima Saadia Arbouche

Veterinary World, 10(12): 1456-1463

ABSTRACT

Aim: The aim of this study was to determine the effects of incorporating the by-products complex of date and apricot on the fattening performance of the New Zealand breed of rabbits, to reduce the economic costs of the food formula.

Materials and Methods: A total of 288 young New Zealand rabbits aged 35 days were divided into four equal groups each containing 72 animals and into sub-groups of 6 rabbits per cage, depending on the rate of substitution of corn by date rebus and of soybean meal by apricot kernel meal (0%, 10%, 20%, and 30%).

Results: The change in weight from day 35 to 77 and the average daily gain are not significantly different, regardless of the diet. The pH and water content are proportional to the substitution rates (6.4-6.6% and 66.5-68.8%). Meat protein levels increased significantly, in particular for the 10% and 30% groups (+8.1% and 6%) while the fat and mineral content levels decreased significantly, in particular for the 30% group displaying -16% and -17%, respectively. Incorporation of dates and apricot kernel meal into the ration of rabbits reduces the cost of the kilogram of food produced of -9%, with an opportunity cost of 165 Algerian dinars (DZD).

Conclusion: The date rebus/apricot kernel meal complex can be used as an alternative to the corn/soybean meal complex at substitution rates of up to 30% without adverse effects on growth rates, feed contribution, or slaughter yield. It improves the chemical composition of the meat and reduces the cost price of the quintal of feed produced.

Keywords: agro-industrial by-product, apricot kernel meal, fattening, rabbits, rebus of dates.

Prophylactic and immune modulatory influences of Nigella sativa Linn. in broilers exposed to biological challenge

Research (Published online: 12-12-2017)

6. Prophylactic and immune modulatory influences of Nigella sativa Linn. in broilers exposed to biological challenge

Essam S. Soliman, Rania T. Hamad and Amira Ahmed

Veterinary World, 10(12): 1447-1455

ABSTRACT

Background and Aim: Prophylaxis and disease prevention is an essential strategy among biorisk management in poultry farms that stimulate and maintain the birds' immunity. The aim of this study was to investigate the prophylactic, and immune-stimulant influence of Nigella sativa Linn. in broilers under biological stress.

Materials and Methods: A total of 250 1-day-old (ross) chicks were divided into 5 groups; four of which were supplemented with 1.4%, 2.8%, 4.2%, and 5.6% N. sativa Linn., respectively. The four supplemented groups were challenged with Escherichia coli O₁₅₇:H₇ 1.5x10⁸ at a 14th day old. A total of 1050 samples (150 serum, 150 swab, and 750 organ samples) were collected and examined.

Results: A highly significant increase (p<0.01) in 5.6% N. sativa Linn. supplemented group in performance traits (body weight, weight gain, and performance index), biochemical parameters (proteinogram, liver enzymes, and creatinine), immunoglobulins concentration, and immune organs' weight. Meanwile, liver showed improvement of histoarchitecture without fibrosis. Heart showed a mild pericarditis with a mild degree of hydropic degeneration. Bursa, thymus, and spleen showed lymphoid hyperplasia.

Conclusion: A concentration of 5.6% N. sativa Linn. in broiler's feed can improve the immune response and subsequent resistance of broilers against diseases.

Keywords: Broiler, Escherichia coli, histopathology, Nigella sativa Linn., preventive.

Use of black soldier fly larvae (Hermetia illucens) to substitute soybean meal in ruminant diet: An in vitro rumen fermentation study

Research (Published online: 10-12-2017)

5. Use of black soldier fly larvae (Hermetia illucens) to substitute soybean meal in ruminant diet: An in vitro rumen fermentation study

Anuraga Jayanegara, Briliannanda Novandri, Nover Yantina and Muhammad Ridla

Veterinary World, 10(12): 1439-1446

ABSTRACT

Aim: This experiment aimed to evaluate substitution of soybean meal (SBM) by black soldier fly (BSF) larvae meal in a napier grass diet as performed by an in vitro rumen fermentation system.

Materials and Methods: Samples of napier grass, SBM, and BSF larvae age 1 week (BSF1) and 2 weeks (BSF2) were arranged according to the following dietary treatments (dry matter [DM] basis): T1, 100% napier grass; T2, 60% napier grass + 40% SBM; T3, 60% napier grass + 40% BSF1; T4, 60% napier grass + 40% BSF2; T5, 60% napier grass + 20% SBM + 20% BSF1; and T6, 60% napier grass + 20% SBM + 20% BSF2. The samples were determined for their chemical composition and were incubated in vitro using buffered rumen fluid for 48 h at 39°C. In vitro incubation was carried out in three runs and represented by two incubation bottles per run.

Results: Supplementation of BSF, both BSF1 and BSF2, increased ether extract, neutral- and acid-detergent insoluble crude protein contents of T3-T6 diets. The T3 or T4 diet resulted in lower ruminal ammonia concentration, in vitro DM digestibility, and in vitro organic matter (OM) digestibility as compared to those in T2 (p<0.05). Diet supplemented with BSF produced lower methane emission in comparison to that of supplemented with SBM (p<0.05). Diet containing BSF2 produced lower methane and methane per digestible OM than that containing BSF1 (p<0.05).

Conclusion: Substitution of SBM by BSF in ruminant diet results in a lower nutritional value in vitro but with an advantage of lowering ruminal methane emission.

Keywords: black soldier fly, chitin, insect, methanogenesis, rumen.

Cryptic etiopathological conditions of equine nervous system with special emphasis on viral diseases

Review (Published online: 10-12-2017)

4. Cryptic etiopathological conditions of equine nervous system with special emphasis on viral diseases

Rakesh Kumar and Rajendra D. Patil

Veterinary World, 10(12): 1427-1438

ABSTRACT

The importance of horse (Equus caballus) to equine practitioners and researchers cannot be ignored. An unevenly distributed population of equids harbors numerous diseases, which can affect horses of any age and breed. Among these, the affections of nervous system are potent reason for death and euthanasia in equids. Many episodes associated with the emergence of equine encephalitic conditions have also pose a threat to human population as well, which signifies their pathogenic zoonotic potential. Intensification of most of the arboviruses is associated with sophisticated interaction between vectors and hosts, which supports their transmission. The alphaviruses, bunyaviruses, and flaviviruses are the major implicated groups of viruses involved with equines/humans epizootic/epidemic. In recent years, many outbreaks of deadly zoonotic diseases such as Nipah virus, Hendra virus, and Japanese encephalitis in many parts of the globe addresses their alarming significance. The equine encephalitic viruses differ in their global distribution, transmission and main vector species involved, as discussed in this article. The current review summarizes the status, pathogenesis, pathology, and impact of equine neuro-invasive conditions of viral origin. A greater understanding of these aspects might be able to provide development of advances in neuro-protective strategies in equine population.

Keywords: bacterial diseases, seroprevalence, viral diseases.

Seroprevalence of viral and bacterial diseases among the bovines in Himachal Pradesh, India

Research (Published online: 03-12-2017)

3. Seroprevalence of viral and bacterial diseases among the bovines in Himachal Pradesh, India

Shailja Katoch, Shweta Dohru, Mandeep Sharma, Vikram Vashist, Rajesh Chahota, Prasenjit Dhar, Aneesh Thakur and Subhash Verma

Veterinary World, 10(12): 1421-1426

Aim: The study was designed to measure the seroprevalence of viral and bacterial diseases: Infectious bovine rhinotracheitis, bovine viral diarrhea, bovine leukemia, bovine parainfluenza, bovine respiratory syncytial disease, brucellosis, and paratuberculosis among bovine of Himachal Pradesh during the year 2013-2015.

Materials and Methods: The serum samples were collected from seven districts of state, namely, Bilaspur, Kangra, Kinnaur, Lahul and Spiti, Mandi, Sirmour, and Solan. The samples were screened using indirect ELISA kits to measure the seroprevalence of viral and bacterial diseases.

Results: The overall seroprevalence of infectious bovine rhinotracheitis was 24.24%, bovine viral diarrhea 1.52%, bovine leukemia 9.09%, bovine parainfluenza 57.58%, bovine respiratory syncytial disease 50%, brucellosis 19.69%, and paratuberculosis 9.09% in Himachal Pradesh. The seroprevalence of bovine rhinotracheitis, bovine leukemia, bovine parainfluenza, bovine respiratory syncytial disease, and paratuberculosis in the state varied significantly (p<0.01) while was insignificant for bovine viral diarrhea and brucellosis (p>0.01). Multiple seropositivity has been observed in this study. Bovine parainfluenza virus 3 was observed commonly in mixed infection with almost all viruses and bacteria under study.

Conclusion: The viral and bacterial diseases are prevalent in the seven districts of Himachal Pradesh investigated in the study. Therefore, appropriate management practices and routine vaccination programs should be adopted to reduce the prevalence of these diseases.

Keywords: bacterial diseases, seroprevalence, viral diseases.

Ocular squamous cell carcinoma in Holstein cows from the South of Brazil

Research (Published online: 01-12-2017)

2. Ocular squamous cell carcinoma in Holstein cows from the South of Brazil

Gabrielle A. Fornazari, Juliana Kravetz, Matti Kiupel, Dodd Sledge, Ivan Roque De Barros Filho and Fabiano Montiani-Ferreira

Veterinary World, 10(12): 1413-1420

ABSTRACT

Aim: The aim of this study was to investigate 10 cases of bovine ocular squamous cell carcinoma (OSCC) diagnosed in Holstein or Holstein-crosses cows.

Materials and Methods: The investigation was performed exclusively in OSCC cases diagnosed in the State of Parana and Santa Catarina. A combination of two previously existing histopathological classifications systems was used. The tissue samples were tested for immunoexpression of p53 and p16 and polymerase chain reaction (PCR) for bovine herpesvirus and papillomavirus.

Results: A positive correlation between number of mitotic figures and tissue invasion was found. Anaplasia parameters did not correlate well with tumor invasion of deeper tissues and mitotic counts. Six of 10 OSCC cases were in animals with heavily pigmented eyes. Immunoexpression of p53 and p16 was observed in 3 cases each. Bovine herpesvirus and papillomavirus were not detected by PCR.

Conclusion: Our results indicate that OSCC occurrence is most likely multifactorial with genetic, phenotypic, and environmental influences contributing to the pathogenesis of the disease.

Keywords: bovine, histologic classification, ophthalmology, p16, p53, tumor behavior.