Polymerase chain reaction detection of genes responsible for multiple antibiotic resistance Staphylococcus aureus isolated from food of animal origin in Egypt

Research (Published online: 09-10-2017)

8. Polymerase chain reaction detection of genes responsible for multiple antibiotic resistance Staphylococcus aureus isolated from food of animal origin in Egypt

Fawzy R. El Seedy, A. A. Samy, Hala S. H. Salam, Eman A. Khairy and Aya A. Koraney

Veterinary World, 10(10): 1205-1211

ABSTRACT

Aim: The aim of our study was polymerase chain reaction (PCR) detection of the genes responsible for the multiple antibiotic resistance S. aureus isolated from food of animal origin in Egypt.

Materials and Methods: A total of 125 samples were randomly collected from milk, meat, and their products from Giza and Beni-Suef Governorates markets. The S. aureus isolates were subjected to antimicrobial sensitivity tests using four antibacterial disks (Oxoid), and then the polymerase chain reaction (PCR) was performed for detection of antibiotic resistance genes.

Results: Out of 125 samples, 19 S. aureus isolates were detected. All detected isolates were multiple drug resistance (MDR). The penicillin-, erythromycin-, kanamycin-, and tetracycline-resistant isolates were examined by PCR for resistance genes blaZ, (msrA, ermB, and ermC), aac(6')aph (2''), and tetK. The isolates harbored these resistance genes with percentage of 100% (100%, 0%, and 100%), 62.5%, and 100%, respectively.

Conclusion: Contaminated foods of animal origin may represent a source of MDR S. aureus that can be a major threat to public health.

Keywords: food of animal origin, multiple antibiotic resistance, polymerase chain reaction, resistance genes, Staphylococcus aureus.

Public health concerns of highly pathogenic avian influenza H5N1 endemicity in Africa

Review (Published online: 08-10-2017)

7. Public health concerns of highly pathogenic avian influenza H5N1 endemicity in Africa

Olubunmi Gabriel Fasanmi, Ismail Ayoade Odetokun, Fatima Adeola Balogun and Folorunso Oludayo Fasina

Veterinary World, 10(10): 1194-1204

ABSTRACT

Highly pathogenic avian influenza virus (HPAIV) H5N1 was first officially reported in Africa in 2006; thereafter this virus has spread rapidly from Nigeria to 11 other African countries. This study was aimed at utilizing data from confirmed laboratory reports to carry out a qualitative evaluation of the factors responsible for HPAI H5N1 persistence in Africa and the public health implications; and to suggest appropriate control measures. Relevant publications were sought from data banks and repositories of FAO, OIE, WHO, and Google scholars. Substantiated data on HPAI H5N1 outbreaks in poultry in Africa and in humans across the world were mined. HPAI H5N1 affects poultry and human populations, with Egypt having highest human cases (346) globally. Nigeria had a reinfection from 2014 to 2015, with outbreaks in Cote d'Ivoire, Ghana, Niger, Nigeria, and Burkina Faso throughout 2016 unabated. The persistence of this virus in Africa is attributed to the survivability of HPAIV, ability to evolve other subtypes through genetic reassortment, poor biosecurity compliance at the live bird markets and poultry farms, husbandry methods and multispecies livestock farming, poultry vaccinations, and continuous shedding of HPAIV, transboundary transmission of HPAIV through poultry trades; and transcontinental migratory birds. There is, therefore, the need for African nations to realistically reassess their status, through regular surveillance and be transparent with HPAI H5N1 outbreak data. Also, it is important to have an understanding of HPAIV migration dynamics which will be helpful in epidemiological modeling, disease prevention, control and eradication measures.

Keywords: Africa, highly pathogenic avian influenza H5N1, public health implications.

Seroprevalence of Fasciola gigantica infection in bovines using cysteine proteinase dot enzyme-linked immunosorbent assay

Research (Published online: 06-10-2017)

6. Seroprevalence of Fasciola gigantica infection in bovines using cysteine proteinase dot enzyme-linked immunosorbent assay

Niranjan Kumar, Anju Varghese and J. B. Solanki

Veterinary World, 10(10): 1189-1193

ABSTRACT

Aim: The objective of the present study was to know the seroprevalence status of Fasciola gigantica infection in cattle and buffaloes using cysteine proteinase (CP) antigen in dot enzyme-linked immunosorbent assay (ELISA) format under field conditions.

Materials and Methods: As per the standard protocol, the sera were collected from the blood of 112 cattle and 38 buffaloes of coastal areas of Navsari district, South Gujarat, India. The indirect ELISA was performed on the strip of nitrocellulose paper blotted with 1 μl of CP antigen, to detect F. gigantica seropositive animals.

Results: The native CP of F. gigantica revealed a single visible band on 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. There was no any noted cross-reaction between the selected antigen and sera of Gastrothylax crumenifer-infected animals in ELISA. Out of 150 screened bovines, the sera of 47 (31.33%) were found to be reactive in dot-ELISA, with a prevalence rate of 31.25% and 31.58% in cattle and buffaloes, respectively. The seropositive bovines with heavy, moderate, and light level of infection were 44.68%, 34.04%, and 21.28%, respectively (p<0.05 between heavy and light; p>0.05 between moderate and heavy or light). The share of F. gigantica seropositive and negative animals was 31% and 69%, respectively. The optical density at 450 nm of pooled sera of seropositive bovines with heavy, moderate, and light reactivity in plate-ELISA was significantly higher with field or reference negative sera.

Conclusion: The CP-based dot-ELISA can be useful for field veterinarians for quick and timely isolation of the animals requiring urgent flukicide therapy.

Keywords: bovines, cysteine proteinase, dot enzyme-linked immunosorbent assay, Fasciola gigantica.

Effect of antioxidants supplementation on the quality of Beetal buck semen stored at 4°C

Research (Published online: 05-10-2017)

5. Effect of antioxidants supplementation on the quality of Beetal buck semen stored at 4°C

Archana Sarangi, Pardeep Singh, Meenakshi Virmani, A. S. Yadav, Subhasish Sahu, H. M. Ajithakumar, Anuradha Kumari and A. P. Rath

Veterinary World, 10(10): 1184-1188

ABSTRACT

Aim: An experiment was designed to evaluate the role of Vitamin E and glutathione in improving the seminal parameters during hypothermic storage of liquid semen at 4°C for 72 h.

Materials and Methods: Thirty-six semen ejaculates were collected by artificial vagina from 6 bucks (Beetal) during the normal reproduction season (September to November) at weekly interval. The samples were centrifuged, and the seminal plasma was removed. The sperm pellet was diluted with Tris-based extender and divided into three groups. Group T1: Control samples without antioxidants, Group T2: Samples supplemented with tocopherol at 3 mM, and Group T3: Samples supplemented with glutathione at 1 mM. The samples were evaluated for progressive motility, percent liveability, percent abnormal spermatozoa, and acrosome integrity after liquid preservation for 0, 24, 48, and 72 h. The level of lipid peroxidation and antioxidant enzymes, namely, glutathione peroxidase (GPx) and superoxide dismutase (SOD) were estimated after liquid preservation for 0 and 72 h.

Results: It was observed that, after storage of semen at 4°C up to 72 h, the progressive sperm motility, percent liveability, percent abnormal spermatozoa, and percent intact acrosomes were significantly (p<0.05) higher in group T2 and T3 samples as compared to control. However, the level of lipid peroxidation in T2 and T3 groups was significantly (p<0.05) lower after 72 h of incubation at 4°C. Similarly, GPx and SOD values were significantly (p<0.05) increased in T2 and T3 groups after 72 h of storage at 4°C.

Conclusion: Thus, it can be concluded that Vitamin E and glutathione supplementation at 3 mM and 1 mM, respectively, while preserving the semen samples at 4°C helped in maintaining the seminal parameters up to 72 h and protected the spermatozoa from oxidative damage.

Keywords: Beetal buck, glutathione and liquid preservation, oxidative stress, semen, seminal parameters, Vitamin E.

Analysis of spatial dynamic of epizootic process of bluetongue and its risk factors

Research (Published online: 04-10-2017)

4. Analysis of spatial dynamic of epizootic process of bluetongue and its risk factors

Fayssal Bouchemla, Olga Mikhailovna Popova and Valerey Alexandrovich Agoltsov

Veterinary World, 10(10): 1173-1183

ABSTRACT

Aim: The study was undertaken to find out the spatial dynamic occurrence and patterns of the global spread of bluetongue (BT) disease for the period from 1996 to 2016, as well as the assessment of the risk of occurrence and its spread in 2017-2018.

Materials and Methods: Outbreaks (serum samples were collected from clinically healthy as well as suspected animals in infected points) were confirmed and reported officially by veterinary departments which represent different geographical regions in the world to World Organization for Animal Health. These reports explained that ELISA and polymerase chain reaction were used to identify the BT disease, taking in the account number of infected, dead animals, and focus of BT infection in all susceptible animals from 1996 to 2016. Once conventional statistical population was defined (an observational study), we had classified data as well as possible to answer to our aim, using descriptive statistics methods, including the test of the relationship between different epizootiological indicators.

Results: The spatial dynamic study of BT's occurrence and its spread in the world over the two past decades was presented by different epizootic indicators. The given analysis includes assessment and measurement of risk factors. It was built too, regression models, and allowed to put different forecasts on the different epizootic indicators in the years 2017-2018 by the extrapolation method. We had also determined that, in 2017, BT continues to spread with the total expectancy of 3.4 focus of infection (number of diseased animals in a single unfavorable point) and mortality of about 26 %; these rates tend to decrease in 2018. At abused points by BT, up to 78.4% of animals are mixed (more than one type) and in 21.6% - uniform. By this way, the relative risk of the incidence of appearance-abused points in mixed households has 3.64, which might be considered higher for the BT dissemination. Moreover, between the enzootic index and other epizootiological indicators had revealed an inverse correlation, i.e., to an increase in the level of enzootic index among the cattle population would be formed population less sensitive to BT. Cluster analysis was done, which had demonstrated the zoning of risk levels in the world and the occurrence of the disease intensity in the period 1996-2016 years. Then, assess connection degree of the dynamic of BT tension with geographical and socioeconomic conditions background 0.66 and 0.68, respectively.

Conclusion: It is important to define a variety of BT risk factors and assess their influence on BT occurrence. However, the most important is to define the overlapping coinfluence between them that cause serious losses. To have an out of BT territory needs to make an emphasis of co-influence of risk factors on this zone. Was predicted a continue hits of disease in the next year with weight moderation through one year. Far from statists, to assess the given forecast may have a serious variety, taken in account problems of actual climate change in the world.

Keywords: bluetongue, extrapolation, outbreaks, regression model, risk factors.

Phenotypic and genotypic characterization of antimicrobial susceptibility of avian pathogenic Escherichia coli isolated from broiler chickens

Research (Published online: 03-10-2017)

3. Phenotypic and genotypic characterization of antimicrobial susceptibility of avian pathogenic Escherichia coli isolated from broiler chickens

Gamal Younis, Amal Awad and Nada Mohamed

Veterinary World, 10(10): 1167-1172

ABSTRACT

Aim: Avian pathogenic Escherichia coli (APEC) is pathogenic strains of E. coli that are responsible for one of the most common bacterial diseases affecting poultry worldwide. This study was designed to determine the occurrence, antibiotic resistance profile, and antibiotic resistance genes of E. coli isolated from diseased and freshly dead broilers.

Materials and Methods: In that context, a total of 200 broilers samples were examined by standard microbiological techniques for isolation of E. coli, and tested for their antimicrobial susceptibility against 15 antimicrobial agents using disc diffusion method. In addition, E. coli isolates were screened by multiplex polymerase chain reaction for detection of a number of resistance genes including aadA1 gene encodes streptomycin/neomycin, tetA encodes resistance to tetracycline, sul1 encodes sulfonamides, and β-lactamase encoding genes (blaTEM and blaSHV).

Results: A total of 73 (36.5%) isolates were biochemically identified as E. coli strains. O78, O2, and O1 are the most prevalent serotypes detected. E. coli displayed a high resistance against penicillin (100%), followed by cefepime (95.8%) and a low resistance to norfloxacin (36.9%), and chloramphenicol (30%). Depending on the results of PCR, sul1 gene was the most predominant antibiotic resistant gene (87%) followed by blaTEM (78%), tetA genes (60%), and aadA (54%). However, blaSHV had the lowest prevalence (23%).

Conclusion: The obtained results demonstrated the importance of studies on APEC and antibiotic resistance genes in our region which associated with intensive poultry industry, aiming to acquire preventive measures to minimize losses due to APEC and associated multidrug-resistance and resistance genes that of high significance to the rational use of antibiotics in clinical and public health.

Keywords: antimicrobial resistance, broilers, Escherichia coli, multiplex polymerase chain reaction, resistant genes.

Serological investigation of some important RNA viruses affecting sheep and goats in Giza and Beni-Suef governorates in Egypt

Research (Published online: 02-10-2017)

2. Serological investigation of some important RNA viruses affecting sheep and goats in Giza and Beni-Suef governorates in Egypt

Mohamed Abd El-Fatah Mahmoud, Mohamed Karam Elbayoumy, Doaa Sedky and Sahar Ahmed

Veterinary World, 10(10): 1161-1166

ABSTRACT

Aim: The aim of this study was to investigate the seroprevalence of antibodies against foot and mouth disease (FMD), Peste des Petits ruminants (PPR), and bluetongue (BT) in sheep and goats within Giza and Beni-Suef governorates at the second half of 2016.

Materials and Methods: A total of 300 animals (sheep and goats) randomly selected from small stocks with no history of previous vaccination against FMD virus (FMDV), PPR, or BT viruses (BTV) and examined with competitive enzyme-linked immunosorbent assay for detection of FMD-non-structural protein, PPR, and BT antibodies.

Results: Seroprevalence analysis revealed that antibodies against FMDV were 40.8% and 37.1% at Giza governorate, while at Beni-Suef governorate, the percent was 36.7% and 50% in sheep and goat, respectively. Antibodies against PPR were 63.8% in sheep and 45.7% in goats at Giza governorate, whereas the results for Beni-Suef governorate were 71.7% in sheep and 45% in goats. Antibodies against BT were 45% and 37% in sheep and goats, respectively, in Giza governorate, whereas the results for Beni-Suef governorate were 80% and 55% in sheep and goats, respectively. The average of BTV antibody prevalence was significantly higher in sheep (45% and 80%) than in goats (37% and 55%) in Giza and Beni-Suef, respectively. Statistical analysis for the three viruses showed the high relation between the two governorates in case of sheep (r=0.85) and in case of goats (r=0.87). In general, a strong positive correlation was observed between the governorates (r=0.93).

Conclusion: Giza and Beni-Suef governorates are endemic with FMDV, PPR, and BTV. Regional plan for characterization and combating FMD, PPR, and BT is recommended to help in the achievement of the most suitable combination of the vaccine regimen.

Keywords: bluetongue, enzyme-linked immunosorbent assay, foot and mouth disease, goats, Peste des Petits ruminants, sheep.