Pathology and immunohistochemistry study of Newcastle disease field case in chicken in Indonesia

Research (Published online: 13-09-2017)

10. Pathology and immunohistochemistry study of Newcastle disease field case in chicken in Indonesia

Etriwati, Dewi Ratih, Ekowati Handharyani and Surachmi Setiyaningsih

Veterinary World, 10(9): 1066-1071

ABSTRACT

Aim: The aim of the study was to examine pathology and the distribution pattern of Newcastle disease virus (NDV) in internal organs of chickens from a field case using immunohistochemical staining.

Materials and Methods: 10 groups of broiler, layer, and domestic chicken were collected from necropsy room Division of Pathology, Bogor Agricultural University. These chickens were originated from West Java and collected based on pathologist diagnosis as suspect of Newcastle disease (ND). They were subsequently confirmed positive of ND with real-time-reverse transcription polymerase chain reaction assay. The respiratory, circulatory, digestive, lymphoreticular and central nervous systems were collected for histopathology examination.

Results: The gross pathology and histopathology changes were tracheitis, pneumonia, pericarditis, myocarditis, catarrhal proventriculitis, catarrhal enteritis, typhlitis, perihepatitis, pancreatitis, nephritis interstitial, splenitis, atrophy of Bursa Fabricius, and encephalitis.

Conclusion: The distribution pattern of NDV in internal organs of chickens from a field case in this study is similar with a previous reported pattern in systemic cases of the internal chicken organs. High intensity of immunohistochemistry stain result was detected in trachea, lung, proventriculus, duodenum, cecal tonsil, kidney, and brain.

Keywords: broiler, domestic chicken, immunohistochemistry, layer, Newcastle disease.

Detection of anti-Toxoplasma gondii antibodies in chronic myeloid leukemia and acute myeloid leukemia patients

Research (Published online: 13-09-2017)

9. Detection of anti-Toxoplasma gondii antibodies in chronic myeloid leukemia and acute myeloid leukemia patients

Mohammad Javad Gharavi, Mona Roozbehani and Zienat Mandeh

Veterinary World, 10(9): 1063-1065

ABSTRACT

Background and Aim: Infection of Toxoplasma gondii is a worldwide distribution. Toxoplasmosis in patients who are immunocompromised by virtue of underlying leukemia disease has received relatively little attention. This study was aimed to evaluate IgG and IgM antibodies of T. gondii and to minimize the role of T. gondii and opportunistic infection complication at the early stage of infection in leukemia patients.

Materials and Methods: The purpose of this assay was to measure anti-T. gondii IgG and IgM antibodies by enzyme-linked immunosorbent assay (ELISA) technique in leukemia patients.

Results: IgG antibodies against T. gondii were detected by ELISA in 96 (56.4%) leukemia patients and 72 (42.4%) control group. IgM antibodies were found in 10 patients (5.9%) with leukemia and 3 (1.8%) in the corresponding.

Conclusion: Our finding indicated that leukemia patients under immunosuppressive condition should not be neglected. Toxoplasmosis in leukemia patients as a main risk factor is considered, meanwhile in some patients, due to possibility of the presence of secondary infection that leads to severe toxoplasmosis.

Keywords: acute myeloid leukemia, chronic myeloid leukemia, Toxoplasma gondii.

Mastitis vaccines in dairy cows: Recent developments and recommendations of application

Review (Published online: 12-09-2017)

8. Mastitis vaccines in dairy cows: Recent developments and recommendations of application

Zuhair Bani Ismail

Veterinary World, 10(9): 1057-1062

ABSTRACT

The objective of this review article was to summarize the most recent clinical field trials that have been published evaluating the use of different types of vaccines against mastitis pathogens in dairy cows. Mastitis is one of the most common and economically important diseases in dairy cows in the world. The disease is considered an important welfare issue facing the dairy industry in addition to the loss of production and premature removal or death of affected cows. Losses are also related to high cost of veterinary medicines and the cost of unsalable milk of treated cows. Mastitis can be caused by either contagious or environmental pathogens both of which are best prevented rather than treated. In addition to the application of best management practices in the parlor during milking, vaccination against common udder pathogens is widely practiced in many dairy farms to prevent or reduce the severity of clinical mastitis. In this review, the most recent clinical field studies that evaluated the use of different types of vaccines in dairy cows are summarized.

Keywords: dairy cows, immunization, mastitis, pathogens, udder.

Application of radio frequency based digital thermometer for real-time monitoring of dairy cattle rectal temperature

Research (Published online: 12-09-2017)

7. Application of radio frequency based digital thermometer for real-time monitoring of dairy cattle rectal temperature

Tridib Debnath, Santanu Bera, Suman Deb, Prasenjit Pal, Nibash Debbarma and Avijit Haldar

Veterinary World, 10(9): 1052-1056

ABSTRACT

Aim: Dairy cattle health monitoring program becomes vital for detecting the febrile conditions to prevent the outbreak of the animal diseases as well as ensuring the fitness of the animals that are directly affecting the health of the consumers. The aim of this study was to validate real-time rectal temperature (RT) data of radio frequency based digital (RFD) thermometer with RT data of mercury bulb (MB) thermometer in dairy cattle.

Materials and Methods: Two experiments were conducted. In experiment I, six female Jersey crossbred cattle with a mean (±standard error of the mean) body weight of 534.83±13.90 kg at the age of 12±0.52 years were used to record RT for 2 h on empty stomach and 2 h after feeding at 0, 30, 60, 90, and 120 min using a RFD thermometer as well as a MB thermometer. In experiment II, six female Jersey crossbred cattle were further used to record RT for 2 h before exercise and 2 h after exercise at 0, 30, 60, 90, and 120 min. Two-way repeated measures analysis of variance with post hoc comparisons by Bonferroni test was done.

Results: Real-time RT data recorded by RFD thermometer as well as MB thermometer did not differ (p>0.05) before and after feeding/exercise. An increase (p<0.05) in RT after feeding/exercise in experimental crossbred cattle was recorded by both RFD thermometer and MB thermometer.

Conclusion: The results obtained in the present study suggest that the body temperature recordings from RFD thermometer would be acceptable and thus RFD thermometer could work well for monitoring real-time RT in cattle.

Keywords: cattle, exercise, feeding, radio frequency device, rectal temperature, thermometer.

Hydrophilic nanosilica as a new larvicidal and molluscicidal agent for controlling of major infectious diseases in Egypt

Research (Published online: 11-09-2017)

6. Hydrophilic nanosilica as a new larvicidal and molluscicidal agent for controlling of major infectious diseases in Egypt

Marwa M. Attia, Soliman M. Soliman and Mahmoud A. Khalf

Veterinary World, 10(9): 1046-1051

ABSTRACT

Aim: This research was conducted to evaluate the molluscicidal and mosquitocidal efficacy of silica nanoparticles in the eradication of the larvae and pupa of malaria and filariasis vector as well as vectors of rift-valley fever virus (Culex pipiens); Schistosoma mansoni vector (Biomphlaria alexandrina (snail and egg masses)).

Materials and Methods: Hydrophilic nanosilica particles (NSPs) were characterized using transmission electron microscope during the preliminary part of the study; the stages were exposed to upgrade concentrations of NSP from 50 to 1200 ppm each for 24-36 h exposure time. The highly effective concentrations were re-evaluated at lower exposure time as 3, 6, and 12 h.

Results: Lethal concentration (LC₅₀) and LC₉₀ versus mosquito larvae were (350 ppm/24 h and 1400 ppm/24 h, respectively). C. pipiens pupae proved slight high tolerance versus the effect of these nanoparticles as the two previous doses increased to 680 ppm/6 h and 1300 ppm/24 h. The LC₅₀ and LC₉₀ versus B. alexandrina were increased to 590 ppm/6 h and 980 ppm/48 h, respectively. Moreover, the embryonated snail egg masses appear more susceptible to the toxic effect of these nanoparticles than the non-embryonated eggs as the LC₅₀ and LC₉₀ were increased to 1450 ppm/12 h and 1250 ppm/48 h, respectively, for embryonated eggs, and it was 1400 ppm/24 h and 1890 ppm/48 h, respectively, for non-embryonated one.

Conclusion: The results open a new field for controlling the infectious diseases through eradication of their vectors by the way that avoids the resistance recorded from the successive chemical application in this field.

Keywords: Biomphalaria alexandrina, Culex pipiens, Egypt, nanosilica, rift valley fever, schistosomiasis.

Detection of food-borne bacteria in ready to eat betel leaf sold at local markets in Mymensingh

Research (Published online: 11-09-2017)

5. Detection of food-borne bacteria in ready to eat betel leaf sold at local markets in Mymensingh

Md. Mazedul Haque, Md. Atiqur Rahman Sarker, Rafia Afroze Rifa, Md. Ariful Islam and Mst. Minara Khatun

Veterinary World, 10(9): 1040-1045

ABSTRACT

Aim: The present study was undertaken to determine bacterial load as well as characterize bacterial flora of ready to eat (RTE) betel leaf sold at local markets in Mymensingh city.

Materials and Methods: A total of 25 RTE betel leaf samples were collected from five local markets such as Kamal-Ranjit (KR) market, Shesh more, Kewatkhali, Jobber more, and Ganginar par.

Results: Total viable count of bacteria in betel leaf (log10 mean colony forming unit±standard deviation/ml) was 7.58±0.04 for KR market, 7.72±0.06 for Shesh more, 7.62±0.04 for Kewatkhali, 7.40±0.03 for Jobber more, and 7.60±0.06 for Ganginar par. A total of 98 bacterial isolates belong to five genera (Escherichia coli, Salmonella spp., Vibrio spp., Bacillus spp., and Staphylococcus spp.) were identified. The prevalence of E. coli was 17.34%, Salmonella spp. was 25.51%, Vibrio spp. was 19.39%, Bacillus spp. was 18.37%, and Staphylococcus spp. was 19.39%. Antibiotic sensitivity test showed that all isolates were sensitive to two antibiotics such as ciprofloxacin and gentamicin. Four isolates (E. coli, Salmonella spp., Vibrio spp., and Staphylococcus spp.) were resistant to two antibiotics (ampicillin and cephalexin). Antibiogram profile of bacterial isolates of betel leaf suggests that they were multidrug resistance.

Conclusion: Data of this study indicate that betel leaf sold at local market harbors multidrug resistance food-borne bacteria which might cause public health hazards if these antibiotic resistant transfer to human through food chain.

Keywords: betel leaf, food-borne bacteria, Mymensingh city, public health importance.

Detection of Toxoplasma gondii in cat's internal organs by immunohistochemistry methods labeled with-[strept] avidin-biotin

Research (Published online: 06-09-2017)

4. Detection of Toxoplasma gondii in cat's internal organs by immunohistochemistry methods labeled with-[strept] avidin-biotin

Muhammad Hanafiah, Raden Wisnu Nurcahyo, Rika Yuniar Siregar, Joko Prastowo, Sri Hartati, Bambang Sutrisno and Dwinna Aliza

Veterinary World, 10(9): 1035-1039

ABSTRACT

Aim: The aims of the study are to detect the presence of Toxoplasma gondii antigen and to determine its distribution location in several organs of domestic cat using immunohistochemistry (IHC) method with Labeled-[Strept] Avidin-Biotin (LAB-SA).

Materials and Methods: Four domestic cats aged 1-2 years were used as sample in this research. The sample divided into two groups with two cats each. Cats in Group I were positive Toxoplasma based on serologically screening test, while cats in Group II were orally infected with 1x106 Toxoplasma oocyst. All samples then necropsied, and the organs including brain, liver, kidney, duodenum, jejunum, ileum, lungs, and spleen were collected for IHC method with LAB-SA.

Result: The result showed that Toxoplasma antigens were detected in ileum of both serologically positive domestic cat and the experimentally infected cats. Toxoplasma was also observed in kidney of serologically positive domestic cat. In the serologically positive domestic cat, necrotic lesions were found on ileum, kidney, and liver, whereas in experimentally infected cat, the lesion was only found on ileum.

Conclusion: The presence of Toxoplasma antigen is successfully detected in several organs of domestic cat using IHC method with the LAB-SA.

Keywords: cat, immunohistochemistry, labeled-[strept] avidin-biotin, Toxoplasma gondii.