Preparation and evaluation of Salmonella Enteritidis antigen conjugated with nanogold for screening of poultry flocks

Research (Published online: 02-08-2017)

2. Preparation and evaluation of Salmonella Enteritidis antigen conjugated with nanogold for screening of poultry flocks

Hazem Mohammed Ibrahim, Rafik Hamed Sayed, Wafaa Ragab Abdel-Aziz and Rafik Tawfik Soliman

Veterinary World, 10(8): 848-853

ABSTRACT

Aim: The present work aimed to develop lateral flow immunochromatographic strip (ICS) test for detection of Salmonella Enteritidis (SE) specific antibodies in chicken sera.

Materials and Methods: A rapid lateral flow immunochromatographic test (LFIT) has been developed, in which SE Group D antigen labeled with the gold chloride molecules laid on the conjugate pad. Staphylococcus aureus protein A was used as capture antibody at the test line (T) of a nitrocellulose (NC) membrane and anti-SE antigen-specific rabbit antibodies were used as capture antibody at the control line (C) of the NC strip in the lateral flow layout device.

Results: Using the developed LFIT, the minimal amount of SE-specific antibodies that can be detected in chicken serum sample was 1427 enzyme-linked immunosorbent assay (ELISA) unit/100 μl that was equal to 0.1 μg (Ab)/100 μl sample. 100 suspected serum samples collected from a poultry flock were tested with the prepared SE-LFIT kits and the locally prepared stained Salmonella antigen, and the results were compared with those obtained from examination of these samples with Salmonella Group D antibody ELISA kit as the gold standard test. The sensitivity, specificity, and accuracy of the prepared SE-LFIT antigen kits were 94.4%, 90%, and 94%, respectively, while those obtained with stained Salmonella antigen were 88.8%, 90%, and 89%, respectively.

Conclusion: The developed test is a simple field rapid test of high sensitivity, specificity, and accuracy that can improve and facilitates rapid field surveillance of salmonellosis among chickens.

Keywords: diagnosis, lateral flow, poultry, Salmonella Enteritidis.

Detection of Staphylococcus aureus enterotoxigenic strains in bovine raw milk by reversed passive latex agglutination and multiplex polymerase chain reaction

Research (Published online: 01-08-2017)

1. Detection of Staphylococcus aureus enterotoxigenic strains in bovine raw milk by reversed passive latex agglutination and multiplex polymerase chain reaction

Asmaa Samy Mansour, Gad El-Said Wagih, Sabry D. Morgan, Mahmoud Elhariri, Mona A. El-Shabrawy, Azza S. M. Abuelnaga and E. A. Elgabry

Veterinary World, 10(8): 843-847

ABSTRACT

Aim: This review gives an outline of the assessment of enterotoxigenic Staphylococcus aureus tainting levels in raw milk from different sources in Egypt and characterization of enterotoxigenic strains utilizing a technique in light of PCR to identify genes coding for the production of staphylococcal enterotoxin (SE). The obtained data were compared with results from the application of the reversed passive latex.

Materials and Methods: Multiplex PCR and reversed passive latex agglutination (RPLA) were used. A total of 141 samples of raw milk (cow's milk=33, buffalo's milk=58, and bulk tank milk=50) were investigated for S. aureus contamination and tested for enterotoxin genes presence and toxin production.

Results: S. aureus was detected in 23 (16.3%) samples phenotypically and genotypically by amplification of nuc gene. The S. aureus isolates were investigated for SEs genes (sea to see) by multiplex PCR and the toxin production by these isolates was screened by RPLA. SEs genes were detected in six isolates (26.1%) molecularly; see was the most observed gene where detected in all isolates, two isolates harbored seb, and two isolates harbored sec. According to RPLA, three isolates produced SEB and SEC.

Conclusion: The study revealed the widespread of S. aureus strains caring genes coding for toxins. The real significance of the presence of these strains or its toxins in raw milk and their possible impact a potential hazard for staphylococcal food poisoning by raw milk consumption. Therefore, detection of enterotoxigenic S. aureus strains in raw milk is necessary for consumer safety.

Keywords: enterotoxin genes, multiplex polymerase chain reaction, reversed passive latex agglutination, raw milk, S. aureus.

Serogrouping and antibiotic resistance of Escherichia coli isolated from broiler chicken with colibacillosis in center of Algeria

Research (Published online: 29-07-2017)

21. Serogrouping and antibiotic resistance of Escherichia coli isolated from broiler chicken with colibacillosis in center of Algeria

Zehor Halfaoui, Nabil Mohamed Menoueri and Lyes Mohamed Bendali

Veterinary World, 10(7): 830-835

ABSTRACT

Aim: Colibacillosis is considered as one of the major bacterial infections in avian pathology. The excessive use of antibiotics reduced their effectiveness, which eventually led to the risk of emergence of antibiotic resistance. The aim of this study was to isolate, identify and serotype the pathogenic Escherichia coli strains and to determine their antibiotic susceptibility.

Materials and Methods: A total of 180 samples from different organs of broilers with colibacillosis lesions were collected (liver, spleen, lung, and heart) in center of Algeria. The isolation and identification of E. coliwere carried out using conventional techniques. Then, these strains were serotyped and tested over 13 antibiotics.

Results: A total of 156 strains of E. coli were isolated. Serotyping results showed that 50 strains belong to 3 serotypes (23 for O1, 11 for O2, 16 for O78) which represent 32% of isolates. The antimicrobial susceptibility test, presented high level of resistance to tetracyclines (94.12%), flumequine (91.5%), sulfamethoxazole-trimethoprim (88.89%), enrofloxacin (86.27%), nalidixic acid (85.62%), ampicillin (83.01%) and doxycycline (75.81%), medium level resistance to chloramphenicol (39.22%), and amoxicillin-clavulanic acid (43.13%). All the strains were susceptible to cefotaxime, excepting three, which presented an extended spectrum β-lactamase (ESBL). In addition, the results of multi-resistance showed that all the strains were resistant at the minimum to two antibiotics and 66.66% of strains were resistant to at least seven antibiotics.

Conclusion: The antibiotic resistance continues to rise at an alarming rate, and the emergence of ESBL is considered as a threat for public health.

Keywords: Algeria, antibiotic resistance, broilers, colibacillosis, Escherichia coli, serogrouping.

Probiotic characterization of lactic acid bacteria isolated from swine intestine

Research (Published online: 28-07-2017)

20. Probiotic characterization of lactic acid bacteria isolated from swine intestine

K. Balasingham, C. Valli, L. Radhakrishnan and D. Balasuramanyam

Veterinary World, 10(7): 825-829

ABSTRACT

Aim: A study was conducted with the objective to isolate probiotic microorganisms from swine intestine.

Materials and Methods: In this study 63 isolates (24 caeca, 24 colon mucosal scrapings, and 15 rectal swab samples) were collected from Large White Yorkshire pigs. The isolates were inoculated and grown in de Man Rogosa Sharpe broth at 37°C with 5% CO2 for 48 h and subjected to morphological identification. Colonies having Gram-positive rods were selected for further physiological and biochemical identification tests, which were conducted in triplicate in two runs for each of the selected isolates using a standard protocol. Probiotic properties among the identified species were determined through the implementation of several tests related with pH tolerance, bile tolerance, and antimicrobial activity.

Results: Morphological identification revealed that only 23 isolates were Gram-positive rods. Physiological tests performed on these 23 isolates further revealed that four of them did not exhibit any growth, at all conditions studied. The rest 19 isolates were, therefore, selected and subjected to biochemical tests. Six isolates were rejected because they were oxidase and nitrate reduction positive. From the 13 isolates subjected to sugar fermentation tests, speciation of only two isolates could be ascertained, one of the isolates showed characteristics for Lactobacillus acidophilus and the other for Lactobacillus plantarum. These two isolates were assessed for the strain possessing maximum probiotic property, and it was inferred that both - L. plantarum and L. acidophilus could tolerate a wide pH range (2-9), a wide bile concentration (0.05-0.3%) and revealed antimicrobial activity toward Escherichia coli, and Enterobacter spp.

Conclusion: L. plantarum and L. acidophilus were isolated from swine intestine and were found to have good probiotic properties.

Keywords: Lactobacillus acidophilus, Lactobacillus plantarum, probiotic, swine intestine.

Methicillin-resistant Staphylococcus aureus among patients in a teaching hospital in Ghana

Research (Published online: 25-07-2017)

8. Methicillin-resistant Staphylococcus aureus among patients in a teaching hospital in Ghana - Akosua Bonsu Karikari, Enoch Frimpong and Alex Owusu-Ofori

International Journal of One Health, 3: 46-49

doi: 10.14202/IJOH.2017.46-49

Abstract

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Aim: This study determined the prevalence of methicillin resistant Staphylococcus aureus (MRSA) and assessed the resistance profiles of strains.

Materials and Methods: Inpatients and outpatients of all age groups presenting with sepsis as well as skin and soft tissue infections were screened from October 2006 to March 2007. Resistance to methicillin (oxacillin) and other relevant antibiotics was determined by the Kirby-Bauer disk diffusion and minimum inhibitory concentration (MICs) by the E-test (AB, Biodisk, Solna, Sweden).

Results: Methicillin resistance was 34.8% (87/250), majority (67/87) of which were hospital acquired MRSA. Resistance was 100% to the β-lactams, 78.2% to cotrimoxazole, 75.8% to tetracycline, 59.8% to gentamicin, 56.3% to flucloxacillin, 34.4% to erythromycin, and 32.2% to cefuroxime. MIC ranged from 4-256, 0.125-256, 0.064-32, and 1.5-32, respectively, to oxacillin, gentamicin, cotrimoxazole, and ceftriaxone.

Conclusion: Prevalence of MRSA is high in Komfo Anokye Teaching Hospital, and routine surveillance should be put in place to monitor the epidemiology of this pathogen.

Keywords: antibiotic resistance, Ghana, Komfo Anokye Teaching Hospital, methicillin-resistantStaphylococcus aureus.

Baseline hematology and serum biochemistry results for Indian leopards (Panthera pardus fusca)

Research (Published online: 24-07-2017)

19. Baseline hematology and serum biochemistry results for Indian leopards (Panthera pardus fusca)

Arun Attur Shanmugam, Sanath Krishna Muliya, Ajay Deshmukh, Sujay Suresh, Anukul Nath, Pa Kalaignan, Manjunath Venkataravanappa and Lyju Jose

Veterinary World, 10(7): 818-824

ABSTRACT

Aim: The aim of the study was to establish the baseline hematology and serum biochemistry values for Indian leopards (Panthera pardus fusca), and to assess the possible variations in these parameters based on age and gender.

Materials and Methods: Hemato-biochemical test reports from a total of 83 healthy leopards, carried out as part of routine health evaluation in Bannerghatta Biological Park and Manikdoh Leopard Rescue Center, were used to establish baseline hematology and serum biochemistry parameters for the subspecies. The hematological parameters considered for the analysis included hemoglobin (Hb), packed cell volume, total erythrocyte count (TEC), total leukocyte count (TLC), mean corpuscular volume (MCV), mean corpuscular Hb (MCH), and MCH concentration. The serum biochemistry parameters considered included total protein (TP), albumin, globulin, aspartate aminotransferase, alanine aminotransferase (ALT), blood urea nitrogen, creatinine, triglycerides, calcium, and phosphorus.

Results: Even though few differences were observed in hematologic and biochemistry values between male and female Indian leopards, the differences were statistically not significant. Effects of age, however, were evident in relation to many hematologic and biochemical parameters. Sub-adults had significantly greater values for Hb, TEC, and TLC compared to adults and geriatric group, whereas they had significantly lower MCV and MCH compared to adults and geriatric group. Among, serum biochemistry parameters the sub-adult age group was observed to have significantly lower values for TP and ALT than adult and geriatric leopards.

Conclusion: The study provides a comprehensive analysis of hematologic and biochemical parameters for Indian leopards. Baselines established here will permit better captive management of the subspecies, serve as a guide to assess the health and physiological status of the free ranging leopards, and may contribute valuable information for making effective management decisions during translocation or rehabilitation process.

Keywords: hematology, Indian leopard, Panthera pardus fusca, serum biochemistry.

Characterization of Salmonella Gallinarum isolates from backyard poultry by polymerase chain reaction detection of invasion (invA) and Salmonella plasmid virulence (spvC) genes

Research (Published online: 23-07-2017)

18. Characterization of Salmonella Gallinarum isolates from backyard poultry by polymerase chain reaction detection of invasion (invA) and Salmonella plasmid virulence (spvC) genes

Susmita Pal, Samir Dey, Kunal Batabyal, Abhiroop Banerjee, Siddhartha Narayan Joardar, Indranil Samanta and Devi Prasad Isore

Veterinary World, 10(7): 814-817

ABSTRACT

Aim: The aim was to characterize Salmonella enterica serovar Gallinarum isolated from backyard poultry by polymerase chain reaction (PCR) detection of virulence genes invasion (invA) and Salmonella plasmid virulence C (spvC).

Materials and Methods: Two strains of Salmonella serovar Gallinarum isolates used in this study were obtained from an outbreak of fowl typhoid in backyard Vanaraja fowl. PCR technique was used for detection of invA and spvC genes using standard methodology. The invA PCR product from one representative isolate was sequenced and compared with other related Salmonella serovars in GenBank data.

Results: Salmonella Gallinarum produced expected amplicons of invA and spvC gene products. Nucleotide sequence of 285 bp invA gene was deposited in GenBank with accession no. KX788214. Sequence analysis of invA gene was found conserved in Salmonella serovars and demonstrated 100% homology with closely related serovars of Salmonella.

Conclusion: Invasion gene (invA) was found to be highly conserved in Salmonella Gallinarum and highly similar with closely related serovars. The isolates also contained plasmid-mediated spvC gene indicating possession of virulence plasmid.

Keywords: invA, polymerase chain reaction, Salmonella Gallinarum, Salmonella plasmid virulence C, virulence genes.