Phenotypic and genotypic characterization of locally isolated Salmonella strains used in preparation of Salmonella antigens in Egypt

Research (Published online: 17-12-2016)

16. Phenotypic and genotypic characterization of locally isolated Salmonella strains used in preparation of Salmonella antigens in Egypt -Hazem Mohammed Ibrahim, Dalia Mohammed Adb-Elmoaty, Hanan Ali Ahmed and Mona Ibrahim El-Enbaawy

Veterinary World, 9(12): 1435-1439

   doi: 10.14202/vetworld.2016.1435-1439

Abstract

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Aim: This work was conducted to study the phenotypic and genotypic characterization of locally isolated Salmonella strains (SalmonellaPullorum, Salmonella Enteritidis, and Salmonella Typhimurium) from poultry used in the preparation of Salmonella antigens in Egypt.

Materials and Methods: The phenotypic characterization of Salmonella strains was done using standard microbiological, biochemical, and serological techniques. Molecular identification was done using different sets of primers on different genes using different polymerase chain reaction (PCR) techniques.

Results: The phenotypic characterization of Salmonella strains was confirmed. Molecular identification revealed detection of 284 bp fragment of InvA gene in all studied Salmonella strains. Furthermore, multiplex PCR was used for more confirmation of being Salmonella spp., generally at 429 bp as well as genotyping of Salmonella Typhimurium and Salmonella Enteritidis at 559 and 312 bp, respectively, in one reaction.

Conclusion: The locally isolated field Salmonella strains were confirmed phenotypically and genotypically to be Salmonella Enteritidis, and Salmonella Typhimurium and could be used for the preparation of Salmonella antigens.

Keywords: characterization, duplex polymerase chain reaction, multiplex polymerase chain reaction, Salmonella spp.

Intravenous administration of puppy deciduous teeth stem cells in degenerative valve disease

Research (Published online: 16-12-2016)

15. Intravenous administration of puppy deciduous teeth stem cells in degenerative valve disease - Soontaree Petchdee and Sarunya Sompeewong

Veterinary World, 9(12): 1429-1434

   doi: 10.14202/vetworld.2016.1429-1434

Abstract

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Aim: The objective of this study is to investigate the improvement of heart function in dogs with chronic valvular heart disease after puppy deciduous teeth stem cells (pDSCs) administration.

Materials and Methods: 20 client-owned dogs with degenerative valvular heart disease underwent multiple intravenous injections of allogeneic pDSCs. Dogs were randomly assigned to two groups: (i) Control group (n=10) with standard treatment for heart failure and (ii) group with standard treatment and multiple administrations of pDSCs (n=10). Electrocardiography, complete transthoracic echocardiography, thoracic radiography, and blood pressure were recorded before and after pDSCs injections for 15, 30 and 60 days.

Results: Post pDSCs injection showed measurable improvement in left ventricular ejection fraction, American College of Veterinary Internal Medicine (ACVIM) functional class significantly improved and improved quality of life scores were observed. In the control group, there were no significant enhancements in heart function or ACVIM class.

Conclusions: This finding suggests that pDSCs could be a supplement for valvular heart disease treatment.

Keywords: dental pulp stem cells, dogs, heart failure.

Molecular characterization of field infectious bursal disease virus isolates from Nigeria

Research (Published online: 15-12-2016)

14. Molecular characterization of field infectious bursal disease virus isolates from Nigeria - Ijeoma O. Nwagbo, Ismaila Shittu, Chika I. Nwosuh, George O. Ezeifeka, Frederick J. C. Odibo, Linda O. Michel and Daral J. Jackwood

Veterinary World, 9(12): 1420-1428

   doi: 10.14202/vetworld.2016.1420-1428

Abstract

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Aim: To characterize field isolates of infectious bursal disease virus (IBDV) from outbreaks in nine states in Nigeria through reverse transcription polymerase chain reaction (RT-PCR) and sequence analysis of portions of the VP2 and VP1 genes and to determine the presence or absence of reassortant viruses.

Materials and Methods: A total of 377 bursa samples were collected from 201 suspected IBD outbreaks during 2009 to 2014 from nine states in Nigeria. Samples were subjected to RT-PCR using VP2 and VP1 gene specific primers, and the resulting PCR products were sequenced.

Results: A total of 143 samples were positive for IBDV by RT-PCR. These assays amplified a 743 bp fragment from nt 701 to 1444 in the IBDV VP2 hypervariable region (hvVP2) of segment A and a 722 bp fragment from nt 168 to 889 in the VP1 gene of segment B. RT-PCR products were sequenced, aligned and compared with reference IBDV sequences obtained from GenBank. All but one hvVP2 sequence showed similarity to very virulent IBDV (vvIBDV) reference strains, yet only 3 of the VP1 67 VP1 sequences showed similarity to the VP1 gene of vvIBDV. Phylogenetic analysis revealed a new lineage of Nigerian reassortant IBDV strains.

Conclusion: Phylogenetic analysis of partial sequences of genome segment A and B of IBDV in Nigeria confirmed the existence of vvIBDV in Nigeria. In addition, we noted the existence of reassortant IBDV strains with novel triplet amino acid motifs at positions 145, 146 and 147 in the reassorted Nigerian IBDV.

Keywords: infectious bursal disease virus, Nigeria, novel, reassortant, very virulent.

Evidence of hemolysis in pigs infected with highly virulent African swine fever virus

Research (Published online: 14-12-2016)

13. Evidence of hemolysis in pigs infected with highly virulent African swine fever virus - Zaven Karalyan, Hovakim Zakaryan, Elina Arakelova, Violeta Aivazyan, Marina Tatoyan, Armen Kotsinyan, Roza Izmailyan and Elena Karalova

Veterinary World, 9(12): 1413-1419

   doi: 10.14202/vetworld.2016.1413-1419

Abstract

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Aim: The research was conducted to understand more profoundly the pathogenetic aspects of the acute form of the African swine fever (ASF).

Materials and Methods: A total of 10 pigs were inoculated with ASF virus (ASFV) (genotype II) in the study of the red blood cells (RBCs), blood and urine biochemistry in the dynamics of disease.

Results: The major hematological differences observed in ASFV infected pigs were that the mean corpuscular volume, mean corpuscular hemoglobin, and hematocrits were significantly decreased compared to controls, and the levels of erythropoietin were significantly increased. Also were detected the trends of decrease in RBC count at terminal stages of ASF. Analysis of blood biochemistry revealed that during ASF development, besides bilirubinemia significantly elevated levels of lactate dehydrogenase, and aspartate aminotransferase were detected. Analysis of urine biochemistry revealed the presence of bilirubinuria, proteinuria during ASF development. Proteinuria, especially at late stages of the disease reflects a severe kidney damage possible glomerulonefritis.

Conclusion: The results of this study indicate the characteristics of developing hemolytic anemia observed in acute ASF (genotype II).

Keywords: African swine fever virus, bilirubinemia, bilirubinuria, erythropoietin, hemolysis, proteinuria.

Immunotoxic effect of thiamethoxam in immunized mice with Brucella abortus cultural filtrate antigen

Research (Published online: 13-12-2016)

12. Immunotoxic effect of thiamethoxam in immunized mice with Brucella abortus cultural filtrate antigen - L. H. Salema, M. J. Alwan and Afaf Abdulrahman Yousif

Veterinary World, 9(12): 1407-1412

   doi: 10.14202/vetworld.2016.1407-1412

Abstract

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Aim: This study was planned for determination the toxic effect of thiamethoxam (TMX) in immunized mice with Brucella abortus culture filtrate antigen (CFBAgs) (as a vaccine) and its role of TMX on decrease activity of B. abortus antigen on eliciting of humoral and cellular immunity.

Materials and Methods: To achieve these goals 60 female mice were used, 7-8 weeks age, they were divided equally into three groups (20 in each group) and treated as follows: 1st group: Mice were immunized with CFBAgs intraperitoneally in two doses, 2 weeks intervals with (protein concentration 2 mg\ml), 2nd group: Mice immunized as in the 1st group and was administrated orally with 1/10 lethal dose 50% of TMX (83.7 mg/kg B.W.) for 4 weeks daily, 3rd group was administrated orally with 0.3 ml normal saline served as a control group. At day 28 post immunization (PI) delayed type hypersensitivity (skin test) was done, and serum samples were collected at day 30 (PI) for detection of passive hemagglutination test (PHA); interferon gamma (IFN-γ) which was done by enzyme-linked immunosorbent assay test in addition to phagocytes assay.

Results: The results of skin test post injection with soluble antigen of B. abortus intradermally showed a high significantly mean values at p≤0.05 of footpad skin thickness in the 1st group of mice which recorded (0.51±0.002 mm) as compared with the 2nd group of mice which showed (0.08±0.002 mm) after 24 h; the mean values of skin thickness were declined in the 1st mice (0.46±0.002) and 2nd mice (0.070±0.001) at 48 h; control group showed a negative results. These results were agreed with results of serum levels of IFN-γ (pg/ml) that showed that a significant increase the vaccinated 1st group (406.36±1.52), than those values in the 2nd group (151.61±0.89) and negative result in 3rd group (46.47±0.60), in addition to results of PHA test which showed a significant increase in antibody titer in the 1st group (139±12.16) with low level of serum antibody in the 2nd group (7.66±0.33). Phagocytic ratio results in the 1st group showed an increase to reach (18.55±0.44) than a ratio in the 2nd group (13.24±0.32) and the control group (5.46±0.25).

Conclusion: It was concluded that TMX induced suppression of humoral and cellular immune responses in immunized mice with CFBAgs.

Keywords: Brucella abortus, enzyme-linked immunosorbent assay test, interferon gamma, phagocyte assay, skin test, thiamethoxam.

Effect of feeding Acacia nilotica pod meal on hematobiochemical profile and fecal egg count in goats

Research (Published online: 11-12-2016)

11. Effect of feeding Acacia nilotica pod meal on hematobiochemical profile and fecal egg count in goats - Jitendra Kumar Paswan, Kaushalendra Kumar, Sanjay Kumar, Chandramoni, Abhishek Kumar, Deepak Kumar and Ajit Kumar

Veterinary World, 9(12): 1400-1406

   doi: 10.14202/vetworld.2016.1400-1406

Abstract

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Aim: This study was conducted to observe the effect of feeding Acacia nilotica pod meal on hematobiochemical profile and gastrointestinal parasitic load in growing goats.

Materials and Methods: To experiment was conducted for a period of 3-month on 24 male goats (3½ month old, average body weight [BW] 6.50±1.50 kg), distributed into four groups of six animals each. The experimental animals were fed graded level of A. nilotica pod meal (0%, 10%, 20% and 30%) mixed in concentrate mixture equivalent to tannin concentration of 0%, 1.91%, 3.82% and 5.73% in the total mixed ration I, II, III and IV, respectively, but ad libitum measured quantity of green sorghum fodder (Sorghum bicolor) feeding. The blood samples were collected from experimental goats during the feeding experiment for the examination of different hematological indices and serum biochemical profile to know the overall health status of animals and standard method was followed to analyze the samples. Fecal sample was collected directly from the anus of goats by inserting middle finger and kept the samples in labeled polythene bag. Further fresh sample was processed and examined by McMaster Technique for eggs per gram and oocysts per gram. It gives accurate information regarding severity of infection.

Results: The feeding of babul pod meal did not address significant changes about the hematological parameters among various treatment groups. The lymphocyte count was significantly higher (p=0.07) in T3 group as compared to control and increase with increase in level of babul pod meal in the diet. Blood urea nitrogen (BUN) level was 4.86 and 6.59% lower in T1 and T2 group as compared to control and inversely proportional with level of supplement in ration. The decrease in BUN reflected good dietary protein metabolism happened in animals supplemented with babul pod meal. Serum creatinine level was significantly lower (p<0.01) in T2 group as compared to control. The creatinine level was 20.17% lower in T2 group as compared to control. Haemonchus and Coccidian egg count was significantly reduced (p<0.01) in T2 and T3 group followed with T1 as compared to control group.

Conclusion: The metabolic status of the animal was not affected with the supplementation of babul pod meal, however, lower serum creatinine level and remarkable reduction in nematode, as well as protozoan egg count in the treatment group, showed good health impact of babul pod.

Keywords: Acacia nilotica, goats, hematology, Haemonchus, serum.

Antimicrobial residues in tissues and eggs of laying hens at Chittagong, Bangladesh

Research (Published online: 10-12-2016)

11. Antimicrobial residues in tissues and eggs of laying hens at Chittagong, Bangladesh - Ariful Islam, A. K. M. Saifuddin, Abdullah Al Faruq, Saiful Islam, Shahanaj Shano, Mahabub Alam and Mohammad Mahmudul Hassan

International Journal of One Health, 2: 75-80

  doi: 10.14202/IJOH.2016.75-80

Abstract

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Aim: Antimicrobial residue in animal food products is an important index of food safety. Antimicrobial residues could result from chemotherapeutic or chemoprophylactic use of drugs in food animals. This occurrence of residue in animal food products has received enormous worldwide attention from some local, international, and public health agencies. A crosssectional study was conducted from July to December 2009 to detect the antibiotic residues in tissues and eggs of laying hens at Chittagong of Bangladesh.

Materials and Methods: Microbial inhibition test (MIT) and thin layer chromatography (TLC) methods were used to detect antibacterial residues in poultry tissues (liver, kidney, breast, and thigh muscles) and eggs. The bacteria and pH of the MIT method were as follows: Bacillus subtilis on test agar medium with a pH of 7.2, Bacillus cereus with a pH of 6.0, and Escherichia coli at pH with an 8.0.

Results: The overall prevalence of antibiotic residues detected by MIT was 64% in liver, 63% in kidney, 56% in breast muscle, 50% in thigh muscle, and 60% in eggs. There was significant variation in results between MIT and TLC (p<0.05). Tetracycline residues were found in 48% in liver, 24% in kidneys, 20% in thigh muscles, 26% in breast muscles, and 36% in eggs. Ciprofloxacin residues were found 46% in liver, 42% in kidneys, 34% in thigh muscles, 30% in breast muscles, and 30% in eggs. Enrofloxacin residues were found 40% in livers, 36% in kidneys, 24% in thigh muscles, 20% in breast muscles, and 26% in eggs. Amoxicillin residues were found 48% in livers, 30% in kidneys, 26% in thigh muscles, 22% in breast muscles, and 24% in eggs. The most frequently detected antibiotic residues by both MIT and TLC were found in liver tissue, tetracycline (48%), ciprofloxacin (46%), enrofloxacin (40%), and amoxicillin (42%) were found in liver. Breast muscle tissue was least likely to contain antibiotic residues (24%). Tetracycline (p=0.01) and amoxicillin (p=0.03) residues had significant variation among the various poultry tissues and eggs.

Conclusions: A high percentage of tissues and eggs that could be available for human consumption had antibiotic residues. This study suggests that poultry meat and eggs should not be circulated to markets until the end of the drug’s withdrawal period. It is also recommended to observe the withdrawal period of drugs before poultry slaughter or table egg distribution to avoid antimicrobial resistance and to inform both owners and consumers about the risks of antibiotic residues.

Keywords: antimicrobial, eggs, microbial inhibition test, poultry, thin layer chromatography, tissues.