Prophylactic effect of Nigella sativa against lead acetate induced changes in spermiogram, reproductive hormones and gonadal histology of rats

Research (Published online: 27-11-2016)

23. Prophylactic effect of Nigella sativa against lead acetate induced changes in spermiogram, reproductive hormones and gonadal histology of rats - Mohammed Abdulrazzaq Assi, Mohammed Noor Mohd Hezmee, Yusuf Abba, Md Sabri Md Yusof, Abd Wahid Haron, Mohamed Ali Rajionand Mashaan Abbas Al-Zuhairy

Veterinary World, 9(11): 1305-1311

   doi: 10.14202/vetworld.2016.1305-1311

Abstract

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Aim: This study was designed to evaluate the prophylactic effect of Nigella sativa (NS) treatment on toxic effects induced by lead acetate (LA) on the reproductive hormones, spermiogram and gonadal histology of rats.

Materials and Methods: A total of 20 Sprague-Dawley rats were divided into four groups of five rats each. Group 1 (negative control [NC]) was the NC and was given distilled water, Group 2 served as the positive control (PC) and was administered 10 mg/kg/day of LA per overall survival (OS), Group 3 (T1) was administered 200 mg/kg/daily of NS per OS for a month, and Group 4 (T2) was pretreated with 200 mg/kg/daily of NS per OS for 1 month, followed by 10 mg/kg/daily of LA alone per OS for another. The rats were euthanized at the end of the experimental period for collection of blood and the right caudal epididymis and testis. Serum was used for determination of reproductive hormones by using radioimmunoassay kits. The epididymal segment was cut and homogenized in phosphate-buffered saline, and the homogenate was used for determination of the spermiogram parameters such as sperm concentration, sperm viability, percentage of live sperm, motility and abnormality. Both the epididymis and testis were fixed in 10% buffered formalin for histological processing.

Results: The sperm concentration, general, and individual motilities were higher (p<0.05) in the NC and T1 animals, while the T2 had intermediate and the PC had lower (p<0.05) values of each parameter. The percentage sperm viability was higher (p<0.05) in the T1 and lower (p<0.05) in the PC group. However, percentage abnormality was lower in T1, comparable in NC and T2, and higher (p<0.05) in PC. Spermatogenic cell population and epididymal sperm reserve (ESR) were optimal in control and pretreated animals, while the PC had lower spermatids and ESR. The concentration of estradiol (EST) was lower (p<0.05) in the PC and T2, while leuteinizing hormone (LH) concentration was lower (p<0.05) in the PC, and comparable (p>0.05) between control and T2. The concentration of follicle-stimulating hormone (FSH) was comparable (p>0.05) in all groups, while testosterone (TS) hormone concentration was lower (p<0.05) in the PC and higher in the control and T1 groups.

Conclusion: This study showed the preventive effects of NS administration against alterations in reproductive hormnes, sperm parameters and gonadal histology caused by LA in rats.

Keywords: histology, lead acetate, infertility, Nigella sativa, spermiogram, toxicity.

Seroprevalence of Rotavirus infection in pig population of Arunachal Pradesh

Research (Published online: 27-11-2016)

22. Seroprevalence of Rotavirus infection in pig population of Arunachal Pradesh - G. B. Garam, D. P. Bora, B. Borah, M. Bora and S. K. Das

Veterinary World, 9(11): 1300-1304

   doi: 10.14202/vetworld.2016.1300-1304

Abstract

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Aim: This study was conducted to find out the seroprevalence of Rotavirus(RV) infection among the pig population of Arunachal Pradesh.

Materials and Methods: Serums samples were collected from piglets of age ranging from 1 week to 6 months and the sows associated with the piglets that were reared under organized and unorganized system of management in six different districts of Arunachal Pradesh. The prevalence of RV specific antibodies was detected using a polyclonal antibody-based indirect enzyme-linked immunosorbent assay (i-ELISA).

Results: The study revealed that out of 394 serum samples, 255 (64.72%) samples were found to be positive for RV-specific antibody in i-ELISA. Considering the samples from different districts, Papumpare district of Arunachal Pradesh showed highest numbers of seropositive animals (68.75%) followed by upper Subansiri (64.91%) while West Siang district showed lowest positivity rate (61.22%).

Conclusion: As considerable seropositivity was recorded among pig population of Arunachal Pradesh in this study, there is urgent need to establish high-impact and cost-effective public health intervention tools, key among them being the introduction of strict hygiene practice and RV vaccination program, to greatly reduce the number of deaths due to diarrheal diseases. To the authors’ knowledge, this is the first report on the prevalence of RV infection from pigs of Arunachal Pradesh.

Keywords: antibody, Arunachal Pradesh, pig population, Rotavirus, seropositive.

Allelic and genotypic frequencies in polymorphic Booroola fecundity gene and their association with multiple birth and postnatal growth in Chhotanagpuri sheep

Research (Published online: 25-11-2016)

21. Allelic and genotypic frequencies in polymorphic Booroola fecundity gene and their association with multiple birth and postnatal growth in Chhotanagpuri sheep - Thanesh Oraon, D. K. Singh, Mayukh Ghosh, S. S. Kullu, Rajesh Kumar and L. B. Singh

Veterinary World, 9(11): 1294-1299

   doi: 10.14202/vetworld.2016.1294-1299

Abstract

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Aim: Chhotanagpuri breed of sheep reared for mutton in Jharkhand, India, having problem of low litter size and body weight. The response of genetic improvement for traits with low heritability through traditional selection method is timeconsuming. Therefore, marker-assisted selection based on a polymorphism study of suitable candidate gene can response quickly. Thus, this study was aimed at identification of different allelic and genotypic frequencies of Booroola fecundity (FecB) gene and its association with multiple birth and postnatal growth in Chhotanagpuri sheep.

Materials and Methods: DNA isolation and gene-specific amplification of FecB gene was performed from blood samples of from 92 Chhotanagpuri lambs maintained under similar feeding and  management conditions. Custom nucleotide sequencing and single-strand conformational polymorphism analysis were performed to identify different genotypes with respect to the target gene. Statistical analysis was performed for determination of allelic and genotypic frequencies of FecB gene polymorphisms and its association with multiple birth and postnatal growth of lambs from birth to 52 weeks age.

Results: “AA” “AB” and “BB” genotypes were found at locus-1 as it is polymorphic for FecB gene while locus-2 was found to be monomorphic for FecBgene. Higher frequency of “A” allele at locus-1 was found in single born lambs, whereas “B” allele was predominant among multiple born lambs. The lambs having “BB” genotype weighed significantly (p≤0.01) heavier than those of “AB” and “AA” genotype at 52 weeks of age.

Conclusion: “BB” genotype has emerged as favored genotype for multiple births and better growth indicator. Therefore, homozygous lambs for “B” allele should be selected and utilized in breeding program for better growth rate.

Keywords: body growth, Booroola fecundity gene, Chhotanagpuri sheep, multiple births, single-strand conformational polymorphism.

Value added by Spirulina platensis in two different diets on growth performance, gut microbiota, and meat quality of Japanese quails

Research (Published online: 23-11-2016)

20. Value added by Spirulina platensis in two different diets on growth performance, gut microbiota, and meat quality of Japanese quails -Mohamed S. Yusuf, Marwa A. Hassan, Mohamed M. Abdel-Daim, Adel S. El nabtiti, Ali Meawad Ahmed, Sherief A. Moawed, Ahmed Kamel El-Sayed and Hengmi Cui

Veterinary World, 9(11): 1287-1293

   doi: 10.14202/vetworld.2016.1287-1293

Abstract

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Aim: The growth promoting effect of the blue-green filamentous alga Spirulina platensis (SP) was observed on meat type Japanese quail with antibiotic growth promoter alternative and immune enhancing power.

Materials and Methods: This study was conducted on 180 Japanese quail chicks for 4 weeks to find out the effect of diet type (vegetarian protein diet [VPD] and fish meal protein diet [FMPD])- Spirulina dose interaction (1 or 2 g/kg diet) on growth perfor-mance, gut microbiota, and sensory meat quality of growing Japanese quails (1-5 weeks old).

Results: Data revealed improvement (p<0.05) of weight gain, feed conversion ratio and European efficiency index due to 1, 2 g (SP)/kg VPD, and 2 g (SP)/kg FMPD, respectively. There was a significant decrease of ileum mean pH value by 1 g (SP)/kg VPD. Concerning gut microbiota, there was a trend toward an increase in Lactobacilli count in both 1; 2 g (SP)/kg VPD and 2 g (SP)/kg FMPD. It was concluded that 1 or 2 g (SP)/kg vegetarian diet may enhance parameters of performance without obvious effect on both meat quality and gut microbiota. Moreover, 1 and/or 2 g (SP) may not be invited to share fish meal based diet for growing Japanese quails.

Conclusion: Using of SP will support the profitable production of Japanese quails fed vegetable protein diet.

Keywords: isocaloric, isonitrogenous, meat quality, performances, quails, spirulina.

Emergence of extended spectrum beta-lactamases-producing strains belonging to cefotaxime-M-1 class from intensive care units patients and environmental surfaces in Pakistan

Research (Published online: 22-11-2016)

10. Emergence of extended spectrum beta-lactamases-producing strains belonging to cefotaxime-M-1 class from intensive care units patients and environmental surfaces in Pakistan - Aqsa Ashraf Bukhari, Muhammad Imran Arshad, Shahbaz Raza, Mariya Azam, Sajjad-ur-Rahman and Mashkoor Mohsin

International Journal of One Health, 2: 69-74

  doi: 10.14202/IJOH.2016.69-74

Abstract

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Aim: The emergence of multidrug-resistant (MDR) bacteria is the most dangerous threat for the treatment of infectious diseases. The aim of this study was to detect and characterize extended spectrum beta-lactamases (ESBLs) and carbapenemase-producing Klebsiella pneumoniae and Escherichia coli among patients and environment of intensive care units (ICUs) of three tertiary care hospitals in Pakistan.

Materials and Methods: A total of 82 samples from ICU’s patients and inanimate environment (injection trays, wash basins, door handles, hand swabs of professionals, and ICU fridges) were screened for ESBL by culturing on CHROMagar-ESBL. ESBL and carbapenemases production were confirmed by double disc synergy test and modified Hodge’s test, respectively. Polymerase chain reaction was used to detect ESBL encoding genes bla cefotaxime (CTX-M), blaCTX-M-1, blaCTX-M-2, blaCTX-M-9, blaTEM, blaSHV and carbapenemase genes blaKPC, bla New Delhi metallo-beta-lactamase-1, blaOXA-48 and blaVIM.

Results: Overall, ESBL production was found high 30/82 (36.5%) among isolates of which 15.8% K. pneumoniae and 20.7% E. coli were identified. All the K. pneumoniae and majority of E. coli isolates were MDR, i.e., resistance to three or more antimicrobial categories. Molecular characterization showed the blaCTX-M-1 as the predominant genotype found in 17/21 (80%) of the isolates. None of the strains was found positive for carbapenemase-encoding genes.

Conclusion: In conclusion, this study demonstrates the emergence of MDR ESBL producing strains among ICU patients and hospital environment, posing a serious threat for the control of nosocomial infections.

Keywords: antimicrobial resistance, Escherichia coli, extended spectrum beta lactamase, Klebsiella pneumoniae, nosocomial infections.

Chronic exposure to indoxacarb and pulmonary expression of toll-like receptor-9 in mice

Research (Published online: 21-11-2016)

19. Chronic exposure to indoxacarb and pulmonary expression of toll-like receptor-9 in mice - Sandeep Kaur, C. S. Mukhopadhyay and R. S. Sethi

Veterinary World, 9(11): 1282-1286

   doi: 10.14202/vetworld.2016.1282-1286

Abstract

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Aim: Chronic exposure to indoxacarb and pulmonary expression of toll-like receptor 9 (TLR-9) in mice.

Materials and Methods: In this study, healthy male Swiss albino mice (n=30) aging 8-10 weeks were used to evaluate TLR-9 expression in lungs of mice following indoxacarb exposure with and without lipopolysaccharide (LPS). Indoxacarb was administered orally dissolved in groundnut oil at 4 and 2 mg/kg/day for 90 days. On day 91, five animals from each group were challenged with LPS/normal saline solution at 80 μg/animal. The lung tissues were processed for real time and immunohistochemical studies.

Results: LPS resulted increase in fold change m-RNA expression level of TLR-9 as compare to control, while indoxacarb (4 mg/kg) alone and in combination with LPS resulted 16.21-fold change and 29.4-fold change increase in expression of TLR-9 m-RNA, respectively, as compared to control. Similarly, indoxacarb (2 mg/kg) alone or in combination with LPS also altered TLR-9 expression. Further at protein level control group showed minimal expression of TLR-9 in lungs as compare to other groups, however, LPS group showed intense positive staining in bronchial epithelium as well as in alveolar septal cells. Indoxacarb at both doses individually showed strong immuno-positive reaction as compare to control, however when combined with LPS resulted intense staining in airway epithelium as compare to control.

Conclusion: Chronic oral administration of indoxacarb for 90 days (4 and 2 mg/kg) alters expression of TLR-9 at m-RNA and protein level and co-exposure with LPS exhibited synergistic effect.

Keywords: indoxacarb, lipopolysaccharide, lungs, mice, toll-like receptor-9.

Bacterial canine vector-borne zoonotic diseases in “One Health” concept

Review (Published online: 20-11-2016)

9. Bacterial canine vector-borne zoonotic diseases in “One Health” concept - George Valiakos

International Journal of One Health, 2: 58-68

  doi: 10.14202/IJOH.2016.58-68

Abstract

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Canine vector-borne diseases constitute a large group of diseases transmitted by arthropods with worldwide distribution. A wide range of bacterial, viral, and parasitic agents that are transmitted by vectors cause disease to dogs, many of which can also affect humans and thus have an important zoonotic potential. Bacterial agents that are transmitted by vectors have been considered less important than viral or parasitic agents and are not commonly discussed in companion animal practice. However, close contact between pet animals and people offers favorable conditions for transmission of these bacteria. Many of these diseases have become a focus of interest for scientists in recent years. Increase in reservoir abundance, climate change, changing habitat structure, socio-political changes, and imports of dogs for welfare reasons and trade as well as traveling are considered to be potential factors for the pathogens and vectors introduction into new areas. Apart from, the veterinary aspect of these diseases, domestic dogs could play a central epidemiological role in the transmission of bacterial agents to humans, acting as reservoirs and sentinels, a circumstance that requires a One Health approach. This review highlights the most important of these bacterial agents, presenting updated current knowledge with special reference to treatment approach and One Health aspect.

Keywords: bacteria, canine, One Health, vectors, zoonoses.