The effect of lead on the process of spermatogenesis in sex glands of male albino rats

Research (Published online: 23-10-2016)

15. The effect of lead on the process of spermatogenesis in sex glands of male albino rats - Olga Sergeevna Shubina and Natalia Anatolievna Dudenkova

Veterinary World, 9(10): 1129-1134

   doi: 10.14202/vetworld.2016.1129-1134

Abstract

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Aim: The aim of this study was to investigate the morphological and functional features of the process of spermatogenesis in the testes of male albino rats when exposed to lead acetate.

Materials and Methods: Using histological, morphometric and statistical methods explored the effects of lead acetate on the process of spermatogenesis in the testes of male albino rats. Investigations were carried out using a digital microscope Axio Imager.M2 and automatic cell counter Countess™.

Results: It is shown that when exposed to lead acetate a decrease in the production of all populations of spermatogenic cells, decreased spermatogenesis index and an index of relaxation (tension spermatogenesis), the increase in the index of ripening, index meiotic activity and germinative index, which indicates a decrease in the functional activity of the testes. On preparations, it is possible to see that after the influence of acetate of lead the head of spermatozoa becomes more roundish, breaks of tails observed.

Conclusion: Lead acetate reduces the productivity of the seminal glands, which leads to the decrease of the concentration of spermatozoa, and their viability. The results of the studies suggest a negative impact of lead acetate in the course of the process of spermatogenesis in the testes of male white rats.

Keywords: epididymis (appendage testis), lead acetate, seminal glands (testis), Sertoli cells, spermatogenesis, spermatogenic cells.

Detection of Salmonella species in chicken carcasses using genus specific primer belong to invA gene in Sohag city, Egypt

Research (Published online: 22-10-2016)

14. Detection of Salmonella species in chicken carcasses using genus specific primer belong to invA gene in Sohag city, Egypt - Nahed Mahmoud Abdel-Aziz

Veterinary World, 9(10): 1125-1128

   doi: 10.14202/vetworld.2016.1125-1128

Abstract

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Aim: This study aimed to detect Salmonella species found as contaminants in chicken carcass (thigh, breast, wings, liver, and gizzard).

Materials and Methods: A total of 75 chicken samples including thigh, breast, wings, liver, and gizzard (15 of each) were collected from different markets in Sohag city for detection of Salmonella species by culture methods, biochemical tests, serology, and polymerase chain reaction.

Results: The overall incidence of Salmonella contamination of 75 examined samples was found to be 6.6% with the higher percentage of Salmonella being isolated from liver samples (13.3%) followed by thigh, wings, gizzard (6.6%) while breast show negative result.

Conclusion: Results in this study indicate that contamination of chicken carcass with Salmonella needs strict hygienic measures to prevent their transmission to human.

Keywords: chicken carcass, invA gene, Salmonella spp.

Effect of Tamarindus indica leaf powder on plasma concentrations of copper, zinc, and iron in fluorotic cows

Research (Published online: 21-10-2016)

13. Effect of Tamarindus indica leaf powder on plasma concentrations of copper, zinc, and iron in fluorotic cows - Pinaki Samal, R. C. Patra, A. R. Gupta, S. K. Mishra, D. Jena and D. Satapathy

Veterinary World, 9(10): 1121-1124

   doi: 10.14202/vetworld.2016.1121-1124

Abstract

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Aim: The main objective of the study was to determine the deleterious effect of fluoride on plasma trace minerals of fluorotic cattle and to evaluate the effect of Tamarindus indica leaf powder toward correction of the same.

Materials and Methods: A total of 30 cattle exhibiting chronic sign of fluorosis and 10 healthy cattle from nonfluorotic area were incorporated in this study. Fluorotic cattle were divided into three equal groups consisting of 10 cattle each. Group I from fluoride free area served as healthy control. The Group II received no treatment and served as disease control. Groups III and IV were supplemented with tamarind leaf powder at 15 g and 30 g/day with feed for 60 days. Plasma mineral status was evaluated after 60 days of treatment with double beam atomic absorption spectrophotometer.

Results: Statistical analysis of data revealed a significant (p<0.05) decrease in mean plasma copper (Cu) (0.344±0.007 ppm), zinc (Zn) (0.692±0.06 ppm), and iron (Fe) concentration (1.100±0.01 ppm) in fluorotic cattle in comparison to healthy cattle (0.58±0.010, 2.342±0.04, 1.406±0.04 ppm, respectively). Significant (p<0.05) increase in Cu, Zn, and Fe was recorded after supplementation of tamarind leaf powder to the fluorotic cattle.

Conclusion: It was concluded that fluorotic cattle might be supplemented with T. indica leaf powder with feed for the correction of the decreased level of certain plasma minerals.

Keywords: cattle, fluorosis, micro-minerals, tamarind leaf.

Comparison of radiography and ultrasonography in the detection of lung and liver cysts in cattle and buffaloes

Research (Published online: 20-10-2016)

12. Comparison of radiography and ultrasonography in the detection of lung and liver cysts in cattle and buffaloes - Ashwani Kumar, Narinder Singh Saini, Jitender Mohindroo, Balbir Bagicha Singh, Vandana Sangwan and Naresh Kumar Sood

Veterinary World, 9(10): 1113-1120

   doi: 10.14202/vetworld.2016.1113-1120

Abstract

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Aim: Echinococcosis is the major cause of lung and liver cysts in ruminants. This study compared usefulness of radiography and ultrasonography (USG) in the detection of lung and/or liver cysts in sick bovine animals. The study also worked out cooccurrence of lung and liver cysts, and whether these cysts were primary cause of sickness or not.

Materials and Methods: This study was conducted on 45 sick bovine (37 buffaloes and 8 cattle) suffering from lung and liver cysts. A complete history of illness and clinical examination was carried out. Lateral radiographs of chest and reticular region were taken. In radiographically positive or suspected cases of cysts, USG of the lung and liver region was done. Depending on the location of cyst and clinical manifestations of the animal, the cysts were categorized as primary or secondary causes of sickness.

Results: Using either imaging technique, it was observed that 46.7% of the animals had both lung and liver cysts, whereas 33.3% had only lung and 20% had only liver cyst. Cysts were identified as primary cause of sickness in 31.1% animals only. For diagnosing lung cysts, radiography (71.1%) and USG (62.2%) had similar diagnostic utility. However, for detecting liver cysts, USG was the only imaging tool.

Conclusion: The lung and liver cysts, depending on their number and size may be a primary cause of sickness in bovine. Radiography and USG are recommended, in combination, as screening tools to rule out echinococcosis.

Keywords: bovine, echinococcus cyst, liver, lung, radiography, ultrasound.

Production performance of finisher broiler fed with cocoyam-corm meal as partial energy replacement for maize

Research (Published online: 19-10-2016)

11. Production performance of finisher broiler fed with cocoyam-corm meal as partial energy replacement for maize - Christian Paul P. de la Cruz

Veterinary World, 9(10): 1107-1112

   doi: 10.14202/vetworld.2016.1107-1112

Abstract

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Aim: The objective of this study was to evaluate the potential of Gabing San Fernando (Xanthosoma spp.) corms as partial carbohydrate replacement for maize in finisher broiler production.

Materials and Methods: The completely randomized design was utilized to investigate the effects of three finisher poultry diets prepared in varying amounts of cocoyam-corm meal set at 0% (control), 25%, and 50% (experimental) replacement levels.

Results: There were no significant differences (p≥0.05) as to mortality and body weight measurements between control and experimental groups. Similarly, the mean weights of selected internal organs and condemnable carcasses among treatment groups did not show any significant differences (p≥0.05). In terms of the average feed intakes, birds from 50%-cocoyam group had the highest mean value and were found to be statistically different (p≥0.01) from both control and 25%-cocoyam groups. However, feed conversion ratio did not significantly differ (p≥0.05) among three groups. Higher feed costs were associated with the 50%-cocoyam treatment diet, which was only consistent with higher feed inputs. Thus, the group fed with 50%-cocoyam meal had significantly higher total mean production costs (p<0.005) per bird, when other expenses were taken into account. The production costs for the group given 25%-cocoyam meal did not significantly differ (p≥0.05) from the control group.

Conclusion: Partial replacement of maize with cocoyam-corm meal at 25% level was acceptable since inclusion at this level did not adversely affect the production performance of finisher broilers in terms of growth rate, mortality rate, and feeding efficiency. The use of cocoyam meal as nonconventional and alternative carbohydrate source in poultry diet presents positive economic implications, especially to smallhold farmers from the developing countries, like the Philippines.

Keywords: broiler, cocoyam, feed-conversion ratio, Philippines, poultry, Xanthosoma.

Certain hormonal profiles of postpartum anestrus jersey crossbred cows treated with controlled internal drug release and ovsynch protocol

Research (Published online: 18-10-2016)

10. Certain hormonal profiles of postpartum anestrus jersey crossbred cows treated with controlled internal drug release and ovsynch protocol - Dayanidhi Jena, S. Das, B. K. Patra, S. S. Biswal, D. N. Mohanty and P. Samal

Veterinary World, 9(10): 1102-1106

   doi: 10.14202/vetworld.2016.1102-1106

Abstract

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Aim: The study was conducted to determine the serum levels of certain hormones in post-partum anestrus cows following treatment with controlled internal drug release (CIDR) and Ovsynch protocol.

Materials and Methods: A total of 30 postpartum anestrus cows were divided into three equal groups after thorough gynecoclinical examination. The Group 1 animals received an intravaginal progesterone device on day 0 and 2 ml of prostaglandin F_2α (PGF_2α) on day of CIDR removal (7th day), Group 2 cows were treated with ovsynch protocol (gonadotropinreleasing hormone [GnRH]-PGF_2α-GnRH) on day 0, 7 and 9, respectively, and Group 3 cows were supplemented with mineral mixture and treated as control. The serum estrogen, progesterone, triiodothyronine, and thyroxine concentration were estimated using enzyme-linked immunosorbent assay kit and absorbance was read at 450 nm with Perkin Elmer Wallac 1420 Microplate Reader.

Results: There was a significant increase in progesterone level in Group 1 after withdrawal of CIDR as compared to other two groups. However, the estrogen assay revealed a greater concentration in Group 2 against Group 1 on day 7 of sampling. However, there was no significant difference for serum triiodothyronine (T₃) and thyroxine (T₄) irrespective of treatment protocols and days of sampling.

Conclusion: Treatment with CIDR based progesterone therapy and drug combinations may affect the reproductive hormonal balance like estrogen and progesterone, which is inevitable for successful return to cyclicity and subsequent fertilization and conception. However, as far as serum T₃ and T₄ concentration concerned it may not give an astounding result.

Keywords: controlled internal drug release, ovsynch, postpartum anestrus.

Morphological and molecular identification of the brown dog tick Rhipicephalus sanguineus and the camel tick Hyalomma dromedarii(Acari: Ixodidae) vectors of Rickettsioses in Egypt

Research (Published online: 18-10-2016)

9. Morphological and molecular identification of the brown dog tick Rhipicephalus sanguineus and the camel tick Hyalomma dromedarii(Acari: Ixodidae) vectors of Rickettsioses in Egypt - Hend H. A. M. Abdullah, Amal El-Molla, Fayez A. Salib, Nesreen A. T. Allam, Alaa A. Ghazy and Sobhy Abdel-Shafy

Veterinary World, 9(10): 1087-1101

   doi: 10.14202/vetworld.2016.1087-1101

Abstract

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Aim: Rickettsioses have an epidemiological importance that includes pathogens, vectors, and hosts. The dog tick Rhipicephalus sanguineus and the camel tick Hyalomma dromedarii play important roles as vectors and reservoirs of Rickettsiae. The aim of this study was to determine the prevalence of Rickettsiae in ixodid ticks species infesting dogs and camels in Egypt, in addition to, the morphological and molecular identification of R. sanguineus and H. dromedarii.

Materials and Methods: A total of 601 and 104 of ticks’ specimens were collected from dogs and camels, respectively, in Cairo, Giza and Sinai provinces. Hemolymph staining technique and OmpA and gltA genes amplification were performed to estimate the prevalence rate of Rickettsiae in ticks. For morphological identification of tick species, light microscope (LM) and scanning electron microscope (SEM) were used. In addition to the phylogenetic analyses of 18S rDNA, Second internal transcript spacer, 12S rDNA, cytochrome c oxidase subunit-1, and 16S rDNA were performed for molecular identification of two tick species.

Results: The prevalence rate of Rickettsiae in ticks was 11.6% using hemolymph staining technique and 6.17% by OmpA and gltA genes amplification. Morphological identification revealed that 100% of dogs were infested by R. sanguineus while 91.9% of camels had been infested by H. dromedarii. The phylogenetic analyses of five DNA markers confirmed morphological identification by LM and SEM. The two tick species sequences analyses proved 96-100% sequences identities when compared with the reference data in Genbank records.

Conclusion: The present studies confirm the suitability of mitochondrial DNA markers for reliable identification of ticks at both intra- and inter-species level over the nuclear ones. In addition to, the detection of Rickettsiae in both ticks’ species and establishment of the phylogenetic status of R. sanguineus and H. dromedarii would be useful in understanding the epidemiology of ticks and tick borne rickettsioses in Egypt.

Keywords: hard ticks, light microscope, phylogenetic analysis, polymerase chain reaction, Rickettsiae, scanning electron microscope.