Polyadenylated tail length variation pattern in ultra-rapid vitrified bovine oocytes

Research (Published online: 14-10-2016)

6. Polyadenylated tail length variation pattern in ultra-rapid vitrified bovine oocytes - D. J. Dutta, Himangshu Raj and Hiramoni Dev

Veterinary World, 9(10): 1070-1074

   doi: 10.14202/vetworld.2016.1070-1074

Abstract

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Aim: The current study aims at investigating the polyadenylated (poly[A]) tail length of morphologically high and low competent oocytes at different developmental stages. Furthermore, effect of ultra-rapid vitrification on the poly(A) tail length was studied.

Materials and Methods: Fresh bovine cumulus oocyte complexes from abattoir originated ovaries were graded based on morphological characters and matured in vitro. Cryopreservation was done by ultra-rapid vitrification method. mRNA was isolated from different categories of oocyte and subjected to ligation-mediated poly(A) test followed by polymerase chain reaction for determining the poly(A) tail length of β actin, gap junction protein alpha 1 (GJA1), poly(A) polymerase alpha (PAPOLA), and heat shock 70 kDa protein (HSP70) transcripts.

Results: GJA1, PAPOLA, and HSP70 showed significantly higher poly(A) in immature oocytes of higher competence irrespective of vitrification effects as compared to mature oocytes of higher competence.

Conclusion: mRNA poly(A) tail size increases in developmentally high competent immature bovine oocytes. There was limited effect of ultra-rapid vitrification of bovine oocytes on poly(A).

Keywords: ligation-mediated poly(A) test, oocyte, poly-adenylated tail, vitrification.

Molecular identification of Mycoplasma synoviae from seroprevalent commercial breeder farms at Chittagong district, Bangladesh

Research (Published online: 10-10-2016)

5. Molecular identification of Mycoplasma synoviae from seroprevalent commercial breeder farms at Chittagong district, Bangladesh - Md. Inkeyas Uddin, Md. Harisul Abid, Md. Shafiqul Islam, Tofazzal Md. Rakib, Ashim Baran Sen, Shah Mohammed Ziqrul Haq Chowdhury, Md. Nurul Anwar and Kazi Md. Kamaruddin

Veterinary World, 9(10): 1063-1069

   doi: 10.14202/vetworld.2016.1063-1069

Abstract

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Aim: Worldwide, Mycoplasma synoviae (MS) is an important pathogen of poultry, especially for chicken and turkey. It causes respiratory tract infection and infectious sinusitis. The study was conducted to determine the seroprevalence of MS infection with associated risk factors and identification of MS organism in unvaccinated flocks of commercial breeder farms of the Chittagong district, Bangladesh.

Materials and Methods: A total of 365 serum samples were collected and tested for MS using serum plate agglutination (SPA) test for determination of MS seroprevalence. On the other hand, tracheal swabs were collected from each seropositive flocks for polymerase chain reaction (PCR) to determine the presence of MS organism.

Results: Among the farms, the highest prevalence was found to be 69% and the lowest prevalence was 28% with the average 60%. The seroprevalence of MS infection in breeder farms was highest 70% with the flock size >10,000 birds, whereas it was lowest 57% in the flocks ranging from 4000 to 7000. According to age group, the prevalence was found highest 70% in >60 weeks age group of birds and lowest 42% in 10-19 weeks group. The seroprevalence of MS in winter season was found as highest as 64%, whereas it was found lowest 60% in the summer season. There was a statistically significant difference (p<0.01) among the seroprevalence of MS in different breeder farms, flock size, and age groups, but there was no significant (p>0.05) difference in the winter, summer, and rainy season. To confirm the presence of MS in the samples, PCR test was applied using specific primers to amplify a 214 bp region of the 16S rRNA gene of the organism. In PCR, all seropositive flocks showed a positive result for MS.

Conclusion: As the plate agglutination test result showed 100% similar with PCR result, it can be suggested that agglutination test is better than molecular and culture techniques for MS detection and it is also cheaper and less time-consuming method.

Keywords: breeder farm, Mycoplasma synoviae, polymerase chain reaction, respiratory infection, risk factors, seroprevalence.

Determination of ceruloplasmin, some other acute phase proteins, and biochemical parameters in cows with endometritis

Research (Published online: 08-10-2016)

4. Determination of ceruloplasmin, some other acute phase proteins, and biochemical parameters in cows with endometritis - S. Kaya, O. Merhan, C. Kacar, A. Colak and K. Bozukluhan

Veterinary World, 9(10): 1056-1062

   doi: 10.14202/vetworld.2016.1056-1062

Abstract

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Aim: The aim of this study is to determine serum ceruloplasmin levels in cows with endometritis of varying degrees of severity and to establish whether or not there is a correlation between acute phase protein (APP) levels and biochemical parameters.

Material and Methods: The study was conducted with 100 Brown Swiss cows (3-8 years of age) on days 28-32 postpartum. Cows were divided into endometritis (mild, moderate, and severe endometriosis) and healthy groups based on ultrasonography, vaginoscopy, and cytological examination. Blood samples were collected from all cows. Levels of haptoglobin (Hp), serum amyloid A (SAA), ceruloplasmin, albumin, and some biochemical parameters were analyzed.

Results: Hp, SAA, and ceruloplasmin levels were higher in cows with endometritis than in healthy cows (p=0.001), and the levels of these APPs increased as endometritis became more severe (p=0.001). Some significant correlations were found between APPs and the biochemical parameters that were analyzed. In conclusion, it was determined that ceruloplasmin levels increase significantly in the presence of endometritis and proportionate to the severity of endometritis. A significant correlation was found between ceruloplasmin levels and Hp and SAA levels.

Conclusion: It was concluded that ceruloplasmin levels can be used in the diagnosis of endometritis as an alternative to Hp and SAA levels.

Keywords: albumin, ceruloplasmin, cow, endometritis, haptoglobin, serum amyloid A.

Expression of heat-shock protein 72 mRNA in relation to heart rate variability of Sahiwal and Karan-Fries in different temperature-humidity indices

Research (Published online: 07-10-2016)

3. Expression of heat-shock protein 72 mRNA in relation to heart rate variability of Sahiwal and Karan-Fries in different temperature-humidity indices - Prava Mayengbam, T. C. Tolenkhomba and R. C. Upadhyay

Veterinary World, 9(10): 1051-1055

   doi: 10.14202/vetworld.2016.1051-1055

Abstract

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Aim: To investigate the effect of temperature-humidity index (THI) on the expression pattern of heat-shock protein 72 (HSP72) mRNA of Sahiwal and Karan-Fries (KF) cattle in different THIs.

Materials and Methods: Five different periods of a year were selected based on combinations of T_max/T_min, viz., P1: <20°C/<10°C; P2: >20°C/<10°C, P3: <30°C/<15°C; P4: >35°C/<20°C, and P5: >35°C/>20°C. The THI was calculated from the records of temperature and relative humidity in different periods. Heart rate variability (HRV) was calculated from electrocardiogram records in different periods. HSP72 mRNA expression was estimated by reverse transcription polymerase chain reaction.

Results: The THI recorded during P1, P2, P3, P4, and P5 were 55.5, 60.3, 70.1, 74.5, and 79.0, respectively. THI in P4 and P5 were stressful to animals. HSP72 mRNA expression increased during cold stress in P1 in Sahiwal and heat stress in P4 and P5 in both Sahiwal and KF. Sahiwal maintained increased HSP72 mRNA expression longer than KF without causing a significant change in HRV.

Conclusion: Both low THI in winter and high THI in summer increased HSP72 mRNA of Sahiwal and KF without significant change in HRV. Thermotolerance of Sahiwal could be due to the maintenance of higher HSP72 expression longer than KF in prolonged heat stress in summer.

Keywords: heat-shock protein 72 mRNA, Karan-Fries, Karnal, Sahiwal, stress, temperature-humidity index.

Zoonotic intestinal protozoan of the wild boars, Sus scrofa, in Persian Gulf’s coastal area (Bushehr province), Southwestern Iran

Research (Published online: 06-10-2016)

2. Zoonotic intestinal protozoan of the wild boars, Sus scrofa, in Persian Gulf’s coastal area (Bushehr province), Southwestern Iran -Kambiz Yaghoobi, Bahador Sarkari, Majid Mansouri and Mohammad Hossein Motazedian

Veterinary World, 9(10): 1047-1050

   doi: 10.14202/vetworld.2016.1047-1050

Abstract

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Aim: Wild boars, Sus scrofa, are potential reservoirs of many zoonotic diseases, and there are a possibility of transmission of the zoonotic diseases from these animals to humans and also domestic animals. This study aimed to evaluate the protozoan contamination of wild boars in the Persian Gulf’s coastal area (Bushehr Province), southwestern Iran.

Materials and Methods: A total of 25 crossbred boars were collected during a course of vertebrate pest control in Bushehr province, in 2013. Samples were collected from the gastrointestinal tracts of each boar in 5% formalin, Bouin’s solution, sodium acetate-acetic acid-formalin, and polyvinyl alcohol fixatives. Fixed stool smears examined by trichrome and Ziehl–Neelsen staining.

Results: Each of the 25 wild boars was infected with at least one of the intestinal protozoans. The rate of contamination with intestinal protozoan was 64% for Balantidium coli, 76% for Iodamoeba sp., 52% for Entamoeba polecki, 44% for Blastocystis sp. and 8% forChilomastix sp. No intestinal coccidian was detected in studied boars when the stool samples were evaluated by Ziehl–Neelsen staining method.

Conclusion: Findings of this study demonstrated that wild boars in the Persian Gulf coastal area are contaminated by many protozoans, including zoonotic protozoan, which poses a potential risk to locals as well as the domestic animals of the area.

Keywords: Iran, Persian Gulf, protozoan, wild boars, zoonosis.

Effect of feeding guar meal on nutrient utilization and growth performance in Mahbubnagar local kids

Research (Published online: 04-10-2016)

1. Effect of feeding guar meal on nutrient utilization and growth performance in Mahbubnagar local kids - Razia Sultana Janampet, Kishan Kumar Malavath, Rajanna Neeradi, Satyanarayana Chedurupalli and Raghunandan Thirunahari

Veterinary World, 9(10): 1043-1046

   doi: 10.14202/vetworld.2016.1043-1046

Abstract

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Aim: This study was conducted to evaluate the growth performance and nutrient digestibility of guar meal, an unconventional

protein-rich feed ingredient in kids in comparison to conventional groundnut cake.

Materials and Methods: A total of 18 kids were randomly allotted to three groups, and T1 group was fed on basal diet, T2 and T3 groups were offered diet replacing groundnut cake at 50% and 100% with guar meal, respectively, for a period of 120-day. At the end of the growth trial, a digestibility trial was conducted to evaluate the nutrient utilization.

Results: There was no significant difference in dry matter intake among three groups. Nutrient digestibilities were significantly higher (p<0.05) in kids fed T2 ration with 50% replacement of groundnut cake with guar meal.

Conclusion: It can be concluded that guar meal can be incorporated at 50% level in the concentrate mixture of goats replacing groundnut cake without any adverse effects.

Keywords: growth performance, guar meal, nutrient utilization.

Prevalence and multidrug resistance pattern of Salmonella isolated from resident wild birds of Bangladesh

Research (Published online: 01-10-2016)

7. Prevalence and multidrug resistance pattern of Salmonella isolated from resident wild birds of Bangladesh - Abdullah Al Faruq, Mohammad Mahmudul Hassan, Mohammad Mejbah Uddin, Mohammad Lutfur Rahman, Tofazzal Md. Rakib, Mahabub Alam and Ariful Islam

International Journal of One Health, 2: 35-41

  doi: 10.14202/IJOH.2016.35-41

Abstract

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Aim: Salmonellosis is one of the most common zoonotic diseases, and the presence of antimicrobial resistant Salmonella in wild birds is global public health threat. Throughout the last decades, multidrug resistance of Salmonella spp. has increased, particularly in developing countries. Therefore, a cross-sectional study was conducted to investigate the prevalence of Salmonella spp. and antimicrobial resistance pattern against Salmonella spp. from two species of resident wild birds namely house crow (Corvus splendens) and Asian pied starling (Gracupica contra).

Materials and Methods: Samples were collected from cloacal swabs of house crows and Asian pied starling for isolating Salmonella spp. (bacteriological culture methods) followed by antimicrobial susceptibility testing (disk diffusion method) against Salmonella spp. isolates during March to December 2014.

Results: The prevalence of Salmonella in Asian pied starling and house crows were 67% and 65%, respectively. Within the category of samples from different species, the variation in prevalence was not varied significantly (p>0.05). Isolated Salmonella spp. was tested for resistance to six different antimicrobial agents. Among six antimicrobial tested, 100% resistance were found to penicillin, oxacillin, and clindamycin followed by erythromycin (50-93%), kanamycin (7-20%), and cephalothin (30-67%) from both species of birds. Kanamycin remained sensitive in (70-73%), cephalothin (26-70%), and erythromycin appeared to be (0-30%) sensitive against Salmonella spp. isolates. Isolated Salmonella spp. was multidrug resistant up to three of the six antimicrobials tested.

Conclusion: It can be said that the rational use of antimicrobials needs to be adopted in the treatment of disease for livestock, poultry, and human of Bangladesh to limit the emergence of drug resistance toSalmonella spp.

Keywords: antimicrobial, prevalence, resistance, resident wild birds, Salmonella.