Zoonotic intestinal protozoan of the wild boars, Sus scrofa, in Persian Gulf’s coastal area (Bushehr province), Southwestern Iran

Research (Published online: 06-10-2016)

2. Zoonotic intestinal protozoan of the wild boars, Sus scrofa, in Persian Gulf’s coastal area (Bushehr province), Southwestern Iran -Kambiz Yaghoobi, Bahador Sarkari, Majid Mansouri and Mohammad Hossein Motazedian

Veterinary World, 9(10): 1047-1050

   doi: 10.14202/vetworld.2016.1047-1050

Abstract

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Aim: Wild boars, Sus scrofa, are potential reservoirs of many zoonotic diseases, and there are a possibility of transmission of the zoonotic diseases from these animals to humans and also domestic animals. This study aimed to evaluate the protozoan contamination of wild boars in the Persian Gulf’s coastal area (Bushehr Province), southwestern Iran.

Materials and Methods: A total of 25 crossbred boars were collected during a course of vertebrate pest control in Bushehr province, in 2013. Samples were collected from the gastrointestinal tracts of each boar in 5% formalin, Bouin’s solution, sodium acetate-acetic acid-formalin, and polyvinyl alcohol fixatives. Fixed stool smears examined by trichrome and Ziehl–Neelsen staining.

Results: Each of the 25 wild boars was infected with at least one of the intestinal protozoans. The rate of contamination with intestinal protozoan was 64% for Balantidium coli, 76% for Iodamoeba sp., 52% for Entamoeba polecki, 44% for Blastocystis sp. and 8% forChilomastix sp. No intestinal coccidian was detected in studied boars when the stool samples were evaluated by Ziehl–Neelsen staining method.

Conclusion: Findings of this study demonstrated that wild boars in the Persian Gulf coastal area are contaminated by many protozoans, including zoonotic protozoan, which poses a potential risk to locals as well as the domestic animals of the area.

Keywords: Iran, Persian Gulf, protozoan, wild boars, zoonosis.

Effect of feeding guar meal on nutrient utilization and growth performance in Mahbubnagar local kids

Research (Published online: 04-10-2016)

1. Effect of feeding guar meal on nutrient utilization and growth performance in Mahbubnagar local kids - Razia Sultana Janampet, Kishan Kumar Malavath, Rajanna Neeradi, Satyanarayana Chedurupalli and Raghunandan Thirunahari

Veterinary World, 9(10): 1043-1046

   doi: 10.14202/vetworld.2016.1043-1046

Abstract

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Aim: This study was conducted to evaluate the growth performance and nutrient digestibility of guar meal, an unconventional

protein-rich feed ingredient in kids in comparison to conventional groundnut cake.

Materials and Methods: A total of 18 kids were randomly allotted to three groups, and T1 group was fed on basal diet, T2 and T3 groups were offered diet replacing groundnut cake at 50% and 100% with guar meal, respectively, for a period of 120-day. At the end of the growth trial, a digestibility trial was conducted to evaluate the nutrient utilization.

Results: There was no significant difference in dry matter intake among three groups. Nutrient digestibilities were significantly higher (p<0.05) in kids fed T2 ration with 50% replacement of groundnut cake with guar meal.

Conclusion: It can be concluded that guar meal can be incorporated at 50% level in the concentrate mixture of goats replacing groundnut cake without any adverse effects.

Keywords: growth performance, guar meal, nutrient utilization.

Prevalence and multidrug resistance pattern of Salmonella isolated from resident wild birds of Bangladesh

Research (Published online: 01-10-2016)

7. Prevalence and multidrug resistance pattern of Salmonella isolated from resident wild birds of Bangladesh - Abdullah Al Faruq, Mohammad Mahmudul Hassan, Mohammad Mejbah Uddin, Mohammad Lutfur Rahman, Tofazzal Md. Rakib, Mahabub Alam and Ariful Islam

International Journal of One Health, 2: 35-41

  doi: 10.14202/IJOH.2016.35-41

Abstract

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Aim: Salmonellosis is one of the most common zoonotic diseases, and the presence of antimicrobial resistant Salmonella in wild birds is global public health threat. Throughout the last decades, multidrug resistance of Salmonella spp. has increased, particularly in developing countries. Therefore, a cross-sectional study was conducted to investigate the prevalence of Salmonella spp. and antimicrobial resistance pattern against Salmonella spp. from two species of resident wild birds namely house crow (Corvus splendens) and Asian pied starling (Gracupica contra).

Materials and Methods: Samples were collected from cloacal swabs of house crows and Asian pied starling for isolating Salmonella spp. (bacteriological culture methods) followed by antimicrobial susceptibility testing (disk diffusion method) against Salmonella spp. isolates during March to December 2014.

Results: The prevalence of Salmonella in Asian pied starling and house crows were 67% and 65%, respectively. Within the category of samples from different species, the variation in prevalence was not varied significantly (p>0.05). Isolated Salmonella spp. was tested for resistance to six different antimicrobial agents. Among six antimicrobial tested, 100% resistance were found to penicillin, oxacillin, and clindamycin followed by erythromycin (50-93%), kanamycin (7-20%), and cephalothin (30-67%) from both species of birds. Kanamycin remained sensitive in (70-73%), cephalothin (26-70%), and erythromycin appeared to be (0-30%) sensitive against Salmonella spp. isolates. Isolated Salmonella spp. was multidrug resistant up to three of the six antimicrobials tested.

Conclusion: It can be said that the rational use of antimicrobials needs to be adopted in the treatment of disease for livestock, poultry, and human of Bangladesh to limit the emergence of drug resistance toSalmonella spp.

Keywords: antimicrobial, prevalence, resistance, resident wild birds, Salmonella.

Comparative evaluation of polymerase chain reaction assay with microscopy for detection of asymptomatic carrier state of theileriosis in a herd of crossbred cattle

Research (Published online: 30-09-2016)

21. Comparative evaluation of polymerase chain reaction assay with microscopy for detection of asymptomatic carrier state of theileriosis in a herd of crossbred cattle - Gaurav Charaya, N. K. Rakha, Sushila Maan, Aman Kumar, Tarun Kumar and Ricky Jhambh

Veterinary World, 9(9): 1039-1042

   doi: 10.14202/vetworld.2016.1039-1042

Abstract

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Aim: This study aims to develop and to standardize a polymerase chain reaction (PCR) assay that will diagnose clinical as well as carrier state of the disease and to compare the results with conventional microscopy technique.

Materials and Methods: A herd of crossbred cattle with the previous history of theileriosis in village Lahli, district Rohtak, Haryana, was selected for this study. A total of 29 blood samples were collected randomly from cows including five clinically ill cattle. Blood smears from all animals and lymph node biopsy smears from animal with swollen lymph nodes were examined microscopically after conventional Giemsa staining. Phenol chloroform isoamyl alcohol method was used for extracting DNA from blood. Previously published primers targeting cytochrome b gene sequence of Theileria annulata were used in the PCR assay that was standardized to use in the laboratory.

Results: Out of 29 samples tested,18 (62.06%) were found positive for theileriosis by PCR assay, whereas only 10 (34.48%) samples were detected positive by conventional microscopic technique using Giemsa staining method.

Conclusions: On the basis results of comparative studies, it can be concluded that PCR assay is a more sensitive than microscopic examination for detection of theileriosis. This can be attributed to the ability of PCR assay to detect small amounts of genomic DNA of T. annulata or low parasitemia in cows. Therefore, PCR assay can serve as a more sensitive tool to detect Theileria for detection of theileriosis even in asymptomatic carrier cattle which is important for the implementation of successful control programs.

Keywords: Carrier state, cytochrome b, Giemsa staining, polymerase chain reaction assay, theileriosis, Theileria annulata.

Molecular characterization of Fasciola hepatica and phylogenetic analysis based on mitochondrial (nicotiamide adenine dinucleotide dehydrogenase subunit I and cytochrome oxidase subunit I) genes from the North-East of Iran

Research (Published online: 29-09-2016)

20. Molecular characterization of Fasciola hepatica and phylogenetic analysis based on mitochondrial (nicotiamide adenine dinucleotide dehydrogenase subunit I and cytochrome oxidase subunit I) genes from the North-East of Iran - Saber Reaghi, Ali Haghighi, Majid Fasihi Harandi, Adel Spotin, Kourosh Arzamani and Soheila Rouhani

Veterinary World, 9(9): 1034-1038

   doi: 10.14202/vetworld.2016.1034-1038

Abstract

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Aim: Fascioliasis is one of the most zoonotic diseases with global extension. As the epidemiological distribution of Fasciola may lead to various genetic patterns of the parasite, the aim of this study is to identify Fasciola hepatica based on spermatogenesis, and phylogenetic analysis using mitochondrial (nicotiamide adenine dinucleotide dehydrogenase subunit I [ND1] and cytochrome oxidase subunit I) gene marker.

Materials and Methods: In this study, 90 F. hepatica collected from 30 cattle at slaughterhouse located in three different geographical locations in the North-East of Iran were evaluated based on spermatogenetic ability and internal transcribed spacer 1 gene restriction fragment length polymorphism pattern. Genetic diversity and phylogenetic relationship using mtDNA gene marker for the isolates from the North-East of Iran, and other countries were then analyzed.

Results: Partial sequences of mtDNA showed eight haplotypes in both genes. The phylogenic analysis using neighbor joining as well as maximum likelihood methods showed similar topologies of trees. Pairwise fixation index between different F. hepatica populations calculated from the nucleotide data set of ND1 gene are statistically significant and show the genetic difference.

Conclusion: F. hepatica found in this region of Iran has different genetic structures through the other Fasciola populations in the world.

Keywords: Cytochrome oxidase subunit I, Fasciola hepatica, Iran, molecular characterization, nicotiamide adenine dinucleotide dehydrogenase subunit I, phylogenic.

Seroprevalence of contagious ecthyma in goats of Assam: An analysis by indirect enzyme-linked immunosorbent assay

Research (Published online: 28-09-2016)

19. Seroprevalence of contagious ecthyma in goats of Assam: An analysis by indirect enzyme-linked immunosorbent assay - Mousumi Bora, Durlav Prasad Bora, Nagendra Nath Barman, Biswajyoti Borah and Sutopa Das

Veterinary World, 9(9): 1028-1033

   doi: 10.14202/vetworld.2016.1028-1033

Abstract

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Aim: The objective of this study was to screen the prevalence of contagious ecthyma (CE) among the goat population of Assam owing to its high prevalence rate.

Materials and Methods: In this study, a total of 231 serum samples were collected from 12 districts of Assam during September 2013 to July 2014. The serum samples were tested for the presence of antibodies against Orf virus (ORFV) by indirect enzyme-linked immunosorbent assay (ELISA). Indirect ELISA was standardized using purified Orf reference virus produced in bulk in primary lamb testes cells.

Results: Studies on seroprevalence showed 76.62% of goats were seropositive. The total number of animals were divided into different age groups starting from 0-2 months, 2-4 months, 4-6 months, and above 8 months and accordingly highest prevalence of antibodies against ORFV was recorded in the age-group above 8 months of age. Significantly, lower rates of infection were observed in goats of age group 2-4 months. This study recorded that seropositivity from naturally infected animals and in contact apparently healthy animals to be 53.67% and 46.32%, respectively.

Conclusion: The results indicated that CE is a prevalent infection in goats of Assam, and the healthy population is at increased risk of infection.

Keywords: Assam, contagious ecthyma, goats, indirect enzyme linked immuno sorbent assay, serosurveillance.

Detection of Peste des petits ruminants virus and goatpox virus from an outbreak in goats with high mortality in Meghalaya state, India

Research (Published online: 28-09-2016)

18. Detection of Peste des petits ruminants virus and goatpox virus from an outbreak in goats with high mortality in Meghalaya state, India - A. Karim, U. Bhattacharjee, K. Puro, I. Shakuntala, R. Sanjukta, S. Das, S. Ghatak and A. Sen

Veterinary World, 9(9): 1025-1027

   doi: 10.14202/vetworld.2016.1025-1027

Abstract

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Aim: We describe a laboratory investigation carried out to confirm the etiology of the heavy mortality (37 animals died out of total 44, i.e. 84%) in goats in Ri-Bhoi district of Meghalaya, Northeast region of India in December 2015. The clinical signs observed were abortion, diarrhea, high fever (up to 104°F), pox lesion in the skin, and respiratory distress.

Materials and Methods: The samples comprising whole blood, sera, and pox lesion were collected from the animals (n=7) from an outbreak for the screening of peste des petits ruminants (PPR) and poxviruses. The whole blood and sera were used for screening of PPR virus (PPRV) by sandwich enzyme-linked immunosorbent assay (ELISA) and antibody by competitive ELISA as well as detection of PPRV partial N gene by reverse transcription-polymerase chain reaction (PCR). The skin lesions were used for the detection of poxvirus by PCR.

Results: The results showed the presence of PPR antigens (58-80%) in the samples by sandwich ELISA and antibody in all the sera samples ranging from 9% to 41% positivity in competitive ELISA. Four samples were positive for PPRV partial N gene. The skin lesion screened for poxvirus was also found to be positive for I3L gene of goatpox virus.

Conclusion: We confirm the outbreak of disease in goats with high mortality is a case of mixed infection of PPR and goatpox detected for the first time in Northeast India.

Keywords: Capripoxvirus, disease, goat, mortality, Peste des petits ruminants.