How safe is the use of chlorpyrifos: Revelations through its effect on layer birds

Research (Published online: 22-07-2016)

13. How safe is the use of chlorpyrifos: Revelations through its effect on layer birds - P. P. Singh, Ashok Kumar, R. S. Chauhan and P. K. Pankaj

Veterinary World, 9(7): 753-758

   doi: 10.14202/vetworld.2016.753-758

Abstract

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Aim: The present study was aimed to investigate the immunological competence of chlorpyrifos (CPF) insecticide after oral administration in layer chickens.

Materials and Methods: A total of 20 White Leghorn birds were given CPF in drinking water at 0.3 ppm/bird/day (no observable effect level dose) for a period of 3-month. Immune competence status of layer birds and chicks hatched from CPF-treated birds were estimated at 15 days interval in layer birds and monthly interval in chicks using immunological and biochemical parameters.

Results: There was a significant decrease in values of total leukocytes count, absolute lymphocyte count, absolute heterophil count, total serum protein, serum albumin, serum globulin, and serum gamma globulin in the birds treated with CPF as compared to control. Similarly, immune competence tests such as lymphocyte stimulation test, oxidative burst assay, and enzyme-linked immunosorbent assay tests indicated lower immunity in birds treated with CPF as compared to control. Subsequently, chicks produced from CPF-treated birds were also examined for immune competence, but no significant difference was observed between chicks of both the groups.

Conclusion: The exposure to CPF produced hemo-biochemical and other changes that could be correlated with changes in the immunological profile of layer chickens suggesting total stoppage of using CPF in poultry sheds.

Keywords: blood biochemistry, chlorpyrifos, layers, immunity, organic pollutant.

A study on the tolerance level of farmers toward human-wildlife conflict in the forest buffer zones of Tamil Nadu

Research (Published online: 22-07-2016)

12. A study on the tolerance level of farmers toward human-wildlife conflict in the forest buffer zones of Tamil Nadu - K. Senthilkumar, P. Mathialagan, C. Manivannan, M. G. Jayathangaraj and S. Gomathinayagam

Veterinary World, 9(7): 747-752

   doi: 10.14202/vetworld.2016.747-752

Abstract

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Aim: The aim of this work was to study the tolerance level of farmers toward different human-wildlife conflict (HWC) situations.

Materials and Methods: This study was conducted in 24 villages of nine blocks from Kancheepuram, Coimbatore, Erode, and Krishnagiri districts of Tamil Nadu by personally interviewing 240 farmers affected with four different HWC situations such as human-elephant conflict (HEC), human-wild pig conflict (HPC), human-gaur conflict (HGC), and human-monkey conflict (HMC). A scale developed for this purpose was used to find out the tolerance level of the farmers.

Results: In general, the majority (61.70%) of the farmers had medium level of tolerance toward HWC, whereas 25.40% and 12.90% belonged to a high and low category, respectively. The mean tolerance level of the farmer’s encountering HMC is low (8.77) among the other three wild animal conflicts. In tackling HWC, the majority (55.00%) of the HEC farmers drove the elephant once it entered into their farmland. In the HPC, more than three-fourths of the respondents drove away the wild pig once they were found in farmlands. With regard to the HMC, a less number of them (1.70%) drove the monkey away if monkeys were spotted in their village. With regard to HGC, 95.00% of the respondents frightened the gaurs if their family members were threatened by gaurs.

Conclusion: The present study suggests that that majority of the farmers had medium level of tolerance toward HWC. The tolerance level of the HMC farmers was lower than other three HWC affected farmers. This study emphasizes the need for necessary training to tackle the problem in an effective manner for wild animal conservation.

Keywords: elephant, gaur, human-wildlife conflict, local perceptions, monkey, tolerance level, wild pig.

Seroprevalence of human brucellosis in and around Jammu, India, using different serological tests

Research (Published online: 17-07-2016)

11. Seroprevalence of human brucellosis in and around Jammu, India, using different serological tests - H. K. Sharma, S. K. Kotwal, D. K. Singh, M. A. Malik, Arvind Kumar, Rajagunalan and M. Singh

Veterinary World, 9(7): 742-746

   doi: 10.14202/vetworld.2016.742-746

Abstract

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Aim: Brucellosis is a disease of zoonotic importance as it affects both human as well as animal’s health, and therefore, directly affects animal productivity and human efficiency. Therefore, a study was conducted to estimate the seroprevalence of brucellosis in humans in Jammu and surrounding areas.

Materials and Methods: A total of 121 sera samples from humans occupied with professional related to animals were collected and tested for anti-Brucella antibodies by Rose Bengal plate test (RBPT), modified RBPT (mRBPT), standard tube agglutination test (STAT), and indirect enzyme-linked immunosorbent assay (I-ELISA). Sampling was done keeping in view with the occupation, sex, and age.

Results: The overall seroprevalence of brucellosis recorded was 4.96%. The test-wise seroprevalence was 9.91% by RBPT, 9.91% by mRBPT, 9.09% by STAT, and 16.52% by I-ELISA. The prevalence of brucellosis was higher in >35-50 years age group compared to >20-35 years and >50-65 years. Sex-wise seroprevalence was higher in males than females. Taking I-ELISA as standard, the relative sensitivities of mRBPT, RBPT, and I-ELISA were in the order of mRBPT=RBPT>STAT. All the tests revealed high specificity values; however, among different serological tests, I-ELISA detected a maximum number of positive sera samples.

Conclusions: The prevalence of brucellosis was found to be approximately 5%. The adult (>35-50 years) age male group was most vulnerable. The routine diagnosis of brucellosis involved the conventional serological tests, viz., RBPT and STAT, but each was associated with drawbacks which could give either false-positive or false-negative interpretation. Therefore, it is always recommended to use a battery of tests in the diagnosis of brucellosis.

Keywords: brucellosis, humans, indirect enzyme-linked immunosorbent assay, Rose Bengal plate test, seroprevalence, standard tube agglutination test.

Immuno-pathological studies on broiler chicken experimentally infected with Escherichia coli and supplemented with neem (Azadirachta indica) leaf extract

Research (Published online: 16-07-2016)

10. Immuno-pathological studies on broiler chicken experimentally infected with Escherichia coli and supplemented with neem (Azadirachta indica) leaf extract - Vikash Sharma, K. K. Jakhar and Swati Dahiya

Veterinary World, 9(7): 735-741

   doi: 10.14202/vetworld.2016.735-741

Abstract

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Aim: The present study was conducted to evaluate the effects of neem leaf extract (NLE) supplementation on immunological response and pathology of different lymphoid organs in experimentally Escherichia coli challenged broiler chickens.

Materials and Methods: For this study, we procured 192-day-old broiler chicks from local hatchery and divided them into Groups A and Group B containing 96 birds each on the first day. Chicks of Group A were supplemented with 10% NLE in water, whereas chicks of Group B were not supplemented with NLE throughout the experiment. At 7th day of age, chicks of Group A were divided into A1 and A2 and Group B into B1 and B2 with 54 and 42 chicks, respectively, and chicks of Groups A1 and B1 were injected with E. coli O78 at 107colony-forming units/0.5 ml intraperitoneally. Six chicks from each group were sacrificed at 0, 2, 4, 7, 14, 21, and 28 days post infection; blood was collected and thorough post-mortem examination was conducted. Tissue pieces of spleen and bursa of Fabricius were collected in 10% buffered formalin for histopathological examination. Serum was separated for immunological studies.

Result: E. coli specific antibody titer was significantly higher in Group A1 in comparison to Group B1. Delayed-type hypersensitivity response against 2,4 dinirochlorobenzene (DNCB) antigen was significantly higher in Group A1 as compared to Group B1. Pathological studies revealed that E. coli infection caused depletion of lymphocytes in bursa of Fabricius and spleen. Severity of lesions in Group A1 was significantly lower in comparison to Group B1.

Conclusion: 10% NLE supplementation enhanced the humoral as well as cellular immune responses attributed to its immunomodulatory property in experimentally E. coli infected broiler chicken.

Keywords: broiler chicken, Escherichia coli, immune response, neem leaf extract.

Prevalence and risk factors associated with gastrointestinal nematode infection in goats raised in Baybay city, Leyte, Philippines

Research (Published online: 14-07-2016)

9. Prevalence and risk factors associated with gastrointestinal nematode infection in goats raised in Baybay city, Leyte, Philippines - Ariel Paul M. Rupa and Harvie P. Portugaliza

Veterinary World, 9(7): 728-734

   doi: 10.14202/vetworld.2016.728-734

Abstract

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Aim: Gastrointestinal parasitism is a serious constraint affecting goat production in the Philippines. This study aimed to determine the prevalence and associated risk factors of gastrointestinal nematode infection in goat-populated barangays of Baybay City, Leyte.

Materials and Methods: A total of 81 households or farms were interviewed, and 450 goats were sampled for fecalysis. Fecal egg count along with egg morphological identification and coproculture for third stage larvae identification were conducted. Descriptive statistics and logistic regression analyses were carried out to determine the farm- and animal-level prevalence and risk factors.

Results: Fecalysis revealed the presence of strongyle and Trichuris spp. with a farm-level prevalence of 100% and 4.94%, respectively; and animal-level prevalence of 96.22% and 4.44%, respectively. The identified strongyle genera per barangay were Haemonchus spp. (34.79%), Trichostrongylus spp. (33.29%), Oesophagostomum spp. (24.21%), Cooperia spp. (6.93%), and Chabertia spp. (0.79%). Goats older than 12 months were four times more likely to present high strongyle burden when compared to goats <6 months. With each month increase in goat’s age, the odds of acquiring strongyle infection also increased by 1.07 times. Animals kept in goat house with cemented flooring have lower odds of acquiring strongyle (odds ratio=0.12). Goats raised for leisure purposes and fed with carabao grass (Paspalum conjugatum) were 8.12 and 5.52 times more likely to acquire Trichuris, respectively.

Conclusion: Most of the backyard goat raisers in Baybay City, Leyte, do not practice sound helminth control measures as shown by the high prevalence of gastrointestinal nematodes. The most relevant risk factors for gastrointestinal nematode infection were the age of the goat, type of goat house’s flooring, purpose of raising goats, and feeding practices.

Keywords: goat, Haemonchus, Philippines, risk factors, strongyle, Trichuris.

Effect of feeding cottonseed meal on some hematological and serum biochemical parameters in broiler birds

Research (Published online: 13-07-2016)

8. Effect of feeding cottonseed meal on some hematological and serum biochemical parameters in broiler birds - G. Thirumalaisamy, M. R. Purushothaman, P. Vasantha Kumar and P. Selvaraj

Veterinary World, 9(7): 723-727

   doi: 10.14202/vetworld.2016.723-727

Abstract

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Aim: The study was undertaken to find out the effect of feeding cottonseed meal (CSM) on performance of hematological and serum biochemical parameters in broiler birds.

Materials and Methods: A 6-week biological trial was carried out with 432-day-old Cobb 400 broiler chicks distributed to nine experimental diets with six replicates, each containing eight chicks. The experimental diets were formulated based on total amino acids (BTAA) or based on digestible amino acids (BDAA) with or without iron supplementation with two levels of CSM (2% and 4%) and control diet based on maize – soybean. The whole blood was subjected to hematological studies. The serum samples were analyzed for protein fractions and lipid profiles.

Results: The packed cell volume (PCV) value, red blood cell (RBC) numbers, and hemoglobin (Hb) were lower in iron unsupplemented CSM BTAA or BDAA diets than the control (33.86-35.54 vs. 36.41%, 2.78-2.87 vs. 2.98 × 10⁶/μl, and 10.30-10.70 vs. 10.88%). Supplementation of iron in CSM diets improved the PCV, RBC numbers, and Hb, and the values were comparable to the control. White blood cell numbers, mean corpuscular volume, mean corpuscular Hb (MCH), and MCH concentration values were comparable to the control. The erythrocyte osmotic fragility (EOF) was poor in birds fed diets containing up to 4% CSM BTAA or CSM BDAA without iron supplementation (32.02-32.57 vs. 28.77%). Supplementation of iron improved the EOF. The serum cholesterol level did not change with or without iron supplementation.

Conclusion: This study suggested that feeding of CSM BTAA or BDAA up to 4% level voiding iron supplementation lowers the hematological parameters, whereas supplementation of iron did not alter serum protein fractions and cholesterol profile; however, it had lowered some hematological parameters, which was rectified by iron supplementation.

Keywords: broiler, cottonseed meal, hematological and serum parameters, iron supplementation.

A comparison of titers of anti-Brucella antibodies of naturally infected and healthy vaccinated cattle by standard tube agglutination test, microtiter plate agglutination test, indirect hemagglutination assay, and indirect enzyme-linked immunosorbent assay

Research (Published online: 12-07-2016)

7. A comparison of titers of anti-Brucella antibodies of naturally infected and healthy vaccinated cattle by standard tube agglutination test, microtiter plate agglutination test, indirect hemagglutination assay, and indirect enzyme-linked immunosorbent assay - Anju Mohan, Hari Mohan Saxena and Puneet Malhotra

Veterinary World, 9(7): 717-722

   doi: 10.14202/vetworld.2016.717-722

Abstract

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Aim: We determined the antibody response in cattle naturally infected with brucellosis and normal healthy adult cattle vaccinated during calf hood with strain 19.

Materials and Methods: The antibody titers were measured by standard tube agglutination test (STAT), microtiter plate agglutination test (MAT), indirect hemagglutination assay (IHA), and indirect enzyme-linked immunosorbent assay (iELISA) as per standard protocols.

Results: The mean STAT titers were 1.963±0.345 in infected cattle and 1.200±0.155 in healthy vaccinated cattle. The difference was extremely significant (p<0.0001). The mean MAT titers were 2.244±0.727 in infected cattle and 1.200±0.155 in healthy vaccinated cattle. The difference was very significant (p<0.005). The mean IHA titers in infected cattle were 2.284±0.574, and those in healthy vaccinated cattle were 1.200±0.155. The difference was extremely significant (p=0.0002). However, the difference in mean iELISA titers of infected cattle (1.3678±0.014) and healthy vaccinated cattle (1.367±0.014) was non-significant. The infected animals showed very high titers of agglutinating antibodies compared to the vaccinated animals. However, it cannot be ascertained whether these antibodies are due to vaccine or response to infection. Since the infected animals had been vaccinated earlier, the current infection may suggest that vaccination was unable to induce protective levels of antibody. The heightened antibody response after infection may also indicate a secondary immune response to the antigens common to the vaccine strain and wild Brucella organisms.

Conclusion: The brucellosis infected animals showed very high titers of agglutinating antibodies compared to the vaccinated animals.

Keywords: antibody titers, Brucella, brucellosis, Brucella abortus S19 vaccine, bovine brucellosis.