Molecular characterization of exon 3 of caprine myostatin gene in Marwari goat

Research (Published online: 30-06-2016)

22. Molecular characterization of exon 3 of caprine myostatin gene in Marwari goat - Jai Prakash Khichar, Gyan Chand Gahlot, Vijay Kumar Agrawal, Kiran, Ajay Singh Dewna, Prakash and Mohammad Ashraf

Veterinary World, 9(6): 676-679

   doi: 10.14202/vetworld.2016.676-679

Abstract

---

Aim: To estimate genetic variability in exon 3 of caprine myostatin gene in Marwari goats.

Materials and Methods: A total of 120 blood samples from unrelated Marwari goats were randomly collected from different villages of Bikaner (Rajasthan), India. Genomic DNA was extracted from whole blood using blood DNA isolation kit (Himedia Ltd.) as per manufacturer’s protocol. The quality of extracted genomic DNA was checked on 0.8% agarose gel. Specifically designed a primer set for caprine myostatin (MSTN) gene (Genebank accession no. DQ167575) was used to amplify the exon 3 region of MSTN gene in Marwari goat. The genetic variability in exon 3 of MSTN gene in Marwari goat was assessed on 8% polyacrylamide gel electrophoresis to detect single strand conformation polymorphism (SSCP) pattern.

Results: The exon 3 of MSTN gene in Marwari goat showed two types of conformation patterns on 8% polyacrylamide gel. One of the patterns showed only two bands and was considered as genotype AA, whereas another pattern having an extra band was designated as genotype AB. The frequencies of AA and AB genotype for exon 3 region of MSTN gene were calculated as 0.90 and 0.10, respectively.

Conclusion: Low level of polymorphism was observed at exon 3 region of MSTN gene in Marwari goat through SSCP analysis. This information could be utilized in future breeding plan to exploit the unique characteristics of Marwari goat of Rajasthan.

Keywords: exon 3, Marwari goat, myostatin gene, polymerase chain reaction-single strand conformation polymorphism.

Genetic analysis of first lactation production traits in Kankrej cattle

Research (Published online: 29-06-2016)

21. Genetic analysis of first lactation production traits in Kankrej cattle - K. J. Ankuya, N. K. Pareek, M. P. Patel, B. S. Rathod, K. B. Prajapati and J. B. Patel

Veterinary World, 9(6): 672-675

   doi: 10.14202/vetworld.2016.672-675

Abstract

---

Aim: The aim was to estimate genetic factors affecting the first lactation milk production traits in Kankrej cattle of North Gujarat.

Materials and Methods: The 475 first lactation records of Kankrej cows that were maintained at the Livestock Research Station, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat, over a period of 35 years from 1980 to 2014 were studied. The least squares maximum likelihood program was used to estimate genetic parameters of first lactation traits. Heritability was estimated through paternal half-sib analysis in adjusted data.

Results: The heritability estimate for production traits was 0.40±0.17, 0.45±0.17, 0.35±0.18, and 0.20±0.14 for standard 300 days milk yield (F300Y), total lactation milk yield (FLY), wet average (FWA), and lactation length (FLL), respectively, in the first parity. All the genetic and phenotypic correlations among different production efficiency traits were high and positive. Genetic correlations between F300Y and FLY, FLL, and FWA were 0.80±0.20, 0.59±0.16, and 0.81±0.32,where as the phenotypic correlations were 0.969, 0.688, and 0.868, respectively. Genetic correlations of FLY with FLL and FWA were 0.60±0.13 and 0.79±0.20, whereas the phenotypic correlations were 0.777 and 0.817, respectively. Genetic and phenotypic correlation between FLL and FWA was 0.63±0.28 and 0.31, respectively.

Conclusion: The heritability estimate of all first parity lactation traits waslow to medium (0.20-0.45) indicated the scope for further improvement in this trait through selection as well as managemental practice. Higher genetic and phenotypic correlation between thefirst lactation milk production traits gives theidea that genetic gain due to selection for one trait also givesmorecorrelated response of selection for other traits which is economically advantageous.

Keywords: correlation, heritability, first parity, Kankrej cattle.

The detrimental effects of lead on human and animal health

Review (Published online: 27-06-2016)

20. The detrimental effects of lead on human and animal health - Mohammed Abdulrazzaq Assi, Mohd Noor Mohd Hezmee, Abd Wahid Haron, Mohd Yusof Mohd Sabri and Mohd Ali Rajion

Veterinary World, 9(6): 660-671

   doi: 10.14202/vetworld.2016.660-671

Abstract

---

Lead, a chemical element in the carbon group with symbol Pb (from Latin: Plumbum, meaning “the liquid silver”) and has an atomic number 82 in the periodic table. It was the first element that was characterized by its kind of toxicity. In animal systems, lead (Pb) has been incriminated in a wide spectrum of toxic effects and it is considered one of the persistent ubiquitous heavy metals. Being exposed to this metal could lead to the change of testicular functions in human beings as well as in the wildlife. The lead poising is a real threat to the public health, especially in the developing countries. Accordingly, great efforts on the part of the occupational and public health have been taken to curb the dangers of this metal. Hematopoietic, renal, reproductive, and central nervous system are among the parts of the human body and systems that are vulnerable toward the dangers following exposure to high level of Pb. In this review, we discussed the massive harmful impact that leads acetate toxicity has on the animals and the worrying fact that this harmful toxicant can be found quite easily in the environment and abundance. Highlighting its (Pb) effects on various organs in the biological systems, its economic, as well as scientific importance, with the view to educate the public/professionals who work in this area. In this study, we focus on the current studies and research related to lead toxicity in animals and also to a certain extent toward human as well.

Keywords: antioxidant, free radical, lead poisoning, oxidative stress.

Prevalence of coccidiosis among village and exotic breed of chickens in Maiduguri, Nigeria

Research (Published online: 25-06-2016)

19. Prevalence of coccidiosis among village and exotic breed of chickens in Maiduguri, Nigeria - Jallailudeen Rabana Lawal, Saleh Mohammed Jajere, Umar Isa Ibrahim, Yaqub Ahmed Geidam, Isa Adamu Gulani, Gambo Musa and Benjamin U. Ibekwe

Veterinary World, 9(6): 653-659

   doi: 10.14202/vetworld.2016.653-659

Abstract

---

Aim: Coccidiosis is an important enteric parasitic disease of poultry associated with significant economic losses to poultry farmers worldwide. This survey was conducted from June 2014 through July 2015 with the main goal of investigating the prevalence and associated risk factors of coccidiosis among village and exotic breeds of chickens in Maiduguri, Northeastern Nigeria.

Materials and Methods: A total of 600 fecal samples from live and slaughtered birds comprising 284 young, 141, growers and 175 adult birds; 379 male and 221 female birds; 450 exotic and 150 local breeds of birds were randomly collected either as bird’s fresh droppings or cutting open an eviscerated intestine of slaughtered birds, while noting their age, sex, and breeds. Samples were analyzed using standard parasitological methods and techniques.

Results: An overall prevalence rate of 31.8% (95% confidence interval: 28.07-35.52) was obtained. Higher prevalence rates were recorded in growing birds 58.9% (50.78-67.02), female birds 35.3% (29.00-41.60), exotic birds 42.4% (37.83- 46.97), and broiler birds 68.7% (61.28-76.12). Similarly, higher infection rates were also observed among birds sampled from Mairi ward 66.7% (56.03-77.37), intensive management system 46.5% (41.61-51.39), and constructed local cages 54.0% (46.02-61.98). The difference in prevalence of coccidiosis among age groups, breeds, among exotic breeds, sampling locations, husbandry management systems, and litter management systems was statistically significant (<0.0001). However, no significant difference (p>0.05) of infection rates was observed in sex.

Conclusion: Coccidiosis is endemic in both commercial and backyard poultry farms in Maiduguri due to poor management practices encouraging Eimeria oocysts build-up. It is therefore, recommended that poultry farmers should practice strict biosecurity measures on their farms, creating awareness on the prevalence of coccidiosis, routine vaccination against coccidiosis and educating poultry farmers on the need for maintaining good hygienic standards and good flock health management.

Keywords: coccidiosis, exotic breeds, Maiduguri, Northeastern Nigeria, prevalence, village chickens.

Optimization of dry period in Karan Fries cow

Research (Published online: 24-06-2016)

18. Optimization of dry period in Karan Fries cow - K. Puhle Japheth, R. K. Mehla, Mahendra Singh, A. K. Gupta, Ramendra Das, Pranay Bharti and T. Chandrasekar

Veterinary World, 9(6): 648-652

   doi: 10.14202/vetworld.2016.648-652

Abstract

---

Aim: The objective of this study was to optimize dry period (DP) length that can maximize the production across adjacent lactations and overall lifetime yield.

Materials and Methods: Performance records with respect to DP spread over a period of 15-year in Karan Fries (KF) cattle maintained at Livestock Research Centre (National Dairy Research Institute), were collected for the study. Data of 681 KF cows were analyzed by least square technique to examine the effect of non-genetic factors on DP. Season of calving was classified into four seasons: Winter season (December-March), summer season (April-June), rainy season (July-September), and autumn season (October-November); period of calving into five periods: 1998-2000 (1-period), 2001-2003 (II-period), 2004-2006 (III-period), 2007-2009 (IV-period), and 2010-2012 (V-period), and parity into six parities, i.e., 1st, 2nd, 3rd, 4th, 5th, and ˃6th parities to see the effect of non-genetic factors on DP.

Results: Period of calving, season of calving, and parity did not affect the DP significantly (p<0.05). The overall least square mean of DP was 67.93±2.12 days. For the optimization of DP with regard to milk productivity, analysis was carried out by class interval method. DP was classified into eight classes (˂22, 23-45, 46-67, 68-89, 90-111, 112-133, 134-155, and ˃156 days), and optimum level was obtained at 46-67 days (3rd class) with the following respective milk yield (MY) of 305 daily MY (4016.44±43.68 kg), total MY (4704.21±61.51 kg), MY per day of lactation length (13.03±0.13 kg), and MY per day of calving interval (11.68±0.41 kg).

Conclusion: From the study, it was concluded that this optimal DP length (46-67 days) is suitable for maximizing the production. Hence, one should aim to dry off pregnant cows to achieve a DP of appropriate length to enhance productivity in the next lactation, as very short and very long DP reduces the economic profitability in dairy animals.

Keywords: dry period, economic trait, Karan Fries cow, non-genetic factors, optimization.

Immunotoxicological, biochemical, and histopathological studies on Roundup and Stomp herbicides in Nile catfish (Clarias gariepinus)

Research (Published online: 23-06-2016)

17. Immunotoxicological, biochemical, and histopathological studies on Roundup and Stomp herbicides in Nile catfish (Clarias gariepinus) - Gihan G. Moustafa, F. E. Shaaban, A. H. Abo Hadeed, and Walaa M. Elhady

Veterinary World, 9(6): 638-647

   doi: 10.14202/vetworld.2016.638-647

Abstract

---

Aim: The current study was directed to investigate the immunotoxic and oxidative stress effects of Roundup and Stomp herbicides and their combination on Nile catfish (Clarias gariepinus).

Materials and Methods: The experiment was carried out on 120 fish that randomly divided into four equal groups with three replicates: The first group kept as control, the second group exposed to 1/2 96 h lethal concentration 50 (LC₅₀) of Roundup, the third group exposed to 1/2 96 h LC₅₀ of Stomp, and the fourth one exposed to a combination of Roundup and Stomp at previously-mentioned doses. The experiment was terminated after 15 days; blood samples were obtained at 1st, 8th, and 15th days of treatment where the sera were separated for estimation of antioxidant enzymes. Meanwhile, at 15th day of exposure part of blood was collected from all groups with an anticoagulant for evaluation of phagocytic activity, then the fish were sacrificed, and specimens from the liver of all groups were obtained for histopathological examination.

Results: Our results indicated that both herbicides either individually or in combination elucidated significant decrease in phagocytic activity that was highly marked in group exposed to both herbicides. Furthermore, our data elicited an obvious elevation in the levels of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). Meanwhile, the data depicted reduction in levels of reduced glutathione (GSH) and glutathione-S-transferase (GST). Histopathological investigation of liver proved the aforementioned results.

Conclusion: It could be concluded that either Roundup or Stomp alone cause significant deleterious effects on aquatic vertebrates. However, the use of their combination enhanced their toxic effects. Toxicity can end up in humans through the food chain.

Keywords: antioxidant enzymes, fish, phagocytosis, Roundup, Stomp.

Prevalence, type, and prognosis of ocular lesions in shelter and ownedclient dogs naturally infected by Leishmania infantum

Research (Published online: 22-06-2016)

16. Prevalence, type, and prognosis of ocular lesions in shelter and ownedclient dogs naturally infected by Leishmania infantum - Simona Di Pietro, Valentina Rita Francesca Bosco, Chiara Crinò, Francesco Francaviglia and Elisabetta Giudice

Veterinary World, 9(6): 633-637

   doi: 10.14202/vetworld.2016.633-637

Abstract

---

Aim: The point prevalence of ocular lesions due to leishmaniasis was evaluated in 127 dogs living in a municipal shelter placed in a highly endemic area (Sicily, Italy). Moreover, the period prevalence, the type, and prognosis of lesions due to leishmaniasis were evaluated in 132 dogs with ocular pathologies referred to a Veterinary Teaching Hospital (VTH) in the same endemic area over a 3-year period.

Materials and Methods: All the dogs were submitted to ophthalmological examination. The diagnosis of leishmaniasis was made by cytological, serological (immune-fluorescent antibody test), and molecular (quantitative polymerase chain reaction) tests.

Results: The point prevalence of ocular lesions in 45 shelter dogs with leishmaniasis was 71.11% (45/127 dogs). The most frequent ocular lesion was blepharitis (50%) while anterior uveitis was observed in only 9.37% of cases. The period prevalence of ocular lesions due to leishmaniasis in the VTH group was 36.36% (48/132 dogs). In both groups, most of the lesions were bilateral and involved the anterior segment. Anterior uveitis was the most frequent ophthalmic finding in client-owned dogs (37.50%), but it occurred in only 9.37% of the shelter dogs. Keratouveitis often occurred during or after antiprotozoal treatment (14.58%; 7/48). In this study, the healing of eye injury following systemic antiprotozoal treatment was recorded in about half of cases (48%; 12/25 dogs), in which follow-up was possible. In more than 1/3 of cases (36%; 9/25), there was an improvement, but it was necessary to associate a long-term topical treatment; most of them, as well as those who had not responded to systemic therapy (16%; 4/25), had anterior uveitis or keratoconjunctivitis sicca.

Conclusions: Ocular manifestations involve up to 2/3 of animals affected by canine leishmaniasis and lesions account for over 1/3 of ophthalmic pathologies observed at a referral clinic in an endemic area. The occurrence of anterior uveitis is more frequent in client-owned dogs than in shelter dogs. The onset of keratouveitis during or after antiprotozoal treatment could be attributed to the treatment or to a recurrence of the systemic form. The post-treatment uveal immune reaction, already observed in humans, could explain the difference in the frequency of keratouveitis between client-owned and shelter dogs, which have never been treated.

Keywords: dog, follow-up, leishmaniasis, ocular lesions, post-treatment uveitis.