The structural and functional recovery of pancreatic β-cells in type 1 diabetes mellitus induced mesenchymal stem cell-conditioned medium

Research (Published online: 29-05-2016)

18. The structural and functional recovery of pancreatic β-cells in type 1 diabetes mellitus induced mesenchymal stem cell-conditioned medium - Widagdo Sri Nugroho, Dwi Liliek Kusindarta, Heru Susetya, Ida Fitriana, Guntari Titik Mulyani, Yuda Heru Fibrianto, Aris Haryanto and Teguh Budipitojo

Veterinary World, 9(5): 535-539

   doi: 10.14202/vetworld.2016.535-539

Abstract

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Aim: Various studies have shown that secreted factors alone in culture medium without stem cell are capable of repairing tissues by itself in various conditions involving damaged tissue/organ. Therefore, this study was aimed to investigate the role of human umbilical cord mesenchymal stem cell-derived conditioned medium (CM) on the recovery of pancreatic β-cells in Wistar rats (Rattus norvegicus) with type 1 diabetes mellitus.

Materials and Methods: The 0.05 ml CM induction was applied to the diabetic group of rats in weeks 1, 2, 3, and 4. 1 week after each CM induction, insulin concentration was analyzed using ELISA. The pancreas was divided into 3 regions, processed by paraffin method, stained with hematoxylin-eosin, and immunohistochemical method for insulin.

Results: This study indicated the decrease in the total number of islets and insulin concentration after the injection of single dose of alloxan. The exocrine acini were also damaged. Microscopic observation detected the presence of small islets in the diabetic group 1 week after the first 0.05 ml CM induction. The number and size of the islets increased in line with the CM doses and time of inductions. Immunohistochemically, the presence of low intensity of insulin-positive cells could be recognized at the splenic and duodenal regions of the pancreas, but not gastric region, 1 week after the first and second 0.05 ml CM induction. The intensity of staining and the number of insulin-positive cells increased dramatically in 1 week after the third and fourth 0.05 ml of CM induction in all regions of the pancreas. The data of insulin blood concentration showed clear differences between the second and the fourth induction of 0.05 ml CM induction.

Conclusions: This study showed very strong evidence on the role of human umbilical cord mesenchymal stem cell-derived CM in recovering the pancreatic β-cells damage in Wistar rats (R. norvegicus) with type 1 diabetes mellitus, structurally and functionally.

Keywords: conditioned-medium, pancreatic β-cells, structural and functional recovery, type 1 diabetes mellitus.

Influence of season and sex on hemato-biochemical traits in adult turkeys under arid tropical environment

Research (Published online: 28-05-2016)

17. Influence of season and sex on hemato-biochemical traits in adult turkeys under arid tropical environment - Anil Gattani, Arti Pathak, Ajeet Kumar, Vaibhav Mishra and Jitendra Singh Bhatia

Veterinary World, 9(5): 530-534

   doi: 10.14202/vetworld.2016.530-534

Abstract

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Aim: The objective of this study was to evaluate the effect of season and sex on hemato-biochemical parameters of turkey (Meleagris gallopavo) in the arid tropical environment.

Materials and Methods: The experiment was conducted on 20-week old turkeys consisting of 20 males and 20 females. Blood was collected from all turkeys during January and May. Hemoglobin (Hb), red blood cell (RBC), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were estimated in whole blood and glucose, protein, albumin, globulin, A/G ratio, calcium, phosphorus, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in serum.

Result: Season has significant (p<0.05) effect on Hb concentration, RBC, and PCV in both male and female. Male has significantly higher (p<0.05) Hb concentration, RBC, and PCV. There is no significant effect of sex, and season was observed on MCV, MCH, and MCHC. Glucose, protein, albumin, globulin, and A/G ratio were significantly (p<0.05) affected by season and sex. AST and ALT were significantly (p<0.05) affected by season in both sexes. There is no significant difference was recorded on calcium, phosphorus due to season and sex.

Conclusion: Under arid tropical environment, turkey hemato-biochemical parameters are influenced by both sex and season.

Keywords: alanine aminotransferase, aspartate aminotransferase, season, sex, turkey.

Comparative efficacy of anthelmintics and their effects on hematobiochemical changes in fasciolosis of goats of South Gujarat

Research (Published online: 28-05-2016)

16. Comparative efficacy of anthelmintics and their effects on hematobiochemical changes in fasciolosis of goats of South Gujarat - R. G. Shrimali, M. D. Patel and R. M. Patel

Veterinary World, 9(5): 524-529

   doi: 10.14202/vetworld.2016.524-529

Abstract

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Aim: Fasciolosis is a parasitic disease caused by Fasciola spp. of the family Fasciolidae (trematodes) characterized by bottle jaw, anemia, progressive debility, and potbelly condition. There are many aspects of fasciolosis remaining unknown thus hemato-biochemical alterations in closantel, triclabendazole + ivermectin, and oxyclozanide + levamisole treated goats were studied.

Materials and Methods: A total of 40 naturally fasciolosis infected goats having egg per gram more than 100 were randomly divided into four groups. Goats of Group I-III were treated with three different anthelmintics, whereas, goats of Group-IV were kept as control or untreated. Whole blood, serum, and fecal samples were collected on 0, 7^th, and 30^th day of treatment.

Results: During the study, values of hemoglobin, total erythrocyte count, pack cell volume, and total protein were significantly elevated to their normal levels in anthelmintics treated groups. Whereas, values of total leukocyte count, aspartate transaminase (AST), lactate dehydrogenase (LDH), and gamma-glutamyl transferase (GGT) were significantly reduced to their normal level in anthelmintics treated groups. The efficacy of closantel (T1), triclabendazole + ivermectin (T2), and oxyclozanide + levamisole (T3) was 99.63%, 100%, and 94.74% and 100%, 100%, and 97.38% on 7^th and 30^th day of treatment, respectively.

Conclusions: Fasciolosis in goats can be diagnosed on the basis of fecal sample examination, but alterations in important biomarkers such as AST, GGT, and LDH are also helpful for early diagnosis. The use of newer anthelmintic either alone or in combination showed a higher therapeutic response in fasciolosis of goats.

Keywords: anthelmintics, efficacy, fasciolosis, goats, hemato-biochemical changes.

Effect of washing on the post-thaw quality of cryopreserved ram epididymal spermatozoa

Research (Published online: 27-05-2016)

15. Effect of washing on the post-thaw quality of cryopreserved ram epididymal spermatozoa - Touqeer Ahmed, Rafiqul Islam, Farooz Ahmad Lone and Asloob Ahmad Malik

Veterinary World, 9(5): 519-523

   doi: 10.14202/vetworld.2016.519-523

Abstract

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Aim: The aim of the study was to evaluate the effect of washing on the post-thaw quality of ram cauda epididymal spermatozoa (P1: Unwashed, P2: Washed).

Materials and Methods: Fresh testicles of adult healthy slaughtered rams were collected and transported to the laboratory in an ice chest, where they were weighed, and cauda epididymides were separated. These cauda epididymides were used for recovery of spermatozoa in tris-citric acid fructose buffer by incision method. Spermatozoa samples showing ≥70% progressive motility were pooled and processed further. The mean values (±standard error) of various parameters such as the percentage of sperm motility, live sperm, intact acrosome, and hypo-osmotic swelling test (HOST) rea cted spermatozoa were recorded.

Results: In this experiment, the percent sperm motility, live spermatozoa, and intact acrosome both at pre-freeze and postthaw were higher (p>0.05) in P1 than P2. However, the post-thaw percent HOST reacted spermatozoa was slightly higher (p>0.05) for P2 than P1.

Conclusion: Washing of cauda epididymal spermatozoa has no significant adverse effect on the quality during cryopreservation. Therefore, this processing method can be applied wherever necessary before the extension of the recovered spermatozoa sample in different ram extenders.

Keywords: cauda epididymis, centrifugation, processing, ram, spermatozoa, washing.

Reproductive disorders in dairy cattle under semi-intensive system of rearing in North-Eastern India

Research (Published online: 26-05-2016)

14. Reproductive disorders in dairy cattle under semi-intensive system of rearing in North-Eastern India - M. H. Khan, K. Manoj and S. Pramod

Veterinary World, 9(5): 512-518

   doi: 10.14202/vetworld.2016.512-518

Abstract

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Aim: This study was conducted to determine the incidence of major reproductive problems of dairy cattle reared under a semi-intensive system by small and marginal farmers in Meghalaya province of North-Eastern India.

Materials and Methods: In a 3 years study, a total of 576 crossbred dairy cattle (212 Holstein Friesian cross and 364 Jersey cross) from all districts (n=11) of Meghalaya were assessed with the survey, clinical examination, and personal observations.

Results: Out of the total animal assessed, 33.85% (n=195) were found to be affected with one or more of the clinical reproductive problems. Repeat breeding (RB), anestrus, retention of fetal membrane, and abortion were found to be the major clinical reproductive problems. Out of the total animal affected with reproductive disorders, the incidence of anestrus, RB, retention of fetal membrane, and abortion was found to be 31.79% (n=62), 24.61% (n=48), 14.35% (n=28), and 11.25% (n=22), respectively. In addition, dystocia (5.12%), prolapse (1.53%), endometritis (4.61%), and pyometra (6.66%) were minor clinical reproductive problems. There was a significant difference in the incidence of reproductive disorders with respect to breed, age, and parity.

Conclusion: It was revealed from this study that RB, anestrus, retention of fetal membrane, and dystocia are the major clinical reproductive problems in Meghalaya. Results indicated unsatisfactory feeding, housing, and health management practices are the main cause of low fertility of dairy cows. Lack of scientific knowledge, low access to breeding, and health services further contributed to low productivity and fertility.

Keywords: anestrus, dairy, infertility, Meghalaya, reproductive disorders.

Studies of the macroscopic and microscopic morphology (hippocampus) of brain in Vencobb broiler

Research (Published online: 24-05-2016)

13. Studies of the macroscopic and microscopic morphology (hippocampus) of brain in Vencobb broiler - Shailesh Kumar Gupta, Kumaresh Behera, C. R. Pradhan, Arun Kumar Mandal, Kamdev Sethy, Dayanidhi Behera and Kuladip Prakash Shinde

Veterinary World, 9(5): 507-511

   doi: 10.14202/vetworld.2016.507-511

Abstract

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Aim: The aim of this study was to study the anatomy of different parts of brain and histology of hippocampus of Vencobb broiler chicken.

Materials and Methods: A 12 adult experimental birds were sacrificed by cervical dislocation. After separation of the brain, gross anatomy features were studied. Brain tissue was fixed in 10% buffered neutral formalin for 2-3 days, and then routine dehydration process in ascending grades of ethyl alcohol was done. After xylene cleaning, paraffin impregnation was prepared. Paraffin blocks were cut, and slides were stained by Harris hematoxylin and eosin. Photography was carried out both under lower (×10) and higher (×40) magnifications.

Results: The brain structure (dorsal view) of Vencobb bird resembled the outline of a playing card symbol of a “spade.” The brain subdivisions are cerebrum, cerebellum, and medulla oblongata. Cerebrum was devoid of usual convolutions (elevations), gyri, depressions (grooves), and sulci. The cerebral hemispheres were tightly apposed along a median sulcus called interhemispheric fissure and cerebrum and cerebellum were separated by a small transverse fissure. The olfactory bulb was small structures, and the pineal body was clearly visible. The optic lobes were partially hidden under cerebral hemispheres, but laterally, it was large, prominent rounded or spherical bodies of the midbrain. The hippocampal area appeared as dorso-medial protrusion. Different types of neurons were distinguished in the hippocampus were pyramidal neurons, pyramidal-like neurons, and multipolar neurons, etc. There was rich vascularization in the form of blood capillaries throughout the hippocampus.

Conclusion: Cerebrum was pear shaped and largest part of the brain. Cerebrum hemisphere was smooth devoid of convolutions, gyri, and depressions, but in the surface of cerebellum, there was the presence of a number of transverse depression (grooves) and sulci subdividing into many folds. Olfactory bulb was poorly developed, whereas optic lobes were rounded and large. The exact boundary line of the hippocampus was not discernable. In hippocampus histology, two categories of neuron local circuit neurons and projection neurons, high vascularization and epididymal lining of lateral ventricle were observed. Hippocampal neurons were comparatively larger without any distinct layers. The afferent neurons projected to the medium septum.

Keywords: capillaries, hemisphere, hippocampus, neurons, Vencobb broiler.

Phenotypic and genotypic detection of methicillin-resistant Staphylococcus aureus in hunting dogs in Maiduguri metropolitan, Borno State, Nigeria

Research (Published online: 24-05-2016)

12. Phenotypic and genotypic detection of methicillin-resistant Staphylococcus aureus in hunting dogs in Maiduguri metropolitan, Borno State, Nigeria - Muhammad Mustapha, Yachilla Maryam Bukar-Kolo, Yaqub Ahmed Geidam and Isa Adamu Gulani

Veterinary World, 9(5): 501-506

   doi: 10.14202/vetworld.2016.501-506

Abstract

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Aim: To determine the presence of MRSA in hunting dogs in Maiduguri metropolitan.

Materials and Methods: Phenotypic methods used includes microscopic technique, colony morphology study, catalase-coagulase tests, and the use of mannitol salt agar test, oxacillin resistance screening agar base, and antibiotic susceptibility testing methods. Genotypic approach was used for deoxyribonucleic acid extraction, and the presence of nuc and mecA gene was detected using polymerase chain reaction (PCR) techniques.

Results: Examination of 416 swab samples from nasal and perineal region of dogs revealed a total of 79.5% of S. aureus, where 62.5% of the isolates were MRSA. Molecular analysis revealed that 7nuc genes specific for S. aureus from 20 presumptive MRSA assay were allmecA PCR negative. The isolates were sensitive to gentamicin and ciprofloxacin but proved resistant to cefoxitin and oxacillin.

Conclusion: High isolation rate of MRSA was found in hunting dogs. Significant level (p<0.05) of MRSA was isolated in the nasal cavity of hunting dogs than its perineum. Only nuc genes were detected from the MRSA isolates.

Keywords: dogs, genotypic, methicillin, phenotypic, Staphylococcus aureus.