Effect of pre-partum prilled fat supplementation on feed intake, energy balance and milk production in Murrah buffaloes

Research (Published online: 11-03-2016)

6. Effect of pre-partum prilled fat supplementation on feed intake, energy balance and milk production in Murrah buffaloes - Shikha Sharma, Mahendra Singh, Ashwani Kumar Roy and Sunita Thakur

Veterinary World, 9(3): 256-259

   doi: 10.14202/vetworld.2016.256-259

Abstract

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Aim: To investigate the effect of pre-partum prilled fat feeding on dry matter intake (DMI), energy balance and milk production in Murrah buffaloes.

Materials and Methods: Advance pregnant Murrah buffaloes were either received a dietary supplement of prilled fat at 100 g/day for 35 days pre-partum and at 150 g/day for 95 days post-partum (supplemented group [SG]) or did not receive fat supplement (control group [CG]). DMI and the yields of milk and milk component were measured. A body condition score (BCS) was recorded. Energy balance and gross feed efficiency (GFE) were calculated. DMI and BCS were recorded and milk yield (MY), fat, protein, lactose, solid not fat, energy balance were measured. The fat corrected milk yield was calculated.

Results: The DMI was non-significant between groups and periods of study. BCS of buffaloes improved in the SG than CG (p<0.01). The energy intake in terms of total digestible nutrients (TDN%), TDN intake, digestible energy (DE), metabolizable energy/kg of milk, DE of milk, net energy, and GFE were higher (p<0.01) in SG during post-partum period. Crude protein intake was statistically similar in both the groups. MY was higher (p<0.01) in SG than in CG during 95 days of early lactation. Milk fat, fat corrected MY was higher (p<0.01) in SG however protein, lactose and solid not fat content did not varied between the groups. The feed efficiency of the SG was higher (p<0.01) than the CG during the post-partum period.

Conclusion: It was inferred that prilled fat supplementation augments energy balance and milk production in transition Murrah buffaloes.

Keywords: body condition score, buffaloes, dry matter intake, energy balance, milk yield, prilled fat.

Serum muscle-derived enzymes response during show jumping competition in horse

Research (Published online: 08-03-2016)

5. Serum muscle-derived enzymes response during show jumping competition in horse - Anna Assenza, Simona Marafioti, Fulvio Congiu, Claudia Giannetto, Francesco Fazio, Daniele Bruschetta and Giuseppe Piccione

Veterinary World, 9(3): 251-255

   doi: 10.14202/vetworld.2016.251-255

Abstract

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Aim: The effect of two jumping competitions, performed in two consecutive weekends, on serum creatine phosphokinase (CPK), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH), urea, creatinine (CREA) concentrations were evaluated in 12 healthy jumper horses.

Materials and Methods: Blood sampling was performed before the 1st day of competition (T0), at the end of each show (J1, J2), on the day after the competition (T1); the same sampling plan was followed during the second weekend (J3, J4 and T2).

Results: One-way repeated measures analysis of variance showed an increase in CPK at J1 and J2 respect to T0 and at J3 and J4 respect to all other time points (p<0.05). LDH activity showed an increase at J2 respect to T0, at J3 respect to T0, J1, J2 and at J4 respect to all other time points (p<0.05). AST values increased at J1 and J2 respect to T0 (p<0.05). A significant increase of CREA was found at J3respect to T0, T1 and J1 and at J4 respect to all other time points (p<0.05). A decrease in serum urea levels was found at J1 respect to T0, at J2 and J4 respect to T0 and T1; at T2 respect to T0 (p<0.05). A positive correlation between urea/CPK (p=0.0042, r2=0.030), LDH/CPK (p<0.0001, r2=0.535), CREA/LDH (p<0.0001, r2=0.263), CREA/CPK (p<0.0001, r2=0.496) was observed.

Conclusion: Our results suggest that 5 days recovery period between the two consecutive competition weekends is insufficient to allow muscle recovery and avoid potential additional stress. The findings obtained in this study improve the knowledge about metabolic changes occurring in athlete horse during the competition to identify muscle alterations following show jumping competitions.

Keywords: horse, muscle enzymes, physical exercise, show jumping competition

Effect of dietary supplementation of garlic, ginger and their combination on feed intake, growth performance and economics in commercial broilers

Research (Published online: 08-03-2016)

4. Effect of dietary supplementation of garlic, ginger and their combination on feed intake, growth performance and economics in commercial broilers - V. K. Karangiya, H. H. Savsani, Shrikant Soma Patil, D. D. Garg, K. S. Murthy, N. K. Ribadiya and S. J. Vekariya

Veterinary World, 9(3): 245-250

   doi: 10.14202/vetworld.2016.245-250

Abstract

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Aim: The present study was carried out to evaluate the effect of supplementation of garlic, ginger and their combination in the diets of broiler chickens and assessment in terms of feed intake, growth performance and economics of feeding.

Materials and Methods: A total of 240 1-day-old Cobb-400 broiler chicks were randomly assigned to four dietary treatments each with three replicates of 20 chicks per replicate (n=60). Four experimental diets were formulated in such a way that control diet (T1) contained neither ginger nor garlic. While, birds in group T2 and T3 were fed with diets containing 1% garlic and ginger, respectively. Diet 4 (T4 group) contained a combination of 1% of garlic and ginger. The feeding experiment was carried out for 42 days, and different parameters evaluated includes feed intake, weight gain, feed conversion ratio, gut morphometry, and economics of feeding in terms of return over feed cost (ROFC) and European Performance Efficiency Index.

Results: Feed intake of experimental birds in ginger and mixture of garlic and ginger supplemented groups, i.e., T3 and T4 groups have significantly (p<0.05) higher feed intake as compared to control. While, feeding of garlic have non-significant effect on feed intake as compared to other groups. A body weight gain (g/bird) was found to be significantly (p<0.05) higher in garlic (T2 group) and ginger (T3group) supplemented group as compare to control and garlic and ginger mixture supplemented group (T4 group). Feed conversion ratio was significantly (p<0.05) lower in ginger (T3 group) supplemented group as compare to other groups. Mean villi length, villi width and cryptal depth were significantly (p<0.05) higher in T3 group than rest of all three groups, indicating increased absorptive surface area. ROFC was significantly (p<0.05) lower in T3 and T4 groups as compare to control. However, it was not significantly different between control and T2 group.

Conclusion: On the basis of the results of the study, it is concluded that supplementation of garlic improves the performance of broilers when added at the rate of 1% of broiler ration and can be a viable alternative to antibiotic growth promoter in the feeding of broiler chicken.

Keywords: body weight, broiler chickens, feed intake, garlic, ginger.

Effect of heat stress on reproductive performances of dairy cattle and buffaloes: A review

Review (Published online: 05-03-2016)

3. Effect of heat stress on reproductive performances of dairy cattle and buffaloes: A review - Soumya Dash, A. K. Chakravarty, Avtar Singh, Arpan Upadhyay, Manvendra Singh and Saleem Yousuf

Veterinary World, 9(3): 235-244

   doi: 10.14202/vetworld.2016.235-244

Abstract

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Heat stress has adverse effects on the reproductive performances of dairy cattle and buffaloes. The dairy sector is a more vulnerable to global warming and climate change. The temperature humidity index (THI) is the widely used index to measure the magnitude of heat stress in animals. The bjective of this paper was to assess the decline in performances of reproductive traits such as service period, conception rate and pregnancy rate of dairy cattle and buffaloes with respect to increase in THI. The review stated that service period in cattle is affected by season of calving for which cows calved in summer had the longest service period. The conception rate and pregnancy rate in dairy cattle were found decreased above THI 72 while a significant decline in reproductive performances of buffaloes was observed above threshold THI 75. The non-heat stress zone (HSZ) (October to March) is favorable for optimum reproductive performance, while fertility is depressed in HSZ (April to September) and critical HSZ (CHSZ) (May and June). Heat stress in animals has been associated with reduced fertility through its deleterious impact on oocyte maturation and early embryo development. The management strategies viz., nutrition modification, environment modification and timed artificial insemination protocol are to be strictly operated to ameliorate the adverse effects of heat stress in cattle and buffaloes during CHSZ to improve their fertility. The identification of genes associated with heat tolerance, its incorporation into breeding program and the inclusion of THI covariate effects in selection index should be targeted for genetic evaluation of dairy animals in the hot climate.

Keywords: buffaloes, cattle, heat stress zone, reproductive traits, temperature humidity index.

Peperomia pellucida leaf extract as immunostimulator in controlling motile aeromonad septicemia due to Aeromonas hydrophila in red hybrid tilapia, Oreochromis spp. farming

Research (Published online: 05-03-2016)

2. Peperomia pellucida leaf extract as immunostimulator in controlling motile aeromonad septicemia due to Aeromonas hydrophila in red hybrid tilapia, Oreochromis spp. farming - S. W. Lee, K. Y. Sim, W. Wendy and A. K. Zulhisyam

Veterinary World, 9(3): 231-234

   doi: 10.14202/vetworld.2016.231-234

Abstract

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Aim: This study was revealed the potential of Peperomia pellucida leaf extract as an immunostimulator agent in controlling motile aeromonad septicemia due to Aeromonas hydrophila in red hybrid tilapia, Oreochromis sp.

Materials and Methods: In the present study, minimum inhibitory concentration (MIC) of P. pellucida leaf extract against A. hydrophilawas determined through two-fold microbroth dilution method. The plant extract was screening for its active compound using a gas chromatograph mass spectrometer, and the effectiveness of P. pellucida leaf extract as an immunostimulator agent was evaluated. The experimental fish were fed with medicated feed at three different concentrations (25 mg/kg, PP-25; 50 mg/kg, PP-50; and 100 mg/kg, PP-100) of P. pellucida leaf extract for 1 week before they were intraperitoneally exposed to A. hydrophila. Enzyme-linked immunosorbent assay was carried out to determine the value of antibody response to A. hydrophila in fish from a group of fish that received medicated feed, and the percentage of total cumulative mortality of the experimental fish were observed at the end of the experiment.

Results: The results showed that the major bioactive compound is phytol (40%), and the MIC value was 31.5 mg/L. The value of antibody response to A. hydrophila in fish from a group of fish which received medicated feed (PP-25, 0.128±0.014 optical density [OD]; PP-50, 0.132±0.003 OD; and PP-100, 0.171±0.02 OD) was found significantly higher (p<0.05) compared to fish did not receive medicated feed (0.00 OD). Whereas, percentage cumulative mortality of fish from all groups of fish received medicated feed (PP-25, 18.0±3.2%; PP-50, 18.2±2.8%; and PP-100, 17.7±1.8%) were found significantly lower (p<0.05) compared to a group of fish did not receive medicated feed (83.2±1.4%).

Conclusion: The findings of the present study indicated the huge potential of P. pellucida leaf extract as natural immunostimulator agent for aquaculture uses.

Keywords: Aeromonas hydrophila, immunostimulator, motile aeromonad septicemia, red hybrid tilapia.

Species-specific identification of adulteration in cooked mutton Rista (a Kashmiri Wazwan cuisine product) with beef and buffalo meat through multiplex polymerase chain reaction

Research (Published online: 03-03-2016)

1. Species-specific identification of adulteration in cooked mutton Rista (a Kashmiri Wazwan cuisine product) with beef and buffalo meat through multiplex polymerase chain reaction - M. Mansoor Bhat, Mir Salahuddin, Imtiyaz A. Mantoo, Sheikh Adil, Henna Jalal and M. Ashraf Pal

Veterinary World, 9(2): 226-230

   doi: 10.14202/vetworld.2016.226-230

Abstract

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Aim: Meat adulteration is a serious problem in the meat industry and needs to be tackled to ensure the authenticity of meat products and protect the consumers from being the victims. In view of such likely problem in indigenous meat products of Kashmiri cuisine (Wazwan), the present work was performed to study the detection of beef and buffalo meat in cooked mutton Rista by mitochondrial DNA (mtDNA) based multiplex polymerase chain reaction (PCR) method under laboratory conditions.

Materials and Methods: Three experimental trials were conducted wherein the products were prepared from pure mutton, beef and buffalo meat, and their admixtures in the ratios of 60:20:20, 80:10:10, 90:05:05 and 98:01:01, respectively.

Results: The primers used in the study amplified the cyt b gene fragments of sizes 124 bp, 472 bp and 585 bp for buffalo, cattle and sheep, respectively. It was possible to detect cattle and buffalo meat at the level of 1% in the mixed meat cooked Rista. The multiplex PCR successfully amplified cyt b gene fragments of mtDNA of the target species and thus produced characteristic band pattern for each species. The band intensities of cattle and buffalo in the mixed meat Rista progressively decreased corresponding to their decreasing level from 20% to 1%. Processing, cooking (moist heating) and non-meat formulation ingredients had no effect on detection of meat species adulteration.

Conclusion: The multiplex PCR procedure standardized and developed in this study is simple, efficient, sensitive, reliable and highly specific for detecting falsification of cooked mutton product with beef and buffalo meat up to 1% level.

Keywords: meat adulteration, meat species identification, mitochondrial DNA, multiplex polymerase chain reaction.

Isolation and adaptation of bovine herpes virus Type 1 in embryonated chicken eggs and in Madin–Darby bovine kidney cell line

Research (Published online: 27-02-2016)

20. Isolation and adaptation of bovine herpes virus Type 1 in embryonated chicken eggs and in Madin–Darby bovine kidney cell line -Devprabha Samrath, Sanjay Shakya, Nidhi Rawat, Varsha Rani Gilhare and Fateh Singh

Veterinary World, 9(2): 222-225

   doi: 10.14202/vetworld.2016.222-225

Abstract

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Aim: Objective of the present study was to isolate bovine herpes virus Type 1 (BHV-1) from semen of infected bull and to adapt it onto embryonated eggs and Madin–Darby bovine kidney (MDBK) cell line. Further, the virus was identified by agar gel immunodiffusion (AGID) test.

Materials and Methods: Semen samples were collected from five BHV-1 positive bulls previously confirmed for the presence of antibodies against BHV-1 using avidin-biotin enzyme linked immunosorbent assay test. The virus from semen samples was adapted in chorioallantoic membrane (CAM) of 11-day-old embryonated chickens eggs and in MDBK cell line. The presence of BHV-1 in infected CAM and cell culture fluid was confirmed by AGID test.

Results: Virus infected CAM showed edema, congestion and thickening at first passage level. Small foci ranged from 1 to 2 mm in diameter, scattered all over the membrane were observed at first passage. More severe changes were observed in CAM after serial passaging. The large pock lesions, round in shape with opaque raised edge and depressed gray central area of necrosis ranged from 3 to 5 mm in diameter were developed at fourth passage. Blind passages in MDBK cell culture were made. The MDBK cell line at second passage level showed characteristic cytopathic effect viz. rounding of cells with shrinkage, followed by aggregation or clumping of cells which progressed rapidly and appeared as “bunch of grapes” at 72 h post inoculation. Few cells become elongated when compared with uninfected controls. A homogenate of CAM with distinct pock lesions and infected cell culture fluid developed precipitation line within 48 h against specific anti-BHV-1 immune serum by AGID test.

Conclusion: BHV-1 was easily adapted in CAM of chicken embryos and in MDBK cell line. Virus infected CAM and cell culture fluid showed precipitin band by AGID test.

Keywords: Bovine herpes virus Type 1, chorioallantoic membrane, cytopathic effect, Madin–Darby bovine kidney cell line, pock.