Flow cytometry based profiling of leukocytes: A new method for diagnosis of tropical theileriosis in crossbred cattle

Research (Published online: 11-12-2015)

2. Flow cytometry based profiling of leukocytes: A new method for diagnosis of tropical theileriosis in crossbred cattle - Ramesh B. Jagtap, Amit Gupta and Sushama R. Chaphalkar

Veterinary World, 8(12): 1379-1385

   doi: 10.14202/vetworld.2015.1379-1385

Abstract

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Aim: In India, dairy industries are important for the livelihood of small scale farmers and dairy owners. Tropical theileriosis, mostly affecting dairy cattle and buffaloes is a major threat to dairy and related industries. Tropical theileriosis is caused by Theileria annulata, a hemoprotozoan parasite transmitted by Ixodid ticks of Hyalomma spp. In the present study, we examined the clinical signs, hematological parameters and flow cytometric profile of whole blood in 30 theileriosis affected crossbred cattle. The aim of our study is to analyze, in comparison with clinical and hematological diagnosis, whether flow cytometry based profiling of leukocytes could be used as better, quick and alternative method for diagnosis and screening of bovine tropical theileriosis in crossbred cattle.

Materials and Methods: In this study, we screened parasites in 30 peripheral blood samples from clinical cases of theileriosis by Giemsa’s staining technique in crossbred cattle. Hematological analysis was done to estimate hemoglobin (Hb) content, total red blood cell (RBC) count, total leukocyte count and differential leukocyte count. Further, flow cytometric analysis of whole blood was carried out to study leukocytes profile in affected cattle.

Results: Microscopic examination of stained blood films revealed the presence of piroplasms in erythrocytes and schizonts in lymphocytes. Hematological examination revealed significant (p<0.05) decrease of Hb percent (Hb %), reduced total RBC and total leukocytes, lymphocytosis, eosinopenia, and neutropenia compared to that of apparently healthy cattle. Flow cytometric profiling of leukocytes revealed the severe effect on shape, size, and granularity of leukocytes, marked decrease in granulocytes and 3-5 fold increase in lymphocytes count compared to clinically healthy cattle. Thus, in both methods, namely conventional and flow cytometric analysis, marked lymphocytosis and decrease in other blood cell counts were observed compared to clinically healthy cattle.

Conclusions: From results, it can be concluded that though conventional staining techniques and hematology are efficient in diagnosis of theileriosis, leukocytes profiling based on flow cytometry combined with clinical examination could be a quick, novel and alternative method for diagnosis and screening of clinical tropical theileriosis in crossbred cattle. Thus, there is potential to offer a flow cytometry based diagnostic service for tropical theileriosis in crossbred cattle.

Keywords: clinical examination, flow cytometry, hematology, lymphocytosis, Theileria annulata.

Effect of age and season on the thyroid hormone activity of Mizoram strain female mithun (Bos frontalis)

Research (Published online: 11-12-2015)

1. Effect of age and season on the thyroid hormone activity of Mizoram strain female mithun (Bos frontalis) - Lalsangpuii, M. Ayub Ali, L. Inaotombi Devi, Parthasarathi Behera and Lalsanglura Ralte

Veterinary World, 8(12): 1375-1378

   doi: 10.14202/vetworld.2015.1375-1378

Abstract

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Aim: The aim of the present study was to generate baseline data on the normal values of the thyroidhormone (TH) activity as well as their correlation with age and season.

Materials and Methods: Blood samples (10 ml) were collected from jugular vein of 30 female mithun’s of three different age groupsviz. Calves (6 months to 1 year), heifer (1-3 years) and adult (above 3 years) during the three season’s viz. Monsoon, winter and spring of a year. The serum was analyzed for thyroid stimulating hormone (TSH), triiodothyronine (T3), and thyroxine (T4) activity.

Result: The result showed a significantly (p<0.05) a higher T3 level in heifers followed by adults and calves and higher T4 level in adults followed by heifers and calves in all the seasons. The TSH level was higher in heifers in all the seasons. The winter season recorded higher level of T3, T4, and TSH as compared to the other seasons of a year.

Conclusion: The TSH and T3 level were the highest for aheifer, whereas T4 level was the highest for adults inall the season. Furthermore, the higher level of TH was observed in winter season. The increased level of the TH during the winter season signifies their calorigenic effect. Similarly in heifers, the increased T3 concentrations show its importance in reproductive physiology and its association with ovarian activity. This indicates that age and season have aprofound effect on TH activity of Mizoram strain female mithun.

Keywords: age, mithun, triiodothyronine, thyroxine, thyroid hormone, season.

Comparison of molecular and microscopic technique for detection of Theileria annulata from the field cases of cattle

Research (Published online: 28-11-2015)

17. Comparison of molecular and microscopic technique for detection of Theileria annulata from the field cases of cattle - H. C. Chauhan, B. K. Patel, A. G. Bhagat, M. V. Patel, S. I. Patel, S. H. Raval, H. H. Panchasara, M. D. Shrimali, A. C. Patel and B. S. Chandel

Veterinary World, 8(11): 1370-1374

   doi: 10.14202/vetworld.2015.1370-1374

Abstract

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Aim: Tropical theileriosis is fatal hemoprotozoal disease of dairy animals caused by Theileria annulata. The aim of the present study was to detect the T. annulata and comparison of results of molecular and microscopic techniques.

Materials and Methods: A total of 52 blood samples were collected from the cattle suspected for theileriosis across the Banaskantha district. All the samples were screened for theileriosis using Giemsa’s staining technique and polymerase chain reaction (PCR).

Results: Total of 17 (32.69%) and 24 (46.15%) samples were found positive for theileriosis by microscopic examination and PCR test, respectively. It revealed that the study area is endemic for theileriosis, and the microscopic technique has 70.83% sensitivity and 100% specificity with respect to PCR technique.

Conclusion: It may be concluded from the present study that the PCR is comparatively sensitive technique than microscopic examination and may be recommended to use in the field for screening of theileriosis in the study area, where a high prevalence of diseases have been reported due to intensive dairy farming.

Keywords: Giemsa staining, polymerase chain reaction, theileriosis.

Effect of Moringa oleifera on hematological parameters of calves reared in industrial fluorotic area

Research (Published online: 28-11-2015)

16. Effect of Moringa oleifera on hematological parameters of calves reared in industrial fluorotic area - Kruti Debnath Mandal, M. R. Das, M. Pati, P. D. Pati, A. R. Gupta, R. C. Patra and S. K. Senapati

Veterinary World, 8(11): 1364-1369

   doi: 10.14202/vetworld.2015.1364-1369

Abstract

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Aim: The present study was undertaken to evaluate the ameliorative potential of dried Moringa oleifera fruit powder in fluorosis affected calves reared around the vicinity of aluminium smelter plant.

Materials and Methods: Total 107 calves were screened on the basis of clinical signs and higher plasma fluoride (more than 0.2 ppm) level for evidence of fluorosis. Out of that, 90 samples found positive and from them 18 calves of 6-12 months age group were selected and divided equally into three groups named as Group II, III, and IV. Group II remained as disease control group whereas Group III calves were supplemented with dried M. oleifera fruit powder of 25 g/calve for 60 days. Group IV calves were supplemented with calcium carbonate at 100 mg/kg body weight and boric acid at 10 mg/kg for the same experimental period. Group I consisted of six numbers of healthy calves taken from the non-fluorotic zone, i.e. Bhubaneswar. Plasma fluoride level, hemoglobin (Hb), packed cell volume (PCV), total leukocyte count (TLC), differential count (DC), total erythrocyte count, mean corpuscular volume (MCV), mean corpuscular Hb (MCH), and MCH concentration (MCHC) were estimated on day 0, 30, and 60 of the experiment.

Results: Supplementation of dried M. oleifera fruit powder to fluorosis affected calves resulted in significant reduction in plasma fluoride level and increase in Hb%, PCV, TLC and altered DC. Similar results were also recorded in calcium+boron group, except PCV and Hb. No significant changes were observed in MCV, MCH, and MCHC values.

Conclusion: The present study concluded that supplementation of dried M. oleifera fruit powder daily for 60 days has shown protection against chronic fluoride toxicity in calves.

Keywords: aluminum smelter, boron, calcium, calves, fluorosis, Moringa oleifera.

Genetic and non-genetic factors affecting morphometry of Sirohi goats

Research (Published online: 28-11-2015)

15. Genetic and non-genetic factors affecting morphometry of Sirohi goats - S. D. Dudhe, S. B. S. Yadav, R. K. Nagda, Urmila Pannu and

G. C. Gahlot

Veterinary World, 8(11): 1356-1363

   doi: 10.14202/vetworld.2015.1356-1363

Abstract

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Aim: The aim was to estimate genetic and non-genetic factors affecting morphometric traits of Sirohi goats under field condition.

Materials and Methods: The detailed information of all animals on body measurements at birth, 3, 6, 9, and 12 months of age was collected from farmer’s flock under field condition born during 2007-2013 to analyze the effect of genetic and non-genetic factors. The least squares maximum likelihood program was used to estimate genetic and non-genetic parameters affecting morphometric traits.

Results and Discussion: Effect of sire, cluster, year of birth, and sex was found to be highly significant (p<0.01) on all three morphometric traits, parity was highly significant (p<0.01) for body height (BH) and body girth (BG) at birth. The h² estimates for morphometric traits ranged among 0.528±0.163 to 0.709±0.144 for BH, 0.408±0.159 to 0.605±0.192 for body length (BL), and 0.503±0.197 to 0.695±0.161 for BG.

Conclusion: The effect of sire was highly significant (p<0.01) and also h² estimate of all morphometric traits were medium to high; therefore, it could be concluded on the basis of present findings that animals with higher body measurements at initial phases of growth will perform better with respect to even body weight traits at later stages of growth.

Keywords: correlation, genetic, morphometric traits, non-genetic and Sirohi.

The effects of protein dietary supplementation on fecal egg counts and hematological parameters in goat kids with subclinical nematodosis

Research (Published online: 24-11-2015)

14. The effects of protein dietary supplementation on fecal egg counts and hematological parameters in goat kids with subclinical nematodosis - Priyanka Konwar, S. P. Tiwari, M. Gohain and Kiran Kumari

Veterinary World, 8(11): 1351-1355

   doi: 10.14202/vetworld.2015.1351-1355

Abstract

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Aim: The aim of the present study was to assess the effect of dietary supplementation with different levels of protein on fecal egg counts and hematological parameters in goat kids with subclinical nematodosis under semi-intensive condition.

Materials and Methods: 20 goat kids (3-5 months old with an average body weight of 8.90 kg) were randomly allocated to four groups: T1, served as a negative control, without receiving concentrate feed, and T2, T3, and T4 that received concentrate feed containing 16, 20, and 24% digestible crude protein, respectively. The experiment was carried out for 60 days.

Results: In this study, protein supplementation had a significant (p<0.05) effect on fecal egg counts even after 15 days; hemoglobin (Hb) (g/dl) after 45 days; total leukocyte count (103/mm3) and total erythrocyte count (106/mm3) after 30 days; packed cell volume (%), lymphocyte (%), and eosinophil (%) after 15 days of supplementation, whereas monocyte (%) and neutrophil (%) values were not significantly influenced by protein supplementation effect during the entire experiment. The values of mean corpuscular volume (fl) were affected significantly (p<0.05, p<0.01) due to protein supplementation after 30 days, mean corpuscular Hb (MCH) (pg) after 45 days, but MCH concentration (g/dl) was not significantly different among the experimental groups during the entire experiment.

Conclusion: The dietary supplementation with different levels of protein significantly improved the hematological profiles and inhibited the nematodosis infection in the experimental goat kids.

Keywords: fecal egg count, goat kids, hematological parameter, nematodosis, protein dietary supplementation.

Clinicopathological evaluation of non-parasitic dermatoses in canines

Research (Published online: 24-11-2015)

13. Clinicopathological evaluation of non-parasitic dermatoses in canines - M. J. Sindha, B. J. Trangadia, P. D. Vihol, R. S. Parmar and B. V. Patel

Veterinary World, 8(11): 1346-1350

   doi: 10.14202/vetworld.2015.1346-1350

Abstract

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Aim: The present study has been carried out to detect non-parasitic dermatoses in canines brought at the Nandini Veterinary Hospital, Surat.
Materials and Methods: The current investigation was carried out on skin scrapping, skin biopsy specimens, blood, and serum samples of 210 freshly registered cases of dogs with dermatological afflictions. Dogs found healthy on clinical examination were used as control animals (n=15). The incidence of non-parasitic dermatoses has been recorded as per age, breed, and sex of dogs. For bacterial isolation, the pus/exudates samples were collected from 40 cases of pyoderma and streaked onto brain-heart infusion agar while 13 skin scrapping samples were inoculated on Sabouraud’s dextrose agar with chloramphenicol for isolation of fungi. The organisms were identified on the basis of gross and microscopic observation of cultural growth on media. The blood and sera samples were also collected to note alteration in hematology and biochemical parameters, respectively. Tissue samples from lesions were collected and subsequently preserved in 10% neutral buffered formalin for histopathology.
Results: Out of 210 cases of dermatoses, 60 cases were of non-parasitic dermatoses, i.e., 28.57%. Of these, bacterial skin infections (pyoderma) were found to be the predominant at 80.00%, followed by other non-parasitic dermatological disorders, i.e., 11.67% and fungal skin infection, i.e., 8.33%. The dogs belonging to age group 1-3 years showed greater susceptibility to non-parasitic dermatological conditions. Breed wise incidence of pyoderma was found more in the Pomeranian breed (20.83%), whereas fungal skin affections were found to be higher in mongrel breed (60.00% and 42.86%, respectively). Male dogs showed greater involvement in bacterial, fungal, and other non-parasitic dermatoses. Bacteriological culture examination of 40 pus swabs resulted in the growth of 39 bacterial isolates. Mycological culture of skin scrapings from 13 suspected cases of fungal dermatoses resulted in the recovery of five fungal isolates. Hematological and serum biochemical parameters revealed a significant difference in all cases of non-parasitic dermatoses. Histopathological study revealed characteristic changes like infiltration of neutrophils with perifolliculitis, hyperkeratosis, and rafts of acantholytic cells. Histochemical staining revealed purple or magenta color fungal elements.

Conclusion: Based on current experiment it has been concluded that among non-parasitic dermatoses bacterial and fungal skin infections are the main ailments, followed by nutritional and other causes in adult and male dogs which can be diagnosed by cultural inoculation, microscopic examination of skin scrapings, and dermatohistopathology along with hematology and biochemistry.

Keywords: bacterial, canines, fungal, non-parasitic dermatoses.