Occurrence of multidrug-resistant Salmonella in cattle carcass and contact surfaces in Kwata slaughterhouse, Awka, Anambra State, Nigeria

Research (Published online: 12-05-2020)

9. Occurrence of multidrug-resistant Salmonella in cattle carcass and contact surfaces in Kwata slaughterhouse, Awka, Anambra State, Nigeria

Uju Catherine Okafor, Simeon Chibuko Okafor and Akwoba Joseph Ogugua

International Journal of One Health, 6(1): 49-55

ABSTRACT

Background and Aim: Contamination of meat with Salmonella could result in food-borne disease outbreaks among the consumers. However, there is a dearth of data assessing the presence of Salmonella in beef in Anambra State. Therefore, this study determined the prevalence and antimicrobial susceptibility of Salmonella in beef and slaughter/processing facilities in Kwata slaughterhouse.

Materials and Methods: Swab samples (200) randomly collected were cultured for the isolation of Salmonella and the isolates subjected to antimicrobial susceptibility test. Data obtained were analyzed using t-test and analysis of variance with p< 0.05 considered statistically significant.

Results: Of the 200 samples cultured, 33.5% (67/200) yielded Salmonella isolates. The mean Salmonella load (colony-forming unit [CFU]/cm2) for different contact surfaces (before and after contact with carcasses) was as follows: Slaughter floor, 1.1×1010±1.1×106a and 1.0×1010±1.1×106b; display table, 1.1×1010±11.1×106a and 1.0×1010±1.1×106b; washing bucket 1.01×1010±1.0×106a and 0.8×1010±0.1×106b; knife, 1.1×1010±1.10×106a and 1×1010±1.0×106b; boot, 1.1×1010±1.0×106a and 1.0×1010±1.10×106b; file, 1.1×1010±1.0×106a and 1.0×1010±0.1×106b; and wheelbarrow, 1.1×1010±1.0×106a and 1.01×1010±0.11×106b. Salmonella counts decreased significantly (p<0.05) in the presented order from slaughter floor to wheelbarrow after contact with carcasses. On the other hand, there was a significant (p<0.05) increase in washing water Salmonella counts before and after (0.7×1010±0.10×106a and 1.0×1010±1.0×106b CFU/100 ml) carcasses wash. To each of the antimicrobials tested, the percentage of the 67 isolates found resistant was as follows: ciprofloxacin, 25.4%; ofloxacin, 27%; ceftriaxone, 35.8%; amoxicillin/clavulanic acid, 88.1%; chloramphenicol, 59.7%; gentamicin, 34.3%; streptomycin, 49.3%; nalidixic acid, 49.3%; trimethoprim/sulfamethoxazole, 76%; nitrofurantoin, 89.6%; and ampicillin, 100%.

Conclusion: Antimicrobial-resistant Salmonella were isolated from beef and slaughter/processing facilities in Anambra State. This underscores the need for a coordinated one health approach for the improvement of hygienic standard during slaughter/processing in the slaughterhouse surveyed, to limit meat contamination and hence safeguard human health.

Keywords: beef, contact surfaces, Kwata slaughterhouse, resistance, Salmonella.

Profile of follicle-stimulating hormone and polymorphism of follicle-stimulating hormone receptor in Madrasin cattle with ovarian hypofunction

Research (Published online: 11-05-2020)

7. Profile of follicle-stimulating hormone and polymorphism of follicle-stimulating hormone receptor in Madrasin cattle with ovarian hypofunction

Budi Utomo, Emmanuel Djoko Putranto and Amaq Fadholly

Veterinary World, 13(5): 879-883

ABSTRACT

Background and Aim: The follicle-stimulating hormone (FSH) gene is an essential regulator of fertility in livestock. This study aims to provide information on the genetic makeup of Madrasin cattle experiencing hypofunction by the FSH profile and FSH receptors (FSHR) polymorphism.

Materials and Methods: Blood samples were collected from the Bangkalan regency in Indonesia. DNA was isolated and purified following the extraction protocol of polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism.

Results: Our results showed that the FSH gene had a band length of 310 bp and produce two alleles (A and B) with restriction enzymes at 250 bp, 230 bp, and 145 bp. Furthermore, the FSHR gene had a band length of 303 bp and produced two homozygous genotypes: GG at bp 239 and CC at bp 188.

Conclusion: Based on these differences, there was no change in allele frequency and genotype between Madura and Madrasin cattle due to crossbreeding with Limousin cattle. Thus, further detailed investigations of Madrasin cattle are required to elucidate the profile of the LH and LHR genes.

Keywords: follicle-stimulating hormone, follicle-stimulating hormone receptors, hypofunction, Madrasin.

Accuracy of methods for diagnosing heart diseases in cats

Research (Published online: 09-05-2020)

6. Accuracy of methods for diagnosing heart diseases in cats

Tanarut Laudhittirut, Natrada Rujivipat, Kornnicha Saringkarisate, Peeraya Soponpattana, Teerawat Tunwichai and Sirilak Disatian Surachetpong

Veterinary World, 13(5): 872-878

ABSTRACT

Aim: This study aimed to determine the accuracy of the current methods for diagnosing heart diseases in cats.

Materials and Methods: The data of 58 cats were retrospectively retrieved. Cats were classified into two groups: Thirty-eight cats with heart diseases and 20 healthy cats. Echocardiography was the gold standard method for diagnosing heart disease. The results of seven methods were retrieved: (1) Vertebral heart score (VHS) with a cutoff value >8, (2) VHS with a cutoff value >8.5, (3) multiplication of cardiac length (L) and width (W), (4) multiplication of cardiac L and W divided by the L of the fourth sternal thoracic bone, (5) N-terminal Pro-B-type natriuretic peptide (NT-proBNP) point-of-care test, (6) subjective ultrasonographic assessment of the left atrial size, and (7) subjective radiographic assessment of the left atrial size. Cross-tabulation was used to calculate the sensitivity, specificity, accuracy, positive predictive value, and negative predictive value for each test. This study found that using the NT-proBNP point-of-care test was optimal in the diagnosis of cats with heart disease.

Results: The subjective ultrasonographic assessment of the left atrial size was good for diagnosing hypertrophic cardiomyopathy and congestive heart failure.

Conclusion: This study showed that the more tests used, the higher the reliability of the diagnosis.

Keywords: cardiac disease diagnosis, N-terminal Pro-B-type natriuretic peptide, thoracic radiography, ultrasonography.

Celery (Apium graveolens) as a potential antibacterial agent and its effect on cytokeratin-17 and other healing promoters in skin wounds infected with methicillin-resistant Staphylococcus aureus

Research (Published online: 09-05-2020)

5. Celery (Apium graveolens) as a potential antibacterial agent and its effect on cytokeratin-17 and other healing promoters in skin wounds infected with methicillin-resistant Staphylococcus aureus

Yos Adi Prakoso, Chylen Setiyo Rini, Asih Rahayu, Miarsono Sigit and Dyah Widhowati

Veterinary World, 13(5): 865-871

ABSTRACT

Background and Aim: Antimicrobial resistance is a global problem caused by extensive utilization of antibiotics that promote gene resistant among bacteria, including Staphylococcus aureus. This study aimed to analyze the potential effects of celery (Apium graveolens) extract as an antioxidant and antimicrobial agent against methicillin-resistant S. aureus (MRSA), in vitro and in vivo.

Materials and Methods: Celery was extracted and tested against a MRSA isolate in vitro. The minimum inhibitory concentration (MIC) against MRSA for the celery extract (CE) was determined to be 0.1% and it was formulated into a cream. A total of 30 female Sprague Dawley rats were divided into five groups: Group 1, negative control; Group 2, positive control; Group 3, treated with 0.05% CE cream; Group 4, 0.1% CE cream; and Group 5, 0.2% CE cream. All animals in the groups were exposed to a full-thickness skin biopsy on the dorsal portion, and they were infected with 30 μL of 105 colony-forming units of the MRSA isolate. The treatment was administered twice a day for 7 days. The skin samples were collected on days 3 and 7 after the treatment. The skin tissue was examined histologically using hematoxylin and eosin, Gram staining, and immunohistochemistry against cytokeratin (CK)-17.

Results: Results showed that 0.2% of CE cream was the best treatment for wounds infected with MRSA. CE (0.2%) cream increased skin reepithelialization, fibroblast proliferation, and CK-17 expression; it also decreased the percentage of wound area, inflammatory cell infiltration, and bacterial colonization in skin wound tissue compared to the other treatments (p≤0.05).

Conclusion: This study demonstrated that celery could be utilized as an alternative herbal therapy against MRSA-associated skin infections.

Keywords: antimicrobial, celery, cytokeratin-17, methicillin-resistant Staphylococcus aureus, wound healing.

Ophthalmic findings in sheep treated with closantel in Curitiba, Brazil

Research (Published online: 08-05-2020)

4. Ophthalmic findings in sheep treated with closantel in Curitiba, Brazil

Marianna Bacellar-Galdino, Fabiano Montiani-Ferreira, Andre Tavares Somma, Ricardo Guilherme D'Otaviano de Castro Vilani and Ivan Roque de Barros Filho

Veterinary World, 13(5): 860-864

Background and Aim: Closantel is a widely used anti-parasitic drug that is known to cause ophthalmic problems that lead to blindness. The aim of this study was to investigate the possible electroretinographic changes in sheep that received closantel.

Materials and Methods: Twenty-four 30-day-old Suffolk sheep were split into control group (12 animals) and closantel group (12 animals). The latter group received 15 mg/kg of closantel subcutaneously immediately after the first electroretinography (ERG). The ISCEV protocol was used to perform the ERGs pre-dose (0), 7, and 30 days after treatment. Statistical analyses to compare ERG responses using t-test and analysis of variance were performed (p<0.05). Three months later, the animals were euthanized and the eyes and a part of optic nerve were collected for histopathology. Photography of the retina and optic nerve was taken, and measures of the retinal layers were made and analyzed by paired t-test.

Results: Closantel group showed a significant increase of the mean scotopic a-wave amplitude from 0 to 7 days after closantel administration, using a stimulus of 10,000 mcd.s/m2 and a decrease of the mean scotopic and photopic a-wave amplitude (from 7 to 30 days) using the same flash intensity, as well as a decrease in mean photopic b-wave amplitude (from 7 to 30 days) within the group. Control group showed a significant increase of the mean scotopic b-wave implicit time from pre to 30 days after treatment and an increase of the mean scotopic a-wave implicit time from pre to 7 days after treatment, with the stimulus of 10,000 mcd.s/m2. This group also showed a decrease in mean photopic b-wave implicit time (from pre to 30 days after treatment), using a stimulus of 10,000 mcd.s/m2 and a decrease in mean photopic a-wave implicit time from pre to 30 days after treatment, using a stimulus of 3000 mcd.s/m2. The no difference was found in images neither in the measurements of the retina layers.

Conclusion: As observed by ERG responses and the histopathology, a dose of 15 mg/kg of closantel does not significantly affect retinal and optic nerve structures in sheep but the electroretinographic results, however, showed alterations on the phototransduction.

Keywords: closantel electroretinography, histopathology, sheep.

The different hormonal system during exercise stress coping in horses

Review (Published online: 06-05-2020)

3. The different hormonal system during exercise stress coping in horses

Adriana Ferlazzo, Cristina Cravana, Esterina Fazio and Pietro Medica

Veterinary World, 13(5): 847-859

ABSTRACT

The review discusses the hormonal changes during exercise stress. The exercise generally produces a rise of adrenaline (A), noradrenaline (NA), adrenocorticotropic hormone (ACTH), cortisol, glucagon, growth hormone, arginine vasopressine, etc., and a drop of insulin. The hormonal events during reestablishment of homeostasis due to exercise stress can be divided into a catabolic phase, with decreased tolerance of effort, and reversible biochemical, hormonal and immunological changes, and an anabolic phase, with a higher adaptive capacity, and enhanced performance. The two main hormonal axes activated in the catabolic phase are sympathetic–adrenal–medullary system and hypothalamic-pituitary-adrenal (HPA) axis, while in the anabolic phase, growth hormone-insulin-like factor I axis, and gonadal axes. The hormonal responses during exercise and recovery can be regarded as regulatory and integrated endocrine responses. The increase of catecholamines and ACTH is dependent on the intensity of exercise; a marked increase in plasma A occurs during exercises with high emotional content. The response of cortisol is correlated with the duration of exercise, while the effect of exercise duration on β-endorphin changes is highly dependent on the type of exercise performed. Cortisol and β-endorphin changes usually occur in phase, but not during exercises with high emotional content. Glucocorticoids and iodothyronines are involved in meeting immediate energy demands, and a model of functional interactions between HPA axis and hypothalamic-pituitary-thyroid axis during exercise stress is proposed. A modulation of coping responses to different energy demanding physical activities required for sport activities could be hypothesized. This review supports the proposed regulation of hypophysiotropic TRHergic neurons as metabolic integrators during exercise stress. Many hormonal systems (ghrelin, leptin, glucose, insulin, and cortisol) are activated to control substrate mobilizations and utilization. The cardiovascular homeostasis, the fluid and electrolyte balance during exercise are highly dependent on vasoactive hormones (antidiuretic hormone, atrial natriuretic peptide, renin–angiotensin–aldosterone, and prostaglandins) control.

Keywords: equines, hormones, hypothalamic-pituitary-adrenal axis, iodothyronines, physical exercise.

Individual variation in fresh and frozen semen of Bali bulls (Bos sondaicus)

Research (Published online: 05-05-2020)

2. Individual variation in fresh and frozen semen of Bali bulls (Bos sondaicus)

R. Indriastuti, M. F. Ulum, R. I. Arifiantini and B. Purwantara

Veterinary World, 13(5): 840-846

ABSTRACT

Aim: This study aimed to analyze the individual factors influencing the sperm quality of Bali bulls at Baturiti Artificial Insemination (AI) center.

Materials and Methods: Semen that was ejaculated from nine Bali bulls was collected using artificial vaginas (n=5/bull). Semen ejaculates were evaluated immediately after collection to measure the quality of the fresh semen, including semen volume, sperm concentration, progressive motility, membrane integrity (MI), and abnormal morphology. Frozen semen was evaluated for progressive sperm motility, concentration, viability, MI, abnormal morphology, and deoxyribonucleic acid (DNA) fragmentation. Other secondary data, focusing on semen quantity (semen volume and sperm concentration), were also collected from frozen the semen production data of the Baturiti AI center from 2017 to 2019. Data were analyzed statistically using a completely randomized design, and one-way analysis of variance was applied to find differences among individual bulls.

Results: Significant differences (p<0.05) were found among the bulls in semen volume, sperm motility, concentration, and MI of the fresh semen. Significant differences (p<0.05) were also found among the bulls in sperm motility, viability, MI, abnormal morphology, and DNA fragmentation of the frozen semen.

Conclusion: Individual variation in all the tested sperm parameters of the fresh semen of Bali bulls, except sperm viability and abnormalities, was noted. Similarly, individual variation in all the tested sperm parameters in frozen semen, except sperm concentration, was noted. Therefore, individual factors can be used for selecting a superior bull in Bali cattle.

Keywords: Bali bull, individual factors, sperm quality.

Effects of body condition score and estrus phase on blood metabolites and steroid hormones in Saanen goats in the tropics

Research (Published online: 04-05-2020)

1. Effects of body condition score and estrus phase on blood metabolites and steroid hormones in Saanen goats in the tropics

Pradita Iustitia Sitaresmi, Budi Prasetyo Widyobroto, Sigit Bintara and Diah Tri Widayati

Veterinary World, 13(5): 833-839

ABSTRACT

Background and Aim: Body condition scores (BCSs) assessment can be a potential management tool for the improvement of animal performance. The study investigated whether blood metabolic (protein, glucose, blood urea nitrogen, and cholesterol) and steroid hormonal (progesterone, estrogen, and cortisol) parameters are affected by the BCS and estrous status in Saanen goat.

Materials and Methods: Blood samples were collected from three groups of mature, dry, and non-pregnant female goats with different BCSs: 2-2.9 (BCS 2), 3-3.9 (BCS 3), and 4-4.9 (BCS 4) on a BCS scale ranged from 1 to 5. Blood serum was collected (n = 160) to determine the blood metabolic profile and steroid hormone levels, depending on the follicular status.

Results: The results demonstrate a variation in the serum metabolite and hormone (glucose, BUN, protein, estrogen, and cortisol) levels at different BCSs and at different phases of the estrous cycle. The hormonal profiles were significantly different (p<0.05) between the estrous cycle phases. The results suggest that BCSs were associated with blood metabolic profiles and steroid hormone levels.

Conclusion: As it can be concluded, an association between steroid hormone levels and the phases of the estrous cycle existed in Saanen goats.

Keywords: blood serum, body condition score, estrus phase, hormones, Saanen goat.

Microbial risk assessment of ready-to-eat fast foods from different street-vended restaurants

Research (Published online: 03-05-2020)

8. Microbial risk assessment of ready-to-eat fast foods from different street-vended restaurants

Abdullah Al Momen Sabuj, Zobayda Farzana Haque, Md. Iftekhar Younus, Amrita Pondit, Nanda Barua, Md. Golzar Hossain, Md. Alimul Islam and Sukumar Saha

International Journal of One Health, 6(1): 41-48

ABSTRACT

Aim: Microbiological risk analysis of ready-to-eat fast foods for sale on the campus of Bangladesh Agricultural University was undertaken to understand the contribution of such foods to foodborne disease.

Materials and Methods: From each of 10 randomly selected fast food outlets, a total of 120 samples of six food items were collected to assess common microbial load. In parallel, vendors were asked about their food production and selling practices, while consumers (n=200) were asked about their consumption of fast foods and whether they had experienced symptoms of foodborne disease.

Results: Aerobic plate count (APC) varied from 6.92 to 7.24 log colony-forming unit (cfu)/g, Staphylococcus spp. 4.67 to 5.15 log cfu/g, Salmonella spp. 3.67 to 4.22 log cfu/g, and Escherichia coli 4.10 to 4.58 log cfu/g. Microbial risk assessment of Staphylococcus spp., Salmonella spp., and E. coli for daily, weekly, or monthly consumption was estimated by Monte Carlo simulation (10,000 iterations). The consumer survey showed 57% chance of being infected by ready-to-eat fast food samples. The calculated mean annual risks of Staphylococcus spp., Salmonella spp., and E. coli infection were about 100% in all cases.

Conclusion: Thus, the study revealed high risk of infection associated with the consumption of ready-to-eat fast foods.

Keywords: fast foods, microbial quality, microbial risk, Monte Carlo simulation.

Histomorphological differentiation of the skin of leopard (Panthera pardus), leopard cat (Prionailurus bengalensis), Bengal tiger (Panthera tigris), and golden jackal (Canis aureus)

Research (Published online: 30-04-2020)

32. Histomorphological differentiation of the skin of leopard (Panthera pardus), leopard cat (Prionailurus bengalensis), Bengal tiger (Panthera tigris), and golden jackal (Canis aureus)

Chukkath Vijayan Rajani, Harshad Sudhir Patki, Patgiri Simanta, Kalaripparambath Surjith, Padinjare Melepat Deepa and Mampillikalam Pradeep

Veterinary World, 13(4): 827-832

ABSTRACT

Background and Aim: Leopard (Panthera pardus), leopard cat (Prionailurus bengalensis) Bengal tiger (Panthera tigris), and golden jackal (Canis aureus) are carnivores. Leopard and Bengal tiger are listed in the red list as vulnerable species by the International Union for Conservation of Nature and Natural resources. Leopard cat and golden jackal are grouped under animals of least concern. A wide variation exists in the structure of the skin and pattern of hair follicles among domestic and wild mammals. Thus, the study aims to create a baseline data on the skin of leopard, leopard cat, Bengal tiger, and golden jackal and the data so obtained may form an indispensable tool in wildlife forensics.

Materials and Methods: Skin samples of leopard (n=3), leopard cat (n=4), Bengal tiger (n=3), and golden jackal (n=4) were collected from the Department of Pathology, College of Veterinary and Animal Sciences, Pookode. The samples were processed for paraffin embedding. Horizontal and vertical sections of 5 μm thickness were used for histological staining techniques. Observations on the layers and features of epidermis, hair follicle pattern and glands, namely, sweat and sebaceous were recorded.

Results: Skin comprised an outer epidermis and an inner dermis. Keratinized stratified squamous epithelium made up the epidermis. Stratum basale, stratum spinosum, stratum granulosum, and stratum corneum were discernible in leopard, Bengal tiger, and golden jackal. In leopard cat, stratum basale, stratum spinosum, and stratum corneum were present. Compound hair follicles were a characteristic feature of all species. However, the pattern varied. In leopard, leopard cat and Bengal tiger, a single large primary guard hair was encircled by compound follicles. The number of surrounding compound follicles ranged between five to seven in leopard, two to five in leopard cat, and three to seven in Bengal tiger. Each compound follicle, in turn contained, one to two coarse primary hair follicles and several fine secondary hair follicles. Compound follicles arranged as clusters of three were a salient attribute in jackal. The central follicle was comparatively larger than the lateral ones. Each compound follicle comprised a single long, primary hair, and six to eight smaller secondary hairs.

Conclusion: Histological variation in the skin of the leopard, leopard cat, Bengal tiger, and golden jackal was established. The data form a valuable basis for comparative histology of wild carnivores. Further, the data may be of value in the identification of the unknown skin samples of wild carnivores.

Keywords: Bengal tiger, golden jackal, leopard cat, leopard, skin.

Intestinal cestodes of chicken are effectively killed by quinoline-rich extract of Spilanthes acmella

Research (Published online: 30-04-2020)

31. Intestinal cestodes of chicken are effectively killed by quinoline-rich extract of Spilanthes acmella

Pawi Bawitlung Lalthanpuii and Kholhring Lalchhandama

Veterinary World, 13(4): 821-826

ABSTRACT

Background and Aim: Spilanthes acmella is used for the treatment of intestinal helminth infections in Mizo traditional medicine. In spite of a variety of drugs developed for helminthiases, an entirely safe and absolutely effective drug is still lacking, so much so that infections remain a major problem in human and animal welfare. In this study, we attempted to substantiate S. acmella as an anticestodal agent.

Materials and Methods: The aqueous extract of the aerial parts of S. acmella was prepared and from it a bioactive fraction was obtained using column chromatography. Chemical analyses were done using thin-layer chromatography (TLC) and gas chromatography–mass spectrometry (GC–MS). Helminth survival test was performed in vitro on an intestinal cestode, Raillietina tetragona. Structural effects on the cestode were examined under scanning electron microscopy.

Results: From the bioactive fraction of S. acmella extract, TLC indicated the presence of an aromatic quinone, which was identified using GC–MS as a quinoline derivative (2,2,4-trimethyl-1,2-dihydroquinoline having a retention time of 24.97 min and chemical formula of C12H15N). The quinoline-rich fraction showed concentration-dependent activity against R. tetragona as that of albendazole. Scanning electron microscopy of the treated cestode revealed classic anthelmintic effects such as tegumental shrinkage and damage of surface organs. The scolex was shrunk, suckers were degenerated with disintegrated spines, and rostellum was completely collapsed. There were severe damages on the tegument and formation of pit-like scars on the proglottids.

Conclusion: The efficacy of S. acmella extract and structural damages it caused on the cestode indicates that it is a potential source of anthelmintic agent and that 2,2,4-trimethyl-1,2-dihydroquinoline contributes to its antiparasitic activity.

Keywords: cestode, helminthiasis, quinoline, Spilanthes acmella, tegument.

Efficacy and safety of ethanolic Curcuma longa extract as a treatment for sand tampan ticks in a rabbit model

Research (Published online: 29-04-2020)

30. Efficacy and safety of ethanolic Curcuma longa extract as a treatment for sand tampan ticks in a rabbit model

Sobhy Abdel-Shafy, Abdullah D. Alanazi, Hanan S. M. Gabr, Ahmad M. Allam, Hala A. A. Abou-Zeina, Ragab A. Masoud, Doaa E. Soliman and Mohammad Yahya Alshahrani

Veterinary World, 13(4): 812-820

Background and Aim: The soft tick Ornithodoros savignyi is distributed throughout Africa, including Egypt. It primarily attacks camels, cattle, donkeys, and cows; and rarely affects humans. This study evaluated the acaricidal efficacy of ethanolic Curcuma longa extract (Turmeric) on the second nymphs of O. savignyi and then investigated the safety of this herb in rabbits.

Materials and Methods: The nymphs were immersed in 10, 5, 2.5, 1.25, and 0.625 mg/ml ethanolic C. longa extract. An additional group was immersed in ethanol as a control. On the 1st, 7th, and 15th-day post-treatment, the mortality percentages, LC50 and LC95 were calculated. The ticks exposed to 10 mg/ml ethanol C. longa extract were investigated by scanning electron microscopy (SEM). Three male New Zealand White rabbits were orally administered 2 ml (two doses) of 10 mg/ml ethanolic C. longa extract, and another three rabbits were orally given two doses of 2 ml of absolute ethanol as a negative control. Histopathological examination of the kidney and liver hematology and the kidney and liver function was performed. Chemical analysis of the extract was determined by gas chromatography-mass spectrometry (GC-MS).

Results: The LC50 and LC95 were 1.31 and 15.07, 1.07 and 8.56, and 0.81 and 6.97 mg/ml on the 1st, 7th, and 15th day, respectively. SEM revealed that mamillae and spots on the surfaces of the treated ticks were not discriminating except for some clefts on the surfaces. The histological examination, blood profile, and biochemical analyses revealed no significant differences between the treated and untreated rabbits (p>0.05). GC/MS analysis revealed 50 compounds, and curcumene and t umerone were found to be the major constituents of this ethanolic extract.

Conclusion: The ethanolic C. longa extract produced a strong acaricidal effect on the second nymph of O. savignyi, and it was safe to use in rabbits.

Keywords: histopathology, scanning electron microscopy, soft ticks, tick control, turmeric.

Normal reference ranges of ocular physiology and sonographic biometry of Latvian Hunting dogs

Research (Published online: 28-04-2020)

29. Normal reference ranges of ocular physiology and sonographic biometry of Latvian Hunting dogs

Līga Kovaļčuka and Gundega Mūrniece

Veterinary World, 13(4): 807-811

ABSTRACT

Background and Aim: The Latvian Hunting dog is the only national dog breed in Latvia and so far, there are no data on the important ophthalmological data pertaining to clinical tests and ocular biometry. The aim of this study was to improve the ocular examination and to document normal reference ranges of the globe axial length (AXL), anterior chamber depth (ACD), lens thickness (LT), intraocular pressure (IOP), and Schirmer tear test (STT).

Materials and Methods: Sixteen Latvian Hunting dogs (32 eyes) of varying age and sex were included in this study. All dogs underwent an ophthalmic examination followed by tear production (STT) and IOP (TonoVet®) measurements. Biometric examination using A-mode ultrasonography was done under topical corneal anesthesia using 0.5% proparacaine hydrochloride. The eyes were scanned using A-mode ultrasonography with a 10 MHz transducer by transcorneal approach. The reference values of IOP, STT, AXL, ACD, and LT are expressed as mean ± standard deviation (SD).

Results: Statistical analysis using the SPSS v.23 and Microsoft Excel 2013 showed no statistically significant differences between the right and left eyes for either of the tests. The mean±SD value of STT and IOP values was 20.9±3.2 mm/min and 17.7±3.2 mmHg, respectively. The mean±SD of the AXL, ACD, and LT obtained by A-mode ultrasound was 2.21±0.1 cm, 0.35±0.1 cm, and 0.42±0.06 cm, respectively.

Conclusion: This study documents the normal reference ranges of important ophthalmological clinical tests (IOP and STT) and ocular biometry (AXL, ACD, and LT) in Latvian Hunting dogs.

Keywords: anterior chamber depth, axial length, intraocular pressure, Latvian Hunting dogs, lens thickness, Schirmer tear test.

Assessment of the nutritive value of corn stover and king grass in complete feed on Ongole steer calves productivity

Research (Published online: 28-04-2020)

28. Assessment of the nutritive value of corn stover and king grass in complete feed on Ongole steer calves productivity

Ronny Agustinus Victor Tuturoong, Sjenny Sutryaty Malalantang and Sony Arthur Ely Moningkey

Veterinary World, 13(4): 801-806

ABSTRACT

Aim: This study aimed to assess the nutritional values of corn stover and king grass (Pennisetum purpupoides) in complete feed on the productivity of male Ongole steer calves.

Materials and Methods: This study was conducted in two steps: Cattle adaptation and data collection. Cattle adaptation was carried out for 2 weeks, and the cattle were fed the experimental feed. The experimental feed was formulated into five combinations: R1 (50% king grass); R2 (12.5% corn stover +7.5% king grass 50% concentrate); R3 (25% corn stover+25% king grass); R4 (37.5% corn stover+12.5% king grass); and R5 (50% corn stover). All experimental feeds were added with 50% concentrate. Data were collected in five intervals, and each interval was of 4 weeks. In every interval, weight gain and digestibility were measured every day, beginning from the 4th week by collecting feces. Dry matter (DM), organic matter (OM), crude protein (CP) digestibility, neutral detergent fiber (NDF), acid detergent fiber (ADF), body weight gain, and feed conversion data were analyzed.

Results: R4 and R5 treatment significantly increased (p<0.05) the feed nutritional value and weight gain of male Ongole steer calves. Interestingly, treatment with R4 had the maximum increase (p<0.05) on the digestibility value of each variable: DM, 68.85%; OM, 71.89%; CP, 73.90%; NDF, 59.10%; ADF, 55.35%; and weight gain, 0.61/gr/day.

Conclusion: R4 treatment found to be the best nutritional value for improving the productivity of male Ongole steer calves.

Keywords: complete feed, corn stover, king grass, Ongole breed.

Effect of mangosteen (Garcinia mangostana L.) peel extract as an antibiotic growth promoter on growth performance and antibiotic resistance in broilers

Research (Published online: 27-04-2020)

27. Effect of mangosteen (Garcinia mangostana L.) peel extract as an antibiotic growth promoter on growth performance and antibiotic resistance in broilers

Okti Herawati, Tri Untari, Marla Anggita and Sidna Artanto

Veterinary World, 13(4): 796-800

ABSTRACT

Background and Aim: Antibiotic resistance poses a risk to human health and has therefore been the focus of research. One of the causes of this resistance is the use of antibiotics as feed additives for animal nutrition. The development of antibiotic resistance in poultry through nutrition feed has drawn attention to the need for alternative antibiotic growth promoters (AGPs). Mangosteen (Garcinia mangostana L.), as a natural source of bioactive phytochemicals, is a potential AGP, but the effect of mangosteen-based treatment on antibiotic resistance in poultry has not been reported to date. Therefore, the aim of this study was to evaluate the effects of mangosteen peel extract as an AGP on body weight gain, feed conversion rate (FCR), and the antibiotic resistance in broilers.

Materials and Methods: In this study, 30 1-day-old broiler chicks were divided into three groups. Group A (control) was not administered any treatment in the feed, Group B was treated with 0.3 g/kg colistin as the AGP in the feed, and Group C was treated with 2% mangosteen peel extract as the AGP in the feed; the treatments were administered for 30 days. The observed parameters included the effect of the treatments on body weight gain, feed intake, FCR, and the presentation of antibiotic resistance before and after the treatments (pre-treatment and post-treatment, respectively).

Results: Post-treatment, the body weight gain, and feed intake in the broilers were not significantly different among all the groups; however, the body weight gain and FCR were significantly different between the control group and the treatment groups in the 3rd week of treatment and were not significantly different between Groups B and C. The rate of antibiotic resistance to chloramphenicol increased significantly by 40% in Group B post-treatment, but no such increase was observed in Groups A and C.

Conclusion: The findings of our study indicate that compared with using colistin as an AGP using mangosteen peel extract as a natural AGP did not have any significantly different effect on body weight gain, feed intake, and FCR (p>0.05) but had a significantly different effect on the rate of antibiotic resistance in broilers (p<0.05). This study indicates the usefulness of mangosteen for improving the overall growth and production performance of broilers without increasing their antibiotic resistance.

Keywords: antibiotic resistance, antibiotics, broiler, growth promoters, mangosteen.

Effects of using essential oil of Lavandula stoechas in quail feed on growth performance, carcass characteristics, meat quality, and health status

Research (Published online: 26-04-2020)

26. Effects of using essential oil of Lavandula stoechas in quail feed on growth performance, carcass characteristics, meat quality, and health status

Ouafa Laghouati, Fodil Arbouche and Yasmine Arbouche

Veterinary World, 13(4): 789-795

Aim: The aim of this study was to determine the effects of essential oil of Lavandula stoechas (Lavender) on growth performance, carcass characteristics, meat quality, and health status of quails.

Materials and Methods: A group of 600 1-day-old Japanese quail chicks (Coturnix japonica), with an average weight of 6.8±0.2 g and a 1:1 sex ratio, were randomly assigned to four groups (150 chicks/group): three experimental groups which depend on the incorporation of lavender oil (LO) in the different phases of breeding and one control group. The experimental groups received a supplement of 1 g LO per kg of feed distributed sequentially throughout the entire 42-day breeding period.

Results: No deaths were recorded throughout the breeding period. Highly significant differences were observed among the groups with regard to body weight measured on day 20 and day 42 (p<0.001 and p<0.01, respectively). The addition of LO was accompanied by reductions in liver weights; furthermore, LO had a significant effect on the pH, water content, and fat content of the meat (p<0.05). Administration of LO had a measurable effect on the endogenous intestinal population of Lactobacillus, and the bacterial load (including those of Escherichia coli and Staphylococcus aureus) was significantly reduced.

Conclusion: Our results demonstrate that supplementing quail feed with LO has a profound effect on their growth as well as antimicrobial effects.

Keywords: fat content, Japanese quail, lavender oil, microbial load, water content, zootechnical performance.

Lice infestation and diversity in turkeys (Meleagris gallopavo) in the Special Region of Yogyakarta and Central Java, Indonesia

Research (Published online: 25-04-2020)

25. Lice infestation and diversity in turkeys (Meleagris gallopavo) in the Special Region of Yogyakarta and Central Java, Indonesia

Joko Prastowo, Dwi Priyowidodo, Wisnu Nurcahyo, Defriana Lutfi Chusnaifah, Lu'lu' Sahara Wusahaningtyas, Lintang Winantya Firdausy and Ana Sahara

Veterinary World, 13(4): 782-788

ABSTRACT

Background and Aim: Biting lice (Phthiraptera: Amblycera and Ischnocera) are ectoparasites that play important roles in the transmission of disease agents that infect turkeys and impact turkey productivity. This study aimed to determine the diversity of lice that infest turkeys in the Central Java Province and the Special Region of Yogyakarta, Indonesia.

Materials and Methods: Lice sampling was conducted at 16 different locations from April 2019 to June 2019 in turkeys aged 4 months to 2 years. The samples were stored in 70% alcohol and were identified using avian louse keys. The morphology of the specimens was macroscopically and microscopically evaluated, and the resulting data were descriptively and qualitatively analyzed.

Results: A total of 2505 lice were collected, and two families and five genera of lice were identified. Three lice genus members of the Philopteridae family (Lipeurus, Oxylipeurus, and Chelopistes) and two genera of the Menoponidae family (Colpocephalum and Menacanthus) were identified. Lipeurus was the most frequently identified genera in turkeys, whereas Menacanthus was the most rarely identified one. The White Holland breed had the highest number of lice infestations, whereas the Jersey Buff breed exhibited the highest diversity of lice genera. The average number of lice infestations was higher in male turkeys than in female turkeys.

Conclusion: The occurrence of ectoparasites in domestic turkeys indicates that the existence and diversity of lice genera in the study location can be influenced by turkey type, turkey maintenance system, enclosure sanitation measures, lack of strategic ectoparasite control, and environmental factors.

Keywords: central java, lice, Phthiraptera, turkey, Yogyakarta.

Clinical and structural changes in reproductive organs and endocrine glands of sterile cows

Research (Published online: 24-04-2020)

24. Clinical and structural changes in reproductive organs and endocrine glands of sterile cows

Evgeny Skovorodin, Ravil Mustafin, Svetlana Bogoliuk, George Bazekin and Valian Gimranov

Veterinary World, 13(4): 774-781

ABSTRACT

Aim: The study aimed to determine both macroscopic and microscopic changes in the reproductive organs of sterile cows.

Materials and Methods: Careful clinical and gynecological examinations (using histological and histochemical methods) of organs of culled sterile cows, such as ovaries, oviducts, the uterus, pars anterior adenohypophysis, thyroid body, and adrenals, were performed.

Results: It was found that 20% of the examined cows in the farms of the Republic of Bashkortostan had pathology of reproductive organs. Ovarian dysfunction was diagnosed in 31% of sterile cows. Histological and histochemical studies revealed that high atresia of all types of ovarian follicles is associated with hypofunction of the ovaries. This was related to stromal vascular dystrophy and was accompanied by atrophy of thecal endocrine elements, resulting in decrease of endocrine and generative function of ovaries.

Conclusion: Essential elements of the ovarian dysfunction pathogenesis are disorders of the functional system "ovary-pituitary-adrenal-thyroid gland" and the abnormality of utero-ovarian relationships, which differ significantly during hypofunction, in case of follicular cysts, and in case of persistent corpora lutea. This difference in abnormalities of utero-ovarian relationships should be considered when developing diagnostic and treatment methods and determining preventive measures.

Keywords: cows, endocrine glands, morphology, ovaries, reproductive organs, sterility.

Growth performance and hematological changes in growing pigs treated with Cordyceps militaris spent mushroom substrate

Research (Published online: 23-04-2020)

23. Growth performance and hematological changes in growing pigs treated with Cordyceps militaris spent mushroom substrate

Waewaree Boontiam, Chalong Wachirapakorn and Suchat Wattanachai

Veterinary World, 13(4): 768-773

ABSTRACT

Aim: This study was aimed to compare the efficacy of dietary Cordyceps militaris spent mushroom substrate (CMS) on growth performance, immunity, metabolic profiles, and antioxidant capacity in growing pigs.

Materials and Methods: Seventy-two crossbred growing pigs (Duroc×Landrace×Yorkshire) with an average initial body weight (BW) of 25.78±0.33 kg were allotted into two dietary treatments in six pens (six growing pigs each). Dietary treatments were (i) control and (ii) supplemented group with 2 g/kg CMS.

Results: Growing pigs fed with 2 g/kg CMS showed improvements in final BW (p=0.034) and average daily weight gain (p=0.039). Moreover, there were positive changes in immunoglobulin A (p=0.013), immunoglobulin G (p=0.019), total antioxidant capacity (p=0.001), and glutathione peroxidase activity (p=0.003), whereas decreased leukocyte percentage (p=0.002), cholesterol (p=0.023), and malondialdehyde (MDA) concentrations (p=0.002) were noted in the CMS supplemented treatment. Average daily feed intake, gain-to-feed ratio, glucose, aspartate aminotransferase, triglyceride, high-density lipoprotein, and low-density lipoprotein were unaffected by the treatments.

Conclusion: Supplementation of CMS at 2 g/kg of diet increases growth performance, immunoglobulin secretion, and antioxidant capacity, whereas it lowers leukocyte percentage, cholesterol, and MDA concentrations in growing pigs.

Keywords: antioxidant capacity, blood metabolites, Cordyceps militaris spent substrate, growing pigs, growth performance, immunoglobulins.