Identification of Staphylococcus species isolated from preputium of Aceh cattle based on 16S rRNA gene sequences analysis

Research (Published online: 08-10-2019)

5. Identification of Staphylococcus species isolated from preputium of Aceh cattle based on 16S rRNA gene sequences analysis

Muhammad Hambal, Masda Admi, Safika Safika, Wahyu Eka Sari, Teuku Reza Ferasyi, Dasrul Dasrul, Ummu Balqis and Darmawi Darmawi

Veterinary World, 12(10): 1540-1545

ABSTRACT

Aim: This research aimed to identify Staphylococcus species isolated from preputial swabs of healthy Aceh cattle, based on 16S ribosomal RNA gene analysis.

Materials and Methods: The bacterium was isolated from preputial swabs of healthy Aceh cattle. The total DNA from the isolated bacteria was extracted using the Genomic DNA Mini Kit followed by polymerase chain reaction (PCR) amplification of the 16S rRNA gene. The product of PCR amplification was then sequenced and aligned to the known sequences in the GenBank database by multiple alignments and was also analyzed by bioinformatics software to construct a phylogenetic tree.

Results: The results revealed that the bacterial isolate 3A had genetically closed relation to Staphylococcus pasteuri with <97% maximum identity. Data derived from the phylogenetic tree revealed that the bacterial isolate 3A was also related to Staphylococcus warneri, yet, it shows a different evolutionary distance with the ancestors (S. pasteuri).

Conclusion: The results of this research suggested that the bacterium 3A, isolated from preputial swabs of healthy Aceh cattle, is a Staphylococcus species.

Keywords: 16S rRNA gene, Aceh cattle, phylogenetic tree, polymerase chain reaction, Staphylococcus pasteuri.

Antioxidative activity and protein profile of skim milk of Gaddi goats and hill cattle of North West Himalayan region

Research (Published online: 05-10-2019)

4. Antioxidative activity and protein profile of skim milk of Gaddi goats and hill cattle of North West Himalayan region

Vinesh Sharma, Birbal Singh, Rinku Sharma, Jyoti B. Dhar, Neelam Sharma and Gorakh Mal

Veterinary World, 12(10): 1535-1539

ABSTRACT

Aim: This study was aimed at evaluation of antioxidative activity, protein profile, and vitamins content of milk of Gaddi goats, local non-Gaddi goats, hill cattle, and Jersey crossbred cattle.

Materials and Methods: Total phenol, antioxidant activity measured as 2, 2-diphenyl- 1-picrylhydrazyl radical scavenging capacity, total protein, and vitamins were estimated in milk samples by spectrophotometric methods. Milk protein profiles were studied by sulfate-polyacrylamide gel electrophoresis.

Results: Total phenol, antioxidant activity, and total protein were higher in indigenous hill cattle skim milk. Average protein content in raw skimmed milk was 1.33±0.01, 1.03±0.02, 0.76±0.05, and 0.81±0.01%, in indigenous hill cattle, Jersey crossbred cattle, non-Gaddi goat, and Gaddi goat, respectively. Three proteins of 19.01, 22.08, and 32.96 kDa were observed in Gaddi goat, but not in non-Gaddi goat skim milk. Furthermore, the above proteins were absent in cattle skim milk. Two proteins of 15.56 and 25.06 kDa were found in local hill and crossbred cattle skimmed milk, but were absent in goat skimmed milk. Vitamin C content was the lowest in Gaddi goat milk and the highest in Jersey crossbred cattle milk.

Conclusion: It is envisaged that bioactive metabolites in the milk of Gaddi goats and hill cattle might offer anti-aging and beneficial health effects.

Keywords: antioxidants, Gaddi goats, hill cattle, milk proteins, skim milk, sulfate-polyacrylamide gel electrophoresis.

Immune response evaluation in Balb/c mice after crude extract of Anisakis typica sensitization

Research (Published online: 04-10-2019)

3. Immune response evaluation in Balb/c mice after crude extract of Anisakis typica sensitization

Linda Haryadi, Eddy Suprayitno, Aulanni'am Aulanni'am and Anik Martinah Hariati

Veterinary World, 12(10): 1529-1534

ABSTRACT

Background and Aim: Anisakis is a global challenge for a fish product which may lead to a decrease in economic value and consumers' preference. Skipjack (Katsuwonus pelamis) in Kupang, Nusa Tenggara Timur, Indonesia, have important economic value for local fisheries. Anisakis typica is one of the Anisakis species which potent to induce an allergic reaction. However, the study about A. typica involved in the dendritic cells (DCs), T helper 1 (Th1), T helper 2 (Th2), and regulatory T cells (Tregs) is still limited. This study aimed to analyze the dynamic changed of the immune system including DCs, CD4+ T cells, and Tregs after 1 week of A. typica sensitization.

Materials and Methods: Twenty-four male Balb/C mice were randomly divided into four groups (n=6), mice treated with crude A. typica extract (CAE) 50, 75, and 100 mg/kg BW, respectively. CAE was given orally per day for a week. At the end of the experiment, the animals were sacrificed and the spleen was collected. DCs were labeled as CD11c+ interleukin-6+ (IL-6+); CD4+ T cells were distinguished as Th1 (CD4+ interferon-γ+ [IFN-γ+]) and Th2 (CD4+ IL-4+ and CD4+ IL-5+); Tregs were labeled as CD4+CD25+CD62L+. The expression of each cell was determined by flow cytometry.

Results: Our result described that CAE elicits CD11c+ IL-6+, CD4+ IFN-γ+, CD4+ IL-4+, and CD4+ IL-5+ and reduces CD4+CD25+CD62L+ significantly (p<0.05) in dose-dependent manner in mice after A. typica infection.

Conclusion: The Th1/Th2 ratio after A. typica crude extract treatment exhibits a mixed pattern rather than the classical model allergy to food antigens. Our study is expected as a basic understanding of the changes in immune response after A. typica infection.

Keywords: allergy, dendritic cells, inflammation, nematode, regulatory T cells.

Detection of virulence and multidrug resistance operons in Pseudomonas aeruginosa isolated from Egyptian Baladi sheep and goat

Research (Published online: 04-10-2019)

2. Detection of virulence and multidrug resistance operons in Pseudomonas aeruginosa isolated from Egyptian Baladi sheep and goat

A. N. Dapgh, A. S. Hakim, H. A. Abouelhag, A. M. Abdou and E. A. Elgabry

Veterinary World, 12(10): 1524-1528

ABSTRACT

Background: Pseudomonas aeruginosa is a pit of an enormous group of free-living bacteria that are able to live everywhere and suggested to be the causative agent of great scope of acute and chronic animal infections.

Aim: The current study was carried out to illustrate the prevalence of P. aeruginosa in small ruminants and existence of some virulence operons as well as its antimicrobial resistance.

Materials and Methods: A total of 155 samples from sheep and 105 samples from goats (mouth abscesses, fecal swabs, nasal, tracheal swabs, and lung tissue) were collected for bacteriological study, existence of some virulence expression operons with the study of their sensitivity to the antimicrobials using disc diffusion and presence of mexR operon which is responsible for multidrug resistance (MDR).

Results: The bacteriological examination revealed that P. aeruginosa was isolated from nine out of 155 samples from sheep (5.8%) and four isolates out of 105 samples from goat (3.8%). It is found that 12 (92.3%), 10 (76.9 %), and 8 (61.5%) of P. aeruginosa isolates harbored hemolysin phospholipase gene (pclH), gene (exoS), and enterotoxin gene (toxA), respectively. The results of antibiotic sensitivity test showed that all tested isolates were resistant to ampicillin, bacitracin, erythromycin, streptomycin, tetracycline, trimethoprim-sulfamethoxazole, and tobramycin but sensitive to ciprofloxacin and norfloxacin. The MDR (mexR) operon was existed in all isolates.

Conclusion: There is a growing risk for isolation of virulent MDR P. aeruginosa from sheep and goat illness cases, and this should be regarded in the efficient control programs.

Keywords: drug resistance, goat, Pseudomonas aeruginosa, sheep, virulence.

Seroprevalence and factors associated with bovine and swine toxoplasmosis in Bobo-Dioulasso, Burkina Faso

Research (Published online: 02-10-2019)

1. Seroprevalence and factors associated with bovine and swine toxoplasmosis in Bobo-Dioulasso, Burkina Faso

Dieudonne Tialla, Laibane Dieudonne Dahourou and Oubri Bassa Gbati

Veterinary World, 12(10): 1519-1523

Background and Aim: Toxoplasmosis is a worldwide zoonosis with major public health importance. To know more about this condition in Burkina Faso, this study was implemented to determine the seroprevalence of Toxoplasma gondii infection among pigs and cattle in intra-urban and peri-urban area of Bobo-Dioulasso (Burkina Faso).

Materials and Methods: Serum samples were collected from 600 cattle and 600 pigs with 300 samples from each species in intra-urban and peri-urban area of Bobo-Dioulasso. Data about age, sex, and breed of each animal were also noted. Serum samples were analyzed by indirect enzyme-linked immunosorbent assay to look for immunoglobulin G and immunoglobulin M antibodies to T. gondii.

Results: This study revealed a herd prevalence of 92.5% and 75%, respectively for porcine and bovine toxoplasmosis. At the individual level, we found a prevalence of 29% and 49.2% for cattle and pigs, respectively. For each species, we noticed a significant association between age, sex, breed husbandry system, and the presence of anti-T. gondii antibodies. The prevalence was significantly higher in female, intra-urban system, exotic breed, and animal <2 years old (p<0.05).

Conclusion: The results provided evidence for the presence of T. gondii in pigs and cattle farms around Bobo-Dioulasso. Hence, in Bobo-Dioulasso, raw or undercooked meat consumption is a risk for T. gondii infection for human. Knowledge of the prevalence of toxoplasmosis will help to target prevention efforts.

Keywords: Burkina Faso, cattle, public health, seroepidemiologic studies, swine, toxoplasmosis.

Hematological and biochemical reference values of Asian house shrews (Suncus murinus) in Bangladesh

Research (Published online: 30-09-2019)

24. Hematological and biochemical reference values of Asian house shrews (Suncus murinus) in Bangladesh

Md. Kaisar Rahman, Shariful Islam, Mizanur Rahman, Jinnat Ferdous, Sazeda Akter, Md. Mustafizur Rahaman, Mohammad Alamgir Hossain, Mohammad Mahmudu Hassan and Ariful Islam

Veterinary World, 12(9): 1514-1518

ABSTRACT

Background and Aim: Determining reference values for hematological and biochemical parameters of Asian house shrew (Suncus murinus) is important for wildlife research to protect human health in surrounding communities. This study aimed to establish the reference values for selected hematology and serum clinical chemistry analyses that may contribute to research on shrew in future.

Materials and Methods: Blood samples (n=51) were collected from shrews between July and December 2015, Bangladesh, to estimate the levels of hemoglobin (Hb), packed cell volume (PCV), total leukocyte count (TLC), total erythrocyte count (TEC), lymphocyte, monocyte, neutrophil, eosinophil, basophil, calcium, phosphorus (P), sodium (Na), chloride (Cl), urea, glucose, total protein (TP), creatinine, and alanine transaminase (ALT).

Results: Although the values did not differ significantly among sexes, age was found to be a significant factor. Hb, PCV, TEC, glucose, and P were higher in males; eosinophil, Na, Cl, TP, and ALT were higher among females. Adults had significantly greater urea and glucose (p<0.05) while juveniles had insignificantly higher values for TLC, PCV, neutrophil, P, and TP.

Conclusion: This study provides the first reference values for this species in Bangladesh and can be used to guide wildlife research studies.

Keywords: Asian house shrew, hematology, reference interval, serum chemistry, Suncus murinus.

Molecular techniques for sex identification of captive birds

Research (Published online: 30-09-2019)

23. Molecular techniques for sex identification of captive birds

Medania Purwaningrum, Herjuno Ari Nugroho, Machmud Asvan, Karyanti Karyanti, Bertha Alviyanto, Randy Kusuma and Aris Haryanto

Veterinary World, 12(9): 1506-1513

ABSTRACT

Background and Aim: Many avian species are considered sexually monomorphic. In monomorphic bird species, especially in young birds, sex is difficult to identify based on an analysis of their external morphology. Accurate sex identification is essential for avian captive breeding and evolutionary studies. Methods with varying degrees of invasiveness such as vent sexing, laparoscopic surgery, steroid sexing, and chromosome inspection (karyotyping) are used for sex identification in monomorphic birds. This study aimed to assess the utility of a non-invasive molecular marker for gender identification in a variety of captive monomorphic birds, as a strategy for conservation.

Materials and Methods: DNA was isolated from feather samples from 52 individuals representing 16 species of 11 families indigenous to both Indonesia and elsewhere. We amplified the chromodomain helicase DNA-binding (CHD) gene using polymerase chain reaction with MP, NP, and PF primers to amplify introns with lengths that differ between the CHD-W and the CHD-Z genes, allowing sex discrimination because the W chromosome is exclusively present in females.

Results: Molecular bird sexing confirmed 33 females and 19 males with 100% accuracy. We used sequencing followed by alignment on one protected bird species (Probosciger aterrimus).

Conclusion: Sex identification may be accomplished noninvasively in birds, because males only have Z sex chromosomes, whereas females have both Z and W chromosomes. Consequently, the presence of a W-unique DNA sequence identifies an individual as female. Sexing of birds is vital for scientific research, and to increase the success rate of conservation breeding programs.

Keywords: bird, chromodomain helicase DNA-binding gene, molecular bird sexing, polymerase chain reaction, sexing.

Proteomics analysis of serum protein patterns in duck during aflatoxin B1 exposure

Research (Published online: 29-09-2019)

22. Proteomics analysis of serum protein patterns in duck during aflatoxin B1 exposure

Natthasit Tansakul, Jatuporn Rattanasrisomporn and Sittiruk Roytrakul

Veterinary World, 12(9): 1499-1505

ABSTRACT

Background and Aim: Unlike the already well-documented human serum proteome, there are still limitations regarding analyzing and interpreting the various physiological changes and disease states of the serum proteomes found in duck. Serum proteome in duck under the condition of mycotoxin contamination in feed has not yet been examined. This study aimed to introduce the characterization of the circulating proteomes in duck serum during exposure to aflatoxin B1 (AFB1).

Materials and Methods: Duck serum samples were collected from four experimental groups, gel-based mass spectrometry was then applied, and finally, 445 proteins were identified in pulled serum sample.

Results: Among these 445 proteins, 377 were present in at least one group from all. There were 35 proteins which were expressed when the duck was exposed to AFB1. The protein library that allows the identification of a large number of different proteins in duck serum will be enhanced by the addition of these peptide spectral data. It is noteworthy that chromodomain-helicase-DNA-binding protein 7 (CHD7) [Gallus gallus] was up-regulated in the group with the highest AFB1 contamination.

Conclusion: CHD7 protein might be somehow relative to aflatoxicosis in the duck that causes poor performance and economic loss. Moreover, other proteins present in duck serum were also added in the protein library.

Keywords: aflatoxin B1, duck, proteomics, serum.

Immune-inflammatory concept of the pathogenesis of chronic heart failure in dogs with dilated cardiomyopathy

Research (Published online: 28-09-2019)

21. Immune-inflammatory concept of the pathogenesis of chronic heart failure in dogs with dilated cardiomyopathy

Yu Vatnikov, A. Rudenko, P. Rudenko, Ev Kulikov, A. Karamyan, V. Lutsay, I. Medvedev, V. Byakhova, E. Krotova and M. Molvhanova

Veterinary World, 12(9): 1491-1498

ABSTRACT

Background: Dilated cardiomyopathy is common in dogs. This form of cardiomyopathy is the main cause of death due to heart disease in dogs. Death can occur suddenly in clinically normal animals as a result of the progression of congestive heart failure (CHF). The pathogenesis of heart failure syndrome in dogs with dilated cardiomyopathy involves activation of the neurohumoral system and immune-mediated inflammation, which leads to further progression of the condition. Heart failure syndrome in dogs with dilated cardiomyopathy is caused by the progressive loss of cardiomyocytes, apoptosis, remodeling of the left ventricle, systolic and diastolic dysfunction, arrhythmias, reduced cerebral blood flow, the involvement of other key internal organs, and intestinal dysbiosis.

Aim: This study aimed to determine the immunological and inflammatory mechanisms surrounding the development of heart failure syndrome in dogs with dilated cardiomyopathy.

Materials and Methods: The subjects of this study were dogs with a dilated form of cardiomyopathy (n=159), complicated by various functional classes of heart failure syndrome. Evaluation of myocardial remodeling, systolic function, and systemic hemodynamics was performed using EMP-860 Vet and PU-2200V ultrasound scanners according to the standard technique. Electrocardiography was performed with all dogs in right lateral recumbency using the EK1T-04 Midas electrocardiograph (50 mm/s speed and 1 mV gain = 1 cm).

Results: In some affected animals, especially in cases of compensated dilated cardiomyopathy, leukocytosis was noted. In patients with dilated cardiomyopathy complicated by heart failure syndrome of various functional classes, the number of neutrophils was significantly increased, and the number of lymphocytes was decreased by 1.9-2.1 times when compared with those in clinically normal animals. In dogs with dilated cardiomyopathy, neutrophilic leukocytosis develops with a simple regenerative shift to the left. The results of immunological studies indicate that dogs with dilated cardiomyopathy develop T lymphocytopenia as compared with clinically normal animals.

Conclusion: The central component of heart failure syndrome in dogs with dilated cardiomyopathy is the activation of the neurohumoral system and immune-mediated inflammation. The development of CHF in dogs with dilated cardiomyopathy is caused by the progressive loss of cardiomyocytes, apoptosis, remodeling of the left ventricle, systolic and diastolic dysfunction, arrhythmias, reduced cerebral blood flow, involvement of other key internal organs, and intestinal dysbiosis.

Keywords: dilated cardiomyopathy, dogs, pathogenesis, heart failure, immunity and inflammation.

Prevalence and distribution of dermatophytosis lesions on cattle in Plateau State, Nigeria

Research (Published online: 27-09-2019)

20. Prevalence and distribution of dermatophytosis lesions on cattle in Plateau State, Nigeria

J. S. Dalis, H. M. Kazeem, J. K. P. Kwaga and C. N. Kwanashie

Veterinary World, 12(9): 1484-1490

ABSTRACT

Background and Aim: Dermatophytosis is an infection of the superficial, keratinized structures of the skin, nails, and hair of man and animals caused by a group of fungi called dermatophytes in the genera Trichophyton, Microsporum, and Epidermophyton. The prevalence of dermatophytosis among cattle in Nigeria and Plateau State, in particular, is yet to be fully determined. This study aimed to determine the prevalence and the distribution of dermatophytosis lesions on cattle in Plateau State, Nigeria.

Materials and Methods: Four hundred and thirty-seven cattle showing visible skin lesions suggestive of dermatophytosis were drawn from nine local government areas (three each) from the three senatorial districts of Plateau State, Nigeria. Skin scrapings were aseptically collected using a cross-sectional study, in which sampling units were selected using purposive sampling method. Samples were processed for both direct microscopic examination and isolation of dermatophytes in culture. The isolates were stained with lactophenol cotton blue and identified microscopically based on the size, shape, and arrangement of macro- and micro-conidia. The dermatophytes were further identified by determining the sequences of the internal transcribed spacer regions of their ribosomal DNA. Data were analyzed and presented as percentages, bar graph, and Chi-square test of association. p≤0.05 was considered statistically significant.

Results: The overall prevalence rate of bovine dermatophytosis in Plateau State was found to be 11.0%. Trichophyton verrucosum was more frequently isolated (54.2%) than Trichophyton mentagrophytes (45.8%). Age, breed, management practice, and season were significantly associated with the occurrence of the disease (p<0.05).

Conclusion: Dermatophytosis among cattle may be of public health significance in Plateau State, Nigeria. This is the first report on the prevalence and distribution of dermatophytosis lesions on cattle from Plateau State, Nigeria.

Keywords: cattle, dermatophytes, identification, isolation, Nigeria, Plateau State.

In vitro evaluation of ruminant feed from West Sumatera based on chemical composition and content of rumen degradable and rumen undegradable proteins

Research (Published online: 27-09-2019)

19. In vitro evaluation of ruminant feed from West Sumatera based on chemical composition and content of rumen degradable and rumen undegradable proteins

Ezi Masdia Putri, Mardiati Zain, Lili Warly and Hermon Hermon

Veterinary World, 12(9): 1478-1483

ABSTRACT

Aim: This research aimed to discover the chemical composition, as well as the content of the degradable and undegradable protein of the ruminant feed commonly used as cattle feed by Indonesian farmers.

Materials and Methods: In this study, Pennisetum purpureum, Leucaena leucocephala, Indigofera zollingeriana, Gliricidia sepium, cassava, maize, palm kernel cake, and rice bran were used as feed. Chemical composition was determined by proximate and Van Soest analyses performed in triplicate. Dry matter and organic matter digestibility, as well as the rumen degradable proteins (RDP) and rumen undegradable proteins (RUP) contents, were determined in vitro using the Tilley and Terry method.

Results: The results showed that more proteins can be obtained from legumes than from grass or concentrates. The highest protein amount was obtained from I. zollingerian (31.22%), while the lowest protein amount was obtained from cassava (3.59%). Dry matter digestibility ranged from 18.53% (rice bran) to 49.21% (G. sepium). Organic matter digestibility ranged from 35.71% (cassava) to 59.57% (I. zollingerian). Rice bran had the highest RDP from concentrate (73.26%), whereas I. zollingerian had the highest RDP from forage (74.72%). The highest RUP from concentrate was obtained from palm kernel cake (61.01%), and the highest RUP from forage was obtained from L. leucocephala (49.23%).

Conclusion: The preparation of ruminant livestock rations must be based on RDP and RUP to meet the needs of both the rumen microbes and host animals. Information regarding the RDP and RUP of feeds is still limited, making this study useful in the preparation of ruminant livestock rations based on RDP and RUP ratios.

Keywords: chemical compositions, rumen degradable proteins, rumen undegradable proteins, ruminant, tropical feedstuff.

Immunopathological immunohistochemical study of low pathogenic avian influenza virus H5N1 infection in lovebirds (Agapornis spp.) in Indonesia

Research (Published online: 26-09-2019)

18. Immunopathological immunohistochemical study of low pathogenic avian influenza virus H5N1 infection in lovebirds (Agapornis spp.) in Indonesia

Zulfikhar Zulfikhar, Raden Wasito and Hastari Wuryastuti

Veterinary World, 12(9): 1472-1477

ABSTRACT

Background and Aim: To date, persistent infection with low pathogenic avian influenza virus (AIV) subtype H5N1 (LPAI H5N1) in chickens is widespread in Indonesia. Commercially available ornamental birds on markets play an important role in triggering the occurrence of AIV outbreaks. Thus, the presence of AIV in ornamental birds such as lovebirds needs to be closely monitored. Here, the presence of LPAI H5N1 was investigated in lovebirds with no clinical signs that, although apparently healthy, can be a continuous source of AIV transmission to other sensitive birds such as chickens and even humans.

Materials and Methods: In the present study, 30 lovebirds were necropsied. The immunopathology of the lungs, which had hemorrhages, was immunohistochemically studied using the streptavidin-biotin (SB) method to stain for LPAI H5N1. Polyclonal antibodies against the nucleoprotein or monoclonal antibodies against the hemagglutinin of the AIV subtype H5N1 were used to identify these antigens in lung tissue samples.

Results: LPAI H5N1 was detected in the lungs of all lovebirds according to the brownish colored deposits in the histological samples. The highest intensity of the SB staining was found in the lumina of vascular capillaries and the cell membranes of air capillaries. The discovery of LPAI H5N1 in lovebirds increases the environmental health risk and raises the possibility of exposure to AIV. Thus, it is very important to improve the mechanisms that control the traffic of ornamental birds between regions and islands, as well as the AIV vaccination strategies related to the prevention, control, and eradication of AIV in Indonesia, and these procedures should be reevaluated.

Conclusion: The present study proves that lovebirds are infected with LPAI subtype H5N1. Even if ornamental birds such as lovebirds do not show any clinical signs, they are likely to be a persistently infected with LPAI H5N1. Therefore, ornamental birds might be a continuous source of LPAI H5N1 infections in other sensitive birds, including poultry, and may also be responsible for virus transmission to humans.

Keywords: avian influenza virus H5N1, immunohistochemistry, Indonesia, lovebird, lung, streptavidin-biotin.

Detection of torque teno sus virus infection in Indian pigs

Research (Published online: 25-09-2019)

17. Detection of torque teno sus virus infection in Indian pigs

Vinutha Subramanyam, Divakar Hemadri, Shashidhara Phani Kashyap, Jagadish Hiremath, Nagendra Nath Barman, Esther Lalzoliani Ralte, Sharanagouda S. Patil, Kuralayanapalya P. Suresh and Habibur Rahaman

Veterinary World, 12(9): 1467-1471

ABSTRACT

Background and Aim: Torque teno viruses (TTVs) are circular, single-stranded DNA viruses, which infect a wide range of animals including livestock and companion animals. Swine TTVs (torque teno sus viruses [TTSuVs]) are thought to act as a primary or coinfecting pathogen in pathological conditions such as porcine dermatitis and nephropathy syndrome and post-weaning multisystemic wasting syndrome. So far, the presence of the virus has not been reported in India. Considering that TTSuVs have the potential to cross the species barrier into humans and that pork consumption is common in North-Eastern states of India, the current study aims to investigate the presence of TTSuV in the Indian pig population.

Materials and Methods: A total of 416 samples were collected during 2014-2018, from both apparently healthy pigs and also from pigs suspected of having died from classical swine fever and/or porcine reproductive and respiratory syndrome. These samples were screened for TTSuV infection by polymerase chain reaction (PCR) and DNA sequencing techniques.

Results: The presence of the virus was confirmed in 110 samples from 12 different states of India. Phylogenetic analysis of the nucleotide sequences obtained from the PCR products indicated the presence of viruses of both Iotatorquevirus and Kappatorquevirus genera in India.

Conclusion: The study is the first report on the presence of TTSuVs in India and highlights the circulation of both genera of the virus in the country.

Keywords: detection, India, phylogeny, polymerase chain reaction, torque teno virus.

The distribution of serotonergic nerve on the hippocampus of the fruit bats (Rousettus amplexicaudatus)

Research (Published online: 25-09-2019)

16. The distribution of serotonergic nerve on the hippocampus of the fruit bats (Rousettus amplexicaudatus)

Vivin Wirawati, Nourrisma D. A. Widiati, Geraldus Gunawan, Golda R. Saragih, Puspa Hening and Hevi Wihadmadyatami

Veterinary World, 12(9): 1460-1466

ABSTRACT

Background: Rousettus amplexicaudatus is one of the old world fruit bats, commonly known in Javanese word as codot belongs to Order Chiroptera and suborder Megachiroptera. R. amplexicaudatus possessed an outstanding visual ability, which helps in the process of searching for food. Those activities process and save within the brain involving the learning and memory activities. Learning and memory activities are centered around the hippocampus with the help of serotonergic nerve.

Aim: This study conducted to identify the morphology and distribution of serotonergic nerve (5-hydroxytryptamine) in the hippocampus of R. amplexicaudatus, which related to the function of serotonin within the learning and memory process.

Materials and Methods: Five R. amplexicaudatus bats were brought from Gunung Kidul cave, Yogyakarta, Java Island, Indonesia. The bats were anesthetized using ketamine and xylazine. R. amplexicaudatus then perfused intracardially. The brain samples were collected processed into paraffin block, and a further cut in 8 μm thickness serially. The tissue slides were stained using hematoxylin-eosin, cresyl echt violet, and immunohistochemistry with rabbit's anti-serotonin (1:200). The results observed and analyzed quantitatively and qualitatively using image J (https://imagej.nih.gov/ij/) (Bethesda, Maryland, USA) and GraphPad Prism 7 (La Jolla, CA, USA).

Results: The hippocampus of R. amplexicaudatus composed of the dentate gyrus (DG), hippocampus proper (cornu ammonis 3 [CA3], and CA1 as the main area for learning memory), and subiculum. On the DG serotonin immunoreactive cells found within the granular layer (132±35.03 cells/mm2), polymorphic stratum (86.33±11.23 cells/mm2), and molecular layer (93±1 cells/mm2). Meanwhile, on CA3 area, the immunoreactive cells for serotonin found in each stratum. The number of immunoreactive cells on each stratum from highest to the lowest are stratum pyramidal 123.33±15.88 cell/mm2, stratum molecular 63±13.11 cell/mm2, stratum lucidum 62.67±8.08 cell/mm2, stratum radiatum 55.33±510.21 cell/mm2, stratum oriens 48±3.46 cell/mm2, and stratum alveus 28.67±2.52 cell/mm2. In addition, in CA1 also hampers the immunoreactive cells in the pyramidal stratum, molecular, lucidum, oriens, radiatum, and alveus layer, respectively, of each 91±27.40 cell/mm2, 60.33±20.65 cell/mm2, 53.67±4.51 cell/mm2 44.33±10.40 cell/mm2, 41.33±5.51 cell/mm2, and 27±4 cell/mm2.

Conclusion: Taking together the distribution of serotonin-immunoreactive cells in the hippocampus of R. amplexicaudatus mostly found on CA3 followed by CA 1 and DG.

Keywords: hippocampus, immunohistochemistry, learning and memory, Rousettus amplexicaudatus, serotonin.

Detection of Leptospira spp. using polymerase chain reaction technique from kidney of Rattus norvegicus from Grenada, West Indies

Research (Published online: 24-09-2019)

12. Detection of Leptospira spp. using polymerase chain reaction technique from kidney of Rattus norvegicus from Grenada, West Indies

Bhumika Sharma, Katelyn Thille, Nia Rametta and Ravindra Sharma

International Journal of One Health, 5: 81-85

ABSTRACT

Aim: This study aimed to find out the prevalence of active infection of Leptospira spp. in Rattus norvegicus from Grenada, West Indies, through polymerase chain reaction (PCR).

Materials and Methods: One hundred and forty-nine rats were trapped, anesthetized and their kidneys collected aseptically. DNA was extracted from the kidney tissue of each rat. PCR was performed targeting LipL32 gene. Eighteen PCR-positive amplicons for LipL32 gene segment were purified and sent for direct sequencing to the sequencing facility of MCLAB (South San Francisco, USA). Results of sequencing were read and interpreted. The prevalence of Leptospira spp. in relation to sex and age was also recorded.

Results: All amplified sequences were compared to the sequences present in GenBank using basic local alignment search tool (BLAST) from the online website National Center for Biotechnology Information, the results revealed that six samples had similarity to Leptospira interrogans strain 1399/2016 and eight samples had similarity with Leptospira borgpetersenii serovar Hardjo-bovis strain L49. Of 149 kidney samples, only 14 were positive for Leptospira spp. by PCR giving an incidence of 9.3%. There was no significant difference found in relation to sex and age.

Conclusion: This is the first report confirming active infection of Leptospira spp. in Rattus norvegicus in Grenada using PCR. The presence of active infection in rats can be considered as high risk for humans. Further research to understand the epidemiology of leptospirosis in Grenada is suggested.

Keywords: Rattus norvegicus, Grenada, kidney, Leptospira spp., polymerase chain reaction.

Prevalence and species identification of Cryptosporidium spp. in the newborn dairy calves from Muang District, Khon Kaen Province, Thailand

Research (Published online: 23-09-2019)

15. Prevalence and species identification of Cryptosporidium spp. in the newborn dairy calves from Muang District, Khon Kaen Province, Thailand

Phennarin Doungmala, Patchara Phuektes, Weerapol Taweenan, Somboon Sangmaneedet and Ornampai Japa

Veterinary World, 12(9): 1454-1459

ABSTRACT

Aim: This study aims to determine the prevalence of Cryptosporidium spp. infection and to identify the species of Cryptosporidium spp. in newborn dairy calves between December 2016 and March 2017 in Muang District, Khon Kaen Province, Thailand.

Materials and Methods: A total of 200 fecal samples from newborn dairy calves of the ages 1 day up to 28 days were collected and the presence of Cryptosporidium oocysts was examined microscopically using the modified Kinyoun's acid-fast staining technique. Then, Cryptosporidium species were identified using nested polymerase chain reaction amplification of 18S rRNA gene and sequencing.

Results: The modified Kinyoun's acid-fast staining revealed the presence of Cryptosporidium oocysts in 51% (102/200). Sequence analysis of the 18S rRNA gene identified two species, namely, Cryptosporidium bovis (n=11) and Cryptosporidium ryanae (n=11) and one isolated strain could not be identified.

Conclusion: This study indicated that newborn dairy calves aging up to 4 weeks were highly infected with Cryptosporidium spp., and the infection mostly occurred in diarrheic dairy calves. This is the first report of Cryptosporidium in dairy calves in Khon Kaen Province and the results provide baseline information for further studies and control of Cryptosporidium infection in dairy calves in the study area.

Keywords: Cryptosporidium, Khon Kaen Province, nested polymerase chain reaction, newborn dairy calves, prevalence.

Modification on acute myocardial infarction model through left anterior descending coronary artery ligation: An experimental study on rats (Rattus norvegicus) using readily available materials

Research (Published online: 21-09-2019)

14. Modification on acute myocardial infarction model through left anterior descending coronary artery ligation: An experimental study on rats (Rattus norvegicus) using readily available materials

Johanes Nugroho, Wiwik Misaco Yuniarti, Ardyan Wardhana and Cornelia Ghea

Veterinary World, 12(9): 1448-1453

ABSTRACT

Background and Aim: Several difficulties are involved in creating models for myocardial infarction (MI) in animals, such as low survival rates after acute MI, complicated techniques in creating animal models, complexities in confirming acute MI incidence, and complex surgical tools needed in the process. This study aimed to develop an animal model for acute MI using Wistar rats utilizing simple instruments that are readily available in standard animal laboratories.

Materials and Methods: We induced MI in 48 Wistar rats using the left anterior descending coronary artery ligation modification technique without tracheal incision and ventilator. This ligation technique was performed 1-2 mm distal to the left atrial appendage. MI occurrence was evaluated using heart enzyme parameters 24 h post-ligation and histological studies of the infarcted area 6 weeks after the ligation. Rats were divided into the coronary artery ligation group and sham group.

Results: Of the 48 rats, 24 (50%) died within 24 h post-ligation, but no further deaths occurred in the next follow-up period of 6 weeks. The average infarct size in six rats within 24 h of ligation was 35%±5.7%. The serum glutamic oxaloacetic transaminase level of the group treated with coronary artery ligation was statistically significantly higher than that of the sham group (p=0.000).

Conclusion: We developed an MI rat model with consistent infarction size, in which the long-term death of rats was not observed. Our ligation technique for an MI rat model can be a reference for experimental settings without ventilators for small animals.

Keywords: left anterior descending coronary artery ligation, myocardial infarction, rat model.

Assessing farmers' perspective on antibiotic usage and management practices in small-scale layer farms of Mymensingh district, Bangladesh

Research (Published online: 19-09-2019)

13. Assessing farmers' perspective on antibiotic usage and management practices in small-scale layer farms of Mymensingh district, Bangladesh

Jannatul Ferdous, Sabbya Sachi, Zakaria Al Noman, S. M. Azizul Karim Hussani, Yousuf Ali Sarker and Mahmudul Hasan Sikder

Veterinary World, 12(9): 1441-1447

Background and Aim: Indiscriminate and injudicious use of antibiotics in layer farms is a common practice of Bangladesh for the compensation of Poor management practices and ignorance. Despite this scenario, there is no published documentation on antibiotic usage pattern and farm management practices in layer farms. This study was undertaken to understand the farmers' perspective in small-scale layer farms regarding antibiotics usage and farm management.

Materials and Methods: A questionnaire survey was conducted in 120 small-scale layer farms of Mymensingh district during January-February 2017. We only considered farms in production. Data were analyzed on antibiotic usage, purpose, egg management, understanding of antibiotic residue, withdrawal period, and other issues.

Results: Among 120 farmers, about 94.16% of farmers are using antibiotics without respecting the withdrawal period. Only 39.1% of farmers possess knowledge of residues. In our surveyed farms, 91.83% of farmers are not practicing egg washing before supplying to the market and 52.67% of farmers are unaware of cleaning and disinfection of egg tray. Ten different types of antibiotics of seven classes have found in the survey. Most antibiotics are in the Watch (49%) and Reserve (8%) groups according to the WHO AWaRe categorization and 73% antibiotics are critically important for human medicine and are considered as last resort.

Conclusion: This study found that due to the lack of knowledge and poor management, farmers consider using antibiotics as the most effective practices to control disease and enhancement of egg production. These indiscriminate uses of antibiotics are responsible for antibiotic residual and resistance problem. Here, we also provide some suggestion and guidelines to improve management practices to minimize the emerging problems of antimicrobial resistance through small-scale layer farms.

Keywords: antibiotic usage, AWaRe categorization, layer farms, management practices.