Detection and characterization of pathogenic Pseudomonas aeruginosa from bovine subclinical mastitis in West Bengal, India

Research (Published online: 04-07-2017)

4. Detection and characterization of pathogenic Pseudomonas aeruginosa from bovine subclinical mastitis in West Bengal, India

S. Banerjee, K. Batabyal, S. N. Joardar, D. P. Isore, S. Dey, I. Samanta, T. K. Samanta and S. Murmu

Veterinary World, 10(7): 738-742

ABSTRACT

Aim: Subclinical mastitis in bovines is mainly responsible for the huge economic loss of the dairy farmers, of which Pseudomonas aeruginosa is one of the causative agents. The study was aimed at a screening of suspected milk samples from different cattle farms of West Bengal for detection and confirmation of P. aeruginosa strains followed by their characterization.

Materials and Methods: Around 422 milk samples were screened from different dairy farms primarily by on-spot bromothymol blue (BTB) test and then in the lab by somatic cell counts (SCC) to finally consider 352 samples for detection of P. aeruginosa. Selective isolation and confirmation of the isolates were done using selective media, viz., cetrimide and Pseudomonas agar followed by confirmation by fluorescent technique. Molecular characterization of the strains was done by polymerase chain reaction for the presence of toxA (enterotoxin A, 352 bp) and exoS (exoenzyme S, 504 bp) genes.

Results: Approximately, 371 (87.9%) samples were positive in on-spot BTB test among which 352 (94.8%) samples revealed high SCC values (more than 3 lakh cells/ml) showing infection when screened. Among these, 23 (6.5%) samples yielded typical Pseudomonas sp. isolates out of which only 19 (5.4%) isolates were confirmed to be P. aeruginosa which showed characteristic blue-green fluorescence due to the presence of pigment pyoverdin under ultraviolet light. Out of these 19 isolates, 11 isolates were positive for toxA, 6 isolates for exoS, and 2 for both these pathogenic genes.

Conclusion: Approximately, 5.4% cases of bovine subclinical mastitis infections in South Bengal were associated with P. aeruginosa which possess pathogenic genes such as toxA (63.2%) and exoS (36.8%).

Keywords: bovines, characterization, exoS, Pseudomonas aeruginosa, subclinical mastitis, toxA.

Cuticular surface damage of Ascaridia galli adult worms treated with Veitchia merrillii betel nuts extract in vitro

Research (Published online: 03-07-2017)

3. Cuticular surface damage of Ascaridia galli adult worms treated with Veitchia merrillii betel nuts extract in vitro

Ummu Balqis, Muhammad Hambal, Rinidar, Farida Athaillah, Ismail, Azhar, Henni Vanda and Darmawi

Veterinary World, 10(7): 732-737

ABSTRACT

Aim: The objective of this research was to in vitro evaluate the cuticular surface damage of Ascaridia galli adult worms treated with ethanolic extract of betel nuts Veitchia merrillii.

Materials and Methods: Phytochemical screening was done using FeCl3, Wagner and Dragendorff reagents, NaOH, MgHCl, and Liebermann-Burchard reaction test. Amount of 16 worms were segregated into four groups with three replicates. Four worms of each group submerged into phosphate buffered saline, 25 mg/ml, and 75 mg/ml crude ethanolic extract of V. merrillii, and 15 mg/ml albendazole. The effect of these extract was observed 40 h after incubation as soon as worms death. The worms were sectioned transversally and were explored for any cuticular histopathological changes in their body surface under microscope.

Results: We found that the ethanolic extract of V. merrillii betel nuts contains tannins, alkaloids, flavonoids, triterpenoids, and saponins. The ethanolic extract of betel nuts V. merrillii induces surface alterations caused cuticular damage of A. galli adult worms.

Conclusion: We concluded that ethanolic extract of betel nuts V. merrillii possess anthelmintic activity caused cuticular damage of A. galli adult worms.

Keywords: anthelmintics, Ascaridia galli, cuticle, Veitchia merrillii.

Performance of broiler chicken fed multicarbohydrases supplemented low energy diet

2. Performance of broiler chicken fed multicarbohydrases supplemented low energy diet

Kumar Govil, Sunil Nayak, R. P. S. Baghel, A. K. Patil, C. D. Malapure and Dinesh Thakur

Veterinary World, 10(7): 727-731

doi: 10.14202/vetworld.2017.727-731

Abstract

Aim: Objective of this study was to investigate the effect of multicarbohydrases supplementation on performance of broilers fed low energy diet.

Materials and Methods: A total of 75 days old chicks were selected and randomly divided into three treatments groups (T₁, T₂, and T₃); each group contained 25 chicks distributed in five replicates of five chicks each. T₁ group (positive control) was offered control ration formulated as per Bureau of Indian Standards recommendations. In T₂ group (negative control) ration, metabolizable energy (ME) was reduced by 100 kcal/kg diet. T₃ group ration was same as that of T₂ except that it was supplemented with multicarbohydrases (xylanase at 50 g/ton+mannanase at 50 g/ton+amylase at 40 g/ton). Feed intake and body weight of all experimental birds were recorded weekly. Metabolic trial was conducted for 3 days at the end of experiment to know the retention of nutrients.

Results: Significant improvement (p<0.01) was observed in total weight gain, feed conversion efficiency, and performance index in broilers under supplementary group T₃ as compared to T₁ and T₂ groups. Retention of crude protein and ether extract was significantly increased (p<0.05) in T₃ group supplemented with multicarbohydrases as compared to other groups. Retention of dry matter, crude fiber, and nitrogen-free extract was comparable in all the three groups. Significantly highest dressed weight, eviscerated weight, and drawn weight (% of live body weight) were observed in multicarbohydrases supplemented T₃ group, however it was comparable in T₁ and T₂ groups.

Conclusion: It was concluded that the supplementation of multicarbohydrases (xylanase at 50 g/ton+mannanase at 50 g/ton+amylase at 40 g/ton) in low energy diet improved overall performance of broilers.

Keywords: broiler, carcass traits, multicarbohydrases, performance.

Screening method for Staphylococcus aureus identification in subclinical bovine mastitis from dairy farms

Research (Published online: 01-07-2017)

1. Screening method for Staphylococcus aureus identification in subclinical bovine mastitis 

from dairy farms

Natapol Pumipuntu, Suphang Kulpeanprasit, Sirijan Santajit, Witawat Tunyong, Thida Kong-ngoen, 

Woranich Hinthong and Nitaya Indrawattana

Veterinary World, 10(7): 721-726

ABSTRACT

Background: Staphylococcus aureus is one of the most important contagious bacteria causing subclinical bovine mastitis. This bacterial infection is commonly identified by determine the pathogen in bovine milk samples through conventional technique including coagulase test. However, this test has several disadvantages as low sensitivity, risk of biohazard, cost expensive, and limited preparation especially in local area.

Aim: Aim of this study was to compare and assess the screening method, Mannitol fermentation test (Mannitol salt agar [MSA]), and deoxyribonuclease (DNase) test, for S. aureus identification in milk samples.

Materials and Methods: A total of 224 subclinical bovine mastitis milk samples were collected from four provinces of Thailand and determined S. aureus using conventional method and also subjected to the screening test, MSA and DNase test. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) among both tests were analyzed and compared to the tube coagulase test (TCT), as reference method. Immunological test by latex agglutination and molecular assay by determined spa gene were also used to identify and differentiate S. aureus.

Results: A total of 130 staphylococci were isolated by selective media, Gram-stain, and catalase test. The number of S. aureus which identified using TCT, MSA and DNase test were 32, 102, and 74 isolates, respectively. All TCT results were correlated to results of latex agglutination and spa gene which were 32 S. aureus. MSA showed 100% sensitivity, 28.57% specificity, 31.37% PPV, and 100% NPV, whereas DNase showed 53.13% sensitivity, 41.84% specificity, 22.97% PPV, and 73.21% NPV. DNase test showed higher specificity value than MSA but the test presented 26.79% false negative results whereas no false-negative result from MSA when comparing to TCT.

Conclusion: MSA had a tendency to be a good preference for screening S. aureus because of its high sensitivity and NPV. The result from this study will improve a choice to use a screening test to diagnose S. aureus of veterinary field for prompt disease controlling and effective treatment.

Keywords: bovine mastitis, deoxyribonuclease test, mannitol fermentation test, screening methods, Staphylococcus aureus.

Virulence gene profiles of Arcobacter species isolated from animals, foods of animal origin, and humans in Andhra Pradesh, India

Research (Published online: 30-06-2017)

23. Virulence gene profiles of Arcobacter species isolated from animals, foods of animal origin, and humans in Andhra Pradesh, India

M. Soma Sekhar, S. R. Tumati, B. K. Chinnam, V. S. Kothapalli and N. Mohammad Sharif

Veterinary World, 10(6): 716-720

ABSTRACT

Aim: This study aimed to detect putative virulence genes in Arcobacter species of animal and human origin.

Materials and Methods: A total of 41 Arcobacter isolates (16 Arcobacter butzleri, 13 Arcobacter cryaerophilus, and 12 Arcobacter skirrowii) isolated from diverse sources such as fecal swabs of livestock (21), raw foods of animal origin (13), and human stool samples (7) were subjected to a set of six uniplex polymerase chain reaction assays targeting Arcobacter putative virulence genes (ciaB, pldA, tlyA, mviN, cadF, and cj1349).

Results: All the six virulence genes were detected among all the 16 A. butzleri isolates. Among the 13 A. cryaerophilus isolates, cadF, ciaB, cj1349, mviN, pldA, and tlyA genes were detected in 61.5, 84.6, 76.9, 76.9, 61.5, and 61.5% of isolates, respectively. Among the 12 A. skirrowii isolates, cadF, ciaB, cj1349, mviN, pldA, and tlyA genes were detected in 50.0, 91.6, 83.3, 66.6, 50, and 50% of isolates, respectively.

Conclusion: Putative virulence genes were detected in majority of the Arcobacter isolates examined. The results signify the potential of Arcobacter species as an emerging foodborne pathogen.

Keywords: Arcobacter, Arcobacter butzleri, Arcobacter cryaerophilus, Arcobacter skirrowii, polymerase chain reaction, virulence genes.

Honey can repairing damage of liver tissue due to protein energy malnutrition through induction of endogenous stem cells

Research (Published online: 29-06-2017)

22. Honey can repairing damage of liver tissue due to protein energy malnutrition through induction 

of endogenous stem cells

R. Heru Prasetyo and Eka Pramyrtha Hestianah

Veterinary World, 10(6): 711-715

ABSTRACT

Aim: This study was to evaluate effect of honey in repairing damage of liver tissue due to energy protein malnutrition and in mobilization of endogenous stem cells.

Materials and Methods: Male mice model of degenerative liver was obtained through food fasting but still have drinking water for 5 days. It caused energy protein malnutrition and damage of liver tissue. The administration of 50% (v/v) honey was performed for 10 consecutive days, while the positive control group was fasted and not given honey and the negative control not fasted and without honey. Observations of regeneration the liver tissue based on histologically examination, observation of Hsp70 expression, and homing signal based on vascular endothelial growth factor-1 (VEGF-1) expression using immunohistochemistry technique. Observation on expression of CD34 and CD45 as the marker of auto mobilization of hematopoietic stem cells using flow cytometry technique.

Results: There is regeneration of the liver tissue due to protein energy malnutrition, decrease of Hsp70 expression, increase of VEGF-1 expression, and high expression of CD34 and CD45.

Conclusion: Honey can improve the liver tissue based on: (1) Mobilization of endogenous stem cells (CD34 and CD45); (2) Hsp70 and VEGF-1 expressions as regeneration marker of improvement, and (3) regeneration histologically of liver tissue.

Keywords: endogenous stem cells, honey, liver tissue, protein energy malnutrition, regeneration.

Tetracycline resistance phenotypes and genotypes of coagulase-negative staphylococcal isolates from bubaline mastitis in Egypt

Research (Published online: 25-06-2017)

21. Tetracycline resistance phenotypes and genotypes of coagulase-negative staphylococcal 

isolates from bubaline mastitis in Egypt

K. A. Abd El-Razik, A. A. Arafa, R. H. Hedia and E. S. Ibrahim

Veterinary World, 10(6): 702-710

ABSTRACT

Aim: This study was devoted to elucidate the tetracycline resistance of coagulase-negative staphylococci (CNS) derived from normal and subclinical mastitic (SCM) buffaloes' milk in Egypt.

Materials and Methods: A total of 81 milk samples from 46 normal buffalo milk samples and 35 SCM buffalo milk samples at private dairy farms of Egypt were used in this study. CNS were identified using phenotypic and molecular methods (polymerase chain reaction [PCR]). CNS isolates were tested for tetracycline resistance using routine methods and multiplex PCR targeting tetracycline (tet) resistance genes followed by sequencing of positive PCR products and phylogenetic analysis.

Results: Isolation and identification of 28 (34.5%) CNS from normal and SCM buffaloes' milk, namely, Staphylococcus intermedius (39.2%), Staphylococcus xylosus (25.0%), Staphylococcus epidermidis (10.7%), Staphylococcus hominis (10.7%), and 3.5% to each of Staphylococcus sciuri, Staphylococcus hyicus, Staphylococcus lugdunensis, and Staphylococcus simulans. Using nested PCR, all the 28 CNS isolates revealed positive for 16srRNA gene specific for genus staphylococci and negative for thermonuclease (nuc) gene specific for Staphylococcus aureus species. The presence of tetracycline resistance-encoding genes (tetK, tetL, tetM, and tetO) was detected by multiplex PCR. All isolates were negative for tetL, M, and O genes while 14 (50%) CNS isolates were positive for tetK gene, namely, S. lugdunensis (100%), S. hominis (100%), S. epidermidis (66.6%), S. intermedius(45.4%), and S. xylosus (42.8%). Nucleotide sequencing of tetK gene followed by phylogenetic analysis showed the high homology between our CNS isolates genes of tetracycline resistance with S. aureus isolates including Egyptian ones. This proves the transfer of the tetracycline resistance encoding genes between coagulase-negative and coagulase-positive Staphylococcus spp.

Conclusion: CNS isolates have distinguishingly high resistance to tetracycline. Abundant tetracycline usage for mastitis treatment leads to the spread of genetic resistance mechanisms inside CNS strains and among all Staphylococcus spp. Consequently, tetracycline is not effective anymore.

Keywords: buffaloes, coagulase-negative staphylococci, mastitis, tetracycline resistance, tetK gene.

Investigation of avian influenza infection in wild birds in Ismailia and Damietta cities, Egypt

Research (Published online: 25-06-2017)

20. Investigation of avian influenza infection in wild birds in Ismailia and Damietta cities, Egypt

Hanaa Mohamed Fadel and Rabab Afifi

Veterinary World, 10(6): 695-701

ABSTRACT

Aim: This study was carried out to monitor avian influenza (AI) infection in wild birds in Egypt.

Materials and Methods: A total of 135 wild birds were examined for the presence of H5, H7, and H9 hemagglutination inhibition antibodies. Organs and swab samples of 75 birds were screened by multiplex real-time reverse transcriptase-polymerase chain reaction (RRT-PCR) to detect AI subtypes H5, H7, and H9 matrix genes.

Results: The highest seropositive result was recorded in cattle egrets (90.9%) followed by crows (88.6%), semi-captive pigeons (44.8%), and moorhens (39.1%). In cattle egrets, semi-captive pigeons and moorhens, H5 antibodies predominated. In crows, H9 antibodies predominated. Multiple infections with two or three virus subtypes were highest in crows (6/39, 15.4%) followed by cattle egrets (3/30, 10%) and moorhens' (1/9, 11.1%) positive samples. Multiplex RRT-PCR results revealed two positive samples in cattle egrets and moorhens.

Conclusion: The results indicated high seropositive rates against AI virus subtypes H5 and H9 in the examined wild birds. Multiple infections with more than one AI virus (AIV) subtypes were detected in some birds. This requires a collaboration of efforts to monitor AIV infection in wild birds and implement suitable early intervention measures.

Keywords: avian influenza, hemagglutination inhibition, real-time reverse transcriptase-polymerase chain reaction, wild birds.

Epidemiological study of tick infestation in buffalo of various regions of district Khairpur, Pakistan

Research (Published online: 24-06-2017)

19. Epidemiological study of tick infestation in buffalo of various regions of district Khairpur, Pakistan

Farzana Abbasi, Imtiaz Hussain Raja Abbasi, Tahmeena Fakhur Nissa, Zohaib Ahmed Bhutto, Muhammed Asif Arain, Rab Nawaz Soomro, Farman Ali Siyal and Sarfraz Ali Fazlani

Veterinary World, 10(6): 688-694

doi: 10.14202/vetworld.2017.688-694

Abstract

Aim: The aim of this study was to determine the epidemiological infestation and identification of Ixodidae and Argasidae ticks species in buffalo of different parts of district Khairpur, Pakistan.

Materials and Methods: A total of 720 Water buffaloes from three tehsils (subdivisions) were selected randomly and examined from organized and unorganized dairy farms for tick infestation in district Khairpur, Pakistan. This epidemiological survey was conducted during April to September 2015.

Results: The overall mean population and preferred site of tick attachment to infested animals, in Gambat, Sobhodero, and Kot Diji tehsils, were observed on different body parts. The primary body area of infestation by ticks (head, thorax, abdomen, udder, and tail) ranged from highest in tail and udder part compared to lowest in the abdomen, head, and thorax. In all study areas, the infestation was higher (p<0.05) in tail and udder than other parts of the body. In all the study areas, the overall highest population was found in the month of July. In addition, we first time identify four new species of ticks (Hyalomma anatolicum, H. anatolicum excavatum, Hyalomma Ixodes excavatum, and Ixodes ricinus) in district Khairpur, Pakistan.

Conclusion: Results of this study provide additional information of epidemiological tick infestation, and will be helpful for evolving effective control policy for the management of tick infestation in study district.

Keywords: Argasidae, buffalo, identification, Ixodidae, population, prevalence, ticks, tick infestation.

Th1/Th2 balance and humoral immune response to potential antigens as early diagnostic method of equine Strongylus nematode infection

Research (Published online: 24-06-2017)

18. Th1/Th2 balance and humoral immune response to potential antigens as early diagnostic method of equine Strongylus nematode infection

Faten A. M. Abo-Aziza, Seham H. M. Hendawy, Amira H. El Namaky and Heba M. Ashry

Veterinary World, 10(6): 679-687

doi: 10.14202/vetworld.2017.679-687

Abstract

Aim: The aim of this study was to investigate the early diagnosis of strongyle infection based on early changes in Th1 and Th2 cytokines beside the diagnostic accuracy values and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting profiles using prepared strongyles antigens.

Materials and Methods: A total of 73 donkeys had a mean age of 4-32 years old were parasitologically examined for strongyle infection. The early changes in Th1 and Th2 cytokines were determined, and the diagnostic accuracy values and SDS-PAGE and western blotting profiles were performed using prepared strongyles antigens; crude somatic Strongylus vulgaris (CSS), excretory-secretory S. vulgaris (ESS), crude somatic Cyathostomins (CSC), and excretory-secretory Cyathostomins (ESC).

Results: The results revealed highest 437.04% and lowest 37.81% immunoglobulin G (IgG) in high and low egg shedder groups when using ESC and CSS antigens, respectively. Antibodies index for ESS and CSC were significantly higher in moderate egg shedder group while that for ESS and CSC, ESC was significantly higher in high egg shedder group. Tumor necrosis factor alpha (TNF-α)/interleukin-4 (IL-4) balance in S. vulgaris infected donkeys was approximately equal in apparently healthy, low and high egg shedder groups while TNF-α < IL-4 in moderate egg shedder. In Cyathostomins infected animals, TNF-α/IL-4 balance was approximately equal in apparently healthy group while it was low in moderate and high egg shedder groups. The diagnostic accuracy showed that the higher specificity (46.6%) and prevalence (95.40%) were recorded by CSS and ESC antigens, respectively. However, SDS-PAGE and western blotting profiling proved that the band at molecular weight 25 kDa is exhibited by CSS antigen.

Conclusion: Combination of detecting level of TNF-α/IL-4 balance, CSS antigen and IgG concentration is good tool for appropriate diagnosis of such infection. More advancement research must be done concerning Th1/Th2 balance and cross-reactivity of S. vulgaris and Cyathostomins spp. at the base of serological and molecular investigation.

Keywords: cytokines, interleukin-4, Strongylus, Th1, Th2, tumor necrosis factor alpha.

Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen

Research (Published online: 20-06-2017)

17. Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen

A. A. Tarig, H. Wahid, Y. Rosnina, N. Yimer, Y. M. Goh, F. H. Baiee, A. M. Khumran, H. Salman, M. A. Assi and M. Ebrahimi

Veterinary World, 10(6): 672-678

ABSTRACT

Aim: The objective of this study was to evaluate the effects of different concentrations of soybean lecithin (SL) and virgin coconut oil (VCO) in Tris-based extender on chilled and frozen-thawed bull semen quality parameters.

Materials and Methods: A total of 24 ejaculates were collected from four bulls via an electroejaculator. Semen samples were diluted with 2% VCO in Tris-based extender which consists of various concentrations of SL (1, 1.25, 1.5, and 1.75%). A 20% egg yolk in Tris used as a positive control (C+). The diluted semen samples were divided into two fractions; one for chilling which were stored at 4°C for 24, 72, and 144 h before evaluated for semen quality parameters. The second fraction used for freezing was chilled for 3 h at 4°C, packed into 0.25 mL straws and then cryopreserved in liquid nitrogen. The samples were then evaluated after 7 and 14 days. Chilled and frozen semen samples were thawed at 37°C and assessed for general motility using computer-assisted semen analysis, viability, acrosome integrity and morphology (eosin-nigrosin stain), membrane integrity, and lipid peroxidation using thiobarbituric acid reaction test.

Results: The results showed that all the quality parameters assessed were significantly (p<0.05) improved at 1.5% SL concentration in chilled semen. Treatment groups of 1, 1.25, 1.5, and 1.75% SL were higher in quality parameters than the control group (C+) in chilled semen. However, all the quality parameters in frozen-thawed semen were significantly higher in the C+ than the treated groups.

Conclusion: In conclusion, supplementation of 1.5% SL in 2% VCO Tris-based extender enhanced the chilled bull semen. However, there was no marked improvement in the frozen-thawed quality parameters after treatment.

Keywords: bull semen, cryopreservation, quality parameters, soybean lecithin, virgin coconut oil.

Coxiellosis in domestic livestock of Puducherry and Tamil Nadu: Detection of Coxiella burnetii DNA by polymerase chain reaction in slaughtered ruminants

Research (Published online: 19-06-2017)

16. Coxiellosis in domestic livestock of Puducherry and Tamil Nadu: Detection of 

Coxiella burnetii DNA by polymerase chain reaction in slaughtered ruminants

Jothimani Pradeep, Selvaraj Stephen, Pratheesh Pooja, Anbalagan Akshayavardhini, Balakrishnan Sangeetha and Prabakar Xavier Antony

Veterinary World, 10(6): 667-671

ABSTRACT

Background and Aim: In the course of our Indian Council of Medical Research project on coxiellosis in Puducherry and Tamil Nadu, 5.64% goat, 1.85% sheep, 1.06% buffaloes, and 0.97% cattle were positive for Coxiella burnetii antibodies by enzyme linked immunosorbent assay kit (IDEXX, Liebefeld, Switzerland). In this preliminary study, we have proceeded to look for C. burnetii DNA in those antibody positive specimens employing an imported commercial C. burnetii polymerase chain reaction (PCR) kit.

Materials and Methods: Blood samples were collected during slaughtering. All 15 blood samples of antibody positive ruminants and three antibody negative samples were subjected to conventional Trans-PCR assay with a commercial PCR kit (Genekam Biotechnology AG, Duisburg, Germany). An in-house Trans-PCR was included in the study for comparison.

Results: A total of 15 antibody positive and three antibody-negative serum samples belonging to 11 goat, 4 sheep, 1 cattle, and 2 buffaloes were tested in duplicate for the presence of C. burnetii DNA by the commercial agar gel PCR kit and an in-house Trans-PCR. Only one buffalo serum sample was positive for C. burnetii with a band at 243 bp in in-house Trans-PCR.

Discussion: Seropositivity for C. burnetii need not necessarily translate into infectivity status of the animal. Conversely, seronegative ruminants can shed C. burnetii. Rapid disintegration of C. burnetii DNA during the storage period is an important impediment in QF-PCR research. This is the first time the performance of this commercial PCR kit is being validated in India.

Conclusion: Commercial PCR kit, Genekam did not identify any positive sample, probably because it targeted a larger amplicon of 687 bp.

Keywords: Coxiella burnetii DNA, coxiellosis, Trans-polymerase chain reaction.

Changes in histopathology and cytokeratin AE1/AE3 expression in skin graft with different time on Indonesian local cats

Research (Published online: 18-06-2017)

15. Changes in histopathology and cytokeratin AE1/AE3 expression in skin graft with 

different time on Indonesian local cats

Erwin, Etriwati, Gunanti, Ekowati Handharyani and Deni Noviana

Veterinary World, 10(6): 662-666

ABSTRACT

Aim: A good skin graft histopathology is followed by formation of hair follicle, sweat gland, sebaceous gland, blood vessel, lightly dense connective tissue, epidermis, and dermis layer. This research aimed to observe histopathology feature and cytokeratin AE1/AE3 expression on cat skin post skin grafting within a different period of time.

Materials and Methods: Nine male Indonesian local cats aged 1-2 years old weighing 3-4 kg were separated into three groups. First surgery created defect wound of 2 cm x 2 cm in size to whole groups. The wounds were left alone for several days, differing in interval between each group, respectively: Group I (for 2 days), Group II (for 4 days), and Group III (for 6 days). The second surgery was done to each group which harvested skin of thoracic area and applied it on recipient wound bed. On day 24th post skin graft was an examination of histopathology and cytokeratin AE1/AE3 immunohistochemistry.

Results: Group I donor skin's epidermis layer had not formed completely whereas epidermis of donor skin of Groups II and III had completely formed. In all group hair follicle, sweat gland, sebaceous gland, and neovascularization were found. The density of connective tissue in Group I was very solid than other groups. Cytokeratin AE1/AE3 expression was found on donor skin's epithelial cell in epidermis and dermis layer with very brown intensity for Group II, brown intensity for Group II, and lightly brown for Group I.

Conclusion: Histopathological structure and cytokeratin AE1/AE3 expression post skin graft are better in Groups II and III compared to Group I.

Keywords: cats, cytokeratin AE1/AE3, histopathology, skin graft.

Hemogram responses in goats toward challenged with Corynebacterium pseudotuberculosis and its immunogen mycolic acids

14. Hemogram responses in goats toward challenged with Corynebacterium pseudotuberculosis and its immunogen mycolic acids

Effect of season on physiological, biochemical, hormonal, and oxidative stress parameters of indigenous sheep

Research (Published online: 16-06-2017)

13. Effect of season on physiological, biochemical, hormonal, and oxidative stress parameters of indigenous sheep

Sawankumar D. Rathwa, A. A. Vasava, M. M. Pathan, S. P. Madhira, Y. G. Patel and A. M. Pande

Veterinary World, 10(6): 650-654

ABSTRACT

Aim: This study was conducted to evaluate the impact of summer and winter season on physiological, biochemical, hormonal, and antioxidant parameters in Indigenous sheep.

Materials and Methods: The research was carried out during summer and winter season. 8 adult apparently healthy female sheep (aged 2-4 years) of similar physiological status were selected. Daily ambient temperature and relative humidity were recorded to calculate the temperature-humidity index (THI). The THI value of summer and winter season were 82.55 and 59.36, respectively, which indicate extreme hot condition during summer season and extreme cold condition during winter season. Physiological parameters were recorded daily during the experimental periods. Blood samples were collected at weekly interval and analyzed for biochemical, hormonal, and antioxidant parameters. The results were analyzed using completely randomized design.

Results: From data obtained in this study, we found that higher THI during summer have significant effect over various physiological, biochemical, hormonal, and enzymatic indices of indigenous sheep. The physiological response such as rectal temperature, respiration rate (RR), pulse rate (PR), and skin temperature (ST) was increased significantly. We also found a significant increase in some biochemical parameters such as blood urea nitrogen (BUN), uric acid, creatinine (Cr), alanine transaminase (ALT), aspartate transaminase (AST), sodium (Na), and potassium (K). The level of cortisol hormone and superoxide dismutase (SOD), glutathione peroxidase (GPx), and lipid peroxidase (LPO) antioxidants increased significantly during summer. Whereas, some parameters such as glucose, cholesterol, calcium (Ca), inorganic phosphorus (IP), triiodothyronine (T3), and thyroxine (T4) were decreased significantly during summer season.

Conclusion: It was concluded that the THI is a sensitive indicator of heat stress and is impacted by ambient temperature more than the relative humidity in Indigenous sheep. Higher THI is associated with significant increase in RT, RR, PR, ST, BUN, uric acid, Cr, ALT, AST, Na, K, cortisol, SOD, GPx, and LPO and with a significant decrease in glucose, cholesterol, Ca, IP, T3 and T4.

Keywords: biochemical, indigenous sheep, physiological, temperature humidity index.

Induced pluripotent stem cell: A headway in reprogramming with promising approach in regenerative biology

Review (Published online: 14-06-2017)

12. Induced pluripotent stem cell: A headway in reprogramming with promising approach in regenerative biology

N. Rawat and M. K. Singh

Veterinary World, 10(6): 640-649

ABSTRACT

Since the embryonic stem cells have knocked the doorsteps, they have proved themselves in the field of science, research, and medicines, but the hovered restrictions confine their application in human welfare. Alternate approaches used to reprogram the cells to the pluripotent state were not up to par, but the innovation of induced pluripotent stem cells (iPSCs) paved a new hope for the researchers. Soon after the discovery, iPSCs technology is undergoing renaissance day by day, i.e., from the use of genetic material to recombinant proteins and now only chemicals are employed to convert somatic cells to iPSCs. Thus, this technique is moving straightforward and productive at an astonishing pace. Here, we provide a brief introduction to iPSCs, the mechanism and methods for their generation, their prevailing and prospective applications and the future opportunities that can be expected from them.

Keywords: cellular reprogramming, embryonic stem cells, induced pluripotent stem cells, stem cells.

Seroprevalence of antibodies to astrovirus in chickens in Grenada, West Indies

Research (Published online: 13-06-2017)

11. Seroprevalence of antibodies to astrovirus in chickens in Grenada, West Indies

Ravindra Nath Sharma, Romane Dufayet, Thomas Maufras, Kathryn O' Connell and Keshaw Tiwari

Veterinary World, 10(6): 636-639

ABSTRACT

Aim: Chicken astroviruses (CAstV) are known to cause mild gastroenteritis, growth depression, and even mortality in poultry, especially in chickens, turkeys, and ducks. To the best our knowledge, there is no published information on CAstV in Grenada. This study was conducted to determine the prevalence of astrovirus in chickens in Grenada.

Materials and Methods: Blood samples from 366 indigenous chickens and 92 commercial chicken layers were collected from all parishes of the island and tested for antibodies against CAstV using commercial enzyme-linked immunosorbent assay.

Results: The seroprevalence of antibodies against astrovirus was 57.6% (95%, Confidence interval [CI]: 47.4-67.2) in commercial layers and 61.5% (95%, CI: 56.4-66.3) in indigenous chickens. The results show the presence of infection throughout the island.

Conclusion: The results show the infection with CAstV in approximately half of the chicken population in Grenada. This is the first report on the prevalence of CAstV in chickens in Grenada and the Caribbean region.

Keywords: Astrovirus, chicken astroviruses, chickens, Grenada, seroprevalence.

Enhancing the growth performance of replacement female breeder goats through modification of feeding program

Research (Published online: 11-06-2017)

10. Enhancing the growth performance of replacement female breeder goats through modification 

of feeding program

A. A. A. Ghani, M. S. Shahudin, M. Zamri-Saad, A. B. Zuki, H. Wahid, A. Kasim, M. S. Salisi and 

Hasliza Abu Hassim

Veterinary World, 10(6): 630-635

ABSTRACT

Aim: The study was conducted at a smallholder goat farm located in Labu, Negeri Sembilan, Malaysia. The objective of this study was to evaluate the effect of proper feeding program on growth performances of replacement breeder goats.

Materials and Methods: A total of 30 healthy female boer cross goats at the age of 4 months old with average initial live body weight (BW) of 20.05±0.5 kg were used for on-farm feeding trial to evaluate the growth performance as preparation for breeding purposes. The experimental goats were divided into two groups of 15 animals each labeled as control and treatment groups, which were kept under intensive farming system. Goats in control group were fed with normal routine feeding protocol practiced by the farmer, while goats in the treatment group were fed with new feed formulation. Throughout the experimental period, on-farm monitoring and data collection were carried out. Initial BW and body condition score (BCS) were recorded before the start of the experiment while final BW and BCS were gained after 7 months of the experimental period. Average daily gain (ADG) was calculated after the experiment end. Data on BW, ADG, and BCS were recorded from both groups for every 2 weeks and reported monthly. The feed intake for the control group was 2.8 kg/animal/day which practiced by the farmer and 3.2 kg/animal/day as new feed formulation for the treatment group.

Results: After 7 months of the experimental period, final BW shows an improvement in treatment group (39.1±1.53 kg) compared with control group (32.3±1.23 kg). The ADG in treatment group also gives promising result when comparing with control group. Goats in treatment group significantly attained better ADG than control group which were 126.7 g/day and 83.3 g/day, respectively. For the BCS, goats in the treatment group had shown an improvement where 86.67% (13 out of 15) of the group had BCS≥3 (1-5 scoring scale) and only 66.67% (10 out of 15) of the control group had BCS≥3.

Conclusion: Therefore, it was concluded that implementation of proper feeding program as shown in treatment group give promising result to improve the growth performance of replacement breeder goats which can be adopted by the farmers to improve farm productivity.

Keywords: feeding, goat, growth performance, replacement breeder.

Study of morphology, chemical, and amino acid composition of red deer meat

Research (Published online: 10-06-2017)

9. Study of morphology, chemical, and amino acid composition of red deer meat

Eleonora Okuskhanova, Bahytkul Assenova, Maksim Rebezov, Kumarbek Amirkhanov, Zhanibek Yessimbekov, 

Farida Smolnikova, Almagul Nurgazezova, Gulnur Nurymkhan and Marilyne Stuart

Veterinary World, 10(6): 623-629

Aim: The aim of this study was to evaluate red deer (maral) meat quality based on chemical composition, pH, water-binding capacity (WBC), and amino acid content.

Materials and Methods: Maral meat surface morphology measurements were obtained by scanning electron microscopy. Active acidity (pH) was determined by potentiometry. Samples were analyzed for WBC by exudation of moisture to a filter paper by the application of pressure. Chemical composition (moisture, protein, fat, and ash fractions) was obtained by drying at 150°C and by extraction, using ethylic ether, and ashing at 500-600°C. The amino acid composition was obtained by liquid chromatography.

Results: Maral meat, with a pH of 5.85 and an average moisture content of 76.82%, was found to be low in fat (2.26%). Its protein content was 18.71% while its ash content was 2.21%. The amino acid composition showed that lysine (9.85 g/100 g), threonine (5.38 g/100 g), and valine (5.84 g/100 g) predominated in maral meat, while phenylalanine (4.08 g/100 g), methionine (3.29 g/100 g), and tryptophan (0.94 g/100 g) were relatively low in maral meat compared to other meats. The average WBC was found to be 65.82% and WBC was found to inversely correlate with moisture content.

Conclusion: Low-fat content, high mineral content, and balanced amino-acid composition qualify maral meat as a worthy dietary and functional food.

Keywords: amino acid, composition, maral, red deer meat, quality, water-binding capacity.

Effect of high and low roughage total mixed ration diets on rumen metabolites and enzymatic profiles in crossbred cattle and buffaloes

Research (Published online: 09-06-2017)