Hypoglycemic and hypolipidemic activity of combined milk thistle and fenugreek seeds in alloxan-induced diabetic albino rats

Research (Published online: 29-08-2020)

35. Hypoglycemic and hypolipidemic activity of combined milk thistle and fenugreek seeds in alloxan-induced diabetic albino rats

Mohamed Jamal Saadh

Veterinary World, 13(8): 1732-1736

ABSTRACT

Background and Aim: Despite the availability of antidiabetic drugs, they are not free from associated adverse side effects. This study aimed to evaluate the hypoglycemic and hypolipidemic effects of oral administration of seeds from two medicinal plants: (1) Milk thistle and (2) fenugreek.

Materials and Methods: Plant seeds were washed in distilled water and ground with a coffee grinder. Alloxan was used to induce diabetes in 20 male albino rats. Diabetic rats were randomly divided into two groups: (1) Group 1 (n=10), diabetic rats fed with 0.5 g/kg milk thistle and 2 g/kg fenugreek seeds per day and (2) Group 2 (n=10), diabetic rats fed standard rodent food for 4 weeks.

Results: Oral administration of milk thistle and fenugreek seeds for 2 weeks resulted in significant improvement in body weight, blood glucose, glycosylated hemoglobin (HbA1c), cholesterol, and triglyceride levels in alloxan-induced diabetic rats. After 4 weeks, this ameliorative effect was significantly elevated with respect to blood glucose (155.00±9.70 mg/ dL vs. 427.50±5.70 mg/dL; p<0.001), HbA1c (5.5±0.19% vs. 13.65±1.77%; p<0.001), cholesterol (281.50±10.95 mg/dL vs. 334.30±6.80 mg/dL; p<0.001), triglyceride (239.60±6.87 mg/dL vs. 284.20±9.95 mg/dL; p<0.01), and body weight (265.30±8.10 g vs. 207.40±11.4 g; p<0.01) as compared with non-treated diabetic rats.

Conclusion: Milk thistle and fenugreek seeds possess hypoglycemic and hypolipidemic properties and could be used as natural compounds that are suitable as parent compounds for the development of new antidiabetic drugs.

Keywords: fenugreek seeds, hypoglycemic activity, hypolipidemic activity, milk thistle seeds.

Prevalence of bovine tuberculosis in slaughtered cattle and factors associated with risk of disease transmission among cattle handlers at Oko-Oba Abattoir, Lagos, Nigeria

Research (Published online: 29-08-2020)

34. Prevalence of bovine tuberculosis in slaughtered cattle and factors associated with risk of disease transmission among cattle handlers at Oko-Oba Abattoir, Lagos, Nigeria

Musiliu Abiodun Agbalaya, Olayinka Olabisi Ishola, Hezekiah Kehinde Adesokan and Olufunmilayo Ibitola Fawole

Veterinary World, 13(8): 1725-1731

ABSTRACT

Background and Aim: Bovine tuberculosis (bTB) is a zoonotic disease of major public health importance, especially in many developing countries, including Nigeria, where control measures are largely not applied, and the risks of human infection are high. This study was aimed at determining the current prevalence of bTB in slaughtered cattle and identifying factors associated with the risk of disease transmission among cattle handlers toward making informed control measures to limit human-animal interface disease transmission.

Materials and Methods: Serum samples at slaughter and lesions suggestive of bTB collected during postmortem examination of 187 slaughtered cattle at the Oko–Oba Abattoir, Agege, Lagos State, Nigeria, were subjected to lateral flow and Ziehl–Neelsen (ZN) techniques, respectively. Furthermore, a structured questionnaire was interviewer-administered to 156 cattle handlers to investigate associated exposure factors to bTB infection. Data were analyzed using bivariate and multivariate logistic regression.

Results: The prevalence of bTB in cattle was 25.7% and 7.0% by lateral flow technique and ZN, respectively. The seropositivity was highest in cattle with poor body condition (50.0%), then with good (36.4%) and fair (25.0%) body conditions. The questionnaire survey revealed that being in livestock handling business for >6 years (p=0.001), not knowing the mode of transmission (p=0.02) and ignoring TB lesions at slaughter (p=0.02) were exposure factors associated with increased risk of bTB infection among the cattle handlers. Further, multivariate analysis showed that those who spent more than 6 years in livestock handling were about 4 times (Adjusted odds ratio [AOR]=3.5; 95% confidence interval [CI]=1.1-7.6, p=0.01) more likely to be exposed to bTB infection than those with lesser years. Again, respondents who called the attention of meat inspectors on seeing lesions in animals were about 4 times less likely to be exposed to bTB infection than those who ignored it (AOR=0.3; CI=0.1-0.8, p=0.01).

Conclusion: This study has reiterated the endemicity of bTB in cattle population in Nigeria, with the prevalence of 25.7% and 7.0% of bTB by lateral flow and ZN techniques, respectively. This portends potential risk for disease transmission at the human-animal interface, particularly at the abattoir setting. The study also identified important knowledge and practice gaps which would enable informed, all-inclusive, and well-directed programs for effective control of the disease in both human and cattle populations.

Keywords: abattoir, bovine tuberculosis, cattle handlers, prevalence, public health.

Humoral responses to Schistosoma japonicum soluble egg antigens in domestic animals in Lindu Subdistrict, Central Sulawesi Province, Indonesia

Research (Published online: 29-08-2020)

2. Humoral responses to Schistosoma japonicum soluble egg antigens in domestic animals in Lindu Subdistrict, Central Sulawesi Province, Indonesia

Novericko Ginger Budiono, Sri Murtini, Fadjar Satrija, Yusuf Ridwan and Ekowati Handharyani

International Journal of One Health, 6(2): 99-108

ABSTRACT

Background and Aim: Schistosomiasis japonica, a disease caused by Schistosoma japonicum, is a public health problem in the Philippines, the Republic of Indonesia, and the People's Republic of China. The disease is known as zoonotic, meaning other than humans, animals are involved as the reservoirs. In Indonesia, schistosomiasis surveillance in animals is not continuous. Thus, the study to determine the prevalence of the disease in animals is needed. The study was aimed to determine the seroprevalence of S. japonicum infection among four species of domestic animals in the Lindu Sub-district, Central Sulawesi Province of Indonesia.

Materials and Methods: Blood samples of domestic animals were collected and analyzed for the presence of anti-S. japonicum immunoglobulin G antibodies against S. japonicum soluble egg antigens using the indirect hemagglutination assay. Animal stool samples were collected, and the miracidia-hatching assay was used for the detection of S. japonicum infection. Additional data concerning the animal identity and the management practices were obtained through a questionnaire used in surveys and interviews.

Results: A total of 146 sera from 13 cattle, 24 buffaloes, 54 pigs, and 55 dogs were collected. The overall schistosomiasis seroprevalence was 64.4%. The serology prevalence in cattle, buffalo, pig, and dog was 100.0%, 41.7%, 74.1%, and 56.4%, respectively. Domestic animals in all of five villages have previous exposure with S. japonicum as seropositive animals detected in every village. A total of 104 animal stool samples from 146 animals sampled were obtained. The overall schistosomiasis prevalence determined by the miracidia hatching assay was 16.35%. The sensitivity and specificity of indirect hemagglutination assay (IHA) in the current study were 88.24% and 41.37%, respectively, with miracidia hatching assay as the gold-standard method.

Conclusion: This study has shown a high seroprevalence of schistosomiasis japonica among domestic animals in the Lindu Subdistrict. IHA can be used as the screening method for the detection of S. japonicum infection in domestic animals. Chemotherapy and animal livestock grazing management programs to reduce the parasite burden and Schistosoma egg contamination in the environment must be implemented as part of one health approaches, in addition to other control measures.

Keywords: Indonesia, one health, schistosomiasis japonica, seroprevalence, zoonotic parasitic disease.

Development of a coagglutination kit as a rapid test for diagnosing Newcastle disease in poultry

Research (Published online: 28-08-2020)

33. Development of a coagglutination kit as a rapid test for diagnosing Newcastle disease in poultry

Muhammad Kholish Naf'an, Kurniasih Kurniasih, Tri Untari and Yos Adi Prakoso

Veterinary World, 13(8): 1719-1724

ABSTRACT

Background and Aim: Newcastle disease (ND) is a viral infection that causes high mortality and economic loss in the poultry industry. The Office International des Epizooties (OIE) recommends several diagnostic methods for the detection of ND, including isolation and molecular tests. However, these detection methods are time-consuming and highly expensive. Therefore, this study was conducted to develop a coagglutination kit as a novel diagnostic tool for ND in the poultry industry.

Materials and Methods: Two adult male New Zealand White rabbits weighing 2.5 kg were vaccinated using ND life vaccine intraperitoneally. The vaccination was conducted once a week for 4 weeks with multilevel doses. Rabbits' serum was collected at week 6 and inactivated at 56°C for 30 min. The serum was precipitated using ammonium sulfate and reacted with protein A of Staphylococcus aureus to produce the agglutination kit for detecting ND virus. A total of 25 chickens suspected with ND infection from a local poultry farm in Yogyakarta were used as the test samples. The chickens were necropsied, and the brain, spleen, lung, intestine, and feces were collected. Half of these organs were subjected to tests using the coagglutination kit and reverse transcription-polymerase chain reaction (RT-PCR). The other half was processed for histopathology. Data were analyzed qualitatively.

Results: Of the 25 samples, 13 (52%) were positive for ND infection when tested using both the ND coagglutination kit and RT-PCR. The positive samples also exhibited several histopathological changes, including perivascular cuffing surrounding the cerebral blood-brain barrier, hemorrhagic pneumonia, splenitis, and necrotic hemorrhage enteritis.

Conclusion: This study confirmed that the ND coagglutination kit could be used as a novel diagnostic tool for the detection of ND virus infection in the poultry industry.

Keywords: coagglutination kit, histopatology, Newcastle disease, rapid test, reverse transcription-polymerase chain reaction.

Genetic and non-genetic factors affecting semen production and quality characteristics of Gir cattle breed under semi-arid climate

Research (Published online: 27-08-2020)

32. Genetic and non-genetic factors affecting semen production and quality characteristics of Gir cattle breed under semi-arid climate

K. G. Bhave, K. Thilak Pon Jawahar, P. Kumarasamy, T. Sivakumar, C. Joseph, T. Shirsath, P. Deshmukh and R. Venkataramanan

Veterinary World, 13(8): 1714-1718

ABSTRACT

Aim: This study aims to evaluate genetic and non-genetic factors influencing semen production potential of Gir bulls.

Materials and Methods: Data on semen quantity (n=6911) and quality (n=466) available from January 2011 to December 2018 at BAIF's frozen semen station, Jind, Haryana, India, were utilized for the study. Factors, namely, season of collection, age at collection, and bull (random effect) were studied for their effect on quantitative and qualitative semen traits. Least square means for the traits were obtained using a general linear model. The effect of age within bull for repeatable traits was analyzed using a longitudinal model with age as the control variable. Multivariate analysis using mixed repeatability model equation was utilized to estimate bull effect correlation (genetic + permanent environmental correlation), phenotypic correlations, and repeatability.

Results: The overall least square means of ejaculate volume, sperm concentration, total sperms, initial and post-thaw motility, hypo-osmotic swelling test, and acrosome integrity of frozen semen were 6.62±0.03 ml, 1.22±0.01 109/ml, 8.09±0.05 109/ml ejaculate, 75.78±0.001%, 55.92±0.0001%, 55.13±0.005%, and 71.08±0.001%, respectively. The season of the collection showed a significant effect on volume, concentration, total sperm, and initial motility. The performance of bulls was superior in summer season, followed by winter and monsoon. Increase in semen attributes during summer season was due to the effect of lower temperature on sensitive stages of spermatogenesis. Age at collection had a significant effect on all semen traits. Volume and total sperm count showed increasing trend while concentration showed a decreasing trend with an increase in age. Motility and quality traits did not show any particular pattern. Individual bulls showed differences in all the semen performance traits with age. The repeatability of the traits ranged from 0.04 (HOST) to 0.58 (acrosome integrity). Bull effect correlation ranged from –0.73 (initial motility and acrosome integrity) to 0.93 (HOST and acrosome integrity).

Conclusion: Individual bulls showed variation in traits measured over age. The result of the study could be utilized in suggesting suitable management plans to achieve the desired profit by improving semen quality in Gir bulls.

Keywords: Gir, longitudinal analysis, repeatability, bull effect, and semen production.

Relationship between somatic cell counts and subclinical mastitis in lactating dairy cows

Research (Published online: 27-08-2020)

31. Relationship between somatic cell counts and subclinical mastitis in lactating dairy cows

S. M. Mostafizur Rahaman Sumon, Mst. Sonia Parvin, Md. Amimul Ehsan and Md. Taohidul Islam

Veterinary World, 13(8): 1709-1713

ABSTRACT

Background and Aim: Subclinical mastitis (SCM) is one of the most prevalent diseases of dairy cows, and somatic cell count (SCC) is widely used to determine SCM and milk quality. However, setting the threshold of SCC is very important. This study aimed to determine the cow-level threshold of SCC to differentiate milk of SCM affected cows from normal milk in Bangladesh.

Materials and Methods: Sensitivity (Se) and specificity (Sp) along with other characteristics of different thresholds of SCC were determined considering the bacterial culture as the gold standard test. Three definitions of intramammary infection (IMI) were set based on the group of pathogens involved. Five categories of SCC thresholds were considered for analysis.

Results: Se and Sp of thresholds of SCC greatly varied as definitions of IMI changed. Irrespective of SCC thresholds, Se increased when isolation of major pathogens included in IMI definitions. Se decreased when SCC thresholds increased (from 100 to 300 × 103 cells/mL) for all IMI definitions and ranged from 60.6% to 20.3%. The highest Se was found at low SCC threshold (100 × 103 cells/mL), which resulted in less false-negative outcome. On the other hand, Sp increased with the increment of SCC thresholds giving rise to a less false-positive outcome.

Conclusion: The cow-level SCC threshold of 100 × 103 cells/mL was found appropriate to identify subclinical IMI of dairy cows.

Keywords: Bangladesh, bovine mastitis, intramammary infection, somatic cell count threshold, sensitivity, specificity.

Unique patterns of cardiogenic and fibrotic gene expression in rat cardiac fibroblasts

Research (Published online: 26-08-2020)

30. Unique patterns of cardiogenic and fibrotic gene expression in rat cardiac fibroblasts

Kittipong Tachampa and Tuempong Wongtawan

Veterinary World, 13(8): 1697-1708

ABSTRACT

Background and Aim: Cardiac fibroblasts are important for both normal and pathological states of the heart, but the knowledge in cell physiology and genomics is still poorly understood. The aims of the present study were; first, to investigate the expression of cardiac and fibrotic genes in rat cardiac fibroblasts compared to cardiomyocytes and other fibroblasts (skin and muscle fibroblasts), second, to examine the in vitro effect of serum concentration on fibroblast gene expression. The findings can potentially be applied in ischemia/reperfusion models.

Materials and Methods: Rat cardiac fibroblasts were collected and cultured in different conditions, and their gene expression (21 cardiogenic genes and 16 fibrotic genes) was compared with cardiomyocytes and other fibroblasts using comparative quantitative polymerase chain reaction. We also mimicked myocardial ischemia/reperfusion by depleting and then adding a serum into the culture in conventional culture (10% serum).

Results: Cardiac fibroblasts expressed most of the cardiogenic genes, but their expression levels were significantly lower than in cardiomyocytes, while almost all fibrotic genes in the cardiac fibroblasts were significantly more highly expressed than in cardiomyocytes, except matrix metallopeptidase 9 (Mmp9) which also had greater expression in other fibroblasts. After mimicking cardiac ischemia and reperfusion in vitro by starving and then adding a serum into the cardiac fibroblast culture, the results revealed that Mmp9 expression was significantly increased (>30 times) after increasing but not reducing the serum in the culture. The expression of most cardiogenic and fibrotic genes in cardiac fibroblasts tended to decrease after increasing the serum in the culture. These changes were specific to cardiac fibroblasts but no other fibroblasts.

Conclusion: Cardiac fibroblasts have a distinct pattern of gene expression from other fibroblasts and cardiomyocytes. They are also sensitive to high serum concentration but not affected by serum depletion, suggesting that the process of developing cardiac fibrosis might be stimulated by reperfusion or overcirculation rather than ischemia. The cell starvation followed the adding of serum may serve as a useful model to study cardiac fibrosis cause by the change of blood flow.

Keywords: cardiac fibroblasts, cardiomyocytes, gene expression, in vitro model, ischemia, reperfusion.

Applications, challenges, and strategies in the use of nanoparticles as feed additives in equine nutrition

Review (Published online: 26-08-2020)

29. Applications, challenges, and strategies in the use of nanoparticles as feed additives in equine nutrition

P. Ravi Kanth Reddy, Duvvuru Yasaswini, P. Pandu Ranga Reddy, Mohamed Zeineldin, M. J. Adegbeye and Iqbal Hyder

Veterinary World, 13(8): 1685-1696

ABSTRACT

The rapid expansion of nanotechnology has been transforming the food industry by increasing market share and expenditure. Although nanotechnology offers promising benefits as feed additives, their usage in equines is primarily geared toward immunotherapy, hyper-immunization techniques, drug delivery systems, grooming activities, and therapeutic purposes. Nanoparticles could be engaged as alternatives for antibiotic feed additives to prevent foal diarrhea. Gold nanoparticles are proved to provide beneficial effects for racehorses by healing joint and tendon injuries. Because of the poor bioavailability of micro-sized mineral salts, the usage of nano-minerals is highly encourageable to improve the performance of racehorses. Nano-Vitamin E and enzyme CoQ10 for equines are no longer a simple research topic because of the increased commercial availability. Employing nanotechnology-based preservatives may offer a promising alternative to other conventional preservatives in preserving the quality of equine feed items, even during an extended storage period. While nanoparticles as feed additives may provide multitudinous benefits on equines, they could elicit allergic or toxic responses in case of improper synthesis aids or inappropriate dosages. The safety of nano-feed additives remains uninvestigated and necessitates the additional risk assessment, especially during their usage for a prolonged period. To adopt nano-feed additives in horses, there is an extreme paucity of information regarding the validity of various levels or forms of nanoparticles. Further, the currently available toxicological database on the topic of nano-feed additives is not at all related to equines and even inadequate for other livestock species. This review aims to provide new insights into possible future research pertaining to the usage of nano-feed additives in equines.

Keywords: antimicrobial, drug delivery, equine nutrition, feed additives, nano-minerals, nanoparticles.

Isolation and characterization of Clostridium perfringens strains isolated from ostriches (Struthio camelus) in Vietnam

Research (Published online: 25-08-2020)

28. Isolation and characterization of Clostridium perfringens strains isolated from ostriches (Struthio camelus) in Vietnam

Tham Thi Nguyen, Hung Vu-Khac and Tan Duc Nguyen

Veterinary World, 13(8): 1679-1684

ABSTRACT

Background and Aim: Clostridium perfringens can cause enteritis in ostriches. The toxin release is believed to play a major role in determining pathogenesis properties of these pathogenic bacteria. This study was conducted to isolate and characterize C. perfringens strains from ostriches in Vietnam for identifying if particular virulence factors of these pathogenic bacteria are associated with enteritis progress in ostriches.

Materials and Methods: The prevalence of cpa, cpb, iA, etx, cpe, and cpb2 genes among C. perfringens isolates was determined by a multiplex polymerase chain reaction (PCR) method. The NetB toxin-encoding gene was detected by PCR and then sequenced to observe their variation. The expression of NetB toxin was checked by SDS-PAGE.

Results: A total of 116 C. perfringens isolates were obtained from 318 fecal samples and 105 intestinal organs. Of 80 isolates from fecal samples, 33 isolates were from healthy and 47 isolates were from diseased ostriches. The results of multiplex PCR showed that all 116 C. perfringens strains from healthy and enteric disordered ostriches were positive for the alpha toxin-encoding gene (cpa). The cpe and cpb2 genes were found in only one and five diseased ostriches, respectively. The netB gene was detected in 1/33 (3.03%) C. perfringens isolates from healthy ostriches, in 8/47 (17.05%) isolates from feces, and in 7/36 (19.44%) intestinal contents of diseased ostriches. The full-length sequences of 5 out of 15 netB-positive isolates from diseased ostriches showed 100% identity to each other as well as to the netB sequences available in GenBank. All of these five isolates produced NetB toxin in vitro.

Conclusion: Type A is the most prevalent among C. perfringens isolates from ostriches in Vietnam. Especially, the study provides data emphasizing the role of NetB toxin in causing necrotic enteritis by C. perfringens in ostriches.

Keywords: Clostridium perfringens, multiplex polymerase chain reaction, ostriches, toxin genes.

Lassa fever: History, causes, effects, and reduction strategies

Review (Published online: 25-08-2020)

1. Lassa fever: History, causes, effects, and reduction strategies

Promise Tewogbola and Norah Aung

International Journal of One Health, 6(2): 95-98

ABSTRACT

Lassa fever is a disease that is not well-known worldwide, particularly due to the inability of the multimammate rat, the primary vector of the Lassa virus, to breed in temperate regions. The aim of this review is to provide an overview of the disease and its modus operandi while also providing information about trends in the past decade, as well as proven strategies that have been used to manage its spread.

Keywords: Arenavirus, epidemic, epidemiology, Lassa fever, Lassa virus, virus.

Investigation of Trypanosoma evansi infection in bullfighting cattle in Southern Thailand

Research (Published online: 22-08-2020)

27. Investigation of Trypanosoma evansi infection in bullfighting cattle in Southern Thailand

Ketsarin Kamyingkird, Piangjai Chalermwong, Vannarat Saechan, Domechai Kaewnoi, Marc Desquesnes and Ruttayaporn Ngasaman

Veterinary World, 13(8): 1674-1678

ABSTRACT

Background and Aim: Trypanosoma evansi infection has been reported in Thai livestock such as beef and dairy cattle. However, there is little information on T. evansi infection in bullfighting cattle in Southern Thailand. The aim of this study was to investigate the infection of T. evansi in bullfighting cattle presented for health checks at the Animal Hospital, Faculty of Veterinary Science, Prince of Songkla University, Thailand.

Materials and Methods: Blood and serum samples were collected from 177 bullfighting cattle from April 2016 to February 2017 after bullfighting matches. Animal inspected showed signs of fever, weight loss, or exercise intolerance. Investigation of T. evansi infection was tested using polymerase chain reaction (PCR) with TBR primers and using indirect enzyme-linked immunosorbent assay with T. evansi crude antigen.

Results: The seroprevalence of T. evansi in bullfighting cattle was 22.60% (40/177). The PCR results detected no parasite DNA in this study. However, bullfighting cattle may serve as T. evansi reservoirs.

Conclusion: Health checking procedures for T. evansi should be promoted for bullfighting events so that infected animals can be quarantined in the preparatory stages of such events.

Keywords: bullfighting cattle, seroprevalence, Thailand, Trypanosoma evansi.

Expressions of miR-155 and miR-181 and predictions of their structures and targets in pigs (Sus scrofa)

Research (Published online: 21-08-2020)

26. Expressions of miR-155 and miR-181 and predictions of their structures and targets in pigs (Sus scrofa)

Jirapat Ninsuwon, Pitchaporn Waiyamitra, Atthaporn Roongsitthichai and Win Surachetpong

Veterinary World, 13(8): 1667-1673

ABSTRACT

Background and Aim: MicroRNAs (miRNAs) are responsible for gene expression control at the post-transcription level in many species. Several miRNAs are required in the regulation of immune responses, such as B-cell differentiation, T-cell receptor signaling pathway, CD4+ T cell selection, and so on. Studies on miRNAs have been extensively conducted in humans and mice; however, reports relevant to miRNAs, especially miR-155 and miR-181, in pigs are limited. Consequently, the present study aimed to investigate the structures, target genes, and expressions of miR-155 and miR-181 in various porcine cells and tissues.

Materials and Methods: Five healthy male pigs from a porcine reproductive and respiratory syndrome virus-negative farm were studied. Before slaughter, blood samples were collected for peripheral blood mononuclear cell isolation. After slaughter, samples of spleen, lymph nodes, and forelimb muscles were collected. Both miR-155 and miR-181 were investigated for their structures with RNAfold web server, for their target genes from three online web servers, and for their expressions using polymerase chain reaction (PCR).

Results: The structures of miR-155 and miR-181 contained hairpins with free energies of –35.27 and –35.29 kcal/mole, respectively. Target gene prediction revealed that miR-155 had perfect complementarity with Socs1 and Mapk3k14, while miR-181 had perfect complementarity with Ddx3x, Nfat5, Foxp1, and Mpp5. PCR showed that both miRNAs were detectable from all investigated cells and tissues. Moreover, the highest expression of both miRNAs was found from the lymph node of the pigs.

Conclusion: Both miR-155 and miR-181 might be involved with the regulation of porcine immune functions as both miRNAs were detected in several cells and tissues of the pigs. In addition, they had very high complementarities with the seed regions of several immune-related genes.

Keywords: immune system, microRNA, miR-155, miR-181, porcine.

Field serological investigation for peste des petits ruminants, foot-and-mouth disease, and bluetongue diseases in illegally introduced animals in Egypt

Research (Published online: 21-08-2020)

25. Field serological investigation for peste des petits ruminants, foot-and-mouth disease, and bluetongue diseases in illegally introduced animals in Egypt

Wafaa Abd El Wahab Hosny, Eman Mohamed Baheeg, Hala Abd El Raheem Aly, Samia Said Abd El Nabi and Nadia Maher Hanna

Veterinary World, 13(8): 1661-1666

ABSTRACT

Aim: In this study, laboratory scoping on the viruses that cause peste des petits ruminants (PPR), bluetongue (BT), and foot-and-mouth disease (FMD) was performed to evaluate the current status of animals illegally introduced into Egypt. This study aims to help control these infectious illnesses and tries to prevent the introduction of other strains of these three viruses to Egypt, as these illnesses spread quickly if not controlled.

Materials and Methods: In the year 2018, 62 serum samples were collected and serologically tested through competitive enzyme-linked immunosorbent assays (ELISA) kits to detect antibodies against PPR, BT, and FMD, which are three important transboundary infectious illnesses.

Results: The results indicated that 60 out of 62 serum samples were positive for PPR antibodies (96.7%), 31 out of 62 were positive for FMD antibodies (50%), and 59 out of 62 serum samples were positive for BT antibodies (95%).

Conclusion: This study revealed that PPR, FMD, and BT can be introduced into Egypt through the illegal introduction of sheep and goat from neighboring countries. Laboratory diagnostic abilities should be improved for the early detection and control of these illnesses.

Keywords: bluetongue and foot-and-mouth disease sheep-goats, competitive enzyme-linked immunosorbent assays, peste des petits ruminants.

The effect of the sex, age, and breed of farmed rabbits and the choice of management system on the extensity and intensity of Eimeria infection

Research (Published online: 20-08-2020)

24. The effect of the sex, age, and breed of farmed rabbits and the choice of management system on the extensity and intensity of Eimeria infection

B. Pilarczyk, A. Tomza-Marciniak, R. Pilarczyk, E. Januś, P. Stanek, B. Seremak and P. Sablik

Veterinary World, 13(8): 1654-1660

ABSTRACT

Background and Aim: The most common causes of loss and diarrhea in rabbit farming are nutritional errors and coccidiosis. The infection can spread rapidly throughout a breeding area, reducing the rabbit population, and causing heavy losses. The aim of the study was to determine the influence of the system of animal management on the extensity and intensity of infection by Eimeria of farmed rabbits, together with the effect of the sex, age, and breed of the rabbits themselves.

Materials and Methods: The study included 91 rabbits (Flemish Giant, New Zealand White, French Lope, Vienna Blue, California White, and mixed breed) from eight domestic (small-scale) farms from Poland. The prevalence and intensity of coccidial infection were determined by the Willis-Schlaf and McMaster coprological methods. The species were determined based on oocyst morphology: Their shape, color, form index, the presence or absence of micropyle and cap, and the presence or absence of residual, polar, and Stiedé bodies.

Results: Seven species of Eimeria were isolated from the tested rabbits: Eimeria magna, Eimeria media, Eimeria perforans, Eimeria stiedae, Eimeria coecicola, Eimeria exigua, and Eimeria irresidua. Most infections were found to be of relatively low intensity. No significant differences in the extensity of Eimeria protozoan infection were observed with regard to sex. However, rabbit age had a significant influence on the extensity of infection by E. magna and of Eimerian protozoans combined. The greatest extensity was observed in rabbits aged below 6 months. For all species of Eimeria, greater extensity was observed among rabbits kept in groups than individually. The system of rabbit management also had a significant influence on the intensity of infection. Those kept in groups demonstrated a significantly higher mean intensity of infection of E. magna and all Eimeria species combined than those kept individually.

Conclusion: Our findings indicate that Eimeria protozoa are a common occurrence on small-scale rabbit farms. As coccidiosis treatment does not always give good results, prevention is very important in the fight against this disease. It is necessary to develop a new preventive paradigm that pays special attention to the factors that promote the spread and development of infection in domestic (small-scale) farms from Poland. For example, it would be recommended to use large, dry, bright rooms with access to the sun, as these are conducive to preventing the occurrence of coccidia infections.

Keywords: Eimeria, rabbits, small-scale rabbit farms.

Investigation of the effect of chitosan and silver nanoparticles on the antibiotic resistance of Escherichia coli O157:H7 isolated from some milk products and diarrheal patients in Sohag city, Egypt

Research (Published online: 20-08-2020)

23. Investigation of the effect of chitosan and silver nanoparticles on the antibiotic resistance of Escherichia coli O157:H7 isolated from some milk products and diarrheal patients in Sohag city, Egypt

Alshimaa A. Hassanien and Eman M. Shaker

Veterinary World, 13(8): 1647-1653

ABSTRACT

Background and Aim: Antimicrobial-resistant Escherichia coli O157:H7 causes serious diseases in humans, especially when circulated in their food. This study was designed to detect the presence of E. coli O157:H7 using the fliC H7 gene in some milk products as kareish cheese, labena, and yoghurt sold in Sohag city, Egypt, and among diarrheal patients admitted to governmental hospitals in Sohag and also to highlight the risk factors associated with their infection. In addition, the antimicrobial resistance and the effect of chitosan nanoparticles (CNP) and silver nanoparticles (SNP) on E. coli O157:H7 isolates obtained from both milk products and patients were investigated.

Materials and Methods: Microbiological culture methods and polymerase chain reaction were used for detecting E. coli O157:H7 in 150 milk products and 150 stool samples. Resistance against some antimicrobials that were used in the treatment of animals and humans was investigated using the disk diffusion technique. CNP and SNP at two concentrations (30 and 60 μg/mL) and average sizes of 25.1 and 26.5 nm, respectively, were identified by transmission electron microscopy. Their effect on E. coli O157:H7 isolates was examined using the well diffusion method. Risk factors for infection were investigated using statistical analysis.

Results: There were 11.3% and 14.7% of milk products and stool samples positive for E. coli O157:H7, respectively. These isolates exhibited high antimicrobial resistance to ampicillin, tetracycline, and gentamycin. CNP and SNP demonstrated inhibitory effects on E. coli O157:H7 growth, which significantly increased at high concentrations (60 μg/mL), with mean inhibition zones of 31.941±3.749 and 30.681±3.871 mm for CNP in milk products and patient isolates, respectively. The respective values for SNP were 33.588±3.675 mm and 32.500±2.444 mm, indicating a higher bactericidal effect than that of CNP. Regarding risk factors for infection, both young and elderly subjects and those in contact with infected persons and/or having chronic diseases were infected.

Conclusion: CNP and SNP are suitable for both medical and agricultural applications for disease control and enhancement of food quality.

Keywords: chitosan nanoparticles, diarrheal patients, Escherichia coli O157:H7, kariesh cheese, labena, silver nanoparticles.

Improvements to the live-attenuated Newcastle disease virus vaccine using Carbopol® 940 as a stabilizer

Research (Published online: 19-08-2020)

22. Improvements to the live-attenuated Newcastle disease virus vaccine using Carbopol® 940 as a stabilizer

Mahmoud Mohamed Abd El-Moneam, Nada Adel Fathy, Naglaa I. Ali and Heba Mohamed El Naggar

Veterinary World, 13(8): 1641-1646

ABSTRACT

Background and Aim: One strategy that can be used to stabilize vaccines is to convert them into a dry powder. This can protect the integrity of the active ingredients as well as vaccine antigenicity during manufacture, storage, and transport. This study highlights the potent adjuvant activity of Carbopol® when used alone to stabilize live-attenuated Newcastle disease virus (NDV) vaccines or when used in a formulation together with skimmed milk. Tolerability and potency of these formulations were compared with those obtained from other local live NDV vaccines produced locally by the Veterinary Serum and Vaccine Research Institute.

Materials and Methods: We evaluated the cellular and humoral immune responses to a locally prepared, live-attenuated LaSota virus vaccine. Vaccine formulations were stabilized with Carbopol® 940 alone or in combination with skimmed milk.

Results: Our results indicate that the use of Carbopol® 940 alone to stabilize a live-attenuated LaSota vaccine resulted in enhanced cellular and humoral immunity. The antibody titer was prolonged through the 6th week post-vaccination (5.0 log2). Full (100%) protection was observed in response to challenge with very virulent NDV at day 21 after vaccination; there were no clinical signs or lesions on examination. Addition of Carbopol® 940 to the live-attenuated vaccine formulation resulted in a more compact, stable, and high-quality lyophilized cake after freeze-dried lyophilization compared with that produced by stabilization with skimmed milk alone.

Conclusion: Our data suggest that Carbopol® 940 may improve clinical responses to live-attenuated vaccines.

Keywords: Carbopol® 940, LaSota, lyophilization, Newcastle disease virus, stabilizers, vaccine.

Gastrointestinal parasites of dromedary camel (Camelus dromedarius) in Algeria

Research (Published online: 19-08-2020)

21. Gastrointestinal parasites of dromedary camel (Camelus dromedarius) in Algeria

Messaoud Bouragba, AbdElkarim Laatamna, Fatima Elzahra Cheddad, Djamel Baroudi, Karim Houali and Ahcène Hakem

Veterinary World, 13(8): 1635-1640

ABSTRACT

Aim: The present study was designed to investigate the prevalence and identification of gastrointestinal parasites in feces samples of dromedary camels (Camelus dromedarius) in Algeria based on microscopic examination.

Materials and Methods: A total of 717 fresh fecal samples obtained from 28 farms at Steppe and Northern Sahara regions of Algeria were processed for microscopic examination after concentration by formalin-ether sedimentation and flotation techniques. In addition, microscopic examination of Cryptosporidium spp. was done by modified Ziehl-Neelsen staining and Lugol staining procedure was used for the detection of Giardia cysts.

Results: Microscopic examination indicated an infection rate of gastrointestinal parasites of 48.26% (346/717). Protozoan infections were recorded at 17.02% (122/717), whereas helminth infections were recorded at 23.71% (170/717). In addition, mixed infection (protozoans and helminths) was seen at 7.53% (54/717). No correlation was found between infection and age of the animals, nor the consistency of the stool samples; in addition, neither influence of sex nor breed of camels was observed. Eighteen genera of gastrointestinal parasites were revealed; including four genera of protozoa, 12 Nematoda, one Cestoda, and one Trematoda. Strongyloides spp. and Eimeria spp. showed the highest rate of parasitism, while Cooperia spp. was observed with the lowest prevalence. Cryptosporidium spp. was detected in 13 among 717 examined samples (1.81%).

Conclusion: The parasite fauna infecting the gastrointestinal tract of the Algerian dromedary is much diversified. The detected parasites in camels are similar to counterparts in other ruminants, posing serious challenge to animal farming. Future studies should be carried out to better understand the epidemiology of these parasitic diseases and their economic and public health impact.

Keywords: Algeria, camel dromedary, helminths, prevalence, protozoans.

Histological classification and expression of markers of canine mast cell tumors

Research (Published online: 18-08-2020)

20. Histological classification and expression of markers of canine mast cell tumors

V. S. Cruz, J. C. A. Borges, L. L. Nepomuceno, P. A. M. Gonçalves, Y. C. L. Prado, C. Bianchi, M. C. S. Fioravanti and E. G. Araújo

Veterinary World, 13(8): 1627-1634

ABSTRACT

Background and Aim: Mast cell tumors (MCTs) are malignant neoplasms that are common in dogs. Their biological behavior is variable and unpredictable. The aim of the present study was to analyze the histological classification and expression of markers of canine MCTs.

Materials and Methods: Thirty samples of canine MCTs were graded according to the histological classification methods of Patnaik and those of Kiupel. The expression of phosphoprotein 53 (p53) and c-kit proteins was quantified by immunohistochemistry using image processing software, ImageJ - a public domain computer program, developed at the National Institutes of Health.

Results: It was possible to determine the grade of 100% of the samples. According to Patnaik's classification, 20.00% of the samples were Grade 1, 43.30% were Grade 2, and 36.70% were Grade 3. According to Kiupel's classification, 56.67% of the samples were of high intensity and 43.33% were of low intensity. Grade 1 tumors had the highest expression of p53 and c-kit, and Grade 2 had the lowest expression. The results showed that it is necessary to perform both histological grading methods. The classification into high and low intensity may provide more consistent results than the three-level grading system. However, a smaller number of categories, although it facilitates the classification, may not be sufficient for the prognosis.

Conclusion: Quantitative evaluation of p-53 and c-kit expression is a useful tool to increase the accuracy of the analysis and to aid in choosing the treatment method for canine MCTs. Histological grading should be combined with other diagnostic methods.

Keywords: Bismarck brown, hematoxylin and eosin, ImageJ, round cell tumor, toluidine blue.

Monitoring of serum and urinary biomarkers during treatment of canine visceral leishmaniasis

Research (Published online: 18-08-2020)

19. Monitoring of serum and urinary biomarkers during treatment of canine visceral leishmaniasis

Alvaro Felipe de Lima Ruy Dias, Eveline da Cruz Boa Sorte Ayres, Fernanda Harumi Maruyama, Bruna Ribeiro Gomes Monteiro, Maria Sabrina de Freitas, Arleana do Bom Parto Ferreira de Almeida, Adriane Jorge Mendonça and Valéria Régia Franco Sousa

Veterinary World, 13(8): 1620-1626

ABSTRACT

Background and Aim: Canine visceral leishmaniasis (CanL) has a broad spectrum of changes, with kidney disease being considered the main cause of mortality. Thus, this study aimed to monitor serum and urinary biomarkers in response to two short-term treatments for CanL.

Materials and Methods: Thirty dogs with CanL were equally divided into two treatment groups and treated with either miltefosine (Group M) or miltefosine plus allopurinol (Group MA); the groups were evaluated before treatment and after 28 days of treatment. Physical exams were performed and hematimetric, biochemical, and urinary parameters, including urinary biomarkers cystatin C (CisC), lipocalin-2 (NGAL), and microalbuminuria, were measured.

Results: Both treatments significantly reduced clinical scores (p<0.05), but only the MA group saw a reduction in the clinical-pathological score. The serum albumin and calcium levels increased significantly in the MA and M groups (p<0.05). Proteinuria and urinary density did not decrease significantly after the treatments. With regard to the biomarkers, CisC and microalbuminuria did not have any significant changes; however, NGAL was significantly reduced in the MA group (p<0.05).

Conclusion: Both pharmacotherapeutic protocols promoted clinical and clinical-pathological improvements. In addition, miltefosine plus allopurinol proved to be a safe treatment due to the lack of changes detected in the monitored renal biomarkers. The treatment with miltefosine plus allopurinol proved to be the most effective, with more pronounced beneficial effects for canines with visceral leishmaniasis.

Keywords: clinical score, cystatin C, Leishmania infantum, miltefosine, NGAL.

Papaya (Carica papaya L.) seed as a potent functional feedstuff for poultry – A review

Review (Published online: 17-08-2020)

18. Papaya (Carica papaya L.) seed as a potent functional feedstuff for poultry – A review

Sugiharto Sugiharto

Veterinary World, 13(8): 1613-1619

ABSTRACT

The steady increase in the price of protein feed ingredients and the retraction of antibiotics from diets has encouraged nutritionists to search the alternatives for protein source and functional feedstuffs that can substitute the role of antibiotic growth promoters in poultry production. With crude protein of 24-30%, in vitro protein digestibility of 80% and proportion of essential amino acids of 47%, seed from ripe papaya may be exploited as the alternative protein feed ingredient for poultry. Moreover, the growth promoting effect, antimicrobial and antiparasitic activities, and immunomodulatory and antioxidative activities may confirm the potential of papaya seed as a functional feedstuff that could replace the role of antibiotic growth promoters for poultry. The in-depth study is needed to further elucidate the functionalities of papaya seed on poultry. This review provides the updates on the nutritional contents of papaya seed, the potential of papaya seed as an alternative to conventional protein-rich ingredient, the growth-promoting effect of feeding papaya seed, the antimicrobial and antiparasitic activities of papaya seed, antioxidative activities of papaya seed, and the immunomodulatory activity of papaya seed on poultry.

Keywords: antimicrobial, antioxidant, antiparasitic, growth promoter, papaya by-product, protein-rich feedstuff.