First report of canine parvovirus molecular detection in Bangladesh

Research (Published online: 29-04-2021)

32. First report of canine parvovirus molecular detection in Bangladesh

F. M. Yasir Hasib, Sharmin Akter and Sharmin Chowdhury

Veterinary World, 14(4): 1038-1043

ABSTRACT

Background and Aim: Canine parvovirus (CPV) is the most important cause of mortality in dogs in many parts of the world. Clinical cases exhibit characteristic signs, including foul-smelling bloody diarrhea, vomiting, fever, and dehydration. This study assessed field and vaccine variants of parvovirus in the Chattogram metropolitan area, Bangladesh. The investigation also aimed to identify risk factors for this disease. This research is the first to identify the presence of CPV in Bangladesh through molecular examination.

Materials and Methods: From October to December 2019, a total of 100 dogs were included in the study. Rectal swabs were taken from all dogs. Twenty dogs showed clinical signs of parvovirus. All clinically affected animals along with 20 randomly selected healthy dogs were tested using amplification refractory mutation system (ARMS)-polymerase chain reaction (PCR) to identify variants from the samples. Logistic regression model analysis was performed to determine the possible risk factors for CPV.

Results: ARMS-PCR showed the presence of all three variants, CPV2a, CPV2b, and CPV2c, in clinically ill dogs, and vaccines available in the study area showed either CPV2a or CPV2b strain. The CPV2c variants showed a higher incidence than the other variants. All apparently healthy animals tested were molecularly negative. Multivariable logistic regression model (generalized linear mixed model) indicated that exotic breeds were 3.83 times more likely to be infected by CPV than local breeds. Furthermore, dogs reared in semi-intensive and extensive management systems were 3.64 and 3.79 times more likely to be infected, respectively, than those reared in an intensive management system.

Conclusion: These findings provide practitioners and pet owners information on the occurrence of different variants and help design effective prevention strategies for CPV infection.

Keywords: Bangladesh, canine parvovirus, CPV2a, CPV2b, CPV2c.

Comparative analysis of canine mesenchymal stem cells and bone marrow-derived mononuclear cells

Research (Published online: 29-04-2021)

31. Comparative analysis of canine mesenchymal stem cells and bone marrow-derived mononuclear cells

Noritaka Maeta, Katsutoshi Tamura, Fuuna Ezuka and Hiroshi Takemitsu

Veterinary World, 14(4): 1028-1037

ABSTRACT

Background and Aim: Mesenchymal stem cells (MSCs), which have multi-lineage differentiation potentials, are a promising source for regenerative medicine. However, the focus of study of MSCs is shifting from the characterization of the differentiation potential to their secretion potential for cell transplantation. Tissue regeneration and the attenuation of immune responses are thought to be affected by the secretion of multiple growth factors and cytokines by MSCs. However, the secretion potential of MSCs profiling remains incompletely characterized. In this study, we focused on the secretion ability related and protein mRNA expression of dog adipose tissue-derived MSCs (AT-MSC), bone marrow (BM)-derived MSCs, and BM-derived mononuclear cells (BM-MNC).

Materials and Methods: Real-time polymerase chain reaction analyses revealed mRNA expression of nine growth factors and seven interleukins in these types of cells and three growth factors protein expression were determined using Enzyme-linked immunosorbent assay.

Results: For the BM-MNC growth factors, the mRNA expression of transforming growth factor-β (TGF-β) was the highest. For the BM-derived MSC (BM-MSC) and AT-MSC growth factors, the mRNA expression of vascular endothelial growth factor (VEGF) was highest. BM-MSCs and AT-MSCs showed similar expression profiles. In contrast, BM-MNCs showed unique expression profiles for hepatocyte growth factor and epidermal growth factor. The three types of cells showed a similar expression of TGF-β.

Conclusion: We conclude that expression of cytokine proteins and mRNAs suggests involvement in tissue repair and protection.

Keywords: bone marrow-derived mononuclear cell, canine, growth factor, interleukin, mesenchymal stem cell, secretion.

Antibiotic resistance of Escherichia coli and Salmonella spp. strains isolated from healthy poultry farms in the districts of Abidjan and Agnibilékrou (Côte d'Ivoire)

Research (Published online: 28-04-2021)

30. Antibiotic resistance of Escherichia coli and Salmonella spp. strains isolated from healthy poultry farms in the districts of Abidjan and Agnibilékrou (Côte d'Ivoire)

Moumouni A. Assoumy, André P. Bedekelabou, Assiongbon Teko-Agbo, Walter Ossebi, Komlan Akoda, Félix Nimbona, Stanislas H. Zeba, Anicet A. Zobo, Raoul C. T. Tiecoura, Vessaly Kallo, Komissiri Dagnogo and Rianatou Bada-Alambédji

Veterinary World, 14(4): 1020-1027

ABSTRACT

Background and Aim: Antimicrobial resistance (AMR) is a serious challenge to animal and human health worldwide. Therefore, this study aims to determine levels and patterns of AMR of Escherichia coli and Salmonella spp. strains isolated from poultry farms in Côte d'Ivoire.

Materials and Methods: A cross-sectional study was conducted in two districts of Côte d'Ivoire with high poultry production: Abidjan and Agnibilékrou. A total of 231 fecal samples were collected in 124 poultry farms in both districts. Enterobacteria were isolated and tested for susceptibility to 14 antimicrobial agents using the disk-diffusion method.

Results: A total of 212 E. coli and 36 Salmonella strains were isolated. In Abidjan, 139 collected samples generated 101 E. coli and 23 Salmonella strains, whereas in Agnibilékrou, 92 collected samples generated 111 E. coli and 13 Salmonella strains. Variable resistance levels were recorded for the antibiotics tested. The resistance prevalence of E. coli and Salmonella, respectively, was high: Doxycycline (98%/94%), sulfonamide (84%/86%), trimethoprim-sulfamethoxazole (80%/41%), and streptomycin (71%/52%). Average resistance rates were recorded for flumequine (38%/66%), ampicillin (49%/33%), amoxicillin (25%/44%), colistin (26%/2%), chloramphenicol (21%/2%), and gentamicin (4%/47%). The antibiotics least affected by resistance were cefuroxime (4%/5%), ceftriaxone (2%/0.00%), and nitrofurantoin (1%/0.00%).

Conclusion: In this study, it was observed that resistance to important antibiotics is emerging in poultry production in Côte d'Ivoire. Policies promoting the rational use of antibiotics should be implemented to manage antibiotic resistance in animal production.

Keywords: antibiotic resistance, Côte d'Ivoire, Escherichia coli, poultry, Salmonella spp.

Assessing factors associated with owner's individual decision to vaccinate their dogs against rabies: A house-to-house survey in Ouagadougou, Burkina Faso

Research (Published online: 27-04-2021)

29. Assessing factors associated with owner's individual decision to vaccinate their dogs against rabies: A house-to-house survey in Ouagadougou, Burkina Faso

Madi Savadogo, Abdoul-Fataf Soré, Laibané Dieudonné Dahourou, Walter Ossebi, Alima Hadjia Banyala Combari, Rianatou Bada Alambedji and Zékiba Tarnagda

Veterinary World, 14(4): 1014-1019

ABSTRACT

Background and Aim: In rabies endemic area, dog vaccination is an effective way of controlling the disease in animals and humans if a minimum of 70% vaccination coverage is reached. This study aimed to identify dog demographics and household characteristics associated with dogs' vaccination against rabies in Ouagadougou, Burkina Faso.

Materials and Methods: A questionnaire was used to collect data from respondents with regard to their dogs' demographics and their household characteristics. Chi-square test and Fisher's exact test were performed to assess the association between explicative variables and the dogs' vaccination status.

Results: Overall, as per the findings of this study, it was determined that out of 424 dogs, 57.8% were reportedly vaccinated. The vaccination status was significantly associated with most of the household variables (e.g., gender of the respondent, age, level of education, main means of transportation, participation in a vaccination campaign, knowledge on rabies, and knowledge on dog vaccination) and the dogs' variables (breed of dog, dog origin, purpose for keeping, confinement status, and perceived behavior) (p<0.05). Moreover, only religion, type of housing, knowledge of rabies transmission modes, and dog sex were not significantly associated with vaccination status (p>0.05).

Conclusion: Our study generated informative data showing that animal health workers could develop effective rabies vaccination strategy planning by examining owned dog demographics and their husbandry practices in households.

Keywords: Burkina Faso, dog vaccination, household survey, owned dogs, rabies control.

First evidence of Klebsiella pneumoniae infection in Aceh cattle: Pathomorphology and antigenic distribution in the lungs

Research (Published online: 27-04-2021)

28. First evidence of Klebsiella pneumoniae infection in Aceh cattle: Pathomorphology and antigenic distribution in the lungs

Darniati Darniati, Surachmi Setiyaningsih, Dewi Ratih Agungpriyono and Ekowati Handharyani

Veterinary World, 14(4): 1007-1013

ABSTRACT

Background and Aim: Klebsiella pneumoniae is an emerging zoonotic and foodborne pathogen worldwide. Hypervirulent K. pneumoniae (hvKp) was reported as the causative agent of bovine mastitis. This is the first study in Indonesia that has been conducted to determine the capsular serotype of K. pneumoniae, pulmonary gross pathology and histopathology, and distribution of hvKp in the lungs of Aceh cattle.

Materials and Methods: The presence of K. pneumoniae in Aceh cattle was investigated in two slaughterhouses in Banda Aceh and Aceh Besar, Indonesia. Lung tissues with gross pathological lesions were collected from 15 cattle presenting with depression, dehydration, or cachexia. The confirmation and capsular serotyping of K. pneumoniae isolates were performed using polymerase chain reaction. The tissues were stained with hematoxylin-eosin and immunohistochemistry to observe the histopathological lesions and the distribution of the hvKp antigens.

Results: The pneumonic lesions identified in the lungs of Aceh cattle included hyperemia, hemorrhage, consolidation, and atelectasis. K. pneumoniae was isolated in all 15 lung tissues with pathological pneumonic lesions. Two patterns of infection were observed histopathologically. Acute infection was characterized by hyperemia, inflammatory cell infiltration, hemorrhage, bronchiolar epithelium hyperplasia, bronchial and bronchiolar obstruction with purulent exudates, edema, and atelectasis. On the other hand, chronic infection was defined by macrophage infiltration, emphysema, bronchial dilatation, pleural fibrosis, and alveolar wall thickening by interstitial fibrosis. Immunohistochemical staining using monospecific antisera induced by the hvKp isolate confirmed the presence of K. pneumoniae-specific antigens in the acute infection, predominantly in the bronchiolar, vascular, and alveolar areas. In contrast, generally diffuse infiltrates were found in the pleura and interstitial alveolar areas in chronic infection.

Conclusion: hvKp can be detected in the lungs of Aceh cattle, representing acute and chronic infections. The distribution of Klebsiella antigens in the lung tissue was consistent with the histopathological findings.

Keywords: Aceh cattle, capsular serotyping, histopathology, immunohistochemistry, Klebsiella pneumoniae, lung.

Determination of natural antibodies, beta-hydroxybutyric acid, and non-esterified fatty acid levels in the serum of peripartum Tuj and Hemşin sheep

Research (Published online: 26-04-2021)

27. Determination of natural antibodies, beta-hydroxybutyric acid, and non-esterified fatty acid levels in the serum of peripartum Tuj and Hemşin sheep

Cihan Kaçar, Semra Kaya, Mushap Kuru, Ekin Emre Erkiliç, Metin Öğün, Hasan Oral and Murat Can Demir

Veterinary World, 14(4): 1002-1006

ABSTRACT

Background and Aim: Many metabolic and immunological changes occur during the transition period. Innate immunity plays an important role against to infections and natural antibodies (NAb) are important in immunity. This study aims to determine a connection between serum NAb titers, beta-hydroxybutyric acid (BHBA), and non-esterified fatty acid (NEFA) concentrations in Tuj and Hemsin sheep during the peripartum period.

Materials and Methods: Serum NAb, BHBA, and NEFA levels were determined from the blood samples collected from Tuj and Hemsin sheep on days 30 and 15 before birth, on the day of birth (day 0), and on days 15 and 30 after birth.

Results: NAb titers were found to be higher in Tuj than in Hemsin sheep (p<0.001). No statistically significant difference was found in serum BHBA concentrations of both breeds on all sampling days (p>0.05). The serum NEFA level was lower in Tuj sheep in the last 15 days of pregnancy compared to Hemsin sheep (p<0.05), while no difference was found in samples collected at the other time points.

Conclusion: This study indicated that serum NAb titers significantly changed in Tuj and Hemsin sheep during the transition period. Serum BHBA and NEFA concentrations increased during the last stages of pregnancy and decreased after birth. Based on these findings, it is suggested that the immunological status could vary by the breed of sheep or various factors that affect the sheep's metabolic state.

Keywords: beta-hydroxybutyric acid, ewe, natural antibody, non-esterified fatty acid, peripartum period.

Detection of Pestivirus in small ruminants in Central Java, Indonesia

Research (Published online: 24-04-2021)

26. Detection of Pestivirus in small ruminants in Central Java, Indonesia

W. Hidayat, H. Wuryastuty and R. Wasito

Veterinary World, 14(4): 996-1001 

ABSTRACT

Background and Aim: Globally, pestiviruses are among the most economically important viral pathogens of livestock. The genus Pestivirus comprises four species, including bovine viral diarrhea virus type 1 and 2 (BVDV-1 and BVDV-2), which infect cattle, border disease virus and classical swine fever virus which infect small ruminants and pigs, respectively. Accumulating evidence suggests that pestiviruses are no longer species-specific, creating new challenges for disease control. In Indonesia, investigations related to pestiviruses remain focused on cattle as the primary host and no research has been conducted on small ruminants (sheep and goats). Therefore, the present study aimed to study the possible occurrence of pestivirus (BVDV or BVD) infections in small ruminants in Indonesia, particularly in Central Java.

Materials and Methods: We used 46 blood samples consisting of 26 sheep's blood and 20 goat's blood. Samples were selected from 247 small ruminant blood collected between July and October 2020 in Central Java, Indonesia, which met the following criteria: Female, local species, approximately 1-2 years old, never been pregnant, raised in the backyard, and had no close contact with cattle in either shelter or grazing area. We tested plasma samples from sheep and goats using competitive antibody enzyme-linked immunosorbent assay to detect specific antibodies against pestivirus followed by reverse transcription-polymerase chain reaction (RT-PCR) analysis for all positive samples to differentiate the species of pestivirus.

Results: Two of the 20 samples collected from goats were positive for pestivirus at the serological and molecular levels, whereas 2 of 26 samples collected from sheep were doubtful but tested negative by RT-PCR. The genotyping test results obtained using nested PCR revealed that the positive samples collected from goats had a BVDV-1 genotype.

Conclusion: The results of the present study demonstrated that BVDV-1 can infect species other than bovines, in Central Java, Indonesia. Further studies involving a larger number of samples are required to: (1) Determine the actual seroprevalence of pestiviruses in small ruminants and (2) Determine the potency of small ruminants as reservoirs for pestiviruses, both of which are important for the identification of the appropriate control program for pestiviruses in Indonesia.

Keywords: antibody enzyme-linked immunosorbent assay, bovine viral diarrhea virus, pestivirus, reverse transcriptase-polymerase chain reaction, small ruminants.