Susceptibility of virulent and resistant Escherichia coli strains to non-polar and polar compounds identified in Microplumeria anomala

Research (Published online: 21-07-2020)

18. Susceptibility of virulent and resistant Escherichia coli strains to non-polar and polar compounds identified in Microplumeria anomala

Livia Roberta Piedade Camargo, Vania Maria de Carvalho, Ingrit Elida Collantes Díaz, Mateus Luís Barradas Paciencia, Sergio Alexandre Frana, Riad Naim Younes, Antonio Drauzio Varella, Luiz Fernando Lima Reis and Ivana Barbosa Suffredini

Veterinary World, 13(7): 1376-1387

ABSTRACT

Background and Aim: Escherichia coli is one of the main pathogens responsible for veterinary and human infections, and it is associated with significant economic losses in the livestock, as it causes severe diseases to humans, particularly in children. For that reason, there is a need for introducing new drugs to treat E. coli diseases. The Brazilian species richness is a source of potential new antibacterial natural products. The study aimed at the biological and chemical investigation of the organic extract obtained from the stem of Microplumeria anomala (Apocynaceae), EB127, as it was identified as a potential source of new antibacterial compounds to be used in Veterinary.

Materials and Methods: The antibacterial activity was evaluated by disk diffusion and microdilution assays; chromatography, nuclear magnetic resonance spectrometry, and mass spectrometry were used in the isolation and identification of compounds.

Results: EB127 showed activity against E. coli ATCC25922, and against three E. coli strains that were isolated from frigarte's cloaca, named 31/1A, 35A, and 51A. Lupeol, 3-acetyl-11-oxo-β-amyrin, 3-acetyl-11-oxo-α-amyrin, sitosterol, stigmasterol, 3β,7α-dihydroxy-cholest-5-ene, 3β-hydroxy-cholest-5-en-7-one, and 3β-hydroxy-cholest-5,22-dien-7-one were identified in fraction Hex/CHCl3, while loganin, loganic acid, methylanomaline, and anomaline were all identified in EB127 and protocatechuic acid hexoside, ferulic acid, secoxyloganin, feruloylquinic acid, vanillic acid hexoside, protocatechuic acid-4-O-β-hexoside, and rosmarinic acid were tentatively identified in fraction 10%ACN/H2O. E. coli 51A (virulent/non-resistant) showed sensitivity to the antibacterial action of fraction Hex/CHCl3 which contains alkaloids, triterpenes, and steroids, while E. coli 35A (resistant/non-virulent) were more susceptible to 10%ACN/H2O, which contains iridoids as loganin and loganic acid, and glycosylated and non-glycosylated caffeic acids.

Conclusion: Fraction 10%ACN/H2O is of interest in pursuing new drugs to treat resistant E. coli, in veterinary. All compounds were isolated from the plant for the first time and have shown potential as new antibacterial natural products from Amazon plants to be used in veterinary and human diseases.

Keywords: antibacterial agents, companion animals, livestock, plant extracts, poultry, tropical rainforest.

Isolation of peripheral blood mononuclear cells and the expression of toll-like receptors in Betong chickens

Research (Published online: 18-07-2020)

17. Isolation of peripheral blood mononuclear cells and the expression of toll-like receptors in Betong chickens

Anutian Suklek, Autchara Kayan, Jatuporn Rattanasrisomporn and Chaiwat Boonkaewwan

Veterinary World, 13(7): 1372-1375

ABSTRACT

Background and Aim: Toll-like receptors (TLRs) comprise microbial sensing receptors present on cell surfaces that are capable of detecting pathogens. The present study aims to examine the expression of TLRs within the peripheral blood mononuclear cell (PBMC) of the Betong chickens.

Materials and Methods: Blood samples were harvested from 12 Betong (KU line) chickens. Hematological values were calculated. PBMC was isolated from the blood utilizing a Histopaque solution and stored in a RPMI1640 culture medium. Cell viability was investigated using a Trypan Blue dye exclusion test. DNA was extracted from PBMC and the expression of the DNA's TLRs was examined using a polymerase chain reaction.

Results: Hematological values were determined from the blood samples collected in this study obtained from healthy Betong chickens. PBMC that was isolated from the Betong chickens possessed cell viability higher than 95% (95.37±1.06). From the examination of TLRs gene expression, results revealed instances of TLR1.1, TLR1.2, TLR2.1, TLR2.2, TLR3, TLR4, TLR5, TLR 7, TLR15, and TLR21 that were present in the PBMC of Betong chickens.

Conclusion: PBMC isolated from the blood of healthy Betong chickens possessed excellent cell quality. All chicken TLRs were discovered within the PBMC of Betong chickens. Hence, PBMC stands out as one of the premier sources for in vitro studies of chicken immune response.

Keywords: Betong chicken, peripheral blood mononuclear cell, toll-like receptor.

Microbiological quality of beef, mutton, and water from different abattoirs in the Eastern Cape Province, South Africa

Research (Published online: 18-07-2020)

16. Microbiological quality of beef, mutton, and water from different abattoirs in the Eastern Cape Province, South Africa

Philisani Ncoko, Ishmael Festus Jaja and James Wabwire Oguttu

Veterinary World, 13(7): 1363-1371

ABSTRACT

Background and Aim: Abattoir processes from skinning, evisceration, to chilling usually lead to meat contamination by foodborne pathogens. Hence, continual microbial surveillance of slaughter carcasses by veterinary public health officials is key to preventing contamination and outbreak of meat-related foodborne diseases. This study was conducted to determine the Enterobacteriaceae count and aerobic plate count (APC) and to detect Escherichia coli and Salmonella spp. in meat and water from selected slaughter facilities.

Materials and Methods: Retrospective data (n=100) collected in 2017 by the Provincial Veterinary Department of the Eastern Cape Province from abattoirs and prospective survey data of meat (n=50) collected in 2018 from abattoirs in the Eastern Cape Province were utilized in this study. APC and Enterobacteriaceae were enumerated from the samples. In addition, Salmonella and E. coli were isolated from samples using selective media.

Results: The APC in both retrospective and prospective studies for all samples ranged between 2 and 4.50 log CFU/cm2; similar counts of 2-4.00 log CFU/cm2 were recorded for Enterobacteriaceae. No significant difference (p>0.05) for APC and Enterobacteriaceae count across all meat types was noted. Salmonella and E. coli were detected in 50% of beef. E. coli was not detected from mutton, but Salmonella was found in 66.7%. Moreover, 91.7% of the water samples had E. coli, but none had Salmonella.

Conclusion: The levels of Enterobacteriaceae and APC observed in meat satisfy regulatory conditions outlined by the Department of Agriculture, Forestry and Fisheries, South Africa and show that meat produced from these abattoirs is of acceptable microbial quality. However, the quality of water used in the abattoirs does not meet the requirements set by the government, and contributes to contamination of meat produced in the abattoirs under study. Therefore, we recommend that sources of water be continuously investigated to eliminate or reduce the risk of contamination of meat processed in the abattoirs.

Keywords: contamination, foodborne pathogens, hygiene, meat spoilage, water quality.

Sequencing and phylogenetic analysis of infectious bronchitis virus variant strain from an outbreak in egg-layer flocks in Baghdad, Iraq

Research (Published online: 16-07-2020)

15. Sequencing and phylogenetic analysis of infectious bronchitis virus variant strain from an outbreak in egg-layer flocks in Baghdad, Iraq

Abdullah O. Alhatami, Furkan Alaraji, Husam Muhsen Abdulwahab and Yahia Ismail Khudhair

Veterinary World, 13(7): 1358-1362

ABSTRACT

Background and Aim: Infectious bronchitis (IB) has an influential economic impact on the poultry industry, causing huge losses each year due to the condemnation of infected chickens. Despite the use of many kinds of vaccines in Iraq, it is common to find IB problems in vaccinated chickens. Information about the strains that affect Iraqi chickens is very limited. Therefore, we aimed to detect the currently circulating strains of IB virus that cause frequent outbreaks in egg layers despite the use of vaccination against the virus.

Materials and Methods: Isolate detection, sequencing, and phylogenetic analysis were performed using a rapid IB virus antigen kit (32 tracheal swabs), flinders technology associates (FTA) card (32 tracheal swabs), and partial gene sequencing (16 positive FTA samples).

Results: The isolated strain was different from other strains, especially the strain isolated in the North of Iraq (Sulemania Strain) and shares 98% homology with an Israeli strain (Israel variant 2, IS 1494).

Conclusion: Although more studies are needed to detect IB virus strains circulating in Iraq, this work lays the foundation for making a good strategy to control the disease and selecting vaccines that should be used in farms.

Keywords: infectious bronchitis, phylogenetic tree, poultry, real-time polymerase chain reaction, spike 1 gene.

Infection with Babesia canis in dogs in the Algiers region: Parasitological and serological study

Research (Published online: 15-07-2020)

14. Infection with Babesia canis in dogs in the Algiers region: Parasitological and serological study

Amel Kiouani, N. Azzag, S. Tennah and F. Ghalmi

Veterinary World, 13(7): 1351-1357

ABSTRACT

Background and Aim: Canine babesiosis is a vector-borne disease transmitted by ticks of the Ixodidae family. The effects of infection in dogs can range from the subclinical to the severe lethal form. This study aimed to make an original contribution to the knowledge of circulating species of Babesia spp. in dogs in the region of Algiers as well as mechanisms and risk factors for their transmission.

Materials and Methods: An epidemiological study was carried out on 189 blood samples taken from dogs from April 2015 to January 2016. The samples taken underwent parasitological by Giemsa-stained blood smear and serological analyzes by indirect fluorescent antibody test (IFAT). The ticks were looked on all the dogs taken.

Results: Giemsa-stained blood smears revealed the presence of two groups of parasites of the genus Babesia: Large Babesia (3/25, 12%) and small Babesia (22/25, 88%). The IFAT at a dilution of 1/32 showed an overall seroprevalence with Babesia canis of 17.98% (95% confidence interval 11.53-22.46). The distribution of the antibody titers for the positive samples showed that of the 34 positive sera with a titer ≥1/32, 28 sera remained positive at a dilution of 1/64 (14.81%), 22 at a dilution of 1/128 (11.64%) and 15 sera remained positive at a dilution of 1/256 (7.93%). Although seroprevalence varied according to canine population (20% and 19.49% in pet dogs and canine pound dogs, respectively, and 6.66-0% in farm dogs and hunting dogs, respectively), statistical analysis showed no significant differences between populations. The antibody titers obtained after several dilutions showed that 22 canine pound dog sera remained positive at a dilution of 1/128 compared to pet dogs and farm dogs which ceased to be positive at the dilution of 1/64. The comparison between the two diagnostic methods showed a strong agreement between the parasitological examination by blood smear and the serological method by IFAT. However, IFAT was much more sensitive. The analysis of risk factors, which may influence B. canis seroprevalence, has shown the influence of age, tick presence, and season. Finally, of the 242 ticks collected from a total of 59 dogs, only one tick species was identified, Rhipicephalus sanguineus.

Conclusion: This study indicates a frequent circulation of species of Babesia in the dog in the Algiers region and R. sanguineus was the only tick identified.

Keywords: Algiers, Babesia canis, Babesia spp., blood smears, dogs, prevalence, risk factors, serology, ticks.

The epidemiological background of small ruminant lentivirus infection in goats from Romania

Research (Published online: 15-07-2020)

13. The epidemiological background of small ruminant lentivirus infection in goats from Romania

Adrian-Valentin Potarniche, Constantin Gheorghe Cerbu, Michal Czopowicz, Olga Szalus-Jordanow, Jaroslaw Kaba and Marina Spinu

Veterinary World, 13(7): 1344-1350

ABSTRACT

Background and Aim: Caprine arthritis-encephalitis (CAE) is an economically significant viral disease of goats caused by a small ruminant lentivirus (SRLV) belonging to Retroviridae family. This study aimed to summarize current information on the epidemiological status of SRLVs infection in the population of goats from Romania and to point out the CAE incidence throughout the 2008-2018 periods.

Materials and Methods: An exhaustive review of the papers published in the international literature concerning the epidemiological status of CAE in Romania was carried out using electronic databases, and available statistical data from the World Organization for Animal Health (OIE) regarding the incidence of the disease between 2008 and 2018 were analyzed.

Results: The true individual-level seroprevalence of CAE was estimated in 13 of 42 counties (31%) and ranged from 0.4% to roughly 40%. One hundred eighty-two outbreaks from 14 counties (33%) were reported, with a peak in 2010.

Conclusion: The findings sourcing in the literature are very scarce and show disagreement with the situation reported by the national veterinary authorities. Lack of SRLVs screening policies represents the main obstacle in limiting the spread of the disease. Romania's National Sanitary Veterinary and Food Safety Authority should implement a program for diagnosis and surveillance of the disease to build a straightforward epidemiological picture that represents a prerequisite of any control and eradication program.

Keywords: epidemiology, goat, outbreaks, small ruminant lentivirus.

Effects of polymorphism of myostatin and fatty acid-binding protein 4 genes on the chemical composition of meat in cull female Aceh cattle

Research (Published online: 14-07-2020)

12. Effects of polymorphism of myostatin and fatty acid-binding protein 4 genes on the chemical composition of meat in cull female Aceh cattle

Al Azhar, Muslim Akmal, Muhammad Hambal, Mustafa Sabri and Teuku Shaddiq Rosa

Veterinary World, 13(7): 1334-1343

ABSTRACT

Aim: This study aimed to investigate the association of single nucleotide polymorphism of the myostatin (MSTN) and fatty acid-binding protein 4 (FABP4) genes on the total water, ash, fat, protein, and cholesterol contents of sirloin (gluteus medius muscle) and silverside (biceps femoris muscle) meats of cull female Aceh cattle.

Materials and Methods: This analysis covered a total of 27 cull female Aceh cattle slaughtered at the Animal Slaughterhouse of Banda Aceh that was purposively selected based on hair color referred to the criteria described in the Decree of Ministry of Agriculture of the Republic of Indonesia. Genomic DNA was extracted from 25 mg of fresh meat using the spin column method before subjected to a polymerase chain reaction amplification using primer sets specific for 1346-bp and 275- bp fragments of MSTN and FABP4, respectively. A 4-h digestion reaction was done separately for the MSTN/HaeIII and FABP4/NlaIII loci genotyping. The total protein, ash, and fat of the meat were measured using the Indonesian National Standard (SNI) methods whereas its cholesterol content was determined using the AOAC method. The association between each polymorphism and the variation in meat chemical parameters was analyzed using the Pearson correlation test.

Results: The results showed that the MSTN/HaeIII locus was polymorphic in Aceh cattle, but the FABP4/NlaIII locus was monomorphic. Meat chemical parameters were not influenced by different commercial cuts and MSTN genotypes, showing that there was no association between different commercial cuts, cattle hair colors, and MSTN/HaeIII and FABP4/NlaIII markers with the meat chemical parameters in Aceh cattle.

Conclusion: These results suggest that focusing on the novel effects of MSTN and FABP4 gene polymorphisms on meat production traits might not be useful for marker-assisted selection in Aceh cattle.

Keywords: Aceh cattle meat, ash, cholesterol, fat, polymerase chain reaction-restriction fragment length polymorphism, protein.