Thursday 6 May 2021

Study on bacterial pathogens through multiplex polymerase chain reaction system and their antimicrobial resistance pattern in goats presumed with fever and/or diarrhea

Research (Published online: 06-05-2021)
4. Study on bacterial pathogens through multiplex polymerase chain reaction system and their antimicrobial resistance pattern in goats presumed with fever and/or diarrhea
Pranab Paul, Md. Rayhan Faruque, Md. Kaisar Rahman, Priyanka Das and Mohammed Yousuf Elahi Chowdhury
Veterinary World, 14(5): 1080-1092

ABSTRACT

Background and Aim: Goat is one of the major livestock species, plays an important role in the economy of Bangladesh. However, the outbreak of different infectious diseases in goats causes high mortality and economic losses due to lack of proper diagnosis and treatment. Conventional culture-based methods for detecting specific pathogens as confirmatory diagnosis are laborious as well as time-consuming in comparison to multiplex polymerase chain reaction (mPCR), by which multiple pathogens can be detected at a time. The present study was aimed to perform faster molecular identification of bacterial pathogens from goats presumed with fever and/or diarrhea and their antimicrobial resistance (AMR) pattern.

Materials and Methods: A total of 200 blood samples were collected from goats at S. A. Quaderi Teaching Veterinary Hospital (SAQTVH) in Chattogram Veterinary and Animal Sciences University for the period of July 2017-April 2018. DNA was extracted and subsequently, mPCR assay was performed for the screening of several bacterial pathogens (Salmonella spp., Listeria monocytogenesBacillus cereusYersinia enterocoliticaCampylobacter jejuniCampylobacter coliClostridium perfringensVibrio cholerae, and Staphylococcus aureus). An antimicrobial susceptibility test against ten antimicrobials for positive samples of each organism was conducted using the Kirby–Bauer Disk-Diffusion Method on selective media.

Results: S. aureusC. perfringensL. monocytogenes, and Salmonella spp. were detected from collected samples and their overall prevalence was 11.5%, 3.5%, 1%, and 20.5%, respectively. The most common clinical signs were mild fever, nasal discharge, dyspnea, and coughing (39.1%) for S. aureus, diarrhea, convulsion, abdominal pain, and incoordination (57.1%) for C. perfringens, fever, protrusion of tongue, and incoordination (100%) for L. monocytogenes, and fever, anorexia, dehydration with mucous feces (36.6%) for Salmonella spp. infection in goats. Antimicrobial diagram of S. aureus showed resistance against Cefotaxime (74%), Cefixime (65%), and Tetracycline (65%); highly sensitive against Amoxicillin (48%), Ciprofloxacin (44%), and Gentamicin (44%). On the other hand, C. perfringens showed highly resistant against Ampicillin (71%), Gentamicin (71%), sensitive against Penicillin (57%), and Cefotaxime (57%). L. monocytogenes were found to be sensitive to Penicillin (100%) and Cefixime (100%) and Salmonella spp. showed resistance to Ampicillin (78%) and Amoxicillin (59%) but sensitive to Ciprofloxacin (54%).

Conclusion: This study identified pathogens with their specific clinical signs in goats presumed fever and/or diarrhea through mPCR with their AMR pattern in SAQTVH, Chattogram. Potential risk factors, measuring the strength of association of disease caused by these particular pathogens, were also determined. mPCR may use as an effective tool for rapid detection of pathogens in animal.

Keywords: antimicrobial resistance, goat, infectious disease, multiplex polymerase chain reaction, prevalence.



Wednesday 5 May 2021

Antimicrobial susceptibility profiles of Staphylococcus spp. contaminating raw goat milk

Research (Published online: 05-05-2021)
3. Antimicrobial susceptibility profiles of Staphylococcus spp. contaminating raw goat milk
Abimael E. Silva Júnior, Priscylla C. Vasconcelos, Mauro M. S. Saraiva, Lauro Santos Filho, Núbia M. V. Silva, Patricia E. N. Givisiez and Celso J. B. Oliveira
Veterinary World, 14(5): 1074-1079

ABSTRACT

Background and Aim: Antimicrobial resistance poses a major threat to global public health. Foodstuff of animal origin can serve as potential vehicles for the dissemination of antimicrobial-resistant bacteria and resistance genes to consumers. In view of the lack of knowledge about antimicrobial resistance in bacteria associated with goat milk, the aim of this study was to report species-level identification and antimicrobial susceptibility profiles of a large collection of Staphylococcus spp. isolates recovered from raw goat milk in Brazil.

Materials and Methods: A total of 434 Staphylococcus spp. isolates originated from 510 goat milk samples in Northeast Brazil were investigated. The isolates were obtained by conventional microbiological methods. Species identification and antimicrobial susceptibility testing were performed by means of a semi-automated system using a panel for biochemical tests and broth microdilution method for 19 antimicrobial drugs.

Results: Although Staphylococcus aureus (22.6%) accounted for the majority of the isolates, a total of 13 different non-aureus staphylococci spp. were identified. High resistance rates against erythromycin (40.8%), and the beta-lactams ampicillin (45.9%) and penicillin (42.9%) were observed among S. aureus isolates. The most significant findings were related to the resistance against quinupristin-dalfopristin, a drug of last resort used in human medicine to treat infections caused by vancomycin-resistant S. aureus and enterococci.

Conclusion: The high diversity of Staphylococcus spp. showing phenotypic resistance against different antimicrobial drugs encourages further investigations on the real impact of these bacteria as reservoirs of antimicrobial resistance genes to consumers. Furthermore, the potential impact of technological processes, such as pasteurization, fermentation, and maturation, on the maintenance and dissemination of antimicrobial resistance among the microbial populations in milk and dairy products must also be investigated.

Keywords: antimicrobial resistance, dairy goats, food safety, Staphylococcus.



Tuesday 4 May 2021

Comparative evaluation of isoflurane and sevoflurane in avian patients

Research (Published online: 04-05-2021)
2. Comparative evaluation of isoflurane and sevoflurane in avian patients
R. R. Anjana, P. V. Parikh, J. K. Mahla, D. N. Kelawala, K. P. Patel and S. N. Aswath
Veterinary World, 14(5): 1067-1073
Comparative evaluation of isoflurane and sevoflurane in avian patients

ABSTRACT

Background and Aim: Literature comparing the use of isoflurane and sevoflurane inhalation anesthetic agents in birds is scarce. This study aimed to evaluate the comparison of isoflurane and sevoflurane during induction, maintenance, and recovery of anesthesia in avian patients.

Materials and Methods: In this study, 24 injured avian patients (n=24) were selected randomly and divided into four groups during kite flying festival. In the present study, isoflurane and sevoflurane were used as induction and maintenance anesthetic agents, with and without butorphanol tartrate premedication agent in all the birds. Different physiological parameters were evaluated, namely, cloacal temperature (°F), heart rate (beats/min), respiratory rate (breaths/min), and SpO2 (%) were recorded at 0, 10, 20 min, and at recovery time. The quality of anesthesia was assessed on the basis of induction time, quality of induction, production of analgesia, muscle relaxation, body reflexes, recovery time, quality of recovery, sitting, standing, and complete recovery time (CRT).

Results: The mean±standard error value of induction time was 230.00±32.55, 280.00±25.29, 180.00±21.90, and 260.00±36.87 s, respectively, in Groups I, II, III, and IV. The feather plucking, pharyngeal, and toe pinching reflexes were noticed, when the birds were passing through the light plane of anesthesia during induction. Comparison of cloacal temperature at the time of recovery between Group-I versus Group-III revealed a significant difference (p<0.05). Comparison of mean respiratory rates at the time of recovery between Group-II versus Group-IV revealed a significant difference (p<0.05). Excellent quality of recovery was observed in all the groups of anesthetic protocols. Sitting, standing, and CRT were observed shortest in avian patients maintained with sevoflurane as compared to isoflurane.

Conclusion: The quality of induction of anesthesia was rapid in avian patients when induced with sevoflurane as compared to isoflurane. Rapid onset of induction and recovery of anesthesia were found with sevoflurane followed by isoflurane. Induction and maintenance of anesthesia in avian patients with sevoflurane resulted in the lowest time required for sitting, standing, and CRT.

Keywords: anesthesia, avian patients, comparison, isoflurane, sevoflurane.

The use of carotene-containing preparation in cows for the prevention of postpartum complications

Research (Published online: 04-05-2021)
1. The use of carotene-containing preparation in cows for the prevention of postpartum complications
Tatyana Vladimirovna Zubova, Vladimir Alexandrovich Pleshkov, Oksana Vladimirovna Smolovskaya, Alexander Nikolaevich Mironov and Larisa Nikolaevna Korobeynikova

Veterinary World, 14(5): 1059-1066 

The use of carotene-containing preparation in cows for the prevention of postpartum complications

ABSTRACT

Background and Aim: It is known that metabolic disturbances in the animal body negatively affect parturition, uterine involution, and, ultimately, fertility, especially in cows, during the first insemination. Although the method of diet optimization with the use of a software package results in positive outcomes, adjustment is required for certain groups of animals (e.g., cows), in accordance with the period of the year. Every year, in the spring and autumn, blood is taken from animals to detect metabolic disorders, and then either the diet is balanced or, if the cows lack vitamins and minerals, they are administered to cows parenterally or with food. The aim of this study was to assess the efficiency of using carotene-containing preparation in the prevention of postpartum complications in cows.

Materials and Methods: Before the start of the experiment, blood was collected from the animals of the experimental and control groups, with ten animals in each group, and its serum was examined for the presence of carotene. Low carotene content was found in the serum of all animals (from 0.2 to 0.25 mg%) with the norm within the range of 0.40-0.62 mg%. The cows of the experimental group were injected subcutaneously with the carotene-containing preparation 30, 20, and 10 days before the expected calving date at a dose of 10 mL per head. The carotene-containing preparation was a solution of crystalline β-carotene substance in deodorized sunflower oil. Moreover, the share of β-carotene was at least 0.18%. The drug was administered intramuscularly into the rump.

Results: In the postpartum period, the retention of the placenta was observed in two animals of the control group. The uterine involution in the cows of the control group was 16.0 (p<0.05) days longer than that in the cows of the experimental group. The duration of placenta separation in the cows of the control group was on average 3.21 h longer (p<0.01) than that in the cows of the experimental group. The period from calving to the introduction of the embryo was 63.17±1.56 days in the control group and 48.3±0.83 days in the experimental group. The survival rate of embryos in the cows of the experimental group was 60%, and the period from the calving date to the introduction of the embryo averaged 48 days, which were 14.9 (p<0.05) days less than that in the cows of the control group.

Conclusion: When the carotene-containing preparation was administered in a dose of 10 mL subcutaneously to cows 30, 20, and 10 days before the calving date, the blood carotene content increased, and the duration of the last stage and uterine involution decreased. The period from the calving date to the introduction of the embryo was reduced to 48.3±0.83 days, and the survival rate of embryos was 60%.

Keywords: carotene, diet, embryos, feeding, involution, uterine.

Monday 3 May 2021

Understanding eco-immunology of bacterial zoonoses and alternative therapeutics toward "One Health"


Review 
(Published online: 03-05-2021)
13. Understanding eco-immunology of bacterial zoonoses and alternative therapeutics toward "One Health"
Manisha Behera, Soma Mondal Ghorai, Sachinandan De and Hardeep Kaur

International Journal of One Health, 7(1): 104-115 

Understanding eco-immunology of bacterial zoonoses and alternative therapeutics toward "One Health"

ABSTRACT

The current review identifies key bacterial zoonoses, the understanding of comparative immunology, evolutionary trade-offs between emerging bacterial pathogens and their dynamics on both arms of immunity. The several gaps in the literature limit our understanding of spread of prominent bacterial zoonotic diseases and the host-pathogen interactions that may change in response to environmental and social factors. Gaining a more comprehensive understanding of how anthropogenic activities affects the spread of emerging zoonotic diseases, is essential for predicting and mitigating future disease emergence through fine-tuning of surveillance and control measures with respect to different pathogens. This review highlights the urgent need to increase understanding of the comparative immunity of animal reservoirs, design of vaccines according to the homology in host-pathogen interactions, and the alternative strategies to counter the risk of bacterial pathogenic spillover to humans with eventual spread of zoonotic diseases.

Keywords: alternative antimicrobials, bacterial zoonoses, comparative immunology, One Health.


One Health information management: health system reforms to support social well-being in Thailand

Research (Published online: 03-05-2021)
12. One Health information management: health system reforms to support social well-being in Thailand
Sangtien Youthao and Somsak Amornsiriphong
International Journal of One Health, 7(1): 96-103
One Health information management: health system reforms to support social well-being in Thailand
ABSTRACT

Background and Aim: The information about the health problems interaction between animal health, ecosystems, and human health that the "One Health (OH)" concept is becoming more complex. This study focused on OH information in Thailand to develop guidelines for establishing a "One Health Information Management (OHIM)" system by drafting the structure of an OH information strategic plan.

Materials and Methods: A mixed methods approach was used and included questionnaires, interviews, focus groups, and observations in agencies across the country to gather evidence about actions relating to the OH concept, such as the Memorandum of Agreements between the Ministry of Public Health and eight main agencies.

Results: The study concentrated on the key issue that understanding the OH concept is difficult, as the environment and human health sectors include less knowledge of this concept than the animal health sector. Further, there are concerns about the comprehension of OH concepts, and high-level information management and data storage relating to OH, including medicine, cattle, wild animal, environment, and environmental resource management. Data from OH researchers and publicly accessible government data are less integrated and inconsistently managed across agencies.

Conclusion: The study of OHIM strategic development should consist of four important points: (1) Clarity in the OH concept, (2) OH staff development, (3) development of an OHIM data network and innovation, and (4) research and academic development, and global OHIM academic exchange. All of these points will lead to health system reforms to support social well-being.

Keywords: health information management, health, one health information, one health, social well-being, Thailand.

Friday 30 April 2021

Risk factors associated with acute hepatopancreatic necrosis disease at shrimp farm level in Bac Lieu Province, Vietnam

Research (Published online: 30-04-2021)
34. Risk factors associated with acute hepatopancreatic necrosis disease at shrimp farm level in Bac Lieu Province, Vietnam
Hien The Nguyen, Toan Nguyen Van, Tien Tien Ngoc, Visanu Boonyawiwat, Theera Rukkwamsuk and Adisorn Yawongsa
Veterinary World, 14(4): 1050-1058
Risk factors associated with acute hepatopancreatic necrosis disease at shrimp farm level in Bac Lieu Province, Vietnam

ABSTRACT

Background and Aim: Acute hepatopancreatic necrosis disease (AHPND) is a severe disease in shrimp farms and adversely affected the shrimp industry of Vietnam. So far, the study on risk factors associated with AHPND outbreaks is limited. The objective of this study was to determine the potential risk factors of AHPND at the shrimp farm level in Bac Lieu Province, Vietnam.

Materials and Methods: Real-time-Polymerase chain reaction was used to analyze data collected from an active surveillance program of shrimp farms in 2017 in the Vinh Tien and Vinh Lac villages, Vinh Thinh commune, Hoa Binh district in Bac Lieu Province, Vietnam. The matched case-control study selected 20 cases and 20 control farms from 134 shrimp farms. In 2018, face-to-face interviews using structured questionnaires were conducted with the farmers of these selected farms.

Results: Of the 59 studied variables, seven had p≤0.2 based on bivariate analyses. The results of multivariable analysis showed that the presence of fish-eating birds on shrimp farms was a significant association with AHPND (odds ratio=8, p=0.049).

Conclusion: To reduce the effect of AHPND, farmers should apply effective methods to manage wild animals such as using a grid or net to cover the pond, combined with improved biosecurity.

Keywords: conditional logistic regression, early mortality syndrome, fish-eating bird, matched case-control study, odd ratio.