The concentration of androgen receptor and protein kinase A in male chicken following the administration of a combination of the epididymis and testicular extracts

Research (Published online: 15-08-2020)

15. The concentration of androgen receptor and protein kinase A in male chicken following the administration of a combination of the epididymis and testicular extracts

Muslim Akmal, Gholib Gholib, Mustafa Kamal Nasution, Sri Wahyuni, Rinidar Rinidar, Dian Masyitha and M. Aman Yaman

Veterinary World, 13(8): 1594-1598

ABSTRACT

Background and Aim: Testis (T) and epididymis (E) are waste from the abattoir that is rarely used. In fact, both organs contain important chemicals needed for spermatogenesis (e.g., hormones, proteins, and other molecules). Therefore, administration of a combination of testis and epididymis (CTE) extracts may activate androgen receptors (AR) and protein kinase A (PKA) molecules that play a prominent role in spermatogenesis. We, therefore, aimed at investigating the influence of the CTE extracts on the concentration of AR and PKA in male chicken.

Materials and Methods: This study used a completely randomized design with four treatment groups (K0, K1, K2, and K3) and five replications per group. K0 is a control group that received 1 mL normal saline, whereas K1, K2, and K3 are the test groups that received 1, 2, and 3 mL of CET extracts, respectively. Twenty male chickens (strain: broiler Mb 89), 3 weeks of age, weighing 500-700 g were used. We administered the injections in a 13-day period and on the 14th day; we collected and processed blood samples as serum to measure the AR and PKA concentrations using commercial chicken AR and PKA enzyme-linked immunosorbent assay kits, respectively. We performed analyses by analysis of variance using SPSS 20.0.

Results: The AR concentrations in K1, K2, and K3 groups increased by 4.26%, 10.97%, and 28.04%, respectively, compared to the K0 (control group). However, this increase was not significantly different between the groups (p>0.05). Moreover, the PKA concentrations increased by 2.97%, 2.60%, and 4.08% in K1, K2, and K3 groups, respectively, compared to the control group. However, this increase was not significantly different between the groups as well (p>0.05).

Conclusion: The CTE extracts tended to increase the AR and PKA concentrations even though it is not significant. Therefore, it needs further study when using the CTE extracts for spermatogenesis in male chicken.

Keywords: androgen receptor, chicken, epididymis and testicular extracts, protein kinase A, spermatogenesis.

Molecular characterization of antimicrobial resistance and virulence genes of Escherichia coli isolates from bovine mastitis

Research (Published online: 14-08-2020)

14. Molecular characterization of antimicrobial resistance and virulence genes of Escherichia coli isolates from bovine mastitis

Zuhair Bani Ismail and Sameeh M. Abutarbush

Veterinary World, 13(8): 1588-1593

ABSTRACT

Background and Aim: Mastitis is a common and economically important disease in dairy cattle. It remains one of the most common reasons for the extensive use of antimicrobials in dairy farms leading to the emergence of antimicrobial-resistant pathogens. The aim of this study was to determine the patterns of antimicrobial resistance of Escherichia coli isolates from bovine mastitis and to identify prominent antimicrobial resistance and virulence genes among isolated strains.

Materials and Methods: Antimicrobial susceptibility testing against six antibiotic groups, including tetracyclines, aminoglycosides, beta-lactams, macrolides, sulfonamides, and fluoroquinolones was performed using the disk diffusion method. PCR was performed on resistant isolates to detect resistance and virulence genes using commercially available primers.

Results: Out of 216 milk samples cultured, 14 samples yielded E. coli isolates. All isolates (100%) were resistant to ampicillin, amoxicillin, procaine penicillin, streptomycin, oxytetracycline, and sulfamethoxazole-trimethoprim. Only one isolate (7%) was sensitive to gentamicin, and all isolates (100%) were sensitive to enrofloxacin and ciprofloxacin. All isolates carried at least one resistance gene against one or more of the major antibiotic groups. All isolates carried the ereA, tetG, tetE, and tetB genes, followed by tetA (93%), ampC (86%), strA (86%), sul1 (78%), tetD (71%), tetC (57%), aadA (57%), and strB (36%). The lowest percentage of isolates carried bla1 (17%) and bla2 (12%) genes, and none of the isolates carried the qnrA gene. Most of the isolates (93%) carried the Shiga toxin 1 virulence gene, followed by complement resistance protein (79%), intimin (64%), Shiga toxin 2 (36%), cytotoxic necrotizing factor (35%), aerotaxis receptor (21%), and type 1 fimbriae (15%).

Conclusion: Results of this study indicate that the high percentages of E. coli isolate from bovine mastitis are resistant to two or more of the major antibiotic groups, irrespective of the presence or absence of relevant resistance or virulence genes.

Keywords: antimicrobial resistance, dairy cows, environmental mastitis pathogens, Escherichia coli.

Description of normal head structures of the one-humped camel (Camelus dromedarius) by magnetic resonance imaging, computed tomography, and cross-sectional anatomy

Research (Published online: 14-08-2020)

13. Description of normal head structures of the one-humped camel (Camelus dromedarius) by magnetic resonance imaging, computed tomography, and cross-sectional anatomy

Hassan Emam, Mohamed Aref, Ahmed Abdelbaset-Ismail, Ahmed Abdelaal, Shaimaa Gouda and Mohamed Gomaa

Veterinary World, 13(8): 1581-1587

ABSTRACT

Aim: This study was designed for the 1st time to describe the normal head structures of one-humped camel (Camelus dromedarius) using both magnetic resonance imaging (MRI) and computed tomography (CT) as well as cross-sectional anatomy.

Materials and Methods: Five fresh cadaver heads were collected from clinically normal camels and then subjected to T1-weighted MR and CT imaging. Afterward, these examined heads were transversely sliced to obtain seven crossing levels.

Results: The obtained structures per each crossing level were matched with their relevant sorted images of T1-weighted MRI and CT, then identified and labeled accordingly.

Conclusion: The data shown herein expand our knowledge of the normal head structures of the camel and could be used as a reference for ultimate diagnosis of the surgical affections of head using MRI and/or CT.

Keywords: camel, computed tomography, cross-leveling, head region, magnetic resonance imaging.

Seroprevalence study of peste des petits ruminants in sheep and goats in the northern region of India

Research (Published online: 13-08-2020)

12. Seroprevalence study of peste des petits ruminants in sheep and goats in the northern region of India

Vinayagamurthy Balamurugan, Bibitha Varghese, Kirubakaran Vinod Kumar, Dhanavelu Muthuchelvan, R. Dheeraj, Gurrappanaidu Govindaraj, Kuralayanapalya Puttahonnappa Suresh, Divakar Hemadri and Parimal Roy

Veterinary World, 13(8): 1573-1580

ABSTRACT

Background and Aim: Peste des petits ruminants (PPR) is a contagious, World Organization for Animal Health notifiable, economically important, transboundary morbilliviral disease of sheep and goats. Studying seroprevalence of PPR from different geographical areas under varying agro-climatic conditions may help in formulating effective and appropriate disease control strategies under the ongoing national PPR control program. The present cross-sectional study describes the prevalence of PPR virus antibodies in sheep and goats in the various epidemiological units in different states (Haryana, Himachal Pradesh [HP], Jammu and Kashmir [J&K], Punjab, Uttarakhand [UK], and Uttar Pradesh [UP]) of the northern region of India.

Materials and Methods: A total of 5843 serum samples (sheep [n=2463] and goats [n=3380]) were collected by stratified random sampling method from 322 epidemiological units in the studied region during 2017-2018 and tested for PPR virus (PPRV) antibodies by competitive ELISA.

Results: The results revealed that an overall seroprevalence of 44.05% (2574/5843) with 57.32%, 55.22%, 65.69%, 37.09%, 32.73%, and 29.35% prevalence of PPRV antibodies in small ruminants in Haryana, Punjab, UP, HP, J&K, and UK states, respectively. Further, Chi-squared test revealed an association of PPRV antibodies in goats (χ2=252.28, p<0.01) and sheep (χ2=192.12, p<0.01) across different states in the region.

Conclusion: The seroprevalence in majority of the epidemiological units (n=130) in sheep and goats in the studied region had <30%. This necessitates comprehensive, rigorous, continuous vaccination and active surveillance programs for few more years to achieve the desired 70% seroprevalence level of PPRV antibodies in population and to make the northern region of India, as PPR free zone.

Keywords: cross-sectional study, India, northern region, peste des petits ruminants, seroprevalence, sheep and goats.

Influence of antitox and Vitamin E-selenium on meat quality and safety in rabbits after 1,1-experimental dimethylhydrazine toxicosis

Research (Published online: 12-08-2020)

11. Influence of antitox and Vitamin E-selenium on meat quality and safety in rabbits after 1,1-experimental dimethylhydrazine toxicosis

Balgabay S. Maikanov, Mikhail V. Zabolotnykh, Laura T. Auteleyeva and Symbat P. Seidenova

Veterinary World, 13(8): 1567-1572

ABSTRACT

Aim: This study investigated the effects of antitox and Vitamin E-selenium on meat quality and safety in rabbits after experimental 1,1-dimethylhydrazine (1,1-DMH) toxicosis.

Materials and Methods: Experimental groups of rabbits weighing 1.5-2.5 kg each were kept in a vivarium under same controlled conditions (temperature 16-21°C, humidity 60-80%, 12/12 h light/dark cycle, noise level <85 dB) with free access to standard food containing 22.0% protein, 4.5% fat, and 4% fiber. The effect of a detoxifying mixture of antitox and Vitamin E-selenium on safety indicators (residual amount of 1,1-DMH) and quality indicators pre- and post-detoxification of the rabbits from 1,1-DMH was determined.

Results: After detoxification, the residual 1,1-DMH level decreased in all organs and tissues to <0.1 mg/kg. The nutritional value of meat increased by improving organoleptic, physical, and chemical parameters and the amino acid composition of protein.

Conclusion: The antitox+Vitamin E-selenium detoxifying mixture significantly decreases the residual 1,1-DMH level in organs and tissues of animals and increases the nutritional value of rabbit meat in subacute poisoning. The detoxifying mixture can also be used on animals grazing in territories adjacent to Proton-M launch sites for preventive and therapeutic purposes.

Keywords: 1,1-dimethylhydrazine, amino acid composition, detoxification, detoxifying mixture, meat, rabbits.

Protective effect of Mucuna pruriens against arsenic-induced liver and kidney dysfunction and neurobehavioral alterations in rats

Research (Published online: 12-08-2020)

10. Protective effect of Mucuna pruriens against arsenic-induced liver and kidney dysfunction and neurobehavioral alterations in rats

Preethi Concessao, Laxminarayana Kurady Bairy and Archana Parampalli Raghavendra

Veterinary World, 13(8): 1555-1566

ABSTRACT

Background and Aim: Intoxication of arsenic in rats is known to result in neurological effects as well as liver and kidney dysfunction. Mucuna pruriens has been identified for its medicinal properties. The aim of the study was to investigate the protective effect of aqueous seed extract of M. pruriens on sodium arsenite-induced memory impairment, liver, and kidney functions in rats.

Materials and Methods: The experiment was divided into short-term treatment (45 days) and long-term treatment (90 days), with each group divided into nine sub-groups consisting of six animals each. Sub-groups 1 and 2 served as normal, and N-acetylcysteine (NAC) controls, respectively. Sub-groups 3-9 received sodium arsenite in drinking water (50 mg/L). In addition, sub-group 4 received NAC (210 mg/kg b.wt) orally once daily, sub-groups 5-7 received aqueous seed extract of M. pruriens (350 mg/kg b.wt, 530 mg/kg b.wt, and 700 mg/kg b.wt) orally once daily and sub-groups 8 and 9 received a combination of NAC and aqueous seed extract of M. pruriens (350 mg/kg b.wt and 530 mg/kg b.wt) orally once daily. Following the treatment, the blood was drawn retro-orbitally to assess the liver (serum alanine transaminase [ALT], serum aspartate transaminase, and serum alkaline phosphatase) and kidney (serum urea and serum creatinine) functions. Learning and memory were assessed by passive avoidance test. Animals were sacrificed by an overdose of ketamine, and their Nissl stained hippocampal sections were analyzed for alterations in neural cell numbers in CA1 and CA3 regions.

Results: In the short-term treatment, groups administered with M. pruriens 530 mg/kg b.wt alone and combination of NAC + M. pruriens 350 mg/kg b.wt exhibited a significant improvement in memory retention, less severe neurodegeneration, and decrease in serum ALT levels. In long-term treatment, groups administered with M. pruriens 700 mg/kg b.wt alone and combination of NAC+M. pruriens 350 mg/kg b.wt, respectively, showed better memory retention, decreased neural deficits, and reduced levels of kidney and liver enzymes.

Conclusion: The seed extract of M. pruriens showed significant enhancement in memory and learning. The number of surviving neurons in the CA1 and CA3 regions also increased on treatment with M. pruriens. Serum ALT, serum urea, and serum creatinine levels showed significant improvement on long-term treatment with M. pruriens.

Keywords: hippocampus, kidney functions, liver functions, memory and learning, Mucuna pruriens, sodium arsenite.

In vitro anthelmintic activity of aqueous and ethanol extracts of Paraserianthes falcataria bark waste against Haemonchus contortus obtained from a local slaughterhouse in Indonesia

Research (Published online: 11-08-2020)

9. In vitro anthelmintic activity of aqueous and ethanol extracts of Paraserianthes falcataria bark waste against Haemonchus contortus obtained from a local slaughterhouse in Indonesia

Zein Ahmad Baihaqi, Irkham Widiyono and Wisnu Nurcahyo

Veterinary World, 13(8): 1549-1554

ABSTRACT

Aim: This study was conducted to determine the anthelmintic activity of aqueous and ethanol extracts of Paraserianthes falcataria bark against Haemonchus contortus.

Materials and Methods: Ethanol extract of bark (E.E.B.) waste and aqueous extract of bark (A.E.B.) waste of P. falcataria (at concentrations 0, 0.2, 0.4, 0.8, 1, 2.5, and 5%) and albendazole (2 mg/ml) as the positive control were placed in separate Petri dishes (50 mm). Twenty H. contortus worms were placed in Petri dishes and incubated at 37°C for 0.5, 1, 2, 3, 4, 5, 6, and 12 h. Mortality of each worm was ensured by pressing the body of the worm with a pair of tweezers and keeping it in lukewarm water for 5 min before declaring it dead. Mortality is defined as amount of death individuals and time of mortality of each worm was recorded. The parasites were then observed using scanning electron microscopy (SEM) at an accelerating voltage of 15 Kv. Statistical analysis was performed using SPSS 21.0 software, two-way ANOVA followed by Tukey's test to detect significant differences (p<0.05). The result was expressed as the mean ± standard deviation.

Results: The E.E.B. and A.E.B. of P. falcataria contained active compounds, such as tannin, alkaloid, flavonoid, saponin, steroid, and triterpenoid. E.E.B. had a higher content of phenol, while A.E.B. had a higher content of flavonoid. In this study, P. falcataria showed a significant effect (p=0.00) on H. contortus in vitro. E.E.B. (0.8%) was able to exterminate H. contortus completely after 6 h, more effective than A.E.B. (5%) while the positive control requires (2 mg/ml) after 2 h. SEM analysis of the worm treated with E.E.B. and A.E.B. showed damaged cuticle structure.

Conclusion: The aqueous and ethanol extracts of P. falcataria bark waste demonstrated anthelmintic activity against H. contortus.

Keywords: anthelmintic, bark, Haemonchus contortus, prevalence, scanning electron microscopy.