Effect of probiotic and Moringa oleifera extract on performance, carcass yield, and mortality of Peking duck

Research (Published online: 24-03-2022)

20. Effect of probiotic and Moringa oleifera extract on performance, carcass yield, and mortality of Peking duck

Widya Paramita Lokapirnasari, Bodhi Agustono, Mohammad Anam Al Arif, Lilik Maslachah, Evania Haris Chandra and Andreas Berny Yulianto

Veterinary World, 15(3): 694-700

ABSTRACT

Background and Aim: Antibiotics have been used as growth promoters in poultry. However, continuous and long-term antibiotics can cause resistance, suppress the immune system, and accumulate toxic residue. To overcome these problems, feed additives that are safe for livestock and health for humans are needed, including probiotics. Therefore, the study aimed to determine the effect of probiotics (Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus lactis, and Bifidobacterium spp.) and Moringa oleifera extract on performance (body weight gain, body weight, feed intake, feed efficiency, and feed conversion ratio [FCR]), carcass yield (carcass weight and percentage of carcass) and mortality of Peking duck.

Materials and Methods: This study used 48 Peking ducks, divided into four treatments and six replications. Each replication consisted of two ducks. The treatments were as follows: T0=control, T1=4 mL containing 1.2×108 CFU/mL of probiotic in drinking water, T2=4 mL containing M. oleifera extract in drinking water, and T3=2 mL containing 1.2×108 CFU/mL of probiotic in drinking water+2 mL containing M. oleifera extract in drinking water. The probiotics consist of L. acidophilus, L. casei, L. lactis, and Bifidobacterium spp. The data were statistically analyzed through analysis of variance. For the follow-up test, a multiple range test was conducted.

Results: There was no significant difference (p>0.05) between body weight, feed intake, and mortality treatments. By contrast, control and treatment showed a significant difference (p<0.05) on feed efficiency, FCR, body weight gain, carcass weight, and percentage of carcass weight. Results of body weight gain statistics showed no significant difference (p>0.05) between T0 and T1, but T0 and T1 showed a significant difference with T2 and T3. The results of the feed efficiency statistic showed no significant difference (p>0.05) between T0, T1, and T2, but there was a significant difference between T0, T1, and T3. Feed efficiency at T2 showed no significant difference with T3, T1, and T0. The results of the FCR statistic showed no significant difference (p>0.05) between T0, T1, and T2, but there was a significant difference between T0, T1, and T3. FCR at T2 showed no significant difference with T3, T1, and T0. The carcass weight statistic showed no significant difference (p>0.05) between T0, T1, and T3, but there was a significant difference between T0 and T2. T2 showed no significant difference with T1 and T3. The carcass percentage statistic showed no significant difference (p>0.05) between T0 and T1, but T0 and T1 showed a significant difference (p<0.05) with T2 and T3.

Conclusion: Based on the study results, it can be concluded that the use of a combination of probiotics (L. acidophilus, L. casei, L. lactis, and Bifidobacterium spp.) and M. oleifera extract can increase the production performance of Peking ducks and is safe for ducks' health.

Keywords: health, probiotic, Moringa oleifera, Peking duck.

The application of ascorbic acid as a therapeutic feed additive to boost immunity and antioxidant activity of poultry in heat stress environment

Review (Published online: 24-03-2022)

19. The application of ascorbic acid as a therapeutic feed additive to boost immunity and antioxidant activity of poultry in heat stress environment

Truong Van Hieu, Budi Guntoro, Nguyen Hoang Qui, Nguyen Thi Kim Quyen and Farid Akbar Al Hafiz

Veterinary World, 15(3): 685-693

ABSTRACT

Ascorbic acid, widely known as vtamin C, is an essential nutrient for animals such as poultry. Ascorbic acid in poultry feed improves animal health and thus increases the growth performance of birds. Ascorbic acid can be used in the form of synthetic products or can be naturally obtained from fruits and plants. It is soluble in water and can be easily administered in drinking water and the diet. Poultry can synthesize ascorbic acid in the body. However, the performance of the animals can be improved by adding ascorbic acid to their diet. In addition, ascorbic acid is called an antioxidant and an anti-inflammatory. This increases their resistance to disease during the transition season. Ascorbic acid supplementation positively affects the stress response, especially during the dry season in tropical countries. Furthermore, supplementing ascorbic acid in the poultry's diet improves resistance to diseases, regulates stress, and helps in the body's oxidation process. Ultimately, this enhances the laying rate, egg hatch performance, and higher poultry productivity. For layers at the end of the laying period, it helps increase the quality of the eggshell and reduces the proportion of broken eggs. Ascorbic acid has a strong relationship with other vitamins such as vitamin E and other substances such as zinc, safflower oil, folic acid, and a fibrous diet. This review aims to synthesize all the information of ascorbic acid in the poultry's diet, thereby providing the general role of ascorbic acid for the poultry industry.

Keywords: antioxidant, ascorbic acid, immune system, poultry production.

Early hip laxity screening and later canine hip dysplasia development

Research (Published online: 24-03-2022)

18. Early hip laxity screening and later canine hip dysplasia development

Ana Santana, Sofia Alves-Pimenta, Pedro Franco-Gonçalo, Lio Gonçalves, João Martins, Bruno Colaço and Mário Ginja

Veterinary World, 15(3): 679-684

ABSTRACT

Background and Aim: Passive hip laxity (PHL) is considered the primary risk factor for canine hip dysplasia (HD) and is estimated, in stress hip radiographs, using the distraction index (DI). The study aimed to associate the early PHL using the hip Distractor of University of Trás-os-Montes and Alto Douro (DisUTAD) and the late HD grades.

Materials and Methods: A total of 41 dogs (82 hips) were submitted to a follow-up study. First, between 4 and 12 months of age, dogs were radiographed using the DisUTAD hip distractor and were determined the DI for each hip joint. Then, after 12 months of age, dogs were reevaluated for HD using the conventional hip ventrodorsal projection and hips were evaluated for HD using the Fédération Cynologique Internationale (FCI) scoring system.

Results: Hips of dogs' in the second examination with FCI grades of A (n=28), B (n=11), C (n=22), and D and E (n=21) had an early DI of 0.32±0.1, 0.38±0.08, 0.50±0.12, and 0.64±0.11, respectively. Statistical analysis using the general linear model univariate, with the DI as dependent variable and the FCI grades, side and sex as fixed factors, and the post hoc Bonferroni correction test showed significant differences among FCI grades (p<0.05).

Conclusion: These results show the association between early DI and the late FCI HD grades and the DisUTAD is recommended for the early canine HD diagnosis.

Keywords: canine hip dysplasia, distraction index, hip distractor DisUTAD, hip laxity.

Hematological indices and their correlation with glucose control parameters in a prediabetic rat model

Research (Published online: 24-03-2022)

17. Hematological indices and their correlation with glucose control parameters in a prediabetic rat model

Desak Gede Budi Krisnamurti, Erni H. Purwaningsih, Tri Juli Edi Tarigan, Vivian Soetikno and Melva Louisa

Veterinary World, 15(3): 672-678

ABSTRACT

Background and Aim: Chronic hyperglycemia in prediabetic individuals would progress to diabetes and lead to several systemic disruptions, including hematological parameters. This study aimed to investigate the correlation between prediabetes and hematological indices in a prediabetic rat model.

Materials and Methods: Eighteen male rats were randomly divided into two groups of nine. Prediabetes was induced in nine rats by a 3-week high-fat and high-glucose diet, followed by low-dose streptozotocin (STZ) injection (30 mg/ kg body weight). The oral glucose tolerance test was performed, and the fasting blood glucose (FBG) and insulin levels were measured 72 h after STZ administration. The control group of nine rats was given standard diets. At the end of the 3rd week, the animals fasted overnight before blood collection. Blood samples were drawn and used for the analysis of the FBG and fasting insulin levels and glycated albumin to define prediabetes criteria before hematology analysis.

Results: We found a significant increase in the FBG and insulin levels in the prediabetic versus the control group. There were decreases in red blood cells, hemoglobin, and hematocrit levels and red cell distribution in prediabetic rats versus the control. At the same time, a significant increase in the platelet count was observed in the prediabetic group. There was a positive correlation between FBG and lymphocytes and neutrophil-lymphocyte ratio in prediabetic rats. On the other hand, we found a negative correlation between white blood cell count and glycated albumin.

Conclusion: Correlations were found in several hematological parameters in the prediabetic rat models. The changes in hematological indices in prediabetic rats may be further used as a valuable indicator of glycemic control.

Keywords: diabetes mellitus, high-fat and high-glucose diet, hyperglycemia, neutrophil-lymphocyte ratio, streptozotocin.

The public health issue of antibiotic residues in food and feed: Causes, consequences, and potential solutions

Review (Published online: 23-03-2022)

16. The public health issue of antibiotic residues in food and feed: Causes, consequences, and potential solutions

Mbarga Manga Joseph Arsène, Anyutoulou Kitio Linda Davares, Podoprigora Irina Viktorovna, Smolyakova Larissa Andreevna, Souadkia Sarra, Ibrahim Khelifi and Das Milana Sergueïevna

Veterinary World, 15(3): 662-671

ABSTRACT

Antibiotics are among the essential veterinary medicine compounds associated with animal feed and food animal production. The use of antibiotics for the treatment of bacterial infections is almost unavoidable, with less need to demonstrate their importance. Although banned as a growth factor for a few years, their use in animals can add residues in foodstuffs, presenting several environmental, technological, animal health, and consumer health risks. With regard to human health risks, antibiotic residues induce and accelerate antibiotic resistance development, promote the transfer of antibiotic-resistant bacteria to humans, cause allergies (penicillin), and induce other severe pathologies, such as cancers (sulfamethazine, oxytetracycline, and furazolidone), anaphylactic shock, nephropathy (gentamicin), bone marrow toxicity, mutagenic effects, and reproductive disorders (chloramphenicol). Antibiotic resistance, which has excessively increased over the years, is one of the adverse consequences of this phenomenon, constituting a severe public health issue, thus requiring the regulation of antibiotics in all areas, including animal breeding. This review discusses the common use of antibiotics in agriculture and antibiotic residues in food/feed. In-depth, we discussed the detection techniques of antibiotic residues, potential consequences on the environment and animal health, the technological transformation processes and impacts on consumer health, and recommendations to mitigate this situation.

Keywords: animal breeding, antibiotic resistance, antibiotics residues, food and feed, public health.

First identification of Mycobacterium avium subsp. paratuberculosis in wild ruminants in a zoo in Mexico

Research (Published online: 23-03-2022)

15. First identification of Mycobacterium avium subsp. paratuberculosis in wild ruminants in a zoo in Mexico

A. L. Hernández-Reyes, G. Chávez-Gris, E. Maldonado-Castro, L. E. Alcaraz-Sosa and M. T. Díaz-Negrete

Veterinary World, 15(3): 655-661

ABSTRACT

Background and Aim: Paratuberculosis (PTB) is an infectious disease that induces chronic enteritis in ruminants. It is caused by Mycobacterium avium subsp. paratuberculosis (MAP). In this study, we evaluated the presence of MAP using bacteriological, molecular, and anatomopathological studies, based on the clinical suspicion of PTB in a zoo, in an area housing 10 scimitar-horned oryx (Oryx dammah), five giraffes (Giraffa camelopardalis), and three blue wildebeests (Connochaetes taurinus).

Materials and Methods: From November 2016 to June 2017, fecal samples were collected from individuals of the three species on four occasions, resulting in a total of 56 fecal samples. In addition, five small intestine samples were collected from the necropsies of three adult scimitar-horned oryx females and two oryx calves. MAP identification was performed through isolation in Herrold's medium with egg yolk, mycobactin, and sodium pyruvate, Ziehl–Neelsen staining, IS900 polymerase chain reaction (IS900 PCR), and anatomopathological examination of intestine samples.

Results: Diffuse granulomatous enteritis with abundant acid-fast bacilli was found in two out of five intestine samples from adult scimitar-horned oryx females. MAP was isolated in 7/56 (12.5%) of the fecal samples from four scimitar-horned oryx, one giraffe, and two wildebeest samples. Two out of 5 (40%) samples obtained from scimitar-horned oryx tested positive. IS900 PCR yielded five positive samples (two fecal samples and three small intestine samples). MAP isolates were classified as Type C (Cattle) using type-specific PCR.

Conclusion: These results demonstrated the presence of MAP in the area evaluated and indicated the importance of both sampling live animals and conducting postmortem examinations. The use of bacteriological and histopathological diagnostic techniques demonstrated in this study will provide insight into the health status and prevalence of paratuberculosis in wild ruminants under human care.

Keywords: histopathology, IS900, isolation, paratuberculosis, scimitar-horned oryx, zoo animals.

Study of fecal glucocorticoid metabolites in captive Asian elephants in Kanchanaburi Province, Thailand

Research (Published online: 23-03-2022)

14. Study of fecal glucocorticoid metabolites in captive Asian elephants in Kanchanaburi Province, Thailand

Weerapun Nokkaew, Apiradee Intarapuk, Apichaya Sakulthai, Worawidh Wajjwalku and Nikorn Thongtip

Veterinary World, 15(3): 647-654

ABSTRACT

Background and Aim: Over the past two decades, the number of elephant camps in Thailand has increased considerably, and captive elephants have become more popular within the tourism industry. Tourist activities involving elephant exhibitions and trekking potentially affect animal health and welfare. This study aimed to investigate the relationships between a novel stress biomarker, fecal glucocorticoid metabolites (fGCM), and various factors (sex, age, weather season, tourist season, and elephant usage patterns), monitoring the fGCM concentration during and after trekking activities ceased.

Materials and Methods: Fecal samples of 20 captive Asian elephants from two camps in Kanchanaburi Province were collected monthly for 1 year. The fGCM concentrations were measured using enzyme immunoassay and evaluated relative to individual demography, season, and tourist trekking activity. The mean differences of fGCMs concentrations were compared by analysis of variance and t-test statistics according to data types with p<0.5.

Results: Significant differences in mean fGCM concentrations were found between age categories (p=0.001), trekking and non-trekking animals (p=0.039), and during and after trekking (p=0.023). The mean fGCM concentration of elephants aged during 0-44 years (136.7 ng/g) was significantly higher than for animals over 44 years old (107.7 ng/g), and the elephant trekking group (144.9 ng/g) was significantly higher than the other group (124.7 ng/g). Within the trekking group, the mean fGCM concentrations gradually declined to 129.13 ng/g within 8 months of trekking cessation.

Conclusion: Elephant's ages and activities co-influenced the variance of fGCM concentrations. In addition, permanent tourist activity, especially trekking, can increase elephant stress. This study's findings can be applied to the health status monitoring of captive elephants and result in improved animal welfare.

Keywords: captive elephant, fecal glucocorticoid, hormone, stress, trekking.