Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models

Research (Published online: 10-07-2021)

9. Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models

Sri Ratna Dwiningsih, Soehartono Darmosoekarto, Hendy Hendarto, Erry Gumilar Dachlan, Fedik Abdul Rantam, Sunarjo Sunarjo, I. W. Arsana Wiyasa and Widjiati Widjiati

Veterinary World, 14(7): 1788-1796

ABSTRACT

Background and Aim: Endometriosis affects the ovaries and causes a decrease in the oocyte quality during endometrial receptivity. During the development of ovarian follicles, paracrine communication occurs between granulosa cells and oocytes. This study was conducted to determine the effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha (TNF-α) receptor 1 (TNFR1) expression, granulosa cell apoptosis, and folliculogenesis in endometriosis mouse models.

Materials and Methods: This study involved 42 female mice, which were divided into three groups: Healthy mice (T0), endometriosis mice without transplantation (T1), and endometriosis mice with bone marrow mesenchymal stem cell transplantation (T2). The mice were injected intraperitoneally with endometrial fragments (200 μL) to become endometriosis models. On day 15, the endometriosis models received mesenchymal stem cells. Sample collection was performed on day 29. Granulosa cell apoptosis and TNFR1 expression were examined using immunohistochemical staining, and folliculogenesis was assessed using hematoxylin and eosin staining of ovary samples. The data obtained from both examinations were statistically analyzed using Statistical Package for the Social Sciences.

Results: The results showed that TNFR1 expression is significantly decreased in T2 (p<0.004). The apoptosis of granulosa cells was lower in T2 (p<0.000). The primary, secondary, and graafian follicle counts in T2 were significantly increased.

Conclusion: Bone marrow mesenchymal stem cell transplantation in endometriosis mouse models can reduce TNFR1 expression and granulosa cell apoptosis and improve folliculogenesis.

Keywords: apoptosis, bone marrow mesenchymal stem cells, endometriosis, folliculogenesis, granulosa cells, tumor necrosis factor-alpha receptor 1.

A comparative study on the effects of boiling and ultrasonication on radical scavenging activity, casein particle size, and whiteness of milk

Research (Published online: 09-07-2021)

8. A comparative study on the effects of boiling and ultrasonication on radical scavenging activity, casein particle size, and whiteness of milk

Tanmay Hazra, Rohit Sindhav, Ch. V. K. Sudheendra, Mitul Bumbadiya, Radhika Govani and Vimal Ramani

Veterinary World, 14(7): 1784-1787

ABSTRACT

Background and Aim: Different processing treatments affect the functional properties of milk. This study aimed to evaluate the effects of boiling and ultrasonication on radical scavenging activity, micellar casein particle size, and the whiteness of milk.

Materials and Methods: Milk was subjected to boiling and ultrasonication treatments. Then, the micellar casein size and the whiteness of the milk were evaluated using L-Value- intensity of whiteness and the radical scavenging capacity of the milk was evaluated using 1,1-diphenyl-2-picrylhydrazyl method.

Results: The radical scavenging activity of the milk was found to be reduced during the different processing treatments, but this decrease was non-significant for the ultrasonication treatment (p>0.05). However, a significant reduction in radical scavenging activity (p<0.05) was observed for the boiled milk. Micellar casein size reduction was observed after both treatments, and boiling had a significant effect (p<0.05) on the micellar casein particle size. We found that the whiteness of skim milk was significantly decreased after boiling treatment, but was not significantly decreased after ultrasonication. Ultrasonication had a non-significant effect on the whiteness of ultrasonicated milk which was observed.

Conclusion: Ultrasonicated milk had a very non-significant effect on the antioxidant activity (radical scavenging activity) of milk, whereas the effect of boiling was significant. Ultrasonication treatment increases the shelf-life of milk while retaining its bioactive properties.

Keywords: antioxidant, radical scavenging activity, skim milk, ultrasonication.

Development of rat metabolic syndrome models: A review

Review (Published online: 07-07-2021)

7. Development of rat metabolic syndrome models: A review

Shirly Gunawan, Ahmad Aulia and Vivian Soetikno

Veterinary World, 14(7): 1774-1783

ABSTRACT

Metabolic syndrome (MetS) has become a global problem. With the increasing prevalence of MetS worldwide, understanding its pathogenesis and treatment modalities are essential. Animal models should allow an appropriate representation of the clinical manifestations of human conditions. Rats are the most commonly used experimental animals for the study. The development of a proper MetS model using rats will contribute to the successful application of research findings to the clinical setting. Various intervention methods are used to induce MetS through diet induction with various compositions, chemicals, or a combination of both. This review will provide a comprehensive overview of several studies on the development of rat MetS models, along with the characteristics of the clinical manifestations resulting from each study.

Keywords: high-fat diet, high-fructose diet, high-sucrose diet, metabolic syndrome, rat models.

Molecular identification of fluoroquinolone resistance in Salmonella spp. isolated from broiler farms and human samples obtained from two regions in Colombia

Research (Published online: 07-07-2021)

6. Molecular identification of fluoroquinolone resistance in Salmonella spp. isolated from broiler farms and human samples obtained from two regions in Colombia

María Paula Herrera-Sánchez, Rafael Enrique Castro-Vargas, Luz Clemencia Fandiño-de-Rubio, Roy Rodríguez-Hernández and Iang Schroniltgen Rondón-Barragán

Veterinary World, 14(7): 1767-1773

ABSTRACT

Background and Aim: Salmonella is one of the most common foodborne pathogens, the emergence of antibiotic-resistant strains of which is increasing. The aim of this study was to phenotypically and genotypically characterize the fluoroquinolone resistance of Salmonella isolates from broiler and humans in two regions of Colombia.

Materials and Methods: Salmonella strains (n=49) were evaluated. The phenotype of antibiotic resistance was assessed by an automated method and agar diffusion method, as well as the presence of the quinolone resistance genes qnrA, qnrB, qnrC, qnrD, qnrS, and aac(6')-Ib as determined by polymerase chain reaction.

Results: Strains were resistant to ciprofloxacin (75%), levofloxacin (57.1%), and enrofloxacin (38.8%). Molecular identification showed that 24 out of 49 strains possessed the qnrB gene (48.9%), while only one isolate from the Santander region possessed the aac(6')-Ib gene. Regarding Class 1 integron, it was present in 11 out of the 49 strains (22.44%).

Conclusion: This study reports the presence of the gene qnrB as well the presence of Class 1 integrons in broiler Salmonella isolates, which may contribute to the resistance to fluoroquinolones.

Keywords: antibiotic resistance, broiler, resistance genes, Salmonella.

Etiopathological and hematobiochemical profiles in goats with gastrointestinal disorders

Research (Published online: 06-07-2021)

5. Etiopathological and hematobiochemical profiles in goats with gastrointestinal disorders

Sunena Nayak, Prasana Kumar Rath, Susen Kumar Panda, Bidyut Prava Mishra, Rajshree Mishra and Shuvranshu Sekhar Biswal

Veterinary World, 14(7): 1760-1766

ABSTRACT

Background and Aim: Gastrointestinal (GI) disorders in small ruminants limit production efficiency and productivity growth in the livestock sector, thereby directly preventing farmers from augmenting their income. This study aimed to provide detailed insight into the etiology, hematobiochemical parameters, and epidemiological risk factors of GI disorders in goats and to determine the pathology associated with the disorders.

Materials and Methods: Over the period of 2018-2019, 500 goats in and around Bhubaneswar, Odisha, India, were screened for GI disorders based on clinical signs. Blood samples from the control (n=10) and treatment (n=25) groups were collected for both hematological and serum biochemical alterations. Fecal examinations (n=220) were conducted for parasitic, bacterial, and virological assessments. Detailed necropsy and histopathological evaluations were conducted on 27 goats.

Results: The GI disorder prevalence rate and mortality rate among the 500 goats analyzed were 44.4% and 12.27%, respectively. Chi-square analysis showed a significantly higher occurrence of GI disorders among the goats that were between 6 months and 1.5 years old (58.72%), were of the Ganjam breed (45.49%), had a poor body condition (71.11%), and were housed with an earthen floor (55.22%). The most common etiological risk factor observed was parasitic infection (65.45%), followed by bacterial (18.18%) and mixed infection (9.54%). Blood analysis showed neutrophilia and eosinophilia in infected goats, in addition to anemia; significant decreases in total protein, globulin, albumin, and glucose levels; and significant increases in aspartate transaminase and alanine aminotransferase levels. The major histopathological findings were infiltration of mononuclear cells and desquamation of the intestinal and ruminal mucosa.

Conclusion: Stakeholders should focus not only on parasitic infections and other important etiological risk factors for GI disorders in goats but also on proper farming management practices to help enhance the income of farmers. The hematobiochemical alterations and pathomorphological changes reported in this study can be used by field veterinarians as guidelines for clinical evaluation and disease severity assessment.

Keywords: diarrhea, goat, gastrointestinal disorders, hematobiochemical.

Technology for evaluating the ability of probiotic strains to accumulate copper ions in vitro and in vivo using Bacillus bacteria

Research (Published online: 06-07-2021)

4. Technology for evaluating the ability of probiotic strains to accumulate copper ions in vitro and in vivo using Bacillus bacteria

Aleksey Sizentsov, Elena Salnikova, Yaroslav Sizentsov, Sergey Peshkov, Elena Barysheva, Olga Naumenko and Natalia Romanenko

Veterinary World, 14(7): 1752-1759

ABSTRACT

Background and Aim: Microorganisms of the genus Bacillus comprising probiotics could have an antitoxic effect that is manifested in the active excretion of toxic substances from the body, as well as heavy metals. This study was conducted to assess the effects of copper ions on probiotic strains based on the members of the genus Bacillus in vitro and in vivo.

Materials and Methods: The following probiotic preparations were selected for this experiment: "Sporobacterin," "Bactisubtil," and "Vetom-2." Sporobacterin liquid, manufactured by Bakoren LLC, is based on Bacillus subtilis 534. Bactisubtil, manufactured by Marion Merrell Dow, Inc., is based on Bacillus cereus 5832. The first part of the experiment (in vitro) was conducted to assess the influence of copper ions on the growth and bioaccumulating ability of probiotic strains. The second part of the experiment (in vivo) was conducted to assess the influence of copper ions on the body of laboratory animals and to determine the ability of probiotic strains to remove copper ions from the body of laboratory animals. Statistical analysis was performed using Statistical Package for the Social Sciences, calculating the average value (M), standard deviation (σ), and standard deviation error (m). p<0.05 was used to denote statistical significance.

Results: In the previous studies, we found the presence of pronounced sorption characteristics of representatives of both the intestinal microbiome and probiotic strains based on them. In this study, we have studied the prospects of using physiological and adaptive mechanisms of resistance of transient probiotic strains in the system of correction of the elemental status of the animal body due to excessive intake of copper ions into the body. The advantage of their use is due to not only sorption but also the high levels of elimination of complexes accumulated on the surface. Analyzing the data, we can state the following. The excessive content of copper ions inhibits the activity of all microorganisms, and the presence of CuSO4 in the nutrient medium reduces the exponential growth phase by 6 h for B. licheniformis. The analysis of data on the bioaccumulating property of the probiotic strains under study shows that B. cereus (part of Bactisubtil) had the most pronounced sorbing effect with the level of accumulation of 23.96%.

Conclusion: We found that probiotic preparations do not affect biochemical indices of blood and biotissues (the muscle and bone tissue, and the cutaneous covering). As a result of determining the ability of Bacillus bacteria comprising the probiotics under this study to accumulate heavy metals by measuring their concentration in the tissues of laboratory animals, the preparations contribute to reducing the toxic effects of copper ions on the body. The cutaneous covering has the greatest accumulation property relative to copper ions. The most effective probiotic under this study in copper ion poisoning was Bactisubtil, and the least effective was Vetom-2.

Keywords: Bacillus, bioaccumulation, copper, in vitro, in vivo, intoxication, ions, probiotics, technology.

The presence of pathogens and heavy metals in urban peregrine falcons (Falco peregrinus)

Research (Published online: 03-07-2021)

3. The presence of pathogens and heavy metals in urban peregrine falcons (Falco peregrinus)

Ewelina Pyzik, Marta Dec, Dagmara Stępień–Pyśniak, Agnieszka Marek, Jose Louis Valverde Piedra, Agnieszka Chałabis-Mazurek, Klaudiusz Szczepaniak and Renata Urban-Chmiel

Veterinary World, 14(7): 1741-1751

ABSTRACT

Background and Aim: Wild birds raised in urban environments may be exposed to many negative factors, including biological and chemical toxic elements. The aim of the study was to assess the occurrence of bacteria and parasites in wild birds, based on the example of the peregrine falcon (Falco peregrinus) as a potential indicator of bacterial drug resistance genes. Toxicological contamination was also analyzed to determine the impact of urbanized areas on this predatory species, in terms of its health, welfare, and survival in urban environments.

Materials and Methods: The samples consisted of down feathers and fresh feces obtained from seven falcon chicks (during obligatory veterinary examination) reared in two nests located in the Lublin region (Lublin and Pulawy). Bacteria and parasites were isolated directly from feces by classical microbiological methods, polymerase chain reaction, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS). The down feathers and feces of birds were used for toxicological testing by plasma inductively coupled plasma MS to assess the concentrations of selected heavy metals (cadmium [Cd], lead [Pb], arsenic [As], zinc [Zn], and copper [Cu]).

Results: The study revealed the presence of a diverse microbiome in the falcon chicks, among which Escherichia coli, Enterococcus spp., and Staphylococcus spp. bacteria and parasites of the genus Caryospora were dominant. The presence of drug resistance genes was also confirmed among the pathogens. The toxicological analysis found high concentrations of toxic heavy metals, including Cd, Pb, As, and Zn, in the downy feathers and feces of peregrine chicks.

Conclusion: Predatory free-living birds living in urban environments not only can be infected with various pathogens but may also show contamination with heavy metals, which could influence their natural resistance, condition, and welfare.

Keywords: bacteria, falcons, health status, toxic elements, welfare.